• 제목/요약/키워드: Ampelopsis japonica

검색결과 6건 처리시간 0.019초

백렴 추출물(抽出物)이 종양면역(腫瘍免疫)에 미치는 영향 (Effects of Ampelopsis japonica Extracts on Tumor Immunity)

  • 박승만;안상우;조종관
    • 한국한의학연구원논문집
    • /
    • 제11권2호
    • /
    • pp.113-140
    • /
    • 2005
  • 1. 백렴 추출물은 부분적으로 종양세포인 HT1080, Hep G2, MCF-7 cell에 대해 세포독성을 보였으나, 섬유세포인 7250 cells과 종양세포인 CT-26 cells에서는 세포독성은 나타나지 않았다. 2. 백렴 추출물은 macrophage에서 생산되는 NO production을 부분적으로 증가시켰다. 3. 백렴 추출물은 macrophage 활성화와 관련 있는 IL-1과 iNOS의 유전자 발현을 증가시켰다. 4. 백렴 추출물은 NK cell의 cytotoxicity를 활성화시켰다. 5. 백렴 추출물은 NK cell 활성화와 관련있는 IL-1, IL-4, IL-10, IL-12, iNOS, IFN-${\gamma}$, TNF-${\alpha}$ 의 유전자 발현을 증가시켰다. 6. 백렴 추출물은 마우스의 비장세포에서 IL-10, IFN-${\gamma}$, TNF-${\alpha}$ 의 단백질 발현을 증가시켰다. 7. 백렴 추출물은 CT-26 cell에 의한 pulmonary colony를 대조군에 비해 유의성 있게 억제시켰다. 이상의 결과로 백렴 추출물은 macrophage와 NK cell의 활성화를 통해 면역 조절과 항암제로서의 유용성이 기대된다.

  • PDF

백렴(白蘞)의 파골세포 분화 및 관련 유전자 발현 억제에 미치는 영향 (The Effect of Ampelopsis japonica (Thunb.) Makino on Osteoclastogenesis and Expression of Osteoclast-Related Gene)

  • 김홍식;이수민;김민선;김재현;강예진;권성준;남영우;유승우;최홍석;허선진;손영주;정혁상
    • 대한본초학회지
    • /
    • 제38권5호
    • /
    • pp.9-19
    • /
    • 2023
  • Objectives : Osteoporosis is a systemic skeletal disorder characterized by reduced bone mineral density and increased risk of fractures. Bisphosphonates and selective estrogen receptors, which are bone resorption inhibitors that are currently widely used as osteoporosis treatments, show serious side effects when administered for a long time. Research on bone resorption inhibitors that complement the problems of existing treatments is needed. The purpose of this study was to investigate the effect of inhibiting osteoclast differentiation and activity on the tuberous root of Ampelopsis japonica (Thunb.) Makino (AM). Methods : After extracting AM using distilled water and ethanol, the inhibitory effects of the two solvents on osteoclast differentiation were compared using the RANKL-induced in vitro experimental model and the TRAP assay kit. The impact of AM on bone resorption was investigated through the pit formation assay, and its effect on F-actin formation was assessed through fluorescent staining. Additionally, protein and mRNA expression levels of osteoclast differentiation markers (NFATc1, c-Fos, TRAP and ATP6v0d2) and resorption markers (MMP-9, CTK, and CA2) were analyzed via western blot and RT-PCR. Results : AM treatment significantly decreased the number of TRAP-positive cells and pit formation area. Furthermore, AM suppressed both the protein and mRNA expression of NFATc1 and c-Fos, key transcription factors involved in osteoclast differentiation and it downregulated the expression of osteoclast-associated genes such as TRAP, CTK, MMP-9, CA2, and ATP6v0d2. Conclusions : These results suggest that AM can inhibit bone resorption and osteoclast differentiation, indicating its potential for use in the treatment and prevention of osteoporosis.

한국 약용식물 추출물의 알도즈 환원 효소 억제 효능 검색(IX) (Screening of Korean Herbal Medicines with Inhibitory Effect on Aldose Reductase (IX))

  • 최소진;김영숙;김주환;김진숙
    • 생약학회지
    • /
    • 제45권4호
    • /
    • pp.354-358
    • /
    • 2014
  • Aldose reductase (AR) has been demonstrated to play important role in the development of the diabetic complications such as diabetic retinopathy, diabetic neuropathy and diabetic nephropathy. To discover novel treatments for diabetic complications from natural sources, 69 Korean herbal medicines have been investigated for inhibitory activities on AR. Among them, 7 herbal medicines, Eleutherococcus sessiliflorus (stems), Artemisia japonica (whole plants), Wisteria floribunda (leaves), Eurya japonica (stems, twigs and leaves, leaves), Ampelopsis brevipedunculata (stems) exhibited a significant inhibitory activity compared with 3,3-tetramethyleneglutaric acid as positive control.

