• 한국균학회지
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• 18권2호
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• pp.109-114
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• 1990

The effect of different carbon and nitrogen compounds on the growth and sporula­tion of Curvularia clavata Alcorn (Herberium No. IMI264075) has been studied. All the carbon sources tried were well utilized by the pathogen though glucose, and sucrose supported the best growth while glucose, maltose and sucrose the sporulation of the fungus. Of the nine nitrogen compounds, L-glutamic acid supported the best growth while aspartic acid and L-glutamic acid the sporulation of the fungus. Growth and sporulation were generally better with organic than inorganic nitrogen sources. Ammonium sulphate was the best inorganic source. A sudden drop of pH value of the culture media after 4 days of incubation did not favour good growth of the fungus.

• 자원리싸이클링
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• 제2권4호
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• pp.23-26
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• 1993

This work is carried out to develop the recovery process of vanadium as vanadium pentoxide and nickel as nickel sulphate from the leaching solution of heavy oil fly ash. First, sodium chlorate solution was added to the leaching solution to oxidize vanadium ions. With adjusting pH of the solution and heating, vanadium ions(V) is hydrated and precipitated as red cake of $V_2O_5$ from the solution. After recovering vanadium, nickel is recovered as ammonium nickel sulfate with crystallization process. From this nickel salt, nickel sulfate which meets the specifications for the electroplating industry can be produced economically. More than 85% of vana-dium and nickel in the fly ash are recovered in this process.

• 한국결정성장학회지
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• 제2권1호
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• pp.60-69
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• 1992

$TiCl_4$와(NH_4)_2SO_4$를 액상 반응시켜 암모늄 티타늄 설페이트를 형성시킨 뒤 하소하여 미립의 $TiO_2$분말을 만들었다. Anatase 분말로 Verneuil장치에서 Rutile단결정을 성장시킨 뒤 물성을 조사하였다. 최적의 성장조건은 $H_2:O_2=3:1$ 성장온도는 $1900^{\circ}C$ 분말공급속도는 10g/hr였다.

• 대한미생물학회지
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• 제18권1호
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• pp.67-71
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• 1983

Antisera to ox red blood cells were prepared by injection of ox red blood cell stroma without adjuvant in outbred white rabbits. Purified IgM fraction for T-cell subset assay was obtained from these rabbit anti-ox red blood cell stroma antisera by precipitation with 50% saturated ammonium sulphate followed by DEAE-Cellulose chromatography and Sephadex G-200 gel filtration. The serological identification of purified IgM fraction was achieved by immunoelectrophoresis with guinea pig antiserum against rabbit anti-ox red blood cell IgM antibody.

• Advances in nano research
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• 제3권2호
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• pp.67-79
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• 2015

Air stable nanoparticles were prepared from cobalt sulphate using tetra butyl ammonium bromide as surfactant and sodium borohydride as reductant at room temperature. The cobalt nanocolloids in aqueous medium were found to be efficient catalysts for the degradation of toxic organic dyes. Our present study involves degradation of Methylene Blue and Rhodamine-B using cobalt nanoparticles and easy recovery of the catalyst from the system. The recovered nanoparticles could be recycled several times without loss of catalytic activity. Palladium nanoparticles prepared from palladium chloride and the same surfactant were found to degrade the organic dyes effectively but lose their catalytic activity after recovery. The cause of dye colour discharge by nanocolloids has been assigned based on our experimental findings.

• 폴리머
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• 제34권4호
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• pp.306-312
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• 2010

Triphenylvinylsilane (TPVS) containing vinyl acetate (VAc), butyl acrylate (BA), and Nmethylolacrylamide (NMA) copolymers were prepared by emulsion polymerization. The polymerization was performed at $80^{\circ}C$ in the presence of auxiliary agents and ammonium peroxodisulfate (APS) as the initiator. Sodium dodecyl sulphate (SDS) and Arkupal N-300 were used as anionic and nonionic emulsifiers, respectively. The resulting copolymers were characterized by using Fourier transform infrared spectroscopy (FTIR), gel permeation chromatography (GPC), and dynamic light scattering (DLS). Thermal properties of the copolymers were studied by using thermogravimetric analysis (TGA) and differential scanning calorimetry (DSC). The morphology of copolymers was also investigated by scanning electron microscopy (SEM) and then the effects of silicone concentrations on the properties of the TPVS-containing VAc-acrylic emulsion copolymers were discussed. The obtained copolymers have high solid content (50%) and can be used in weather resistant emulsion paints as a binder.

