• 분석과학
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• 제5권2호
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• pp.191-197
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• 1992

익산군(益山郡) 용제리(龍提里)에서 출토(出土)된 기원전(紀元前)의 세형동검(細形銅劍)을 습식(濕式) 및 원자흡수분광법(原子吸收分光法)으로 조성(組成)을 정량(定量)하였으며, 시편(試片)을 연마(硏磨), 부식(腐蝕)시켜 광학현미경(光學顯微鏡)과 주사형(走査型) 전자현미경(電子顯微鏡)으로 미세구조(微細構造)를 관찰(觀察)하였다. 또한 질량분석계(質量分析計)를 써서 납동위원소비(同位元素比)를 측정(測定)하여 원료(原料)의 산지(産地)를 추정(推定)코자 하였다. 세형동검(細形銅劍)은 Cu-Sn-Pb계(系)로서 Sn이 17%, Pb가 6.8%로서 Sn이 10% 이상이고 Pb가 첨가(添加)된 것으로 보아 청동기(靑銅器) 중기(中期) 이후(以後)임을 알 수 있었다. 시료내부(試料內部)는 ${\alpha}$와 (${\alpha}+{\delta}$) 공정(共晶)으로 되어 있으며 편석(偏析)이 일어나 있었다. 원료(原料)는 중국(中國) 북부산(北部産)일 가능성(可能性)이 크다.

• Archives of Pharmacal Research
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• 제20권3호
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• pp.247-252
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• 1997

Perfluorotolyl (PFT)-ether and perfluorotoly-trimethylsilyl (PFT-TMS) ether derivatives of oestrone, $17{\alpha}$- and $17{\beta}$oestradiol were prepared under phase transfer conditons. The former derivatives under negative ion chemical ionization conditions gave significant ions in the mass spectrometer but $17{\alpha}$- and $17{\beta}$ -oestradiol gave poor resolution. However, the PFT-TMS derivatives of 17.${\alpha}$- and$17{\beta}$-oestradiol showed good resolution. These derivatives were used for the analysis of oestrogens in bovine aqueous humour, vitreous humour and retina. The mean $({\pm}SEM)$ concentrations of oestrone in bovine aqueous humour (n=18), vitreous humour (n=18) and bovine retina (n=4) were $0.47{\pm}0.11$, $0.46{\pm}0.14$ and $1.10{\pm}0.24 ng.ml^{-1}$, respectively. $17{\alpha}$-Oestradiol was detected in 16 out of 18 samples of bovine aqueous humour and vitreous humour and the mean $({\pm}SEM)$ concentrations were $0.30{\pm}0.10$ and $0.08{\pm}0.02 ng.ml^{-1}$, respectively. The mean $({\pm}SEM)$ concentration of 17.betha.-oestradiol in aqueous humour (n=7) and vitreous homour (n=11) $0.83{\pm}0.26 ng ml^{-1}$ and $0.39{\pm}0.09 ng ml^-1$, respectively. In retina the concentrations of both steroids were below the detection limit.

• 생약학회지
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• 제46권2호
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• pp.105-108
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• 2015

To establish the anti-diabetic(α-glucosidase inhibitory) activity of D. lotus leaf extract, isolate and identify the constituents responsible for the activity. The methanolic extract of leaves was partitioned between water, n-butanol and ethyl acetate. Bio-assay guided fractionation, based on inhibition of ;${\alpha}$-glucosidase, allowed isolation and identification of the active components. Liquid chromatography/mass spectrometry(LC/MS), ¹ H-NMR and ¹³ C-NMR spectra analyses demonstrated that the active compound was myricetin-3-O-;${\alpha}$-L-rhamnoside(1). Compound 1 demonstrated a strong inhibition on the α-glucosidase, in vitro and ;${\alpha}$-glucosidase inhibitory value was calculated as 98.08%, when that of a reference drug, acarbose was estimated as 83.03%. The present study indicates compound 1 could be considered as an ;${\alpha}$-glucosidase inhibitor and developed as an important antidiabetes agent for type II diabetes therapy.

