• Title/Summary/Keyword: AmA1

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Autocatalytic Cure Kinetics of DGEBA/MDA/PGE-AcAm System (DGEBA/MDA/PGE-AcAm계의 자촉매 반응 속도론)

  • Lee, Jae-Yeong;Sim, Mi-Ja;Kim, Sang-Uk
    • Korean Journal of Materials Research
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    • v.8 no.9
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    • pp.797-801
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    • 1998
  • The cure kinetics for diglycidyl ether of bisphenol A(DGEBA)/4, 4'-methylene dianiline(MDA) system with or without lOphr of phenyl glycidyl ether(PGE)-acetamide(AcAm) was studied by autocatalytic cure expression. On the dynamic DSC curves, the exothermic peak temperature and the onset temperature of reaction decreased with the addition of PGE-AcAm. Regardless of the addition of PGE-AcAm, the shape of the conversion curve showed sigmoid, and this meant that DGEBA/MDA and DGEBA/MDA/PGE-AcAm systems followed autocatalytic cure reaction. When PGE-AcAm was added to DGEBA/MDA system, the cure rate increased about 1.2~1.4 times due to the catalytic role of hydroxyl groups in PGE-AcAm.

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Expression of AmA1 Gene Encoding Storage Protein of Amaranthus in Transgenic Tobacco (형질전환 담배에서 Amaranthus 저장단백질인 AmA1 유전자의 발현)

  • 김태금;김영숙;권태호
    • Korean Journal of Plant Tissue Culture
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    • v.27 no.3
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    • pp.169-173
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    • 2000
  • A 1,183bp cDNA, AmA1, encoding the seed storage protein of Amaranthus hypochondriacus was isolated by reverse transcriptase-polymerase chain reaction (RT-PCR) and characterized. AmA1 gene was subcloned into plant binary vector under Cauliflower Mosaic Virus (CaMV) 35S promoter and nopaline synthase terminator (3'NOS). The recombinant binary vector was used to transform Nicotiana tabacum using Agrobacterium tumefacien -mediated transformation procedure. Shoots were induced on MS medium with 0.1 mg/L NAA, 1.0 mg/L BA, 100 mg/L kanamycin and 250 mg/L cefotaxime. Transgenic plants were selected on rooting medium based on MS medium containing 200 mg/L kanamycin and 250 mg/L cefotaxime without phytoregulators. The presence of AmA1 gene in the transgenic plants was confirmed by PCR followed by DNA hybridization. The expression of AmA1 gene in the transgenic plant was observed by RT-PCR method.

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Effects of PGE-AcAm on the Tensile and Impact Properties of DGEBA/MDA System (DGEBA/MDA계의 인장 및 충격 특성에 미치는 PGE-AcAm의 영향)

  • Lee, Jae-Yeong;Sim, Mi-Ja;Kim, Sang-Uk
    • Korean Journal of Materials Research
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    • v.7 no.6
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    • pp.460-463
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    • 1997
  • 에폭시 수지 계의 경화반응 속도를 증가시키고 기계적 물성을 향상시키기 위해 합성 pheny1 glycidy1 ether(PGE)-acetamide(AcAm)를 diglycidy1 ether of bispenol A(DGEBA)/4,4'-methylene dianiline(MDA)계에 도입하였다. PGE와 AcAm을 2:1의 몰비로 혼합한 후 18$0^{\circ}C$에서 1시간 반응시켜서 PGE-AcAm을 합성하였다. 5phr의 PGE-AcAm이 첨가되었을 때 인장강도가 15% 개선되었으며, 그 이후로는 PGE-AcAm을 합성하였다. 5phr의 PGE-AcAm이 첨가되었을 때 인장강도가 15% 개선되었으며, 그 이후로는 PGE-AcAm의 함량에 관계없이 거의 비슷한 값을 나타내었다. 반면에유리전이 온도(Tg)와 충격강도는 PGE-AcAm의 함량이 증가함에 따라 감소하였다. 파단면은 PGE-AcAm이 첨가됨으로써 더 복잡한 형상을 나타내었다.

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Analysis of Data Spectral Regrowth from Nonlinear Amplification

  • Amoroso, Frank;Monzingo, Robert A.
    • Journal of Communications and Networks
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    • v.1 no.2
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    • pp.81-85
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    • 1999
  • The regrowth of OQPSK power spectral sidelobes from AM/AM and AM/PM amplifier nonlinearity is analyzed. The time-domain expression for amplifier output shows how spectral re-growth will depend on the cubic coefficient of the Taylor's series of the amplifier nonlinearity as well as input amplitude ripple. Closed form spectrum calculations show that the spectral sidelobes produced by AM/PM take the same form as those produced by AM/AM. The rate of growth of AM/PM sidelobes is, however, not as great as for AM/AM.

