• 한국산업융합학회 논문집
• /
• 제6권2호
• /
• pp.123-129
• /
• 2003

Oxindoles(6a-g) are obtained by rhodium(II)-catalyzed Wolff rearrangement of diazoquinolinediones in moderate yields. Treatment of 6a-g oxindole derivatives reacted with CAN in acetonitrile at room temperature to gave 7a-g isatins in good yields. The structures of these compound were identified by MP, IR and NMR-spectra.

• 대한약학회:학술대회논문집
• /
• 대한약학회 2003년도 Proceedings of the Convention of the Pharmaceutical Society of Korea Vol.1
• /
• pp.283.2-283.2
• /
• 2003

This study established a highly sensitive novel quantification method for detecting felodipine in human plasma using LC-MS/MS. The mobile phase used after degassing was composed of 1 mM ammonium acetate and acetonitrile (20:80, pH 6.0), with flow rate of 200 uL/min. One mL plasma were pipetted into glass tubes and spiked with 0.1 mL of internal standard solution. (omitted)

• 대한약학회:학술대회논문집
• /
• 대한약학회 2003년도 Proceedings of the Convention of the Pharmaceutical Society of Korea Vol.1
• /
• pp.282.1-282.1
• /
• 2003

This study established a sensitive novel Quantification method for detecting glimepiride in human plasma using LC-MS/MS for pharmacokinetic studies. The mobile phase used after degassing was composed of 10 mM ammonium acetate and acetonitrile (20:80, pH 3.0), with flow rate of 200uL/min. One mL plasma were pipetted into glass tubes and spiked with 0.1 mL of internal standard solution. (omitted)

• 한국산업보건학회지
• /
• 제4권2호
• /
• pp.137-147
• /
• 1994

This study was designed to identify and quantitate airborne isocyanate simultaneously by HPLC. These samples were collected using 1-(2-pyridyl)piperazine(1-2PP) coated glass fiber filter from polyurethane painting works at 8 wood furniture factories in Kimpo and Inchun. The results obtained were as follows : 1. The most suitable mobile phase condition of simultaneously analyzing isocyanates was 0.01 M ammonium acetate buffer ACN(70/30) adjusted to pH 6.2 from the beginning of the analysis to 20 min and 0.01 M ammonium acetate buffer/ACN(50/50) adjusted to pH 6.2 from 21 min to 40 min using the gradient mode. The peaks of isocyanates were able to obtain within 30 min. 2. The recovery efficiencies for 2,6-TDI, 2,4-TDI, HDI and MDI urea derivates spiked at the target concentration on coated glass fiber tillers were 91.00, 93.42, 91.31 and 94.21 %, respectively. 3. The qualitative analysis of the isocyanates samples from polyurethane spray painting works in wood furnture factories identified Ihree isocyanates, 2,6-TDI, 2,4-TDI and MOI. And their concentration ranges were 0-312.6, 0-56.3 and $0-62.1{\mu}g/m^3$, respectively. A disadvantage of using the colorimetric method for isocyanate analysis is its inability of separating isocyanates. This study identified such three isocyanates as 2,6-TDI, 2,4-TDI and MDI from polyurethane spray painting works in wood furniture factories. These isocyanates were successfully quantitated by HPLC by modifying the mobile phase condition and switching to gradient mode.

• 한국미생물·생명공학회지
• /
• 제33권2호
• /
• pp.112-116
• /
• 2005

스웨덴 산림지역의 토양시료로부터 분리한 Bacillus licheniformis KMU-3의 작물병원성 곰팡이에 대한 항진균 활성 및 항진균물질의 생산조건에 대하여 검토했다. B. licheniformis KMU-3은 Rhizoctonia solani KACC 40101에 대하여 높은 항진균활성을 나타냈었다. 항진균물질 생산을 위한 최적배양조건은 탄소원으로 $1.0\%$ sodium acetate, 질소원으로는 $1.0\%$ ammonium sulfate, $24^{\circ}C$, pH 8.0, 180 rpm, 24시간 진탕배양하였을 때 가장 높은 항진균활성를 나타내었다. TLC상에서 Rf값이 0.49인 밴드에 나타난 물질에서 항진균활성이 확인되었으며, 이물질은 Rhizoctonia solani AG-4 KACC 40142, Colletotrichum orbiculare KACC 40808, Colletotrichum gloeosporioides KACC 40804, Cladosporium cucumerinum KACC 40576, Fusarium graminearum KACC 41040, Fusarium oxysporum KACC 40052에 대하여서도 높은 항진균활성을 나타내었다.

• 대한화학회지
• /
• 제53권3호
• /
• pp.308-324
• /
• 2009

현재 수행하는 작업은 피페리딘 또는 암모늄 아세테이트 존재하에서 malononitrile와 $\beta$- aroylacrylic acid 유도체의 DMF 용매조건에서 상호작용에 대한 연구이며, 형성된 화합물을 이용한 퓨즈 되고 단리된 이중고리화 시스템의 합성에 관한 것이다. $\beta$-aroylacrylic acid (3)이 DMF 용매와 피페리딘 촉매조건에malononitrile와 반응하여 4H-피란유도체(4)를 형성한다. 촉매를 암모늄아세테이트로 바꿈으로서 피리딘 유도체를 얻었다. 또한 N-말레암산 유도체 (19)와 (27)은 말레 무수물과 함께 (4)와 (5)의 반응을 경유하여 합성되었다. 마이클 첨가 반응에서 이용되는 메틸렌화합물에 관한 이 연구는 B-aroylacrylic acid의 경우와 유사하게 형성된 말레암산 유도체의 반응성에 대한 것이다.