• Archives of Pharmacal Research
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• 제13권1호
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• pp.28-32
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• 1990

Precipitation and purification of amylase secreted by Streptomyces aureofaciens 77 in liquid inorganic salts-starch medium under the optimum conditions were carried out. Ammonium sulphate fractionation was used to precipitate amylase in cell free culture filtrate. (NH/sub 4/)/sub 2/ SO/sub 4/ at a concentration of 50-70% saturation gave the highest enzyme yield. The obtained precipitates were redissolved in phosphate buffer (pH 7.0) and subjected to dialysis. The dialyzed enzyme preparation was applied to DEAE-cellulose column chromatography which resulted in an increase of purification up to 59.48 fold. A further step of purification was done by applying the obtained purified sample to Sephadex-G200 column chromatography which resulted in ann increase of purification up to 73. 92 fold. The results clearly indicated that the isolated amylase from S. aureofaciens 77 was only on type.

• Preventive Nutrition and Food Science
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• 제9권3호
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• pp.283-288
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• 2004

Superoxide dismutase (SOD) from tomato (Lycopersicon esculentum Mill.) fruit was purified by ammonium sulphate precipitation, Sephadex G-100 and DEAE-cellulose column chromatographies. A 22 fold purification and an overall yield of 44% were achieved. The purified enzyme was a homodimer with Mr 37.1 kDa and subunit Mr 18.2 kDa as judged by SDS-PAGE. SOD showed $K_{m}$ values of 25 ${\times}$ 10$^{-6}$ M and 1.7 ${\times}$ 10$^{-6}$ M for nitroblue tetrazolium (NBT) and riboflavin as substrates, respectively. The enzyme was thermostable upto 5$0^{\circ}C$ and exhibited pH optima of 7.8. The effect of metal ions and some other compounds on enzyme activity was studied. $Co^{2+}$ and $Mg^{2+}$ were found to enhance relative enzyme activities by 27 % and 73 %, respectively, while M $n^{2+}$ inhibited the SOD activity by 64%. However, $Ca^{2+}$ and C $u^{2+}$ had no effect on enzyme activity. Other compounds like $H_2O$$_2$ and Na $N_3$ inhibited enzymatic activities by 60% and 32%, respectively, while sodium dodecyl sulphate (SDS), chloroform plus ethanol and $\beta$-mercaptoethanol had no effect on the activity of SOD. of SOD.

• Journal of Dairy Science and Biotechnology
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• 제21권2호
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• pp.114-119
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• 2003

Lactobacillus acidophilus CP4A 균주가 생산하는 acidocin 4A를 정제하고자 ammonium sulphate 침전법 , Octyl-sepharose CL-4B column chromatography, $C_{18}$ Sep-Pak cartridge, $C_{18}$ RP HPLC, HPLC gel filtration을 실시하였고 tricine SDS-PAGE를 통해 약 4.1 kDa의 박테리오신임을 확인하였다. Acidocin 4A의 항균작용 기작을 L. delbrueckii subsp. lactis ATCC 4797을 대상으로 TEM을 이용해 관찰한 결과 세포벽이 해리되고 세포벽사이로 세포내용물이 용출되어 궁극적으로 cell lysis가 일어나는 bacteriolytic 현상을 확인하였다. 또한, acidocin 4A의 생산에 관련된 유전자의 존재 위치를 파악하고자 EtBr을 이용한 curing방법을 실시하였으며 그 결과 약 20 kb 크기의 plasmid에 acidocin 4A 생산과 자체 면역에 관련된 유전자가 존재함을 알 수 있었다.

• 대한미생물학회지
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• 제15권1호
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• pp.71-75
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• 1980

사람에 있어서 면역(免疫)담당세포의 하나인 T세포(細胞)는 몇몇 subpoulation으로 나누어지고 있으며 그중 $T_M$ 및 $T_G$를 동정(同定)하는 수단으로 사용되는 우적혈구항체(牛赤血球抗體)중에서 우선 순수(純粹) IgG항체(抗體)를 분리(分離) 정제(精製)하였으며, 이 정제(精製)된 IgG항체(抗體)는 표준제품(標準製品)과의 비교실험(比較實驗)에서 $T_G$세포(細胞)의 일치(一致)되는 성적(成績)을 얻을 수 있었다.