• Biomolecules & Therapeutics
• /
• 제16권2호
• /
• pp.168-172
• /
• 2008

The present study is to determine of sensitive nicorandil analysis method using HPLC and measure the pharmacokinetics parameters (bioavailability, $C_{max}$, $T_{max}$, Ke, $T_{1/2}$) of nicorandil (5 mg, Tab; Choongwae Pharma Corporation). Plasma (500 ul) was mixed with furosemide (internal standard, 500 ug/ml). Detection wavelength was 256 nm. The mixture of 0.01 M ammonium acetate and acetonitrile 80:20 (v/v) was used mobile phase. The HPLC separation was accomplished on ODC reverse HPLC column. The nicorandil was analyzed by a HPLC system, which consists of CAPCELL PAK C18 column (5 ${\mu}$m, 4.6 × 150 mm) and a chromatography data analysis S/W, using a isocratic mobile phase (mixture of 0.01 M ammonium acetate and acetonitrile 80:20 ) at 1.0 ml/min. Its sensitivity, selectivity, accuracy and precision must be adequate for the bioavailabilty study of nicorandil, and the linearity ($r^2$ ≥ 0.9994) of nicorandil was also proved in the range of 0.05 ug/ml . 3 ug/ml. The pharmacokinetic parameters of nicorandil (5 mg) tablets were measured as the follow. AUC: 0.19 ug/ml·hr, $C_{max}$: 0.14 ug/ml, $t_{max}$: 0.58 hr, Ke: 0.11 hr., $t_{1/2\beta}$: 6.76 hrs. This method is simple and sensitive HPLC method using UV detector for determination of nicorandil in human plasma.

• 한국응용과학기술학회지
• /
• 제30권4호
• /
• pp.664-671
• /
• 2013

역상 HPLC 에 의한 화장품에 사용하는 수용성 고분자인 Xanthan gum의 간편하고 정확한 정량분석 방법을 개발하였다. 분석조건은 $C_{18}$분리관과 ELSD 검출기를 사용하였으며, 25mM ammonium acetate/acetonitrile의 기울기 용리에 의해 분리 되었다. 이때 검량선의 상관계수(correlation coefficient) $r^2=0.9993$, 정량범위 $50.3{\sim}604.1{\mu}g/ml$, 검출한계 $12.0{\mu}g/ml$ 및 정확성이 우수하였다. 이 방법은 화장품 중 Xanthan gum를 정확하고 간편하게 정량분석 가능함을 보여 주었다.

• 한국동물위생학회지
• /
• 제18권3호
• /
• pp.13-28
• /
• 1995

This study was carried out to explore the most sensitive and useful method for simultaneous determination of five sulfa drugs(sulfamethazine, sulfamerazine, sulfamonomethoxine, sulfadimethoxine, sulfaquinoxaline) in livestock productions(pork muscle, bovine muscle, chicken muscle, milk ) by HPLC with UV detector and reverse phase column. The results obtained were as follows：1. For mobile phase acetonitrile-0.01M ammonium acetate (23：77) showed more applicable sensitivity and retention times than acetonitrile-1% acetic acid(23：77). Thus acetonitrile-0.01M ammonium acetate(23：77) selected and applied to the modification test, from which it was found pH 6.75 was the most adequate. 2. Optimal wavelength of UV for SMT(sulfamethazine), SMR(sulfamerazine), SMM(sulfamonomethoxine), SD(sulfadimethoxine), and SQ(sulfaquinoxaline) were 266, 266, 265, 269 and 250nm, respectively, and that for simultaneous application it was 263nm. 3. The average recovery rate by extractant(chloroform, dichloromethane, chlorform+dich-loromethane) in the classic method was not significantly different(p>0.05) but that by chloroform higher than the others. Thus chloroform was found to be adequate as extractant in this classic method. 4. The average recovery rate was 86.5% by the MSPD(matrix solid phase disperse) method, which was significantly higher than that by the classic method(p<0.05). Also the recovery rates by method were significantly different(p<0.05) in accordance with sample and type of drug. The MSPD method was much superior to classic method on clean-up.

• 유기물자원화
• /
• 제13권2호
• /
• pp.121-127
• /
• 2005

퇴비로부터 종속영양 질산화과정에 관여하는 세균을 분리하여 그 특성을 조사하고 동정하였다. 본 세균은 그람 양성 간균으로 운동성을 가지고 있었으며, ammonium acetate 배지에서 아질산용액으로 실험한 결과, 세균의 군락이 자주색으로 변화됨으로써 암모니아로부터 아질산을 형성하는 것으로 나타났다. 세균의 동정을 위한 BBL Test의 결과 본 세균은 Bacillus strains과 78%의 유사성을 지닌 것으로 나타났다. 계통분류학적 분석을 위하여 세균의 염색체로부터 16S rDNA를 클로닝한 후 DNA 염기서열을 파악하고 이를 비교 및 분석한 결과 본 세균은 Bacteria; Actinobacteria; Actinobacteridae; Actinomycetales; Corynebacterineae; Mycobacteriaceae; Mycobacterium에 속하는 Mycobacterium smegmatis strain과 94%의 유사성을 보이는 것으로 나타났다.