• 한국미생물·생명공학회지
• /
• 제30권1호
• /
• pp.63-67
• /
• 2002

본 연구에서 사용된 Bifidobacterium은 모유를 섭취한 유아의 분변으로부터 분리었으며 16s rRNA 염기서열분석 결과 Bifidobacterium longum속으로 동정되어 Bifidobacterium SH2라 명명하였다. Bifidobacterium SH2의 고농도 배양을 위하여 기본 배지로 MRS 배지를 변형하여 최적화한 결과 최적 배지의 조성은 50g/L lactose, 10 g/L beef extract, 10 g/L peptone, 5 g/L yeast extract, 7 g/L sodium acetate, 2 g/L ammonium citrate, 2 g/L disodium phosphat, 1 g/L tween 80, 0.2 g/L $MnSO_4$, 0.5 g/L L-cysteine이었다. 배양조건을 최적화한 결과 pH는 5.0에서, 온도는$ 37^{\circ}C$에서 균체량이 최대로 나타났다. 배지와 배양 조건을 최적화하여 건조 균체량과 생균수가 MRS 배지 보다 각각 2.7배, 1.8배 증가한 결과를 얻었다.

• 방사선산업학회지
• /
• 제9권2호
• /
• pp.63-68
• /
• 2015

Lyophilized DOTMP kits were prepared using DOTMP, ammonium acetate, and ascorbic acid. The $^{68}Ga$-DOTMP was prepared by incubating the kit dissolved in 0.5 ml of concentrated $^{68}Ga$ using NaCl method and 0.5 ml of DDW, at $100^{\circ}C$ for 7 min. The labeling yield was evaluated by two solvent systems of TLC. 1 MBq of concentrated $^{68}Ga$ was labeled with $0.8{\mu}g$ of DOTMP by high radiolabeling yield (>98%), which was determined by two TLC methods. The composition of the prepared freeze-dried vial is $400{\mu}g$ of DOTMP, 19.27 mg of ammonium acetate and 17.62 mg of ascorbic acid. ~555 MBq of $^{68}Ga$-DOTMP was prepared with excellent radiochemical purity (>98%) and it was stable for 4 hr at room temperature. In conclusion, Freeze-dried DOTMP kits for the convenient preparation of $^{68}Ga$-DOTMP have been developed. Availability of this kit is expected to stimulate the widespread use of $^{68}Ga$-DOTMP in the fields of nuclear medicine.

• 분석과학
• /
• 제34권4호
• /
• pp.160-171
• /
• 2021

Synthetic azo dyes are used extensively in herbal medicines to render the medicines more visually attractive to consumers. This study developed and validated a rapid high-performance liquid chromatography (HPLC) method to determine whether synthetic colorants such as Tartrazine, Auramine O, Metanil yellow, Sunset yellow, and Orange II are used extensively in Typha orientalis. To increase the recovery of the synthetic dyes, this method employed containing 50 mM ammonium acetate in 70 % methanol at first extraction and 100 mM HCl in 70 % methanol at second extraction. Five synthetic pigments in Typha orientalis were separated by gradient elution with a mobile phase consisting of acetonitrile and 50 mM ammonium acetate in distilled water at ultra-violet (UV) detection 428 nm or 500 nm. Additionally, this study established the liquid chromatography tandem mass spectrometry (LC-MS/MS) method to confirm positive samples suspected by HPLC results. The HPLC-UV method had good linearity, indicating r²> 0.999. The recoveries of the samples spiked with three different concentration ranged from 73.8~91.5 %, and relative standard deviation values indicated 0.2~5.2 %. The established LC-MS/MS could successfully identify the synthetic pigments in herbal medicine samples. The study demonstrates that Typha orientalis adulterated by yellowish synthetic dyes can be successfully distinguished when using the HPLC-UV method.

• 생명과학회지
• /
• 제22권5호
• /
• pp.575-581
• /
• 2012

본 연구는 가축용 생균제로서 이용성이 우수한 $Lactobacillus$ $plantarum$과 $Lactobacillus$ $reuteri$의 대량생산을 위한 경제적 최적 배지조성을 도출하기 위하여 수행되었다. 두 균주의 배양을 위한 배지성분 요인들은 유산균 배지로 알려진 MRS broth를 기준으로 하여, sucrose, glucose, molasses, yeast extract, corn steep liquor, soy peptone, dipotassium phosphate, manganese chloride, magnesium chloride, tween 80, sodium chloride, sodium acetate, ammonium citrate, sodium sulphate 및 ferrous sulphate 등 총 15가지를 선택하였다. 각 배지성분들이 각 균주들의 세포성장에 미치는 요인분석은 20개의 실험으로 구성된 Plackett-Burman design 방법을 이용하여 분석하였다. 그 결과 $L.$ $plantarum$의 성장에 있어서는 sucrose, glucose, molasses, yeast extract, corn steep liquor, soy peptone, sodium acetate, ammonium citrate 등이 긍정적인 효과(positive effect)를 나타내었다. $L.$ $reuteri$의 경우, yeast extract, soy peptone, $K_2PHO_4$, tween 80에서 수준간의 격차가 큰 긍정적인 효과가 나타났다. 두 가지 균주들에서 얻어진 표준화된 통합적 효과의 주 효과를 분석한 결과, sucrose, yeast extract, soy peptone에서 긍정적인 효과가 나타났다. 최종적으로 $L.$ $plantarum$과 $L.$ $reuteri$ 두 균주에 공통적으로 적용할 수 있는 유효 배지 성분들로 sucrose (20.0 g/l), glucose (5.0 g/l), soy peptone (11.0 g/l), yeast extract (5.0 g/l), $K_2PHO_4$ (0.2 g/l), $CH_3COONa$ (2 g/l) 및 $MgCl_2$ (0.02 g/l)를 선택하였다.