• Journal of Life Science
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• v.28 no.12
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• pp.1529-1535
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• 2018

The number of antibiotic-resistant bacteria is increasing rapidly while the discovery rate of new antibiotics is in decline. A systematic study is therefore necessary to investigate which bacteria are resistant to medically important antibiotics and how high that resistance is. To that end, this study aimed to analyze which bacteria demonstrated resistance to ampicillin, one of the currently most-widely used medical antibiotics. Water samples were collected from the Changwon-Cheon that runs through Changwon City and from the pond in front of the dormitory building at Changwon University. Hundreds of ampicillin-resistant colonies were obtained and 22 morphologically distinct examples were chosen for further study. These bacteria were identified by amplifying their 16S rRNA genes and comparing those sequences with data in GenBank. The bacteria was identified as belonging to 10 families, 12 genera, and 17 species, and all were able to grow in the presence of $50{\mu}g/ml$ ampicillin while seven showed growth at ampicillin concentrations as high as 1.5 mg/ml.

• Journal of Food Hygiene and Safety
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• v.21 no.1
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• pp.23-30
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• 2006

Antimicrobial susceptibility and multiple drug resistance patterns have been carried out on total of 364 isolates of Salmonella enterica serovar Enteritidis isolated from foodborne patients in Seoul from 2001 to 2005. Overall, the highest percentage of resistance was found to the following antimicrobial agents: streptomycin (46.7%), ampicillin (37.3%), ticarcillin (36.7%), tetracycline (36.0%), nalidixic acid (20.7%), chloramphenicol (13.3%), amoxicillin/clavulanic acid (6.7%) and Ampicillin/sulbactam (4.0%). Seventy five percentage of isolates were found to be resistant to one or more of the antimicrobes tested. The resistant rates to nalidixic acid and chloramphenicol in S. Enteritidis tested were annually increased but the resistant rate to tetracycline was decreased and the resistant rates to streptomycin, ampcilin and ticarcillin were remained steadily. The most frequent patterns of multiresistant isolates were only nalidixic acid resistant (18.0%) and streptomycin-tetracycline (18.0%), streptomycin-ampicillin-ticarcillin (10%), and ampicillin-ticarcillin (5.5%). Overall the resistant rates of 1 drug was 19.3%,2 drugs 24.7%, 3 drugs 6.7% and 4 or more drugs 24.0%. The resistant rates of 1 drug and 2 drugs in 2005 were increased dramatically.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.52 no.6
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• pp.596-604
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• 2019

Vibrio parahaemolyticus causes food poisoning, mainly via marine fisheries products. We investigated the virulence factors and drug resistance of V. parahaemolyticus isolated from fisheries products purchased from the Yeosu Fisheries Market. The isolates were identified using a variety of biochemical tests and the detection of toxR and hns gene. The presence of the virulence factor-encoding genes tdh and trh in the isolates was also investigated by PCR. The resistance of the isolates to 13 antibacterial agents was tested using the disc-diffusion method and carriage of β-lactamase genes and class 1 integrons by ampicillin-resistant isolates was investigated by PCR. Four of seventeen isolates identified as V. parahaemolyticus by biochemical tests produced a species-specific PCR band. Those isolates showed >98% 16S rRNA gene sequence homology with V. parahaemolyticus and only one isolate harbored the tdh gene. All of the V. parahaemolyticus isolates were resistant to ampicillin and amoxicillin; moreover, VPA0477, a class A β-lactamase gene, and class 1 integrons were detected. Therefore, V. parahaemolyticus from fisheries products represents a low risk to human health. Also, V. parahaemolyticus is likely to develop multidrug resistance because it has class 1 integrons.

• Journal of Pharmaceutical Investigation
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• v.9 no.1
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• pp.15-19
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• 1979

The absorption rate of lysino-methylene-ampicillin from the rat small intestine, compared with ampicillin, was determined in vitro and in situ to establish the biopharmaceutical properties of lysino-methylene-ampicillin which is one of the new penicillinase-resistance antibiotics. The half of administered dose was absorbed rapidly within thirty minutes. The water-oil distribution coefficient of lysino-methylene-ampicillin was 0.03 in chloroform versus buffer system with $Na_{2}HPO_{4}-citric$ acid of pH5.2 at $37^{\circ}C$, and its dissolution rate reached the plateau in an hour and then represented 0.6 percent of equilibrium solubility

• Korean Journal of Microbiology
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• v.45 no.2
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• pp.99-104
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• 2009

This study describes the antimicrobial susceptibility and multidrug resistance patterns in 115 isolates of Salmonella enterica serovar Enteritidis from diarrhea patients in Incheon from Jan. 2004 to Dec. 2008. Overall, the highest rate of resistance was found to the following antimicrobial agents: nalidixic acid 54.8%, ampicillin 40.0%, ticarcillin 38.3%, chloramphenicol 20.9%, and tetracycline 17.4%. 91% (104 isolates) were resistant to one or more antimicrobial agents tested. Among the described antimicrobial resistant patterns, the predominant patterns were only nalidixic acid (44.3%), ampicillin-chloramphenicol-ticarcillin (11.3%,) and ampicillin-ticarcillin (10.4%). Overall, the resistant rate to one drug was 51.9%, two drugs 18.2%, three drugs 14.5%, four drugs 5.8%, and five or more drugs 9.7%.

