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http://dx.doi.org/10.14348/molcells.2015.2240

Comparative N-Linked Glycan Analysis of Wild-Type and α1,3-Galactosyltransferase Gene Knock-Out Pig Fibroblasts Using Mass Spectrometry Approaches  

Park, Hae-Min (School of Chemical and Biological Engineering, Seoul National University)
Kim, Yoon-Woo (Department of Chemical Engineering, Soongsil University)
Kim, Kyoung-Jin (Department of Chemical Engineering, Soongsil University)
Kim, Young June (Department of Nanobiomedical Science and BK21+ NBM Global Research Center for Regenerative Medicine, Dankook University)
Yang, Yung-Hun (Department of Microbial Engineering, College of Engineering, Konkuk University)
Jin, Jang Mi (Division of Mass Spectrometry Research, Korea Basic Science Institute)
Kim, Young Hwan (Division of Mass Spectrometry Research, Korea Basic Science Institute)
Kim, Byung-Gee (School of Chemical and Biological Engineering, Seoul National University)
Shim, Hosup (Department of Nanobiomedical Science and BK21+ NBM Global Research Center for Regenerative Medicine, Dankook University)
Kim, Yun-Gon (Department of Chemical Engineering, Soongsil University)
Publication Information
Molecules and Cells / v.38, no.1, 2015 , pp. 65-74 More about this Journal
Abstract
Carbohydrate antigens expressed on pig cells are considered to be major barriers in pig-to-human xenotransplantation. Even after ${\alpha}1,3$-galactosyltransferase gene knock-out (GalT-KO) pigs are generated, potential non-Gal antigens are still existed. However, to the best of our knowledge there is no extensive study analyzing N-glycans expressed on the GalT-KO pig tissues or cells. Here, we identified and quantified totally 47 N-glycans from wild-type (WT) and GalT-KO pig fibroblasts using mass spectrometry. First, our results confirmed the absence of galactose-alpha-1,3-galactose (${\alpha}$-Gal) residue in the GalT-KO pig cells. Interestingly, we showed that the level of overall fucosylated N-glycans from GalT-KO pig fibroblasts is much higher than from WT pig fibroblasts. Moreover, the relative quantity of the N-glycolylneuraminic acid (NeuGc) antigen is slightly higher in the GalT-KO pigs. Thus, this study will contribute to a better understanding of cellular glycan alterations on GalT-KO pigs for successful xenotransplantation.
Keywords
GalT-KO pig fibroblast; mass spectrometry (MS); N-glycan; N-glycolylneuraminic acid (NeuGc);
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