• Journal of Radiation Protection and Research
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• v.37 no.4
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• pp.213-218
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• 2012

Alpha spectrometry has been typically used for determination of the uranium isotopes in soil. For a number of uranium analysis in soil samples, rapid sample digestion with limited quantities of mixed acid containing HF will give a contribution for effective management of uranium analysis. Microwave digestion system is evaluated for rapid sample digestion using reference uranium soil (IAEA-375 soil). For completion of 0.5 g of soil digestion by microwave, 3 ml of HF in a 10 ml of mixed acid is minimum requirement volume for completed soil digestion for 80 minutes. Microwave digestion is timely effective techniques for uranium measurement using alpha spectrometry compared to the other methods (open vessel digestion, closed vessel digestion) due to rapid sample digestion. In addition, it can be reduced the occurrence of hazardous substances by minimizing the amount of HF.

• Nuclear Engineering and Technology
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• v.39 no.5
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• pp.673-682
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• 2007

The contents of transuranic elements in high-burnup spent fuel samples were determined. The activity amounts of $^{238}Pu,\;^{239}Pu,\;^{240}Pu,\;^{241}Am,\;^{244}Cm\;and\;^{242}Cm$ were measured by alpha spectrometry using $^{242}Pu\;and\;^{243}Am$ as tracers, respectively. A spike addition method for $^{237}Np$ was established by an alpha and gamma spectrometry using $^{239}Np$ as a spike after the optimum conditions for the measurements of $^{237}Np\;and\;^{239}Np$, respectively, were obtained. A separation system using anion exchange chromatography and diethylhexylphosphoric acid extraction chromatography was applied for the separation of these elements. This method was applied to high-burnup spent nuclear fuel samples $(40{\sim}60GWD/MTU)$. The contents of the transuranic elements were compared with those by ORIGEN-2 code. Measurements and the calculations of the contents of the plutonium isotopes $^{238}Pu,\;^{239}Pu\;and\;^{240}Pu$ agreed to within 10% on average. The contents of $^{237}Np$ agreed to within approximately 5% except for one instance of a calculation, while those of $^{241}Am,\;^{244}Cm\;and\;^{242}Cm$ showed higher values by approximately 19%, 35% and 14% on average, respectively, compared to the calculations according to the burnup.

• Molecules and Cells
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• v.38 no.1
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• pp.65-74
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• 2015

Carbohydrate antigens expressed on pig cells are considered to be major barriers in pig-to-human xenotransplantation. Even after ${\alpha}1,3$-galactosyltransferase gene knock-out (GalT-KO) pigs are generated, potential non-Gal antigens are still existed. However, to the best of our knowledge there is no extensive study analyzing N-glycans expressed on the GalT-KO pig tissues or cells. Here, we identified and quantified totally 47 N-glycans from wild-type (WT) and GalT-KO pig fibroblasts using mass spectrometry. First, our results confirmed the absence of galactose-alpha-1,3-galactose (${\alpha}$-Gal) residue in the GalT-KO pig cells. Interestingly, we showed that the level of overall fucosylated N-glycans from GalT-KO pig fibroblasts is much higher than from WT pig fibroblasts. Moreover, the relative quantity of the N-glycolylneuraminic acid (NeuGc) antigen is slightly higher in the GalT-KO pigs. Thus, this study will contribute to a better understanding of cellular glycan alterations on GalT-KO pigs for successful xenotransplantation.

• Mass Spectrometry Letters
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• v.5 no.2
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• pp.49-51
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• 2014

The effectiveness of tertiary matrices composed of the combination of three common matrices (dihydrobenzoic acid (DHB), ${\alpha}$-cyano-4-hydroxycinnamic acid (CHCA), and sinapinic acid (SA)) was compared with that of single or binary matrices in the analysis of polyethylene glycol (PEG) polymers ranging from 1400 to 10000 Da using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS). A tertiary matrix of 2,5-DHB+CHCA+SA was the most effective in terms of S/N ratios. CHCA and CHCA+SA produced the highest S/N ratios among the single matrices and the binary matrices, respectively. The improvement observed when using a tertiary matrix in analyses of PEG polymers by MALDI-TOF MS is believed to be due to the uniform morphology of the MALDI sample spots and synergistic effects arising from the mixture of the three matrix materials.

• Nuclear Engineering and Technology
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• v.50 no.1
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• pp.159-164
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• 2018

An evaluation of various radiometric methods to analyze $^{226}Ra$ in water has been employed on a set of 10 standard solutions of different concentrations in the range of $1-10Bq/L^{-1}$. The analysis was carried out using well-established procedures by means of gamma-ray, alpha-particle and liquid scintillation spectrometry. The feasibility of the various methods has been quantified in terms of relative standard error and percentage error. Correlations between the various methods have been presented and discussed. In general, good agreement was found in the results of various methodologies, which assures the accuracy of the methods and allows for the validation of instrumentation and procedures. Of the different methods adopted here, a combined procedure for the determination of $^{226}Ra$ along with $^{228}Ra$ using Quantulus 1220 ultra-low level background liquid scintillation counting gave the most accurate results.