• Asian Pacific Journal of Cancer Prevention
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• 제14권4호
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• pp.2273-2276
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• 2013

Objectives: This study employed proteomic profiling to identify specific tumor markers that might improve early diagnosis of lung squamous cell carcinoma. Methods: Serum samples were isolated from 30 patients with stage I lung squamous cell carcinoma and 30 age-and gender-matched healthy controls, and proteomic profiles were obtained by matrix-assisted laser desorption ionization time of flight mass spectrometry. Results: Three highly expressed potential tumor markers were identified in the sera of stage I lung squamous cell carcinoma patients, with molecular weights of 3261.69, 3192.07, and 2556.92 Da. One protein peak with molecular weight 3261.69 Da was chosen as the candidate biomarker and identified as a fibrinogen alpha chain through a search of the IPI, NCBI or SWISS-PROT protein databases. Conclusion: As a potential tumor biomarker, fibrinogen alpha chain may be applicable for the early diagnosis and prognosis of lung squamous cell carcinoma patients.

• BMB Reports
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• 제39권3호
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• pp.311-318
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• 2006

One of the biggest group of proteins influenced by protein kinase CK2 is formed by factors engaged in gene expression. Here we have reported recently identified yeast transcription elongation factor Elf1 as a new substrate for both monomeric and tetrameric forms of CK2. Elf1 serves as a substrate for both the recombinant CK2$\alpha$' ($K_m$ 0.38 ${\mu}M$) and holoenzyme ($K_m$ $0.13\;{\mu}M$). By MALDI-MS we identified the two serine residues at positions 95 and 117 as the most probable in vitro phosphorylation sites. Co-immunoprecypitation experiments show that Elf1 interacts with catalytic ($\alpha$ and $\alpha$') as well as regulatory ($\beta$ and $\beta$') subunits of CK2. These data may help to elucidate the role of protein kinase CK2 and Elf1 in the regulation of transcription elongation.

• Applied Biological Chemistry
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• 제27권4호
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• pp.259-263
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• 1984

수삼(水蔘)으로부터 얻은 방향성(芳香性) 화합물(化合物)을 hexane으로 추출(抽出)한 분획(分劃)을 모세관 칼럼 크로마토그라피법으로 분리(分離)한 결과(結果) 약 50까지의 성분(成分)이 분리(分離)되었으며 이중에서 GC/MS에 의해서 26가지를 일차적(一次的)으로 동정(同定)할 수 있었다. 이들 중에는 ester와 ether가 절반가량 되었으며 나머지는 3가지의 monoterpene과 7가지의 sesquiterpene, 2가지의 sesquiterpenol, 한가지의 sesquiterpenediol이 동정(同定)되었다. 보고(報告)된 Kovat지수(指數)와 비교해 본 결과 ${\alpha}-guaine$만이 일치(一致)하였다. 인삼(人蔘)의 특유(特有)한 향기성분(香氣成分)은 이들 sesquiterpenoid중에서 몇 가지 성분일 것으로 추측된다.

• Journal of Applied Biological Chemistry
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• 제50권3호
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• pp.164-169
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• 2007

Three coniferous essential oils were extracted from Abies koreana, Cryptomeria japonica, and Torreya nucifera by hydrodistillation. The chemical composition of each oil was analyzed by GCMS, and their antimicrobial activities were tested against two bacteria and one yeast strains. Fortyseven compounds were identified from A. koreana oil, 39 from C. japonica, and 59 from T. nucifera. Main components of the essential oils were limonene (23.5%), bornyl acetate (17.9%), ${\alpha}-pinene$ (11.1%), and camphene (10.2%) in A. koreana, kaurene (26.3%), ${\gamma}-eudesmol$ (19.0%), elemol (6.9%), and sabinene (5.1%) in C. japonica, limonene (13.5%), ${\delta}-cadinene$ (10.5%), ${\alpha}-bisabolol$ (10.2%), and ${\alpha}-copaene$ (7.7%) in T. nucifera. Among the three coniferous trees tested, the essential oils of A. koreana exhibited higher and broader antimicrobial activity against the tested organisms than those of C. japonica and T. nucifera.

