• Title/Summary/Keyword: Allozyme

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A Sustematic Study of Korean Entomobryidae(Collembola, Insecta) Based on Cladistic Analysis of Phenotypic nd Allozyme Data (한국산 털보톡토기과(곤충강)의 표현형과 효소유전자형질에 대한 분지분석)

  • 이병훈;박경화
    • The Korean Journal of Zoology
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    • v.34 no.3
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    • pp.265-288
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    • 1991
  • 한국산 털보톡토기과(Collembola, Insecta)의 계통유연관계를 알아보기 위해 이 과에 속하는 3개속(Sinella, Entomobrya, Homidia)의 6종과 가시톡토기과의 1종(Tomocerus kinoshitai)에 대하여 모서식과 형태적 조사 외에 전기영동을 이용한 효소분석을 시리시하였다. 이들 모든 조사에서 32개의 형태형질과 33개의 모서식형질 및 80개의 효소유전자 형질을 얻어 분지분석하였는데, 이중 효소유전자에 대해서는 UPGMA방법도 적용하였다. 이러한 분석 결과 다양한 분지도를 얻었으나, Homidia종들은 언제나 두 개의 무리로 분리되었으며, Homidia munda의 두 아종이 가장 유연관계가 높았다. 반면에 Homidia koreana의 두 개체군은 다소 분리되어 같은 종으로 보기 의심스럽다는 소견을 얻었다. 또한 형태형질분석으로 얻은 계통수에서 가시톡토기과의 Tomocerus종이 털보톡토기과의 속들 사이에 끼어들어 나타나는데 이것은 형질에 따라 비중을 달리두는 평가방법이 아니기 때문에 나타난 것으로 추측된다. 본 연구 결과 대체로 외부형질과 모서식, 그리고 효소유전자의 세가지 유형의 형질들을 종합 분석할 때 도출되는 계통수가 자연분류에 가장 가까운 근연성을 보이는 것으로 판단되었다.

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Comparison of Mating Systems in Populations of Gleditsia japonica var. koraiensis

  • Huh, Man-Kyu
    • Journal of Ecology and Environment
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    • v.29 no.5
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    • pp.411-414
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    • 2006
  • The mating systems of two groups of natural populations of Gleditsia japonica var. koraiensis in Korea were determined using allozyme analysis. The result suggests that G. japonica var. koraiensis is predominantly outcrossing. The tm values of eight populations in Korea varied from 0.667 (Mdh-1) to 0.938 (ldh-1), giving an average 0.820. Population and individual outcrossing estimates were associated with flowering tree density or degree of spatial isolation. The reason for relatively low outcrossing rates of some populations could be attributed to reduction of effective population sizes of sib for the medicine, small population size, and isolation of flowering mature trees. The heterozygote excesses were observed in some natural populations, whereas other populations exhibited varying degrees of inbreeding and heterozygotes deficit. Thus, selection against homozygotes operated in the progeny populations throughout the life cycle.

Comparison of Reproductive Modes in Populations of Potentilla freyniana

  • Huh, Man-Kyu
    • Journal of Ecology and Environment
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    • v.30 no.3
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    • pp.225-229
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    • 2007
  • The mating systems of natural populations of Potentilla freyniana in Korea were determined using allozyme analysis. The result suggests that P. freyniana is outcrossing as well as employing vegetative reproduction by stolon (self-fertilization rate, s < 0.5). The values of the inbreeding coefficient of eight populations in Korea varied from 0.244 to 0.331, with an average value of 0.274. For eight natural populations, multi-locus estimates of outcrossing (tm) was 0.603 across 13 polymorphic loci, with individual population values ranging from 0.530 to 0.652. The relatively low outcrossing rates of some populations could be attributed to extensive vegetative reproduction by stolon and the isolation of flowering mature plants. Although P. freyniana usually propagated by asexually-produced ramets, I could not rule out the possibility that sexual reproduction occurred at a low rate because each ramet may produce terminal flowers. Although heterozygote excess was observed in some natural populations, most populations exhibited varying degrees of inbreeding and a heterozygote deficit.

Population Genetic Structure of Potentilla discolor Bunge, Rosaceae in Korea (한국내 솜양지꽃의 집단 유전 구조)

  • Huh, Man-Kyu
    • Journal of Life Science
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    • v.16 no.6
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    • pp.898-903
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    • 2006
  • The genetic diversity and population structure of fifteen Potentilla discolor Bunge populations in Korea were determined using genetic variations at 19 allozyme loci. Fourteen of the 19 loci (73.7%) showed detectable polymorphism. Genetic diversity at the species level and at the population level was high ($H_{ES}\;=\;0.215$, $H_{EP}\;=\;0.196$, respectively), whereas the extent of the population divergence was relatively low $(G_{ST}\;=\;0.069)$. Total genetic diversity values $(H_T)$ varied between 0.0 and 0.656, giving an average overall polymorphic loci of 0.292. The interlocus variation of genetic diversity within populations $(H_S)$ was high (0.274). On a per locus basis, the proportion of total genetic variation due to differences among populations $(G_{ST})$ ranged from 0.010 for Pgm-2 to 0.261 for Pgd-2 with a mean of 0.069, indicating that about 6.9% of the total allozyme variation was among populations. Wide geographic ranges, perennial herbaceous nature and the persistence of multiple generations are associated with the high level of genetic variation in P. discolor. The estimate of gene flow based on $G_{ST}$ was high among Korean populations of P. discolor (Nm = 3.36).

