• 한국해양환경ㆍ에너지학회지
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• 제14권2호
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• pp.130-137
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• 2011

부영양화가 진행된 연안역의 퇴적환경개선을 목적으로, 수정만으로 부터 분리한 저서미세조류 Achnanthes sp., Amphora sp., Navicula sp.와 Nitzschia sp.의 성장에 미치는 수온과 염분의 영향을 정치배양으로 살펴보았다. 최대 성장속도는 Achnanthes sp.의 경우 $25^{\circ}C$와 25 psu(0.60 /day), Amphora sp.에서 $15^{\circ}C$와 25 psu(0.56 /day), Navicula sp.에서 $20^{\circ}C$와 30 psu(0.53 /day) 그리고 Nitzschia sp.에서 $20^{\circ}C$와 25 psu(0.48 /day)로 나타났다. 그리고 4종의 최적성장(최대성장 속도의 70% 이내)로부터, Amphora sp., Navicula sp.와 Nitzschia sp.는 광온성 및 광염성종으로 나타났으며, Achnanthes sp.는 낮은 수온영역에서 성장하기 어려운 협온성 종으로 특징지을 수 있었다. 따라서 Amphora sp., Navicula sp.와 Nitzschia sp.는 부영양화나 빈산소수괴 문제와 같이 오염된 연안역의 개선을 위한 식물환경복원용으로 유용한 미세조류일 것으로 판단된다.

• 한국환경과학회지
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• 제21권1호
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• pp.105-111
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• 2012

The effects of substrate size on the growth of microphytobenthos Achnanthes sp., Amphora sp., Navicula sp. and Nitzschia sp. were examined using glass beads in order for phytoremediation in the benthic layer of coastal waters. The glass beads used in this study were 0.09~0.15 mm (G.B 1), 0.25~0.50 mm (G.B 2), 0.75~1.00 mm (G.B 3) and 1.25~1.65 mm (G.B 4). No addition of glass bead used as control. The specific growth rate and maximum cell density of four microphytobenthos species were increasing with decreasing size of glass beads. Moreover, the control experiment without added attachment substrates showed the lowest specific growth rate and maximum cell density. Therefore, the suitable attachment substrates for mass culture of microphytobenthos seems to be important in order for phytoremediation using microphytobenthos.

• ALGAE
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• 제29권2호
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• pp.57-73
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• 2014

Amphora Ehrenberg ex Kutzing sensu lato is a common and widespread benthic diatom genus with a taxonomy that has been under continual revision, particularly based on molecular analyses. Although Amphora species have been studied using modern microscopy in recent years, there has not been much progress on molecular characterization of the species, especially in Asia. In this study of Amphora, sampling was carried out from September 2009 to August 2010 in Korean coastal waters. The morphological and molecular characteristics of eight Amphora sensu lato were examined: Amphora marina, A. proteus, Halamphora costata, H. coffeaeformis, H. eunotia, H. holsatica, H. terroris, and Halamphora sp. Based on previous accounts, morphology suggested that A. marina and A. proteus belong to the subgenus Amphora Cleve, which have smooth girdle bands and rather coarse and very distinct areolae on the valve. The other species, H. coffeaeformis, H. costata, H. eunotia, H. holsatica, H. terroris, and Halamphora sp. belong to the subgenus Halamphora Cleve, which was recently elevated to generic status by Levkov 2009, have plicate girdle bands, puncta which do not form straight longitudinal lines, valves which have a narrow ventral portion and apices that are generally rostrate-capitate and recurved. In agreement with analysis based on morphological characteristics, phylogenetic analysis based on small subunit rDNA suggested that the eight Amphora sensu lato species were not a monophyletic group as the morphological classification. Also, the results of molecular work and statistical analysis on all these Amphora sensu lato combined with phylogenic analysis on our geographically representative samples give strong evidence that Halamphora Levkov is independent of Amphora Cleve. Furthermore, in this study, Amphora terroris was transferred Halamphora as Halamphora terroris (Ehrenberg) Wang comb. nov. and Amphora marina was recorded for the first time in Korea.

