• Proceedings of the Korean Society of Crop Science Conference
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• 2017.06a
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• pp.89-89
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• 2017

A gene encoding squalene synthase from grain amaranth was cloned and characterized. The full-length cDNA was 1805-bp long and contained a 1248-bp open reading frame encoding a protein of 416 amino acids with a molecular mass of 47.6 kDa. Southern blot analysis revealed that the A. cruentus genome contained a single copy of the gene. Comparison of the cDNA and genomic sequences indicated that the amaranth SQS gene had 12 introns and 13 exons. All of the exons contributed to the coding sequence. The predicted amino acid sequence of the SQS cDNA shared high homology with those of SQSs from several other plants. It contained conserved six domains that are believed to represent crucial regions of the active site. We conducted qRT-PCR analyses to examine the expression pattern of the SQS gene in seeds at different developmental stages and in several tissues. The amaranth SQS gene was low levels of SQS transcripts at the initial stage of seed development, but the levels increased rapidly at the mid-late developmental stages before declining at the late developmental stage. These findings showed that the amaranth SQS is a late-expressed gene that is rapidly expressed at the mid-late stage of seed development. In addition, we observed that the SQS mRNA levels in stems and roots increased rapidly during the four- to six-leaf stage of development. Therefore, our results showed that the expression levels of SQS in stem and root tissues are significantly higher than those in leaf tissues. In present study provides useful information about the molecular characterization of the SQS clone isolated from grain amaranth. Finally, a basic understanding of these characteristics will contribute to further studies on the amaranth SQS.

• Korean Journal of Weed Science
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• v.10 no.4
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• pp.305-311
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• 1990

Effects of light on germination of major weeds in upland fields were examined. Most weeds showed light positive response to germination at above 25$^{\circ}C$, but germination percentage was very low at lower temperatures. Red light promoted germination of Amaranthus lividus, Portulaca oleracea, and A. retroflexus, but far-red light inhibited germination. Germination resposnse of A. retroflexus, A. lividus, and P. oleracea showed reversible reaction to red/far-red light, and thus it seemed that phytochrome was involoved in seed germination, Echinochloa crusgali and Eleusine indica showed higher germination percentage under the conditions of continuous illumination of red, white, and bule lights, and thus it was estimated that blue light pigment (HIR, high irradiation reaction) related to long time illumination was involved in germination in addition to phytochrome.

• Korean Journal of Weed Science
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• v.16 no.4
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• pp.259-263
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• 1996

Occurrence and distribution of weeds were surveyed in autumn vegetable crops(Chinese cabbage, radish, cucumber and Welsh onion) in 16 cities or counties of Chungnam area during October and November 1995. Thirty weed species were occurred, and Rorippa globosa, Alopecurus aequalis var. amurensis, Stellaria media, Portulaca oleracea, Digitaria sanguinalis and Chenopodium album var. centrorubrum showed higher importance values among them. S. media, P. oleracea and A, aequalis var. amurensis were dominant in northwest coastal area, R globosa, D. sanguinalis and S. media in southwest coastal area, R globosa, A. aegualis var. amurensis and D. sanguinalis in northern area, and R globosa, A. aequalis var. amurensis and P. oleracea in southern area. S. media, R globosa and P. oleracea were dominant in Chinese cabbage fields, R globosa, Amaranthus retroflexus and S. media in radish fields, A. retroflexus, S. media and D. sanguinalis in cucumber fields, and R. globosa and P. oleracea in Welsh onion fields.