• Preventive Nutrition and Food Science
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• 제7권1호
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• pp.5-11
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• 2002

The comparison of the volatile flavor components from Korean and Japanese Satsuma mandarin (C. unshiu Marcov. forma Miyagawa-wase) peel oils, isolated by cold-pressing, was performed by gas chromatography, mass-spectrometry and gas chromatography-olfactometry (GC-O). Eighty-five volatile components were identified in each oil by GC and GC-MS. Forty-three components were detected in each oil by GC-O. The total amount of monoterpene hydrocarbons was 95.88% (Korean mandarin) and 95.29% (Japanese mandarin). Limonene, ${\gamma}$-terpinene, myrcene and $\alpha$-pinene were the main components of the cold-pressed oils from the both samples. The volatile composition of the Japanese mandarin was characterized by a higher content of sesquiterpene hydrocarbons, especially bicyclogermacrene, $\alpha$-humullene and valencene. The volatile composition of two samples can easily be distinguished by the percentages of aldehydes, ketones and esters, which were found at higher levels in the Japanese mandarin. The sweet and fruity flavor was stronger in the Korean mandarin oil while herbaceous flavor was stronger in Japanese sample. From GC-O data it is suggested that the sweet and fruity flavor of the Korean mandarin resulted from terpinolene and linalool, and the herbaceous note of the Japanese mandarin from $\alpha$-humullene, nepal, ι-carvone and perill aldehyde.

• 한국잡초학회지
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• 제31권2호
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• pp.146-151
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• 2011

메타세콰이아(Metasequoia glyptostroboides)의 구과로부터 수증기증류법을 이용하여 정유를 추출하였고, 정유의 향취를 분석하였으며 이에 함유되어 있는 휘발성 화학성분을 solid-phase microextraction 장치가 장착된 gas chromatography-mass spectrometry를 이용하여 분석하였다. 메타세콰이아의 구과에 함유된 정유는 0.4%이었다. 정유의 주향취는 woody, coniferous, herbal이었으며, 부향취는 minty, spicy, oily이었다. 정유에는 총 9종의 휘발성 유기화합물이 함유되어 있었는데 이들을 화학성분별로 구분하면 탄화수소가 8종 그리고 옥사이드가 1종이었다. 정유에 함유된 휘발성 유기화합물은 limonene(66.18%), ${\delta}$-3-carene(11.11%), ${\beta}$-caryophyllene(6.66%), ${\beta}$-myrcene(5.92%), ${\beta}$-pinene(4.14%), caryophyllene oxide (2.39%), camphene(2.32%), ${\alpha}$-caryophyllene(0.85%), tricyclene(0.43%)이었다. 메타세콰이아 구과로부터얻은 정유의 herbal과 minty 향취는 limonene과 ${\delta}$-3-carene, spicy 향취는 caryophyllene, coniferous 향취는 ${\alpha}$-pinene과 ${\beta}$-pinene, oily 향취는 camphene에 의해 발현된 것이라 판단된다.

• 분석과학
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• 제13권5호
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• pp.601-607
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• 2000

란탄은 사용후핵연료의 연소도 지표원소들 중 하나로써 이용되고 있다. $U_3Si/Al$ 사용후핵연료는 다량의 U과 Al 속에 미량의 La이 포함되어 있어 정량시 매질의 영향을 줄이기 위해 화학적 분리가 요구된다. La의 분리 및 측정을 위해 IC-ICP-MS를 이용하였으며, 우선 방사성 시료를 취급하기 위하여 유도결합 플라스마 질량분석기의 플라스마 부분 및 분리관을 방사선 차폐 글로브박스 내에 설치하였다. CG10 분리관과 ${\alpha}$-HiBA 용리액을 사용하여 U, Al, La 및 몇 가지 핵분열생성물 (Sr, Zr, Y, Mo, Ru, Pd, Rh, Cs, Ba, Ce, Pr, Nd, Sm, Eu 및 Cd)의 머무름 거동을 살펴보았다. 0.2 M ${\alpha}$-HiBA 용리액에서 U과 Al이 초기에 용출되므로 분리관과 ICP-MS의 시료분무기 사이에 3방향 밸브를 연결하여 다량의 U과 Al이 ICP-MS로 유입되지 않도록 하므로써 매질의 영향을 줄일 수 있었다. 이 조건에서 La은 약 12분 정도에 분리 및 측정이 가능하였으며, $1-10{\mu}g/L$ (ppb)의 농도범위가 측청에 적합하였고 시료양을 $200{\mu}L$ 취할 경우 La의 검출한계는 $0.25{\mu}g/L$이었다.