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Implicit D-Ary AM-Heap (묵시 다원 AM-힙)

  • Jung, Haejae
    • KIPS Transactions on Computer and Communication Systems
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    • v.7 no.12
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    • pp.289-294
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    • 2018
  • This paper proposes an implicit d-ary priority queue, called AM(d)-heap that is a generalized version of AM-heap, in which insert operation takes constant amortized time and remove operation takes O(logn) time. According to our experimental results, the best performance was shown when d is 4 or 8. Also, AM(d)-heap is about 1.5~1.8 times faster than the postorder heap.

Effects of Dietary Herb Products(Animunin Powder$^{?}$) on Egg Characteristic, Blood Components, and Nutrient Digestibility in Laying Hens (허브제품(Animunin Powder$^{?}$)의 급여가 산란계의 계란품질과 혈액성상 및 영양소 소화율에 미치는 영향)

  • Shon K. S.;Kwon O. S.;Min B. J.;Cho J. H.;Chen Y. J.;Kim I. H.;Kim H. S.
    • Korean Journal of Poultry Science
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    • v.31 no.4
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    • pp.237-244
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    • 2004
  • This study was conducted to investigate the effects of dietary Animunin Powder$^{?}$ on the egg quality characteristics, blood components and nutrient digestibility in laying hens. A total of two hundred seventy laying hens were randomly allocated into three treaments with fifteen replications for eight weeks. Dietary treaments included 1) Control (CON), 2) Control + $0.1\%$ Animunin Powder$^{?}$ (AM1), 3) Control + $0.2\%$ Animunin Powder$^{?}$ (AM2). During the period of 0~4weeks, the birds fed the AM1 diet had an improved egg production compared to the birds fed the CON (P<0.05). During the period of 4~8weeks the birds fed AM1 diet showed a statistically improved egg production compared to the CON (P<0.05). However, no significant differences were founded in the egg weight. During the period of $4\~8$ weeks the hens fed the AM2 diet had improved egg yolk color compared to the hens fed CON and AM1 diets (P<0.05). In the Haugh unit for the period of $0~4$$weeks, the AM2 treatment showed significantly improved results compared to the CON (P<0.05). Average egg shell breaking showed no significant differences through the experiment period, but in the period of $4\~8$weeks the AM2 treatment tended to be improved compared to the CON and AM1 treatment (P<0.05). There was no significant differences in egg shell thickness. In the serum cholesterol, the AM1 and AM2 treatments were significantly lower than the CON (P<0.05). The concentration of RBC and WBC in the AM treatments tended to increase but there were no significant differences. For the differences of lymphocytes between the end and initiation of the experiment, the hens fed the AM1 treatment were significantly different compared to the hens fed the CON and AM2 treatment (P<0.05). During the period of the experiment, the hens fed the AM1 diet were tended to show higher DM digestibility than the hens fed the CON and AM2 diet, but it was not statistically different. In conclusion, dieatry fed of Animunin Powder$^{?}$ could improve egg production, egg yolk color, and haugh unit.

다상포낙선검파법에 관한 연구

  • 이충웅
    • Journal of the Korean Institute of Telematics and Electronics
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    • v.10 no.1
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    • pp.18-26
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    • 1973
  • In AM detection system, when the ratio of the frequency of the carrier to that of the modulating signal becomes less than a ratio of approximately 10:1, AM detectors become unable to detect the impressed signal in a satisfactory waveform. The failure of detecting such a super wide-band AM signal resulting from charging and discharging transient phenomena in associated diode circuit during the detecting of AM signal, has been a cause of limiting the channel capacity in AM or FM super multiplexing systems. This Paper presents the "Poly-phase Envelope Detection System" as a suitable method to solve the problem and the analyses of the system suggested. This system will make it possible to take the envelope out of the impressed AM signal to any desired degree of accuracy even when the ratio of the frequency of the carrier to that of the modulating signal approaches unity. Experiments were carried out to verify the validity of the theory of Poly-phase Envelope Detection System by adopting the frequency conversion method from among the two proposals.proposals.

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Expression of Nutritionally Well-balanced Protein, AmA1, in Saccharomyces cerevisiae

  • Kim, Tae-Geum;Kim, Ju;Kim, Dae-Hyuk;Yang, Moon-Sik
    • Biotechnology and Bioprocess Engineering:BBE
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    • v.6 no.3
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    • pp.173-178
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    • 2001
  • Food yeast, Saccharomyces cerevisiae, is a safe organism with a long history of use for the production of biomass rich in high quality proteins and vitamins. AmA1, a seed storage albumin from Amaranthus hypochondriacus, has a well-balanced amino acid composition and high levels of essential amino acids and offers the possibility of further improving food animal feed additives. In order to find an effective means of expressing AmA1 in yeast, the gene was cloned into an episomal shuttle vector. Four different promoters were tested: the glyceraldehyde-3-phosphate dehydrogenase promoter, galactose dehydrogenase 10 promoter, alcohol dehydrogenase II promoter, and a hybrid ADH2-GPD promoter. The recombinant AmA1 genes were then introduced into the yeast Saccharomyces cerevisiae 2805. Northern and Western blot analyses of the yeast under appropriate conditions revealed that AmA1 was expressed by all four promoters at varying levels. An enzyme-linked immunosorbent assay demonstrated that the amount of AmA1 protein in the recombinant yeast was 1.3-4.3% of the total soluble proteins. The highest expression level was obtained from the hybrid ADH2-GPD promoter.

