• Archives of Pharmacal Research
• /
• v.27 no.9
• /
• pp.901-905
• /
• 2004

A rapid, sensitive and selective hydrophilic interaction liquid chromatography-tandem mass spectrometric(HILIC-MS/MS) method for the determination of tiapride in human plasma was developed. Tiapride and internal standard, metoclopramide were extracted from human plasma with dichloromethane at basic pH and analyzed on an Atlantis HILIC silica column with the mobile phase of acetonitrile-ammonium formate (190 mM, pH 3.0) (94：6, v/v). The ana-Iytes were detected using an electrospray ionization tandem mass spectrometry in the multi-ple-reaction-monitoring mode. The standard curve was linear (r＝0.999) over the concentration range of 1.00-200 ng/mL. The coefficient of variation and relative error for intra- and inter-assay at three QC levels were 6.4∼8.8％ and -2.0∼3.6％, respectively. The recoveries of tiapride ranged from 96.3 to 97.4％, with that of metoclopramide (internal standard) being 94.2％. The lower limit of quantification for tiapride was 1.00 ng/mL using 1 00 $\mu$L of plasma sample.

• Geotechnical Engineering
• /
• v.3 no.1
• /
• pp.65-76
• /
• 1987

This study has been carried out as a basic course for the analysis of foundation deformations based on the Cap model using the finite element methods. Material parameters should firstly be determined in order to use the Cap model for numerical solution. Associated with the fact described above, a method determining the soil parameters is suggested using algorithm for numerical ana])isis from raw truly triaxial compression laboratory test data of Pueblo.Colorado sand by Zaman, et at. (1982) More specifically, the change of soil parameters Is thoroughly examined by weighting the data obtained from CTC and RTE tests, respectively. The main results obtained are as follows; 1. The obtained values of parameters (E, V and 2) are same irrespective of data obtained from various kind of tests. 2. The values of the other parameters are dependent on data used. 3. The determination of parameters is little affected by the weighting factor.

• The Journal of Korean Medicine
• /
• v.27 no.1 s.65
• /
• pp.23-35
• /
• 2006

Objectives : This study was conducted to investigate the inhibitory effects of Gaejibokryunghwan on cell proliferation in HeLa cells. Methods : Human uterine cervical carcinoma HeLa cells were cultured in the 1%, 5% and 10% concentration of Gaejibokryunghwan extract solution. All three were cultured for 24 hours, 48 hours and 72 hours each, to examine the inhibitory effects of Gaejibokryunghwan. Afterwards, we drew out the effect of Gaejibokryunghwan extract solution by making 5 analysis. First analysis was to measure the proliferation rate of cells. Second was FACS analysis. Third was to estimate the activity or caspase-3. Fourth, we used XTT assay to analyze the activation or cells. Ana lastly, a molecular biological method was used to determine activation of MAP kinase in the HeLa cells. Results : After 24, 48 and 72 hours cultivation, the proliferation of HeLa cells showed the dose-dependent decrease in all Gaejibokryunghwan extract solution groups compared to the control group. In the FACS analysis, Gaejibokryunghwan extract solution groups showed increased caspase expression compared to the control group, except for the group for 48 and 72 hours in 1 % concentrate. Caspase-3 activities were increased in all, except tile group cultured for 24 hours in 5% concentrate and the groups cultured for 48 hours in 1% and 5% concentrate. In the XTT study, 1% Gaejibokryunghwan extract solution groups showed increase compared to the control group, but other Gaejibokryunghwan extract solution containing groups showed significant decrease compared to the control after 24, 48 and 72 hours of cultivation. The expressions of MAP kinase were decreased in all Gaejibokryunghwan extract solution containing groups compared to the control group after 24, 48 and 72 hours of cultivation. Conclusions : From this study, we could suggest that Gaejibokryunghwan be available to the inhibition of proliferation of human cervical carcinoma cell line, HeLa cells in vitro.

