• Asian Pacific Journal of Cancer Prevention
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• v.15 no.19
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• pp.8251-8257
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• 2014

Apoptosis is one of main types of neural cell death and is reversible and is a major target of therapeutic interventions. However, detailed apoptotic cascades still need to be recognized. In present study, we determined the promotion of HIF-$1{\alpha}$ and survivin in brain samples of a mouse model of hypoxic-ischemia and in neuroblastoma SH-SY5Y cells post hypoxia treatment. Then gain-of-function and loss-of-function strategies were adopted to manipulate the HIF-$1{\alpha}$ in SH-SY5Y cells, and hypoxia-induced survivin upregulation and cell apoptosis were determined. Results demonstrated that the HIF-$1{\alpha}$ and survivin were significantly promoted in a mouse model of hypoxic-ischemia or in SH-SY5Y cells post hypoxia in vitro. Manually upregulated HIF-$1{\alpha}$ could promote the hypoxia-induced survivin upregulation and improve the hypoxia-induced SH-SY5Y cell apoptosis. On the other hand, the HIF-$1{\alpha}$ knockdown by RNAi reduced the hypoxia-induced survivin upregulation and cell apoptosis. Therefore, the present study confirmed the protective role of HIF-$1{\alpha}$ and survivin in the hypoxia-induced SH-SY5Y cell apoptosis, and the survivin upregulation by hypoxia is HIF-$1{\alpha}$-dependent. Promotion of HIF-$1{\alpha}$ and survivin might be a valuable stragegy for therapeutic intervention for hypoxic-ischemic encephalopathy.

• Journal of Korean Medicine for Obesity Research
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• v.14 no.2
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• pp.55-62
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• 2014

Objective: The prevalence of metabolic syndrome and type 2 diabetes is increasing worldwide. Mitochondrial dysfunction is known to be involved in insulin resistance and obesity, researches have been increasing highly. Astragali Radix extract (ARE) or its main components have been shown to perform comparably to insulin by significantly reducing blood glucose levels in animal models however, the influence on mitochondrial dysfunction are not well understood. Methods: ARE (0.2, 0.5 and 1.0 mg/ml) or metformin (2.5 mM) were treated in C2C12 after 6 day-differentiation. The expressions of adenosine monophosphate (AMP)-activated protein kinase (AMPK) and phosphorylation AMPK, peroxisome proliferators-activated receptror ${\gamma}$ coactivator $1{\alpha}$ ($PGC1{\alpha}$), nuclear respiratory factors 1 (NRF1), mitochondrial transcription factor (Tfam) and myosin heavy chain were detected with western blotting or polymerase chain reaction analysis. The morphological changes were also investigated. Results: ARE dose dependently increased phosphorylation of AMPK and respectively activated mRNA expressions of $PGC1{\alpha}$, NRF1 and Tfam which are mitochondrial biogenesis regulators. Furthermore, there were some morphologic differences of differentiated cells between ARE treatment and control. Conclusions: This study suggests that ARE has the potential to increase muscle mitochondrial function by activating AMPK and $PGC1{\alpha}$.

• Journal of the Korean Magnetic Resonance Society
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• v.17 no.1
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• pp.59-66
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• 2013

BldD, a developmental transcription factor from Streptomyces coelicolor, is a homodimeric, DNA-binding protein with 167 amino acids in each subunit. Each monomer consists of two structurally distinct domains, the N-terminal domain (BldD-NTD) responsible for DNA-binding and dimerization and the C-terminal domain (BldD-CTD). In contrast to the BldD-NTD, of which crystal structure has been solved, the BldD-CTD has been characterized neither in structure nor in function. Thus, in terms of structural genomics, structural study of the BldD-CTD has been conducted in solution, and in the present work, mainchain NMR assignments of the recombinant BldD-CTD (residues 80-167 of BldD) could be achieved by a series of heteronuclear multidimensional NMR experiments on a [$^{13}C/^{15}N$]-enriched protein sample. Finally, the secondary structure prediction by CSI and TALOS+ analysis using the assigned chemical shifts data identified a ${\beta}-{\alpha}-{\alpha}-{\beta}-{\alpha}-{\alpha}-{\alpha}$ topology of the domain. The results will provide the most fundamental data for more detailed approach to the atomic structure of the BldD-CTD, which would be essential for entire understanding of the molecular function of BldD.