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Construction of a Transposon-mediated Baculovirus Vector Hanpvid and a New Cell Line for Expressing Barnase

  • Qin, Qin;Liu, Ying-Le;Zhu, Ying;Li, Shun-Yi;Qi, Yi-Peng
    • BMB Reports
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    • v.38 no.1
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    • pp.41-48
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    • 2005
  • In this study we developed the transposon-mediated shuttle vector 'Hanpvid', which composed of HaNPV (Heliothis armigera nuclear polyhedrosis virus) genomic DNA and a transposon cassette from Bacmid of Bac-to-Bac system. Hanpvid replicates in E. coli in the same way as Bacmid and retains infective function in cotton bollworm cells (Hz-AM1). Using Hanpvid we constructed a recombinant virus, which could infect Hz-AM1 cells and generate recombinant HaNPV (rHa-Bar) containing the barnase gene, a ribonuclease gene from Bacillus amyloliquefaciens. Since the expression vector carrying barnase gene cannot replicate in the absence of barstar, a specific inhibitor of barnase, we constructed a new cotton bollworm cell line (AM1-NB) using the marker rescue method. In AM1-NB barstar was integrated into the cellular chromosome to sustain the replication of rHa-Bar. To screen out recombinant HaNPV for potential use as biopesticide, Hz-AM1 and AM1-NB cell lines were infected with rHa-Bar, respectively. The results obtained indicate that Viral progenies in AM1-NB were 23 and 160 times greater than those in Hz-AM1 48 h and 72 h after infection, respectively. With additional insertion of the polyhedron gene from AcNPV (Autographa californica nuclear polyhedrosis virus) into the Hanpvid genome, rHa-Bar regained the polyhedron phenotype and its pest-killing rate greatly improved. Toxic analysis showed that the lethal dosages ($LD_{50}$) and the lethal time(s) ($LT_{50}$) of rHa-Bar were reduced by 20% and 30%, respectively, compared to wt-HaNPV in the third instar larvae of cotton bollworm. This study shows that in AM1-NB barnase can be effectively produced and used as pest-killing agent for the biological control of cotton pests.

Radioanalytical and Spectroscopic Characterizations of Hydroxo- and Oxalato-Am(III) Complexes (방사분석과 분광학을 이용한 Am(III) 가수분해와 옥살레이트 착물 화학종 연구)

  • Kim, Hee-Kyung;Cho, Hye-Ryun;Jung, Euo Chang;Cha, Wansik
    • Journal of Nuclear Fuel Cycle and Waste Technology(JNFCWT)
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    • v.16 no.4
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    • pp.397-410
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    • 2018
  • When considering the long-term safety assessment of spent-nuclear fuel management, americium is one of the most radio-toxic actinides. Although spectroscopic methods are widely used for the study of actinide chemistry, application of those methods to americium chemistry has been limited. Herein, we purified $^{241}Am$ to obtain a highly pure stock solution required for spectroscopic studies. Quantitative and qualitative analyses of purified $^{241}Am$ were carried out using liquid scintillation counting, and gamma and alpha radiation spectrometry. Highly sensitive absorption spectrometry coupled with a liquid waveguide capillary cell and time-resolved laser fluorescence spectroscopy were employed for the study of Am(III) hydrolysis and oxalate (Ox) complexation. $Am^{3+}$ ions under acidic conditions exhibit maximum absorbance at 503 nm, with a molar absorption coefficient of $424{\pm}8cm^{-1}{\cdot}M^{-1}$. $Am(OH)_3(s)$ colloidal particles formed under near neutral pH conditions were identified by monitoring the absorbance at around 506-507 nm. The formation of ${Am(Ox)_3}^{3-}$ was detected by red-shifts of the absorption and luminescence spectra of 4 and 5 nm, respectively. In addition, considerable enhancements of the luminescence intensities were observed. The luminescence lifetime of ${Am(Ox)_3}^{3-}$ increased from 23 to 56 ns, which indicates that approximately six water molecules are replaced by carboxylate ligands in the inner-sphere of the Am(III). These results suggest that ${Am(Ox)_3}^{3-}$ is formed through the bidentate coordination of the oxalate ligands.