• Journal of the Korean Society of Clothing and Textiles
• /
• v.10 no.3
• /
• pp.27-36
• /
• 1986

The purpose of this study was, to estimate the detergency of commercial detergent and to examine the interrelationship between detergency and detergent characteristics, detergent concentration, fiber characteristics, and Rolling-up phenomenon which is the major removal mechanism of oily soil. A mixture of oleic acid-olive oil was used as oily soil. The detergency was estimated by analysis of oleic acid on cotton ana polyester fabrics before and after washing by means of liquid scintillation counting. The Rolling-up of oily soil from PET film was observed and change of contact angle and removal time were measured. The results were as follows ; 1. In the case of soaps and powder synthetic detergents, the optimum concentrations we-re about $0.2\~0.3\%$. And detergencies of liquid synthetic detergents were considerably low, and the detergency was continually increased up with increasing concentration to $0.5\%$, which seemed to be caused by the lower pH than that of soap and powder synthetic deter-gent solutions. 2. As the effect of external or internal fiber structure; the detergency of cotton was lower than that of polyester. 3. In the observation of Rolling-up, the contact angle increased and the Rolling-up time became shorter with increasing detergent concentrations ($0.05\~0.5\%$). And it was confirmed that detergency was increased with Rolling-up effect. In addition, the study on the actual laundry condition was studied using the questionaire. From the results, about $76\%$ of households used the concentration of detergent roughly or excessively and housewives were highly concerned on the recommended dose, but grade of practice were very low.

• Polymer(Korea)
• /
• v.24 no.6
• /
• pp.829-836
• /
• 2000

In order to recyle the FRP waste from SMC bathtubs and rock-crush sludge obtained as a byproduct of stones, the composite consisting of the FRP and rock-crush sludge and the unsaturated polyester matrix resin were prepared. To enhance the interfacial bonding force between the reinforcements and the matrix resin, the rock-crush sludge was treated with silane coupling agent, ${\gamma}$-methacryloxypropyltrimethoxysilane (${\gamma}$-MPS) and their mechanical properties and interface phenomena were examined. The flexural modulus of the composite containing 10 wt% rock-crush powder treated with 3 wt% silane coupling agent showed the maximum value. And also the initial thermal degradation temperature of composites were in the range of 352~359$^{\circ}C$. From these results, we observed that the weight loss of composites was almost constant regardless of the concentration of silane coupling agent. It is confirmed that the interface of the composites containing filler treated with ${\gamma}$-MPS was improved in that there were no pull-out phenomena between the reinforcement and matrix resin.

• Journal of the Korean Society of Clothing and Textiles
• /
• v.16 no.3 s.43
• /
• pp.325-333
• /
• 1992

The objectives of this study were 1) to investigate the clothing needs of physically han- dicapped children, 2) to present the basic data to make the slacks patterns which meet the specific needs of physically handicapped children under study. Questionnaires were administered to 38 mothers of handicapped children and the subjects for the wear test were five handicapped children using wheelchair. The fabrics used for slacks wear test were: muslin woven with cotton, stretchable jean woven and double jersey knitted with cotton and polyester fabrics. The results obtained from this study were as follows: 1) The majority of the handicapped children were wearing ready·made-garments and their mothers considered the fabric elasticity first. 2) The slacks wear test indicated that the slacks ease of 8 cm should be added above the natural waist line in the back for the wheelchair bound children. 3) In a sitting posture on the wheelchair, $3\~4$ cm should be cut below the natural waist line in the front but in a standing posture, $1\~2$ cm should be cut below the natural waist line in the front. 4) The double jersey (cotton/polyester) slacks was highly estimated of ease ana length of slacks in a sitting posture. The stretchable jean (cotton/spandex) slacks was estimated high of appearance but muslin slacks (cotton) was less estimated of ease and appearance of slacks.

• Korean journal of food and cookery science
• /
• v.2 no.1
• /
• pp.59-64
• /
• 1986

