• 제목/요약/키워드: Alu

검색결과 205건 처리시간 0.022초

Sapphire 결정성장 (Crystal Growth of Sapphire)

  • 최종건;오근호
    • 한국세라믹학회지
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    • 제23권1호
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    • pp.21-26
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    • 1986
  • By the floating zone method with infrared radiation convergence type heater homogeneously $Cr^{3+}$ doped alu-mina single crystal was obtained. And sizx {1010} facets appeared at the surface of [0001] grown crystals. $ZrO_2$ and $HfO_2$ precipitated as secondary phase and were not doped in the crystals. We found that the dist-ribution of the secondary phase which was mainly located at the surface and the peripheral region was closely related to the flow pattern of melt zone.

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단순 전력분석 공격에 대처하는 타원곡선 암호프로세서의 하드웨어 설계 (Hardware Design of Elliptic Curve processor Resistant against Simple Power Analysis Attack)

  • 최병윤
    • 한국정보통신학회논문지
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    • 제16권1호
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    • pp.143-152
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    • 2012
  • 본 논문은 스칼라 곱셈, Menezes-Vanstone 타원곡선 암호 및 복호 알고리즘, 점-덧셈, 점-2배 연산, 유한체상 곱셈, 나눗셈 등의 7가지 동작을 수행하는 GF($2^{191}$) 타원곡선 암호프로세서를 하드웨어로 설계하였다. 단순 전력 분석에 대비하기 위해 타원곡선 암호프로세서는 주된 반복 동작이 키 값에 무관하게 동일한 연산 동작으로 구성되는 몽고메리 스칼라 곱셈 기법을 사용하며, GF($2^m$)의 유한체에서 각각 1, (m/8), (m-1)개의 고정된 사이클에 완료되는 GF-ALU, GF-MUL, GF-DIV 연산장치가 병렬적으로 수행되는 동작 특성을 갖는다. 설계된 프로세서는 0.35um CMOS 공정에서 약 68,000개의 게이트로 구성되며, 시뮬레이션을 통한 최악 지연시간은 7.8 ns로 약 125 MHz의 동작속도를 갖는다. 설계된 프로세서는 320 kps의 암호율, 640 kbps을 복호율 갖고 7개의 유한체 연산을 지원하므로 다양한 암호와 통신 분야에 적용할 수 있다.

DNA Polymorphism in SLC11A1 Gene and its Association with Brucellosis Resistance in Indian Zebu (Bos indicus) and Crossbred (Bos indicus×Bos taurus) Cattle

  • Kumar, Nishant;Ganguly, Indrajit;Singh, Rajendra;Deb, Sitangsu M.;Kumar, Subodh;Sharma, Arjava;Mitra, Abhijit
    • Asian-Australasian Journal of Animal Sciences
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    • 제24권7호
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    • pp.898-904
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    • 2011
  • The PCR- restriction fragment length polymorphism (RFLP) in and around TM4 of SLC11A1 gene and its association with the incidences of brucellosis in Hariana breed (Bos indicus) and Holstein Friesian crossbred (Bos indicus${\times}$Bos taurus) cattle was examined. A fragment of 954 bp encoding the TM4 was amplified, and RFLP was identified by digestion of the amplicon independently with AluI and TaqI. The amplicon (GenBank Acc. No. AY338470 and AY338471) comprised of a part of exon V (<59 bp) and VII (62>), and entire intron 5 (423 bp), exon VI (71 bp) and intron 6 (339 bp). Digestion with AluI revealed the presence of two alleles viz, A (281, 255, 79 and 51 bp) and B (541, 255, 79 and 51 bp). The frequency of A allele was estimated as 0.80 and 0.73 in Hariana and crossbred cattle, respectively. Due to presence of a polymorphic TaqI site at intron 5, two alleles: T (552 and 402 bp) and Q (231, 321 and 402 bp) were identified. The frequency of T allele was estimated as 0.96 and 0.97, respectively. For association study, on the basis of serological tests and history of abortion, the animals were grouped into "affected" and "non-affected". However, no association could be established with the observed RFLPs.

PCR-Based RELP Analysis of ureC Gene for Typing of Indian Helicobacter pylori Strains from Gastric Biopsy Specimens and Culture

  • Mishra, Kanchan-Kumar;Prabhat P. Dwivedi;Prasad, Kashi-Nath;Archana Ayyagari
    • Journal of Microbiology
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    • 제40권4호
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    • pp.282-288
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    • 2002
  • Since culture of Helicobacter pylori is relatively insensitive and cumbersome, molecular detection and typing of H. pylori isolates are gaining importance for strain differentiation. In the present study genomic DNA of 42 gastric biopsies and H. pylori isolates from corresponding patients were analyzed and compared by PCR-based RFLP assay. The 1,132-bp product representing an internal portion of ureC gene of H. pylori was amplified by PCR and digested with restriction enzymes HindⅢ, AiuⅠ and PvuⅠ. The HindⅢ, AluⅠ and PvuⅠ digestion produced 4, 7, and 2 distinguishable RFLP patterns respectively from 42-H. pylori isolates. By combining all three restriction enzyme digestions, 15 RFLP patterns were observed. However, when PCR products from 42 gastric biopsy specimens were digested by restriction enzymes HindⅢ, AluⅠ and PvuⅠ, we observed 5, 8 and 2 RFLP patterns, respectively. Patterns from 34 of 42 gastric biopsy specimens matched those of corresponding H. pylori isolates from respective patients. Patterns from the remaining eight biopsy specimens differed and appeared to represent infection with two H. pylori strains. The patterns of one strain from each of these biopsies was identical to that of the isolate from corresponding patients and the second pattern presumably represented the co-infecting strain. From the study, it appears that PCR-based RFLP analysis is a useful primary tool to detect and is distinguish H. pylori strains from gastric biopsy specimens and is superior to culture techniques in the diagnosis of infection with multiple strains of H. pylori.