도시지역 곰솔림의 식생복원모델 (Vegetation Restoration Model of Pinus thunbergii in Urban Areas)

  • 김석규
    • 환경영향평가
    • /
    • 제20권2호
    • /
    • pp.151-162
    • /
    • 2011
  • The purpose of this study is suggest to restoration model of Pinus thunbergii in Saha-gu, Busan Metropolitan City. The result of this study is summarized as follows; As the results of this study, vegetation restoration model is presented by separating community planting and edge planting. The community planting species of tree layer were Pinus thunbergii and Quercus acutissima and Quercus dentata and Quercus serrata and Quercus alienna and Quercus variabilis. The community planting species of subtree layer were Platycarya strobilacea and Prunus sargentii and Styrax japonica and Eurya japonica and Morus bombycis. The community planting species of shrub layer are Ulmus pavifolia and Ulmus davidiana and Lindera obtusiloba and Elaeagnus macrophylla and Mallotus japonicus and Ligustrum obtusifolium and Sorbus alnifolia and Rhus trichocarpa and Zanthoxylum schinifolium and Rosa wichuraiana and Rhus chinensis and Viburnum erosum and Rhododendron mucronulatum and Rhododendron yedoense and Indigofera pseudotinctoria. And the planting species of edge vegetation are Japanese Angelica and Symplocos chinensis and Pittosporum tobira and Lespedeza maximowiczii and Lespedeza bicolor and Rubus coreanus and Rubus idaeus and Vitis thunbergii and Ampelopsis brevipedunculata and Rosa multiflora. Considering the population of individuals up to layers in each $400m^2$ area, it was composed of 24 in tree layer, 35 in subtree layer, 410 in shrub layer and 34% herb layer in the Pinus thunbergii community. And the average of breast-high area and canopy area was $10,852cm^2$ in tree layer, in subtree layer $1,546cm^2$, in shrub layer $1,158,660cm^2$. The shortest distance between trees was calculated as 2.0m in tree layer, 1.9m in subtree layer.

도시림 식생의 생태적 특성과 복원모델 (Ecological Characteristics and Restoration Model of Vegetation in the Urban Forest)

  • 김석규;주경중;남정칠;박승범
    • 한국환경복원기술학회지
    • /
    • 제13권2호
    • /
    • pp.80-94
    • /
    • 2010
  • The purpose of this study is suggest to restoration model of Pinus thunbergii in Saha-gu, Busan. The result of this study is summarized as follows. As the results of this study, vegetation restoration model is presented by separating community planting and edge planting. In community planting, as a group of canopy, there are 6 species; Pinus thunbergii, Quercus acutissima, Quercus dentata, Quercus serrata, Quercus alienna, Quercus variabilis. As a group of understory, there are 5 species; Platycarya strobilacea, Prunus sargentii, Styrax japonica, Eurya japonica, Morus bombycis. Also as a group of shrub, there were 15 kinds of species; Ulmus pavifolia, Ulmus davidiana, Lindera obtusiloba, Elaeagnus macrophylla, Mallotus japonicus, Ligustrum obtusifolium, Sorbus alnifolia, Rhus trichocarpa, Zanthoxylum schinifolium, Rosa wichuraiana, Rhus chinensis, Viburnum erosum, Rhododendron mucronulatum, Rhododendron yedoense, Indigofera pseudotinctoria. And as a group of edge vegetation, there were 10 kinds of species; Japanese Angelica, Symplocos chinensis, Pittosporum tobira, Lespedeza maximowiczii, Lespedeza bicolor, Rubus coreanus, Rubus idaeus, Vitis thunbergii, Ampelopsis brevipedunculata, Rosa multiflora. Vegetation restoration models of Pinus thunbergii community were calculated the units $400m^2$ for the average populations of the woody layer is 24 in canopy layer, 35 in understory layer, 410 in shrub layer, 34% herbaceous layer ground cover. And the average of breast-high area and canopy area is $10,852cm^2$ in canopy layer, in understory layer $1,546cm^2$, in shrub layer $1,158,660cm^2$. The shortest distance between trees is calculated as 2.0m in canopy layer, 1.9m in understory layer.

제주 정의읍성 벽면식생에 근거한 벽면식재용 식물 선정에 관한 기초연구 (A Basic Study on the Selection of Plants for the Walls of Fortresses in the Case of the Jeongyi Town Wall in Jeju)

  • 노재현;오현경;최영현;김영숙
    • 한국조경학회지
    • /
    • 제42권2호
    • /
    • pp.33-40
    • /
    • 2014
  • 본 연구는 제주도를 비롯하여 온대남부지방 성벽 벽면녹화에 적절한 식물종 선정을 위한 기초자료 제시를 목적으로 시도되었다. 제주 정의읍성 외곽 성벽의 식생을 조사 분석하여, 그 벽면식생의 가치특성과 문제점을 진단한 본 연구의 주요 결론은 다음과 같다. 성벽에 자생하는 식물은 총 52분류군이며, 이 중 방가지똥, 약모밀, 주홍서나물, 개망초, 자주광대나물 등 5분류군은 귀화식물로 분류되었다. 한편, 조사구별 출현종수는 3~14분류군이며, 평균 자생식물은 7.1분류군으로 나타났다. 상재도 III등급 이상의 식물은 말똥비름, 하늘타리, 송악, 모시풀이었으며, 종조성표 조작 결과, 정의읍성 성벽에 자생하는 식물종은 군락구분종 11분류군, 수반종 41분류군으로 총 52분류군으로 밝혀졌다. 자료 고찰 결과, 성벽 벽면식재를 위해 활용성이 높은 식물은 송악, 왕모람, 줄사철나무 등의 상록성 만경식물과 담쟁이덩굴, 하늘타리, 계요등 등의 낙엽성 만경식물로 밝혀졌다. 특히 왕모람은 남향의 성벽에 더욱 유용한 법면식생 재료로 추천한다. 이밖에 으름덩굴, 푼지나무, 가새잎개머루, 장딸기, 사위질빵 등의 목본식물과 각시마, 단풍마, 부채마, 거지덩굴 그리고 좁은잎계요등 등의 초본식물은 남부지방의 성벽을 비롯한 벽면식재용 재료로 활용 가능성이 높은 것으로 판단된다. 이밖에 콩짜개덩굴을 비롯하여 봉의꼬리, 도깨비 쇠고비, 실고사리 등의 양치식물은 제주도를 비롯하여 남부지방 성벽 고유의 지역성 반영을 위해 매우 유용한 식물로 판단된다.