• Animal cells and systems
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• v.4 no.1
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• pp.77-85
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• 2000

This study has demonstrated the molecular variation of 5S rRNA genes in 15 Allium subgenus Rhizirideum and 1 Allium subg. Allium. For cloning of the 5S rRNA genes, PCR products were obtained from amplification with oligonucleotide primers which were derived from the conserved coding region of 5S rRNA genes. These amplified PCR products were cloned and identified by FISH and sequence analysis. The 5S rRNA loci were primarily located on chromosomes 5 and/or 7 in diploid species and various chromosomes in alloploid species. The size of the coding region of 5S rRNA genes was 120 bp in all the species and the sequences were highly conserved within Allium species. The sizes of nontranscribed spacer (NTS) region were varied from 194 bp (A. dektiude-fustykisum, 2n=16) to 483 bp (A. sativum). Two kinds of NTS regions were observed in A. victorialis var. platyphyllum a diploid, A. wakegi an amphihaploid, A. sacculiferum, A. grayi, A. deltoide-fistulosum and A. wenescens all allotetraploids, while most diploid species showed only one NTS region. The species containing two components of NTS region were grouped with different diploid species in a phylogenetic tree analysis using the sequences of 5S rRNA genes and adjacent non-coding regions.

• Journal of Plant Biotechnology
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• v.47 no.1
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• pp.15-25
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• 2020

Allium L. is one of the largest genera of the Amaryllidaceae family, with more than 920 species including many economically important species used as vegetables, spices, medicines, or ornamental plants. Currently, DNA barcoding tools are being successfully used for the molecular taxonomy of Allium. A total of 46 Allium species were collected from their native areas, and DNA was extracted using the IBRC DNA extraction kit. We used specific primers to PCR amplify matK. DNA sequences were edited and aligned for homology, and a phylogenetic tree was constructed using the neighbor-joining method. The results show thymine (38.5%) was the most frequent and guanine (13.9%) the least frequent nucleotide. The matK regions of the populations were quite highly conserved, and the amount of C and CT was calculated at 0.162 and 0.26, respectively. Analysis of the nucleotide substitution showed C-T (26.22%) and A-G (8.08%) to have the highest and lowest percent, respectively. The natural selection process dN/dS was 1.16, and the naturality test results were -1.5 for Tajima's D and -1.19 for Fu's Fs. The NJ dendrogram generated three distinct clades: the first contained Allium austroiranicum and A. ampeloprasum; the second contained A. iranshahrii, A. bisotunense, and A. cf assadi; and the third contained A. rubellum and other species. In this study, we tested the utility of the matK region as a DNA barcode for discriminating Allium. species.

• Korean Journal of Plant Taxonomy
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• v.51 no.2
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• pp.141-146
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• 2021

Allium stenodon (Amaryllidaceae) is reported as a returning member of the Korean flora. This species proved to be the only member of the section Sikkimensia among Korean Allium species, and it is easily distinguished by its cylindrical bulbs with fibrous to sometimes subreticulate tunics and blue to purple-blue perianth. Previously recognized A. baekdusanense is treated as an additional synonym of A. stenodon. Photographs and complete descriptions of A. stenodon are provided together with a dichotomous key to the Korean Allium species. In addition A. stenodon is newly recorded in Jilin province in China.

• Korean Journal of Plant Taxonomy
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• v.33 no.3
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• pp.295-301
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• 2003

We reported an unrecorded species of the genus Allium (Alliaceae) which is growing along riversides in the central part of Korean peninsula. A. longistylum Baker, has been known to distribute only in China. The new common name, 'Gang-bu-chu', was given considering the habitat characteristics such as growing riversides in Korea. In this study, we described morphological characters, and provided illustrations as well as photographs of the habitat.

• Journal of Forest and Environmental Science
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• v.27 no.1
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• pp.33-37
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• 2011

This study was carried out to establish a proper cultivation site and diagnose the drought-tolerance of Allium ochotense leaves and Allium microdictyon leaves by using pressure - volume curve method. The Allium microdictyon leaves had more lower osmotic water potential ${\psi}osat$, and osmotic water potential at turgor loss point ${\psi}otlp$ than Allium ochotense leaves. Then, the Allium microdictyon leaves was slightly higher drought-tolerance than Allium ochotense leaves. Therefore, We suggest that growth of the two Allium species was appropriate for relative moisture forest.