• 한국해양학회지
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• 제26권1호
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• pp.24-37
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• 1991

저서규조류의 분포와 서식지 환경에 대한 조사가 서해에 위치한 만경-동진 펄 조 간대에서 이루어졌다. 총 60개의 정점으로부터 표층퇴적물을 정량적으로 취하여 서식 규조류의 종류를 파악하고, 개체수를 세었다. 퇴적물 입도분석을 하였으며, 정점별도 대기에 노출되는 시간의 길이로 계산하였다. 퇴적물은 외해쪽 또는 하부조간대로 갈수 록 조립해지고, 강하구쪽 또는 상부조간대로 갈수록 세립해진다. 총 371 분류군이 채 집되었으며, Navicula와 Nitzschia가 가장 우점하는 속이었다. 총 출현 개체수에서 1% 이상을 차지하는 규조는 다음6종이다. Paralia sulcata, Navicula sp. 1, Navicula arenaria, Cymatosira belgica, Amplora holsatica, Amphora coffeaeformis, Achnanthes hauckiana, Rhaphoneis amphiceros, Thalassionema nitzschioides. Navicula sp. 2, Dimeregramma minor, Amphora sp. 1, Cyclotella atomus, C, striata, Nitzschia kuetzingiana, Stephanodiscus sp. 1.이들 16종의 조사정점에 따 른 개체수 분포를 기재하였다. 우점종의 대부분은 니질성 규조이며, 그 분포범위 는 매우 넓다. 사질성규조인 무배선, 단배선형에 속하는 종들은 하부조간대에 국한되 어 분포한다. 사질성규조로서 대표적인 종류는 다음의 것들이다. Cocconeis sp. 1, Opephora martyi, Amphora sabyii, Dimeregramma minor var. nana, Fragilaria virescens var. oblongella, F. virescens, Cocconeis grata. 일정 면적당 개체수는 세립질 퇴적물이 우세한 상부조간대와 강하구에서 많다.

• Preventive Nutrition and Food Science
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• 제13권3호
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• pp.166-175
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• 2008

In this study, we focused on natural water-soluble antioxidants from the Jeju benthic diatoms, Achnanthes longipes, Navicula sp. and Amphora coffeaeformis. They were prepared by enzymatic digestion using five carbohydrases (Viscozyme, Celluclast, AMG, Termamyl and Ultraflo) and five proteases (Protamex, Kojizyme, Neutrase, Flavourzyme and Alcalase) and their potential antioxidant activity was assessed. Among the enzymatic digests, Neutrase digest from A. coffeaeformis exhibited the highest effect in DPPH radical scavenging. Flavourzyme (48.7%), Viscozyme (47.4%) and Celluclast (45.7%) digests from Navicula sp. exhibited higher $O^{{\cdot}-}_2$ radical scavenging activity. Viscozyme digest from A. coffeaeformis (45.9%) possessed the highest effects in hydroxyl radical scavenging. Termamyl (89.3%) and Protamex (88.8%) digests from A. coffeaeformis had strong metal chelating activity. Lipid peroxidation was significantly inhibited in Termamyl and Kojizyme digests from A. longipes, AMG and Termamyl digests from Navicula sp. and Kojizyme digest from A. coffeaeformisi. These data suggest that enzymatic digests of the Jeju benthic diatoms might be valuable sources of antioxidant which can be applied in food and pharmaceutical industry.