• Korean Journal of Mathematics
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• v.24 no.1
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• pp.81-106
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• 2016

In this paper, we study the fractional iterates of the exponential function. This is an unresolved problem, not due to a lack of a known solution, but because there are an innite number of solutions, and there is no agreement as to which solution is "best." We will approach the problem by rst solving Abel's functional equation ${\alpha}(e^x)={\alpha}(x)+1$ by perturbing the exponential function so as to produce a real xed point, allowing a unique holomorphic solution. We then use this solution to nd a solution to the unperturbed problem. However, this solution will depend on the way we rst perturbed the exponential function. Thus, we then strive to remove the dependence of the perturbed function. Finally, we produce a solution that is in a sense more natural than other solutions.

• Publications of The Korean Astronomical Society
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• v.7 no.1
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• pp.25-30
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• 1992

Recent redshift surveys suggest that most galaxies may be distributed on the surfaces of bubbles surrounding large voids. To investigate the quantitative consistency of this qualitative picture of large-scale structure, we study analytically the clustering properties of galaxies in a universe filled with spherical shells. In this paper, we report the results of the calculations for the spatial and angular two-point correlation functions of galaxies. With ${\sim}20%$ of galaxies in clusters and a power law distribution of shell sizes, $n_{sh}(R){\sim}R^{-{\alpha}}$, ${\alpha}\;{\simeq}\;4$, the observed slope and amplitude of the spatial two-point correlation function ${\xi}_{gg}(r)$ can be reproduced. (It has been shown that the same model parameters reproduce the enhanced cluster two-point correlation function, ${\xi}_{cc}(r)$). The corresponding angular two-point correlation function $w({\theta})$ is calculated using the relativistic form of Limber's equation and the Schecter-type luminosity function. The calculated w(${\theta}$) agrees with the observed one quite well on small separations (${\theta}{\lesssim}2deg$).

• Bulletin of the Korean Mathematical Society
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• v.51 no.5
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• pp.1281-1289
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• 2014

In this paper, we consider the differential equation $$F^{\prime}-Q_1=Re^{\alpha}(F-Q_2)$$, where $Q_1$ and $Q_2$ are polynomials with $Q_1Q_2{\neq}0$, R is a rational function and ${\alpha}$ is an entire function. We consider solutions of the form $F=f^n$, where f is an entire function and $n{\geq}2$ is an integer, and we prove that if f is a transcendental entire function, then $\frac{Q_1}{Q_2}$ is a polynomial and $f^{\prime}=\frac{Q_1}{nQ_2}f$. This theorem improves some known results and answers an open question raised in [16].

• The Pure and Applied Mathematics
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• v.26 no.2
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• pp.59-73
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• 2019

In this paper, a version of the boundary Schwarz Lemma for the holomorphic function belonging to $\mathcal{N}$(${\alpha}$) is investigated. For the function $f(z)=z+c_2z^2+C_3z^3+{\cdots}$ which is defined in the unit disc where $f(z){\in}\mathcal{N}({\alpha})$, we estimate the modulus of the angular derivative of the function f(z) at the boundary point b with $f(b)={\frac{1}{b}}\int\limits_0^b$ f(t)dt. The sharpness of these inequalities is also proved.