This study was designed to revive the disappeared Younsa and to investigate the optimum processing condition and characteristics of Younsa nade of the glutinous rice powder. Agitating time of base mixture, thickness of Younsa base, and frying time were examined as the influencing factors in Younsa processings. The textural parameters of Younsa were determined by sensory cvaluation and hardness of Younsa was measured by Rheometer. The results were found as follows; 1. In the sensory evaluation, 1) The Younsa base agitated for 10 minutes by agitating machine was favorable. It, however, did not showed any significant difference among products prepared under varying agitaing time-10 minutes, 20 minutes, and 30 minutes. (p<0.05) 2) The thinnest Younsa base (5 cm$\times$4cm, 1.1-1.5g) showed the most favorable quality and the second quality went to 5cm$\times$4cm, 1.6-2.0g, the third 5cm$\times$4cm, 2.1-2.5g, the fourth 5 cm$\times$4 cm, 2.6-3.0g. (p<0.05) 3) In examination of varying frying time, the optimal time for frying Younsa was 12 minutes was 12 minutes in $150 ^{\circ}C$. However, the quality was found no significant difference between 12 minutes's fried Younsa and 14 minutes's. (p<0.05). 2. The hardness of the most favorable Younsa, agitated for 10 minutes, the thinnest (5 cm$\times$4 cm, 1.1-1.5g), ana fried for 12 minutes in $150^{\circ}C$ was 0.59-0.73 kg/wt measured by Rheometer. The thickest Younsa base (5 cm$\times$4 cm, 2.6-3.0g) is the hardest (1.26 kg/wt). The most optimal conditions in this experiment were found the same ones which was written in historical literature.

• Korean Journal of Fisheries and Aquatic Sciences
• /
• v.16 no.3
• /
• pp.197-201
• /
• 1983

Underwater ambient noise level was measured at two points near the Youngil Bay. The environmental characteristics depend upon oceanographic conditions of sound propagation ana its implication on the source of ambient noise. Some noise sources were estimated, and the effect of the oceanographic conditions on the noise level variation had been considered. The results were as follows : 1) At the nearshore station of Youngil Bay, the ambient noise level in the near bottom(45m) was lower than that of the near surface(10m) by 15dB. This difference was due to spherical spreading from the upper to the lower layer. 2) At the open sea station which is located outside of the thermal front existing near the Youngil Bay, the ambient noise level of the upper layer(20m) was higher than that of the lower layer (100m) by $8{\sim}12dB$ below 50Hz and $15{\sim}23dB$ above 50Hz. 3) Above 60Hz the ambient noise level at the nearshore station was higher than that of the open sea station, while below 60Hz, the result was reverse. It appears that a boundary layer existed between the two stations.

• Journal of Periodontal and Implant Science
• /
• v.48 no.1
• /
• pp.12-21
• /
• 2018

Purpose: The goal of this study was to develop and validate a standardized in vitro pathogenic biofilm attached onto saliva-coated surfaces. Methods: Fusobacterium nucleatum (F. nucleatum) and Porphyromonas gingivalis (P. gingivalis) strains were grown under anaerobic conditions as single species and in dual-species cultures. Initially, the bacterial biomass was evaluated at 24 and 48 hours to determine the optimal timing for the adhesion phase onto saliva-coated polystyrene surfaces. Thereafter, biofilm development was assessed over time by crystal violet staining and scanning electron microscopy. Results: The data showed no significant difference in the overall biomass after 48 hours for P. gingivalis in single- and dual-species conditions. After adhesion, P. gingivalis in single- and dual-species biofilms accumulated a substantially higher biomass after 7 days of incubation than after 3 days, but no significant difference was found between 5 and 7 days. Although the biomass of the F. nucleatum biofilm was higher at 3 days, no difference was found at 3, 5, or 7 days of incubation. Conclusions: Polystyrene substrates from well plates work as a standard surface and provide reproducible results for in vitro biofilm models. Our biofilm model could serve as a reference point for studies investigating biofilms on different surfaces.

• Journal of the Korean Society of Food Science and Nutrition
• /
• v.16 no.3
• /
• pp.21-25
• /
• 1987

In order to obtain a basic understanding of the storage of Satsuma mandarin sacs, the canned sacs were stored at room temperature from Feb. 20 to June 20(4 months), after dipping in chlorine solution and pasteurization as a pretreatment before canning. In general, the brix, acidity and pH of the sacs were almost not affected by the chlorine treatment ana a variety of pasteurization temperatures during storage. The broken rate was apperently increased by chlorine treatment, especially in the early stage of storage. However, the broken sacs were partially recovered in the end of storage. The contents of total carotenoid were almost not decreased by the conditions of the pretreatments during storage. The contents of vitamin C were sharply decreased between 3rd and 4th month of storage time on all sample. As a result, the decrement of vitamin C in canned sacs was caused by storage temperature rather than the condition of the pretreament.