Browse > Article

Analysis of Melatonin Content from Domestic Edible Plants  

Kim, Seok-Joong (Department of Food Science and Technology, Catholic University of Daegu)
Publication Information
Korean Journal of Food Science and Technology / v.34, no.6, 2002 , pp. 1145-1148 More about this Journal
Abstract
Melatonin, which is a hormone secreted from pineal gland of brain and known to prevent oxidative damages of various tissues, was analyzed in 26 domestic edible plants. For the preparation of melatonin fraction, 50% ethanol extract prepared from lyophilized plant powder was filtered and applied on TLC plate. Melatonin position on TLC developed with acetone was identified by fluorescence light and extracted with methanol. This methanolic fraction was injected into HPLC comprising ODS-A column, fluorescence detector, and mobile phase consisting of a mixture (30 : 70, v/v) of 70% ammonium acetate and methanol at a flow rate of 1.0 mL/min. Melatonin was identified at the retention time of 17 min. Results revealed that celery, leek, broccoli, and cauliflower had higher melatonin contents than others.
Keywords
melatonin; plant; HPLC;
Citations & Related Records
연도 인용수 순위
  • Reference
1 Tan, D.X., Poeggeler, L.D., Manchester, L.D. and Reiter, R.J. Melatonin: A potent endogeneous hydroxyl radical scavenger. Endocr.J. 1:57-60 (1993)
2 Dubbels, R., Reiter, R.J., Klenke, E., Goebel, A., Schnakenberg,E., Ehlers, C., Schiwara, H.W. and Schloot, W. Melatonin in edible plants identified by radioimmunoassay and by high performance liquid chromatography-mass spectrometry. 18: 28-31(1995)
3 Kim, S.J., Reiter, R.J., Rouvier-Garay, V., Qi, W., El-Sokkary, G.M. and Tan, D.X. 2-Nitropane-induced lipid peroxidation: antitoxic effects of melatonin. Toxicol. 130: 183-190 (1998)   DOI   ScienceOn
4 Livrea, M.A., Tesoriere, L., D'Arpa, D. and Morreale, M. Reaction of melatonin with lipoperoxyradicals in phospholipid bilayers. Free Radical Biol. Med. 23: 706-711 (1997)   DOI   ScienceOn
5 Kim, S.J., Qi, W., Tan, D.X., Cabrera, J. and Reiter, R.J. Melatonin prevents oxidative damage to protein and lipid induced by ascorbate-$Fe^{3+}$-EDTA: Comparison with glutathione and $\alpha$-toco-pherol. Neuroendocrinol. Lett. 21: 269-276 (2000)
6 Reiter, R.J., Pablos, M.I., Agapito, T.T. and Gerrero, J.M. Melatonin in the context of the free radical theory of aging, Pharmacol Int. Aging Age-Asso. Dis. 15: 362-378 (1996)
7 Stasica, P., Ulanski, P. and Rosiak, J.M. Melatonin as a hydroxyl radical scavenger. J. Pineal Res. 25: 65-66 (1998)   DOI   PUBMED
8 Reiter, R.J. The pineal gland and melatonin in relation to aging: A summary of the theories and of the data. Exp. Gerontol. 30: 199-212(1995)
9 Hattori, A., Migitaka, H., Igio, M., Itoh, M., Yamamoto, K., Ohtani-Kaneko, R., Hara, M., Suzuki, T. and Reiter, R.J. Identification of melatonin in plants and its effects on plasma melatonin levels and binding to melatonin receptors in vertebrates. Biochem. Mol. Biol. Int. 35: 627-634 (1995)   PUBMED
10 Brzezinski, A., Melatonin in humans. New Eng. J. Med. 336: 186-195(1997)   DOI   ScienceOn
11 Gilad, E., Cuzzocrea, S., Zingarelli, B., Salzman, A.L. and Szab$\, C. Melatonin is a scavenger of peroxynitrite. Life Sci. 60: PL169-174 (1997)
12 Reiter, R.J., Tan, D.X., Kim, S.J., Manchester, L.C., Qi, W., Garcia, J., Cabrera, J., El-Sokkary, G. and Rouvier-Garay. V. Augmentation of indies of oxidative damage in life-long melatonin-deficient rats. Mech. Aging Dev. 110: 157-173 (1999)   DOI   ScienceOn
13 Cagnoli, C.M., Atabay, C., Kharlamova, E. and Manev, H. Melatonin protects neurons from singlet oxygen-induced apoptosis. J. Pineal Res. 18: 222-226 (1995)   DOI   ScienceOn
14 Murch, S.J., Simmons, C.B. and Saxena, P.K. Melatonin in feverfew and other medicinal plants. Lancet 350: 1598-1599 (1997)   DOI   ScienceOn
15 Reiter, R.J. and Kim. S.J. Phytochemicals: melatonin. pp. 1918-1922. In: Encyclopedia of Food Science and Technology, Francis, F.J. (ed.). John Wiley, New York, USA (1999)
16 Kehrer, J.P. and Smith, C.V. Free radicals in biology: Sources, reactivities, and roles in the etiology of human diseases, pp. 25-62. In: Natural Antioxidants in Human Health and Disease, Frei, B. (ed.). Academic Press, London, UK (1994)
17 Reiter, R.J., Tan, D.X., Kim, S.J. and Qi, W. Melatonin as a pharmacological agent against oxidative damage to lipids and DNA. Proc. West. Pharmacol. Soc. 41, 229-236 (1998)   PUBMED