• The Bulletin of The Korean Astronomical Society
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• v.38 no.1
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• pp.37.2-37.2
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• 2013

We present the galaxy luminosity function (LF) of the Abell 119 cluster. Deep images in u, g, r bands were taken using MOSAIC 2 CCD on a Blanco 4-m telescope at CTIO. Based on scaling relations at faint magnitudes and spectroscopy at bright magnitudes, accurate membership of galaxies is determined. The LF is fitted by a single Schechter function and a two components (Gauss + Schechter) function. Blue galaxies are well fitted by a single Schechter function with steep slope ${\alpha}$ ~ -1.55). Red galaxies in the inner, high density region are fitted by single Schechter function with shallow slope (${\alpha}$ ~ -1.30), while red galaxies in the outer, low density region are well fitted by a two components function. The different slope of LFs between the inner and outer seems to stem from the luminosity segregation of A119 indicating larger number ratio of luminous to faint ratio towards the cluster center. The different shape of LFs seems to be resulted from the different composition of luminous and faint galaxies among main-cluster, sub-cluster, and infall region.

• Proceedings of the Microbiological Society of Korea Conference
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• 2004.05a
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• pp.65-67
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• 2004

O-GlcNAcylation of p53 has been already identified and reported, but the function of O-GlcNAc on p53 has not been studied well. In this report, the general function of O-GlcNAc modification on p53 has been investigated using mouse fibroblast cell, L929. When streptozotocin (STZ), a non-competitive O-GlcNAcase inhibitor was treated to L929, O-GlcNAc modification level was dramatically increased on nucleocytoplasmic proteins, including p53. Because it has been already reported that $TNF\alpha$ induced the production of p53 in L929, $TNF\alpha$ was treated to obtain more p53. Approximately two times more amount of p53 was found from the cells treated STZ and $TNF\alpha$ simultaneously compared to the cell treated $TNF\alpha$ alone. The p53 increment in the presence of STZ was not caused by the induction of p53 gene expression. When new production of p53 induced by the $TNF\alpha$ was inhibited by the treatment of cycloheximide, O-GlcNAc modification decreased and phosphorylation increased on pre-existing p53 after $TNF\alpha$ treatment. But in the presence of STZ and $TNF\alpha$ at the same time, more O-GlcNAcylation occurred on p53, The level of ubiquitination on p53 was also reduced in the presence of STZ. Approximately three times less amount of Mdm2 bound to this hyperglycosylated p53. From this result it might be concluded that treatment of STZ to inhibit O-GlcNAcase increased O-GlcNAc modification level on p53 and the increment of O-GlcNAc modification stabilized p53 from ubiquitin proteolysis system.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.35 no.2
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• pp.156-164
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• 1990

A field experiment was conducted to study both the distribution characteristics of photosynthetically active radiation (PAR) in the soybean canopy and their relationships with dry matter production. The soybean cultivars 'Hwanggeumkong' and 'Paldalkong' were sown with the spaces of 60$\times$15cm and 30$\times$15cm at Suwon on May 20 and on June 20 in 1989. The ratio of PAR to the total shortwave radiation was estimated by the empirical equation derived from sunshine hours and direct incoming radiation. The functional relationships between the PAR interception and the leaf area index were expressed as a function of Beer's law. The extinction coefficients(k) in the functions ranged from 0.77 to 0.92. The values of k were greater at higher planting density, but they were affected neither by planting dates nor by varieties. The reflection ratio of PAR($\alpha$) was determined by the exponential function as below; $\alpha$=$\alpha$p-($\alpha$p-$\alpha$o) exp(-kㆍLAI) where $\alpha$p was the reflectance at the maximum LAI and $\alpha$o was that of the bare soil. The ap ranged from 0.025 to 0.035 and $\alpha$o ranged from 0.11 to 0.12, respectively. The reflected PAR ranged from 0.049 to 0.064 and the transmitted PAR ranged from 0.168 to 0.340 until maximum dry weights were observed. The slope from the linear regression of dry matter on absorbed PAR, conversion efficiency, ranged from 1.30 to 2.3g MJ$^{-1}$ during the growing season until maximum dry weight was reached. The total dry matter yield above ground (TDM) increased with the increases in the conversion efficiency. TDM was higher in Hwanggeumkong than Paldalkong and higher in the space of 30$\times$15cm than 60$\times$15cm, Paldalkong showed higher harvest index than Hwanggeumkong. than Hwanggeumkong.