• ALGAE
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• 제24권1호
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• pp.47-55
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• 2009

Recently, interest in plant-derived food additives has developed natural antioxidants, in order to alternate syn-thetic antioxidants with several disadvantages. In the present study, different organic fractions from solvent parti-tions of 80% methanol extract from Jeju benthic diatoms, Achnanthes longipes, Navicula sp. and Amphora coffeaeformis was assessed for their potential antioxidant effects. Among the solvent fractions tested, n-hexane (80.4%) and 80% methanol extract (76.6%) from A. longipes, chloroform (63.2%) from Navicula sp. and n-hexane (67.4%) from A. cof-feaeformis were effective in DPPH free radical scavenging. Fractions of chloroform (53.4%) and n-hexane (53.1%) from A. longipes exhibited higher activities on $H_2O_2$ scavengin. Fraction of n-Hexane from A. longipes exhibited the highest hydroxyl radical scavenging activity and NO. scavenging activity (56.5% and 75.6%, respectively). Aqueous residue from A. coffeaeformis (75.6%) showed the highest metal chelating effect. chloroform and ethyl acetate frac-tion of all the diatoms exhibited significant antioxidant activities in lipid peroxidation inhibitory activity. In particu-lar, both chloroform and the ethyl ecetate fraction from A. longipes and A. coffeaeformis exhibited lipid peroxidation inhibitory activity significantly higher than that of $\alpha$- tocopherol. These data suggest that the Jeju benthic diatoms tested are rich in hydrophilic and hydrophobic antioxidative compounds with different antioxidative properties that can be applied in food industry.

• 한국환경생태학회지
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• 제29권6호
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• pp.884-894
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• 2015

한국 태안군 정산포와 황도 갯벌에서 저서미세조류의 종조성과 생물량의 계절적 변화가 바지락 생장과 연관성이 있는지를 파악하기 위하여 2012년 2월부터 11월까지 계절별로 조사되었다. 연구기간 동안 저서미세조류는 총 122종이 관찰되었으며, 정산포에서 85종(계절별 30-45종), 황도에서는 92종(32-57종)이었다. 연평균 엽록소 a의 농도는 정산포에서 $79.75mg/m^2$였고 황도에서 $151.50mg/m^2$였다. 갯벌 퇴적물에서 서식하는 저서미세조류의 연평균 세포수는 정산포에서 $13,255cells/cm^2$이었고 황도에서 $15,943cells/cm^2$였다. 정산포에서 Cylindrotheca closterium, Gyrosigma sp.와 Navicula sp.가 우점하였고 황도에서는 Amphora sp.1., C. closterium, Detonula pumila, Navicula sp.와 Merismopedia sp.였다. 또한, 두 연구정점에서 최대의 세포수를 나타낸 종은 Paralia sulcata였다. 정산포 바지락의 소화기관에서는 계절별로 18-31종의 미세조류가 분류되었으며, 황도 바지락 소화기관에서는 19-25종의 미세조류가 동정되었고 P. sulcata는 모든 계절에 바지락 소화기관에서 확인되었다. 바지락의 연평균 비만도는 정산포에서 연평균 0.42로 황도의 0.57에 비해 0.15가 낮았다. 결과적으로 바지락의 먹이원인 저서미세조류의 풍도와 엽록소 a의 농도는 바지락 생장과 밀접한 관련이 있음을 확인하였다.

• 한국환경과학회지
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• 제12권7호
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• pp.715-724
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• 2003