• Korean Journal of Food Science and Technology
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• v.20 no.6
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• pp.769-773
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• 1988

The needle oils of Pious rigida Mill and Pious densiflora Sieb & Zucc were analyzed by gas chromatography/mass spectrometry. The major components of Pinus rigida were ${\alpha}-pinene$, 1-hexen-3-ol formate, sabinene, ${\beta}-pinene,\;{\alpha}-terpineol$ and ${\beta}-caryophyllene,\;{\alpha}-pinene$, bornyl acetate, ${\beta}-pinene$ and ${\beta}-hpellandrene$ were the major components fo Pious densiflora. Pious densiflora had sweeter and more greenish note than Pines rigida because the bornyl acetate content of Pious densiflora was about three times more than that of Pious rigida.

• Nuclear Engineering and Technology
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• v.41 no.1
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• pp.99-106
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• 2009

The contents of transuranic elements ($^{237}Np$, $^{238}Pu$, $^{239}Pu$, $^{240}Pu$, $^{241}Am$, $^{244}Cm$, and $^{242}Cm$) in high-burnup spent fuel samples ($35.6{\sim}53.9\;GWd/MtU$) were determined by alpha spectrometry. Anion exchange chromatography and diethylhexyl phosphoric acid extraction chromatography were applied for the separation of these elements from the uranium matrix. The measured values of the nuclides were compared with ORIGEN-2 calculations. For plutonium, the measurements were higher than the calculations by about $2.6{\sim}32.7%$ on average according to each isotope, and those for americium and curium were also higher by about $35.9{\sim}63.1%$. However, for $^{237}Np$, the measurements were lower by about 52% on average for the samples.

• Preventive Nutrition and Food Science
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• v.21 no.2
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• pp.124-131
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• 2016

Among 116 bacterial strains isolated from Korean fermented foods, one strain (SS-76) was selected for producing new oligosaccharides in a basal medium containing maltose as the sole source of carbon. Upon morphological characterization using scanning electron microscopy, the cells of strain SS-76 appeared rod-shaped; subsequent 16S rRNA gene sequence analysis revealed that strain SS-76 was phylogenetically close to Bacillus subtilis. The main oligosaccharide fraction B extracted from the culture supernatant of B. subtilis SS-76 was purified by high performance liquid chromatography. Subsequent structural analysis revealed that this oligosaccharide consisted only of glucose, and methylation analysis indicated similar proportions of glucopyranosides in the 6-linkage, 4-linkage, and non-reducing terminal positions. Matrix-assisted laser-induced/ionization time-of-flight/mass spectrometry and electrospray ionization-based liquid chromatography-mass spectrometry/mass spectrometry analyses suggested that this oligosaccharide consisted of a trisaccharide unit with 1,6- and 1,4-glycosidic linkages. The anomeric signals in the $^1H$-nuclear magnetic resonance spectrum corresponded to ${\alpha}$-anomeric configurations, and the trisaccharide was finally identified as panose (${\alpha}$-D-glucopyranosyl-1,6-${\alpha}$-D-glucopyranosyl-1,4-D-glucose). These results suggest that B. subtilis SS-76 converts maltose into panose; strain SS-76 may thus find industrial application in the production of panose.

• Analytical Science and Technology
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• v.17 no.1
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• pp.85-89
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• 2004

Fragrance components of lilac (Syringa vulgaris) blossom have been characterized in this paper. The accurate characterization of fragrances collected from lilac blossom was carried out by solid-phase trapping-solvent extraction and gas chromatography-ion trap mass spectrometry. According to lilac species, the chemical compositions were significantly different. Benzaldehyde, phenylacetaldehyde, and ${\alpha}$-farnesene were found as the predominant component of white lilac blossom whereas benzaldehyde, ${\alpha}$-pinene, and ocimene were those of pale purple lilac. The enantiomeric analysis of ${\alpha}$-pinene in lilac blossom was found in the form of ( ).

• Korean Journal of Food Science and Technology
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• v.20 no.6
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• pp.774-779
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• 1988

The essential oil of Artemiisa lavandulaefolia DC was analyzed by gas chromatography/mass spectrometry (GC/MS). The oil was stored at different conditions for 6 weeks and the changes of color and chemical composition during storage were checked by GC/MS. The experimental results were as follows; (1) More than 186 chemicals were detected by GC. Major components were 1,8-cineol, thujone, camphor, borneol, coumarin, 2,3-dihydrobenzofuran and ${\beta}-biasbolene$. In this study, 3,3,6-trimethyl norpinanol, ${\beta}-farnesene,\;{\alpha}-curmene$ and 7-methoxy coumarin were detected as new compounds in Artemisia species. (2) It was proved that temperature and/ or light had and important effect on the changes of color and volatile components of the essential oil. The relative amounts of limonene and 1.8-cineol were decreasad, on the other hand, ${\beta}-phellandrene\;and\;{\alpha}-terpineol$ were increased during storage.