• Korean Journal of Microbiology
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• v.49 no.2
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• pp.146-149
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• 2013

Bacteria of genus Vibrio are Gram-negative, curved, halophilic, nonspore-forming bacteria, autochthonous inhabitans of the marine and estuarine environments. Some of the Vibrio species such as V. parahaemolyticus, V. vulnificus, and V. cholerae are associated with human disease. Each year many people have been suffering from food-borne disease caused by the ingestion of seafood. In this study, we have monitored antibiotic resistance of this microorganism in 6 coastal areas of West Sea by sampling shellfish monthly. Vibrio spp. were detected from 23.3% of 120 samples analyzed using TCBS agar plates as well as API 20E kit. Among 16 antibiotics tested, resistance to vancomycin and ampicillin was observed in 82.1% of the isolates, and Vibrio spp. resistant to rifampin (71.4%) and cephalothin (53.6%) were also high. Most of the isolates were sensitive to chloramphenicol (92.9%), sulfamethoxazole/trimethoprim (92.9%), and tetracycline (96.4%). About 71.4% of the isolates showed multiple drug resistance toward 3 antibiotics including vancomycin and ampicillin.

• Journal of Food Hygiene and Safety
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• v.24 no.4
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• pp.373-377
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• 2009

From April 2008 to January 2009, a total 458 raw milk samples were randomly collected from 15 stock raising farms located in northern area of Kyunggi province and cultured for the presence of Enterococci spp. A total 170 enterococcal isolates were recovered from the raw milk samples. Enterococcus faecalis was predominant species recovered (64.7%), followed by E. faecium (18.8%), E. avium (5.9%), E. gallinarum (5.9%) and E. durans (4.7%). Antimicrobial resistance patterns of 170 Enterococci spp. against ampicillin, erythromycin, tetracyclin, chloramphenicol, vancomycin, ciprofloxacin and streptomycin were tested. According to the result, they showed high level resistance to erythromycin and streptomycin (82,9% and 93,5% respectively), moderately resistance to ampicillin, chloramphenicol and tetracyclin (50%, 45.9% and 32%, respectively) but fortunately, vancomycin and ciprofloxacin are still effective against this species.

• Korean Journal of Microbiology
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• v.42 no.4
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• pp.252-256
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• 2006

We monitored antibiotic resistance of Escherichia coli isolates from healthy volunteers of community and hospital patients from February to July in 2006. From disc diffusion test on 4915 E. coli isolates from healthy volunteers of the community, the resistance rates were as follows; tetracycline resistant, 46.6%; ampicillin resistant, 41.1%; ticarcillin resistant,37.9%. From disc diffusion test on 120 E. coli isolates from hospital patients, the resistance rates were as follows: ampicillin resistant, 66.9%; ticarcillin resistant, 63.8%; tetracycline resistant, 47.2%. Extended spectrum $\beta$-lactamase producing E. coli were isolated 0.6% and 4.1% from healthy volunteers and hospital patients.

• Korean Journal of Pharmacognosy
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• v.40 no.1
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• pp.70-76
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• 2009

Thirty six essential oils from herb drugs entered in Korean official formulary, which are frequently used in oriental region, were tested for antibiotic resistance inhibition. When the oils were combined with ampicillin (Am) or amoxicillin (Amx) they showed significant inhibitory effects on the growth of multi-drug resistant Staphylococcus aureus SA2 in considerably low concentration. The most effective combinations were oils from Acanthopanacis Cortex ($0.49{\mu}g/mL$) with Am and Cnidii Rhizoma and Lonicerae Flos (2.77 and $2.79{\mu}g/mL$, respectively) with Amx as shown in minimum resistance inhibitory concentrations.

• Korean Journal of Veterinary Service
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• v.46 no.2
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• pp.137-145
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• 2023

From November 2021 to October 2022, 151 pigs were discarded due to the suspected salmonellosis, when assessed by antemortem inspection at slaughterhouses in Gwangju. Among them, 85 cases shown with the rectal stricture were confirmed to be infected with Salmonella spp, when assessed by postmortem inspection and Salmonella-oriented culture in the laboratory, accounting for 56.3% of the carcass discard cases. Salmonella spp. were isolated in 58 cases (68.2%) out of 85 total discard cases. As for serotyping of the Salmonella isolates, 28 cases (48.3%) belonged to serogroup B and 28 cases (48.3%) belonged to serogroup C, those were the most frequent isolates, and 2 cases (3.4%) belonged to serogroup E. The most prevalent serotype was Rissen (39.7%), followed by I4,[5],12:i:-(37.9%), Derby (8.6%), Bareilly (5.2%), Infantis (3.4%), Give (3.4%), and Typhimurium (1.7%). The 58 isolates of salmonellae showed resistance to tetracycline (63.8%), chloramphenicol (56.9%), ampicillin (53.4%), and ampicillin-sulbactam (51.7%). S. Rissen showed resistance to chloramphenicol (82.6%), ampicillin (52.2%), ampicillin-sulbactam (52.2%), and tetracyclin (47.8%). S. I4,[5],12:i:-was resistant to tetracycline (90.9%), ampicillin (81.8%), ampicillin-sulbactam (77.3%), and chloramphenicol (59.1%). Among the isoates, S. Bareilly (3 strains), S. Infantis (2 strains), S. Give (2 strains), S. Rissen (3 strains), and S. I4,[5],12:i:- (1 strain) showed no resistance to the tested antimicrobials. As for the prevalence of the antibiotic resistance pattern, AM-SAM-TE-C was the most common, which was shown in 15.5% of S. Rissen. At second, the resistance pattern was occurred as AM-SAM-TE (12.1%) and AM-SAM-CF-CZ-FOX-CTX-NA-TE-C (12.1%), respectively in the case of S. I4,[5],12:i:-.