Distributional patterns of microalgae were studied in the tidal flats of Gamami Beach(Young-Gwang, Korea) from November 1999 to 2000 July. The tidal flats of Gamami Beach was composed mainly of sandy sediment. The concentrations of nutrients were low compared with other tidal flats. In the present study, 68 species of microalgal flora were identified. These were comprised of 25 species of benthic microalgae and 59 species of planktonic microalgae. Diatoms predominated the benthic microalgae with 96.0% of total species occurred. Dominant species were Amphora sp., Cocconeis sp., Coscinodiscus asteromphalus, Coscinodiscus sp., Nitzschia sigma var. intermedia, Nitzschia distans, Navicula spp., Paralia sulcata, Pleurosigma sp. Skeletonema coastatum, and Surirella sp. Among them, Amphora sp., Paralia sulcata, and species of Pleurosigma and Nitzschia were observed throughout the studied period. Planktonic microalgae of Gamami Beach was also predominated by diatoms. They occupied 88.1% of total planktonic microalgae. The density of microalgal population was higher in silty sediment than in sandy sediment. The population density of microalgae was higher in high tide zone than that in low tide zone. The density of the benthic microalgae in the surface layer of tidal flats showed increasing tendency for 2 hours after the beginning of ebb tide. On the contrary, benthic microalgal density of subsurface layer was decreased during the period. Concentrations of chlorophyll ${\alpha}$ from sediment and water were not synchronized during the study period. Therefore, the distributional patterns of the benthic microalgae and planktonic algae seemed not to be related. Chlorophyll ${\alpha}$ of water was highly related with the concentration of NH$_4$-N, whereas, chlorophyll ${\alpha}$ of sediment uas related with NO$_3$-N concentration.

• ALGAE
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• 제17권2호
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• pp.117-125
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• 2002

Five microalgal species isolated from the Jeju coast and four microalgal stock strains in hatchery were cultured in order to investigate their adapation to extreme changes in environmental factors such as salinity, water temperatue, adn nutrients. In case of salinity variation, Nitzschia sp. of Bacillariophyceae, Isochrysis galbana of Haptophyceae and Tetraselmis gracilis of Prasinophyceae showed optimum growth at the low salinity of 20 and 25 psu. Amphora coffeaeformis and Chetoceros simplex of Bacillariophyceae, and Pavlova lutheri of Haptophyceae adapted well at the relatively high salinities of 30 and 35 psu. However Phaeodactylum tricornutum of Bacillariophyceae and Chlorella sp. of Chlorophyceae showed euryhaline property In case of water temperature variation, most of all the species studied wer inhibited at 10℃. C. simplex, Nitzschia sp., p. tricornutum, Chlorella sp. and T. gracilis grew well at above 20℃. A. coffeaeformis, I. galbana and P. lutheri adapted also at the high temperature of 30℃. Each microalgal strain showed different growth rates and its maximum biomass. Generally microalgal populations from the Jeju coast grow well in relatively high salinity and high water temperature. Their growth were inhibited at low water temperature, but not likely affected at low salinity. This study indicates that the microalgal populations could not be affected by abnormally low salinity phenomena, which have happened occasionally around the west Jeju coast in summer and have led macrobenthic animals to mass mortality.

• 한국패류학회지
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• 제29권3호
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• pp.197-205
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• 2013

Even though seedling production of Haliotis discus hannai has fully developed, the culture of benthic diatom as a live food for larvae is still a barrier to solve in commercial hatchery. The farmer depends on mixed microalgae which are non-selectively attached on the plate by flowing of natural seawater. The adequate diatom on the plate for the larvae in terms of quality and quantity is always significant bottleneck in the hatchery. In this study, two benthic diatom species, Caloneis schroederi and Rhaphoneis sp. were separately cultured in mass and inoculated to four tons' settlement tank of the larvae. And the larvae and the spats were cultured for nineteen and nine weeks, respectively. The result on seedling production of H. discus hannai with this method was compared to that of the farmer's traditional method as a control. With regard to variation of species composition of benthic diatom on the plate, C. schroederi and Rhaphoneis sp. were dominant for first three weeks after inoculation. But the diverse diatoms mainly, Navicula, Amphora, Cylindrotheca, Licmophora, Pleurosigma began to attache on the plate from the 4th week. The larvae attached 2.5 times more in C. schroederi tank than in the control tank. The final total biomass of the seeds in Rhaphoneis sp. tank was 3.2 times more than that of the control tank. The retared-spats in the seedling production also showed significantly higher growth and survival in the spat fed Rhaphoneis sp. or C. schroederi than those in the control group. We suggest that C. schroederi is proper for settlement of the larvae and Rhaphoneis sp. is appropriate for the growth of the larvae and spats in the commercial hatchery of H. discus hannai.