• 한국미생물·생명공학회지
• /
• 제20권2호
• /
• pp.183-189
• /
• 1992

Bacteriocin 생산균주인 Lactococcus sp. 111-2의 최적 배양조건과 배양 특성을 검토하였다. 탄소원은 glucose 20g/l, 질소원은 casein acid hydrolyzate 15g/l, 무기염류는 sodium acetate 3g/l와 ammonium citrate 2g/l가 bacteriocin 생성에 많은 영향을 주는 것으로 타났으며, 배지 pH는 6.2, 발효온도는 $35^{\circ}C$일 때 높은 생성수율을 보였다. 선발균주는 증식 및 항균물질의 생성에 riboflavin과 pantotheinc acid의 비타민을 요구하였다. 회분식 발효시 항균성물질의 생산패턴은 배양 12시간에 3,894IU/ml로 최대를 나타냈으며 균체생성은 배양 8.5시간에 정상기에 도달하였다.

• 대한화학회지
• /
• 제51권6호
• /
• pp.520-525
• /
• 2007

Thiourea/urea, 다양한 aromatic aldehydes, ammonium acetate의 세가지 요소를 가지고 마이크로파의 조 사하에서 무수조건의 활성화된 비산화재를 촉매로 사용하여 높은 수율의 6-aryl-1,2,4,5-tetrazinan-3-thiones/ones를 얻었 다. 6-aryl-1,2,4,5-tetrazinan-3-thiones/ones의 구조는 녹는점, MS, IR, 1H NMR, D2O 교환, 13C NMR, 이차원의 NMR 스펙트럼(HOMOCOR, HSQC)을 바탕으로 증명되었다.

• KSBB Journal
• /
• 제25권5호
• /
• pp.459-465
• /
• 2010

A simple and efficient analytical method for the simultaneous determination of seven tar color additives was developed using ion pair high performance liquid chromatography. The conditions for HPLC analysis were as follows: column, ${\mu}$-Bondapak C18 (10 ${\mu}m$, 300 ${\times}$ 3.9 mm i.d.); gradient mobile phase, 0.025 mol/L ammonium acetate (containing 0.01 mol/L tetrabutylammonium bromide)-acetonitrile-methanol (65:25:10) as a mobile for fraction A and 0.025 mol/L ammonium acetate (containing 0.01 mol/L tetrabutylammonium bromide)-acetonitrilemethanol (40:50:10) as a mobile for fraction B; flow rate, 1.0 mL/ min; detection wavelength, 254/520/620 nm. We could attain to the detection limits as 0.01~0.05 ${\mu}$g/mL (254 nm) and 0.005~0.01 ${\mu}$g/mL (520 nm) for six red tar color additives, and 0.05 ${\mu}$g/mL (254 nm) and 0.002 ${\mu}$g/mL (620 nm) for Fast green FCF. This analytical method was applicable to determine the tar color additives contained in several commercial cold syrups.

• 대한약침학회지
• /
• 제9권2호
• /
• pp.105-111
• /
• 2006

Objectives : This study was conducted to carry out Purification of Melittin and other peptide components from Bee Venom using gel filtration chromatography and propionic acid/urea polyacrylamide gel electrophoresis Methods : Melittin and other peptide components were separated from bee venom by using gel filtration chromatography on Sephadex G-50 column in 0.05M ammonium acetate buffer. Results : Melittin and other peptide components were separated from bee venom by using gel filtration chromatography on Sephadex G-50 column in 0.05M ammonium acetate buffer. The fractions obtained from gel filtration chromatography was analyzed by using SDS-PAGE and propionic acid/urea polyacrylamide gel electrophoresis. The melittin obtained from the gel filtration contained residual amount of phospholipase $A_2$ and a protein with molecular weight of 6,000. The contaminating proteins were removed by the second gel filtration chromatography. Conclusion : Gel filtration chromatography and propionic acid/urea polyacrylamide gel electrophoresis are useful to separate peptide components including melittin from bee venom.

• 한국식품과학회지
• /
• 제34권6호
• /
• pp.1145-1148
• /
• 2002

뇌의 송과선에서 분비되는 호르몬인 melatonin은 생체의 산화적 손상을 방어하는 효능이 있다고 알려져 있는데, 26종의 식물체로부터 그 존재 및 함량을 분석하였다. Melatonin 함유 분획을 조제하기 위하여 동결건조된 식물체 분말을 50% ethanol로 추출한 다음 여과 후 TLC 상에서 acetone으로 전개시켜 melatonin을 분리하였다. 형광하에서 확인된 melatonin spot은 methanol로 추출한 다음 HPLC로 분석하였다. HPLC 분석시 ODS-A column을 사용하였으며 이동상은 70% ammonium acetate와 메탄올을 30 : 70(v/v)으로 섞어서 1.0 mL/min의 속도로 흘려주었다. Melatonin peak의 확인은 형광 detector를 이용하였으며 retention time은 17분이었다. 이같은 분석조건에서 셀러리, 부추, 브로콜리, 컬리플라워 등에서 높은 melatonin 함량이 확인되었다.

• 한국막학회:학술대회논문집
• /
• 한국막학회 2004년도 추계 총회 및 학술발표회
• /
• pp.263-266
• /
• 2004

An experimental study was carried out on the recovery of ammonium lactate from simulated fermentation brothby desalting electrodialysis (DSED). All experiments, using AM-1 and CM-1 membranes, were operated in a constant current and a subsequent constant-voltage mode, and the switching point was determined based on the previous results. The effects of operating conditions such as operating current, initial feed concentration, and initial feed pH were investigated. Increased operating current resulted in a decreased operating time but an increased energy consumption per unit amount of ammonium lactate recovered. As the initial feed concentration was increased, the operating time increased while energy consumption decreased. The operating time and energy consumption slightly decreased as the initial feed pH was increased up to 6.0. However, no significant influences on the recovery of ammonium lactate were observed over 6.0. The rejections of acetate, glucose, and proteins were $10\%,\;93\%,\;and\;98\%$, respectively. Sulfate was not rejected at all.