• Journal of Plant Biology
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• v.20 no.2
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• pp.71-76
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• 1977

The present paper was carried out to clear up the polyploidal constitution and the banding pattern of three species in tetraploidal Allium(X=8) through the studies of meiosis, karyotype and G-bands. From the meiotic data and G-banding patterns obtained under this study, it is confirmed that A. tuberosum and A. chinense are autotetraploids, and A. senescens is allotetraploid. Some aneuploids out of the employed species were found; A. senescens is 2n=34, and A. chinense is 2n=33. The chromosome types of these species are meta-and submetacentrics except the sat-chromosomes and the f-chromosomes. G-bands of these species are generally located in the end of each arm. A. senescens is similar in the quantity of heterochromatin with A. chinense, but A. tuberosum has a little than the other species. The quantity of heterochromatin is higher in small groups of chromosome than large ones, and higher in short arms than long arms.

• Journal of the East Asian Society of Dietary Life
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• v.11 no.1
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• pp.33-43
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• 2001

The effects of the addition of herbs belonging to Allium species on the quality and storage characteristics of kamaboko were studied. The herbs employed in the study were garlic (Allium sativum for.Pekinenese Makino), leek(.Allium tuberosum Roth), onion(Allium cepa Linnaeus), and onion skin. water activity(Aw) , pH, TBA, VBN, microbial load, textural characteristics and sensory evaluation were tested. 1. Aw of all the samples decreased on storage, the Aw on lower day ranged from 0.937~o.950. All thesamples containing herbs retained the pH 6 ~7 during the entire storage period even though it decreased gradually on storage. 2. In general, TBA and VBN of the samples containing herbs were lower than the control. The sample containing 3% onion skin showed the lowest TBA and VBN value. 3. The total plate count of the samples containing herbs was low compared to the control even though the total count increased during the storage. The samples containing 3% garlic and 3% onion skin showed the lowest total plate count on 20 days of storage. 4. The sample with garlic showed remarkably low value in sensory evaluation. The samples containing onion and onion skin, however, reached to the high sensory points as storage period increased. They received high points in taste as well as overall acceptance.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.46 no.4
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• pp.328-333
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• 2001

Allium is one of the largest genera, which has more than 700 species. PCR by URP (universal rice primer) primers was carried out to get phylogenetic information on 26 species, 62 accessions of subgenus Rhizirideum. The accessions were divided into seven groups at 0.76 similarity level. A. tuberosum (Chinese chives) and A. ramosum represented high similarity of 0.91. A. montanum, A. nutans, A. senescens, A. libani, A. odorum, A. austrosibiricum, and A. narcissiflorium grouped at 0.80 similarity. Some of the wild species, such as A. prostratum, A. polyrhizum, A. odorum, and A. mongolicum, showed different band patterns according to polyploidy, occurrence of B-chromosome, collection site, and origin.

• Korean Journal of Agricultural and Forest Meteorology
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• v.11 no.4
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• pp.252-255
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• 2009

In this study we investigated the natural habitats of Allium grayi, Allium monanthum, Allium tuberosum' and Allium schoenoprasmum to develop a fundamental database for their breeding and cultivation. Both Allium grayi and Allium monanthum were growing wild mainly in the areas with the altitude of ${\leq}\;300\;m$. The natural habitats of Allium grayi and Allium monanthum were limited to the altitude of 1,000 and 800 m, respectively. Allium thunbergii was growing at a wide range of altitude from the lowlands (${\geq}\;100\;m$) to the high elevated areas (${\leq}\;1,000\;m$) whereas Allium maximowiczii was growing wild only at the high altitude of ${\geq}\;900\;m$. The number and the size of the natural habitat of Allium grayi were greater than those of Allium monanthum. The natural habitats of Allium grayi were in the Gyeonggi, Gangwon, Jeolla, and Gyeongsang Provinces whereas those of Allium monanthum were found mainly in the Chungcheong and Jeju Provinces. Allium grayi was growing wild in both inland and seaside districts whereas Allium monanthum was mainly in the inland areas.

• The Korean Journal of Mycology
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• v.23 no.2 s.73
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• pp.139-143
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• 1995

A species of Cladosporium isolated from Allium victorialis var. platyphyllum Makino differs from C. allii and C. allii-cepae by catenate, multiseptate conidia and aseptate conidiophores. Comparable Cladosporium spp. on other substrates are unknown. Therefore, this fungus must be considered as a new, undescribed species, viz. Cladosporium alliicola sp. nov.