• Korean Journal of Weed Science
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• v.18 no.3
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• pp.214-224
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• 1998

This study was conducted to develop labor and chemical cultural practice improvement for weed control in ginseng field. Five crop species were screened in vitro and three crop extracts were sprayed in the test allelopathic effect for weed species. Methyl alcohol extracts of rice, oat, rye and wheat straw showed inhibitory effect on the germination of six weed species, Amaranthus retroflexus, Chenopodium album, Arthraxon hispidus, Digitaria adscendens, Echinochloa crus-galli, and Setaria viridis. Especially, extract of oat straw was most effective to inhibit the germination of six weed species tested. Also, spraying the extract solution of rice, rye and wheat straw was effective to inhibit the growth of weeds in ginseng field. Ginseng root length, diameter, fresh and dry weight in extract spraying plot were higher than those of non-treatment plot.

• Journal of Haehwa Medicine
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• v.29 no.2
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• pp.22-29
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• 2020

Objectives: This study aims to investigate the effects of diabetes mellitus care mixture (DCM) on blood glucose and lipid metabolism in type 2 diabetic mellitus mice. DCM consisted of lagerstroemia speciose, allium hookeri, momordica charantia, amaranthus tricolor, and boesenbergia rotunda, which have been proven to have antidiabetic properties. Methods: In this study, we researched the effects of DCM in type 2 diabetic mellitus mice. C57BLKS/J mouse groups had no treatment, db/db mouse randomly assigned to 2 groups, and treated with distilled water and DCM (200 mg/kg/day). Blood glucose levels and body weight were checked every week. After 4 weeks of treatment, liver function indicators (AST, ALT, and LDH) and lipid metabolites (triglyceride, total cholesterol, LDL-cholesterol, HDL-cholesterol) were measured with a biochemistry analyzer. Diabetic factors (insulin, resistin, and leptin) were measured with ELISA. Results: DCM was decreased blood glucose, diabetic factors, liver function indicators, triglyceride, total cholesterol, and LDL-cholesterol significantly. Also, HDL-cholesterol was significantly increased in DCM group. The bodyweight of DCM group decreased but, no significant difference with the control group. DCM may have the potential to improved diabetes mellitus by regulating blood glucose levels and diabetic factors. Also protecting from diabetic complications by adjusting liver function indicators and lipid metabolites. Conclusions: These results suggest that DCM to be used as an oriental medicine for diabetes, the results of clinical trials are needed.

• Weed & Turfgrass Science
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• v.5 no.2
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• pp.65-70
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• 2016

Investigation on weed flora in Angelica gigas upland fields was conducted to understand the current status of the weed flora and establish the control measures. Investigation was conducted twice, July and October in 2015. From this investigation, 105 species of 37 families including 27 exotics were identified and classified into 53 species of annuals, 24 species of biennials and 28 species of perennials. Dominance was the highest with Digitaria ciliaris, followed by Portulaca loeracea, Chenopodium album, Cyperus iria, Conyza canadensis, Galinsoga ciliata etc. in order. Chenopodium album was the highest in importance analysis and the followings were in order of Conyza canadensis, Galinsoga ciliata, Amaranthus lividus, Taraxacum offcinale etc. The yield of A. gigas was reduced 49.6% in no weeding plots comparing in weed managed plots.

• Weed & Turfgrass Science
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• v.5 no.2
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• pp.60-64
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• 2016

Investigation on weed flora in Lycium chinense upland fields was conducted to understand the current status of weed flora and establish the control measures. Investigation was conducted twice, May and June on 2015. From this investigation, 91 species of 30 families including 27 exotics were identified into 36 species of annuals, 28 species of biennials and 27 species of perennials. Dominance was the highest with Portulaca loeracea followed by Digitaria ciliaris, Stellaria media, Oxalis corniculata, Acalypha australis, Chenopodium album, Cyperus iria etc. in order. Chenopodium album was the highest in importance analysis and the followings were in order of Conyza canadensis, Conyza bonariensis, Senecio vulgaris, Amaranthus lividus, Sonchus asper etc.