Ginseng Conservation Program in Russian Primorye:Genetic Structure of Wild and Cultivated Populations

  • Zhuravlev, Yu.N.;Koren, O.G.;Reunova, G.D.;Artyukova, E.V.;Kozyrenko, M.M.;Muzarok, T.I.;Kats, I.L.
    • Journal of Ginseng Research
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    • v.28 no.1
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    • pp.60-66
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    • 2004
  • “The Regional complex long-term program of restoration (reintroduction) of Primoryes ginseng population up to 2005” elaborated by Primorye governor administration, Regional Committee of Natural Resources and Russian Academy of Sciences operates in Russian Primorye. The Institute of Biology and Soil Science (IBSS) provides the scientific implementation of the program including the genetic analysis of extant ginseng populations, plant reproduction and off-spring identification. According to our investigations, the genetic resource of P. ginseng in Primorye is represented by three populations of wild-growing ginseng and a few pritate plantations. The results obtained by RAPD allowed concluding that the resource is dispersed among the wild and cultivated ginseng sub-populations in such a way that each of sub-populations studied has to be represented as a stock material to maintain species genetic variability. The allozyme analyses also showed that the small sub-populations of wild ginseng are characterized by unique genetic diversity and, therefore, they all need to be represented in reintroduction centers. Additionally the allozyme analysis discovered that the Blue Mountain and Khasan populations possess the most genetic diversity. So, at least one more reproductive ginseng unit has to be created besides two already existing reintroduction centers representing the Sikhote-Alin and the Blue Mountain populations.

Genetic Diversity and Population Structure of Potentilla freyniana in Korea (한국내 세잎양지꽃의 유전적 다양성과 집단구조)

  • Huh, Man-Kyu
    • Journal of Life Science
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    • v.17 no.7 s.87
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    • pp.877-881
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    • 2007
  • The genetic diversity and population structure of Potentilla freyniana in Korea were determined using genetic variations at 19 allozyme loci. Thirteen of the 19 loci (68.4%) showed detectable polymorphism. Genetic diversity at the population level was high ($H_{EP}$ = 0.270). Total genetic diversity values ($H_T$) varied between 0.190 and 0.584, giving an average overall polymorphic loci of 0.371. The interlocus variation of genetic diversity within populations ($H_S$) was high (0.354). On a per locus basis, the proportion of total genetic variation due to differences among populations ($G_{ST}$) ranged from 0.008 for Fe-2 to 0.310 for Gpi with a mean of 0.065, indicating that about 6.5% of the total allozyme variation was among populations. Wide geographic ranges, perennial herbaceous nature and the persistence of multiple generations are associated with the high level of genetic variation in P. freyniana. The estimate of gene flow based on $G_{ST}$, was high among Korean populations of P. freyniana (Nm =3.57). Although P. freyniana usually propagated by asexually-produced ramets, I could not rule out the possibility that sexual reproduction occurred at a low rate because each ramet may produce terminal flowers.

Lack of allozyme variation in the two carnivorous, terrestrial herbs Utricularia bifida and Utricularia caerulea (Lentibulariaceae) co-occurring on wetlands in South Korea: Inference of population history (한반도 남부 지방 습지에 같이 자생하는 식충 육상 초본 2종 땅귀개 및 이삭귀개 (통발과)의 알로자임 변이의 결여: 집단의 역사 추론)

  • Chung, Mi Yoon;Lopez-Pujol, Jordi;Chung, Myong Gi
    • Korean Journal of Plant Taxonomy
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    • v.47 no.4
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    • pp.297-303
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    • 2017
  • In central and southern Korea, the two small insectivorous, terrestrial herbs, Utricularia bifida and U. caerulea, often co-occur at wet locations (or in wetlands). The Korean Peninsula (with central China and northern Japan) constitutes the northern edge of their distribution, as their main range is subtropical and tropical Asia. The Korean populations of both species are very likely of post-glacial origin, given that warm-temperate vegetation was absent from the Korean Peninsula during the Last Glacial Maximum. Two hypotheses of the post-glacial colonization of the peninsula can be formulated; first, if current populations were founded by propagules coming from a single ancestral population (i.e., a single refugium), we would expect low levels of genetic diversity. Alternatively, if contemporary Korean populations originated from multiple sources (multiple refugia), we would expect high levels of genetic variation. To test which is more likely, we surveyed the degree of allozyme variation at 20 loci in ten populations for each of the two species from southern Korea. We found no allozyme variation within each species. However, their aquatic congener U. australis exhibited allozyme polymorphism across Japan (four polymorphic loci at three enzyme systems). We suggest that southern Korean populations of Utricularia bifida and U. caerulea were established by a single introduction event from a genetically depauperate ancestral population.