두릅나무 묘목생산포의 역병 발생 및 분리균의 균학적 특성 (Root Rot of Japanese Angelica Caused by Phytophthora cactorum in Nursery and Mycological Characteristics of the Isolates)

  • 이상현;이재필;김경희;신현동
    • 한국균학회지
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    • 제33권2호
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    • pp.98-102
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    • 2005
  • 2003년부터 2005년 사이에 한국의 두릅나무에 심각한 역병이 발생하였다. 감염된 두릅나무와 토양으로부터 병원균이 분리되었으며, 이 균은 배양적인 그리고 형태적인 특징 및 병원성 검증 실험에 의해 P. cactorum(Lebert&Cohn) J. Schrt.으로 동정되었다. 이 균은 뚜렷한 돌출형이고, 둥근 난형모양의 탈락성 유주포자낭의 특정으로 다른 역병균으로부터 형태적으로 구분되었다. 최적의 생장 온도는 V8 배지와 Oat meal 배지에서의 $25^{\circ}C$이었다. PCR을 통해 ITS rDNA 영역의 약 900 bp의 길이가 증폭되었으며, 세 가지 효소인 Alu I, Msp I, Taq I을 이용한 PCR-RFLP 분석 결과를 PhytID 의 데이터베이스에서 분석한 결과 형태적인 그리고 배양적인 특징에 의한 결과와 일치했다.

국내에 존재하는 세 종류 메타고니무스속 흡충의 RCR-RFLP반응양상 (PCR-RFLP patterns of three kinds of Metagonimus in Korea)

  • 유재란;정진성
    • Parasites, Hosts and Diseases
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    • 제35권4호
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    • pp.271-276
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    • 1997
  • 메타고니무스속 흡충의 형태학적인 차이점은 잘 알려져 있으나 이러한 미세한 형태학적 차이로 종을 분류할 수 있을 지에 대해서는 의문시되어 왔다. 이 연구는 비교적 유전자 염기서열이 잘 보존되어 있 어 종간 또는 strain간의 차이를 밝힐 수 있는 리보솜리보핵산 유전자 중 ITSI 유전자와 사립체 COI 유전자를 중합효소반응으로 증폭시킨 후 제한효소로 소화시켜 나타나는 밴드의 차이를 관찰하였다 요 코가와흡충 (M. yokogawai)의 피 낭유충은 삼척산 은어에서 , 미야타흡충 (Metagonim Miyata type) 은 충주산 피라미에서, 타카하시홉충 (M. tnkqhqsrii)은 충주산 붕어에서 분리하여 사용하였다. 세 종류 충체에서 얻은 ml 유전자 증폭산물은 제한효소 Rsc I, Ak I 및 Msp I에 의해 서로 다른 크기의 밴드 로 소화되었다. 세 종류 충체의 사립체 COI 유전자 증폭산물도 Rsc I과 AIu I에 의해 서로 다른 양상으로 잘라졌다. 추정 유전자 차이 (estimated genetic divergence)는 미야타홉충과 요코가와흡충이 0.034880, 요코가와흡충과 타카하시홉충이 0.018179, 미야타흡충과 타카하시흡충이 0.028098 이었다. 이 결과로 보면 미야타흡충은 별개의 종으로 볼 수 있으며,다른 충체보다 이른 시기에 진화하였음 을 알 수 있다.

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Tracking Intravenous Adipose-Derived Mesenchymal Stem Cells in a Model of Elastase-Induced Emphysema

  • Kim, You-Sun;Kim, Ji-Young;Shin, Dong-Myung;Huh, Jin Won;Lee, Sei Won;Oh, Yeon-Mok
    • Tuberculosis and Respiratory Diseases
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    • 제77권3호
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    • pp.116-123
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    • 2014
  • Background: Mesenchymal stem cells (MSCs) obtained from bone marrow or adipose tissue can successfully repair emphysematous animal lungs, which is a characteristic of chronic obstructive pulmonary disease. Here, we describe the cellular distribution of MSCs that were intravenously injected into mice with elastase-induced emphysema. The distributions were also compared to the distributions in control mice without emphysema. Methods: We used fluorescence optical imaging with quantum dots (QDs) to track intravenously injected MSCs. In addition, we used a human Alu sequence-based real-time polymerase chain reaction method to assess the lungs, liver, kidney, and spleen in mice with elastase-induced emphysema and control mice at 1, 4, 24, 72, and 168 hours after MSCs injection. Results: The injected MSCs were detected with QD fluorescence at 1- and 4-hour postinjection, and the human Alu sequence was detected at 1-, 4- and 24-hour postinjection in control mice (lungs only). Injected MSCs remained more in mice with elastase-induced emphysema at 1, 4, and 24 hours after MSCs injection than the control lungs without emphysema. Conclusion: In conclusion, our results show that injected MSCs were observed at 1 and 4 hours post injection and more MSCs remain in lungs with emphysema.