• Korean Journal of Environment and Ecology
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• v.19 no.1
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• pp.31-45
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• 2005

This study was carried out to investigate the vascular plants of the construct-reserved sites of ecological forests in Deokdong-ri, Baekwoon-myeon, Jecheon-si, and Chungcheongbuk-do, Korea and the period was from March to October, 2004. The results of surveying the vascular plants were recorded as 371 taxa; 83 families, 238 genera, 324 species, 44 varieties and 3 forma. There were 4 species of rare and endangered plants as designated by Korea Forest Service; Eranthis stellata, Paeonia japonica, Viola albida and Scopolia japonica. There were 9 species of Koreanendemic plants Pseudostellaria coreana, Melandryum seoulense, Clematis trichotoma, Deutzia coreana, Spiraea prunifolia for. simpliciflora, Ajuga spectabilis, Weigela subsessilis, Cirsium setidens and Saussurea nutans. Ten species of naturalized plants were observed Panicum dichotomiflorum, Rumex crispus, Amaranthus retroflexus, Lepidium apetalum, Trifolium repens, Oenothera Jamayckiana, Erigeron annuus, Erigeron canadensis, Bidens frondosa and Taraxacum officinale. In the results of analysis by floristic region, the specific plants were summarized as 52 taxa; 30 families, 47 genera, 49 species and 3 varieties.

• Korean journal of applied entomology
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• v.43 no.1
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• pp.35-41
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• 2004

Bacillus thuringiensis produces crystal proteins toxic to medically and agriculturally important pests during sporulation. To improve the activity of insecticidal crystal protein in applying to mosquito larval control, an expression vector, pSyn4D harboring the mosquitocidal cry11Aa gene under control of psbA promoter of Amaranthus hybridus was constructed. This expression vector was transformed into Synechocystis PCC6803 and a transformant, Tr2C was selected with kanamycin. The mosquitocidal cry11Aa gene was stably integrated Into genomic DNA of Tr2C in PCR detection using cry11Aa-specific primers. The transformant expressed 72-kDa Cry11Aa protein and median lethal time (LT$\sub$50/) was approximately 2.1 days for Culex tritaeniorhynchus larvae and 0.7 day for Anopheles sinensis larvae, respectively. These results suggest this transformant can be used for mosquito larval control as a biological control agent.

• Journal of the East Asian Society of Dietary Life
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• v.26 no.5
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• pp.466-472
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• 2016

The objectives of the study were to analyze the various nutrient contents of ready-to-use-supplemental-food (RUSF) developed with Eastern African ethnic plants and compare them with commercially available supplemental food (CASF) for infants. RUSF was made with three African plants, including Ficus sur, Amaranthus caudatus, and Prosopis juliflorafa and locally available food ingredients in DR Congo and Ethiopia and was cooked at $180^{\circ}C$ for 20 min in a conventional oven. Macronutrients were analyzed by the AOAC method. Contents of 17 amino acids were analysed with amino acid analyzer, and vitamin $B_1%$ niacin, vitamin $B_6$, and vitamin C were analyzed with HPLC. Isomers of vitamin E were analyzed by GC-FID. The price of RUSF was 0.133 USD per 100g. Contents of macronutrients were not significantly different between developed RUSF and CASF. Results of amino acid analysis showed that most amino acid contents were similar or higher in RUSF compared with CASF. However, glutamic acid and lysine contents were less in RUSF than in CASF. Vitamin E and vitamin $B_1$ contents were more than five times higher in RUSF (22.40, 6.26 mg/100 g, respectively) than in CASF (4.00, 0.80 mg/100 g, respectively). Niacin was about three times higher while vitamin $B_6$ was almost 20 times higher in RUSF than in CASF. However, ascorbic acid was significantly lower in RUSF (3.43 mg/100 g) than in CASF (42.08 mg/100 g). Our study found that the developed localized RUSF showed higher nutrient contents in most micronutrients analyzed except for ascorbic acid. Out of 17 amino acids, glutamic acid and lysine contents in RUSF were less than in CASF. Therefore, further study is needed in order to improve these nutrients contents.