• Journal of Periodontal and Implant Science
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• v.25 no.3
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• pp.539-556
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• 1995

Periodontal therapeutic modalities should be re-establishing and regenerating the periodontal tissue previously lost to the disease. To achieve periodontal regeneration, periodontal ligament cells must selective migrate to the deneded root surface, attached and proliferated it. Local pH concentration is one of the most factors that periodontal regeneration. The aims of this study were to examine on biological effects of pH to the human periodontal ligament cells in vitro, especially on the cell morphology, attachment, activity, vitality and viability. Human periodontal ligament cells were cultured from extracted tooth for non-periodontal reason. Immediately after extraction, any soft tissue adhering to the cervical parts of the roots was carefully removed with a sterile curette. To produce different pH levels in the media, Eagle's MEM was adjusted from pH 6.6 to 8.2 in 0.2 intervals with 1 M NaOH and 1 N HCl. After cultivation, Then, Periodontal ligament cells were cultured at pH ranging from 6.6-8.2. attachment assay was done at 1, 2 day incubation and activity assay was done at 1, 2, 3 day incubation. The experiments were evaluated by scaning electron microscopic techniques (HITACHIX-650 Scaning Electron Microanalyzer, Tokyo, Japan), MTT assay, and the cultured periodontal ligament cells were fixed in neutral formalin for 24 hours and immunohistochemically processed by PCNA for proliferating ability. The surviving cells in the medium showed slightly increased volume and widening intercellular distances at low concentration of pH than control group (pH 7.4), and apparently shrinkage at high concentration of pH than control group (pH 7.4). The results of the statistical analysis from the experiment on attachment, vitality and viability were as follows. Attachment of periodontal ligament cells at 1st and 2nd day, similar attachment rate of low concentration pH compared with control value (pH 7.4). But above pH 8.0, attachment rate were statistically significant decrease from control value(P<0.05). Periodontal ligament cell's activities were maximum at pH 7.6 by MTT assay. Similar with control value at low concentration of pH. But, the activities were statistically significant decrease at high concentraration of pH(P<0.05). Cellular proliferating rate (PCNA index) were statistically significant decrease from control value at low and high concentration of pH(p<0.05). This results suggested that hjgh concentration pH, in other words, alkali pH was cytotoxic effects on human periodontal ligament cells in vitro.

• Journal of the Korean Society of Food Science and Nutrition
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• v.38 no.9
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• pp.1139-1144
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• 2009

The purpose of this study is to determine the possibility of using Pueraria flos as natural health food source. To accomplish this purpose, the contents of general and antioxidative nutrients of Pueraria flos a were measured. The contents of carbohydrate, crude protein, crude lipid and ash were 69.2%, 19.9%, 2.2% and 8.9%, respectively, and calories was 340.4 kcal. Total dietary fiber was 85.1% of total carbohydrates. The percentages of water soluble dietary fiber to insoluble dietary fiber were 12.0% and 46.7%, respectively. The protein contained 18 different kinds of amino acids. The contents of essential and non-essential amino acids were 5.0 g and 6.7 g. The Ca was the largest mineral followed by K, Mg, and P, which means Pueraria flos is alkali material. The contents of saturated fatty acids, monounsaturated fatty acids and polyunsaturated fatty acids were 25.37%, 33.61% and 35.68%, respectively. Therefore, the amount of the total unsaturated fatty acid was higher than that of any other plant. The antioxidant activity of 70% ethanol extract and fractions of the Pueraria flos has been determined by the scavenging of the stable radical DPPH; the result showed that the ethyl acetate fraction was the most active, as the amount required for 50% reduction of DPPH after 30 mins ($RC_{50}$) was 109.9 ${\mu}g$, followed by 70% ethanol extract (217.3 ${\mu}g$), hexane fraction (134.5 ${\mu}g$), chloroform (116.7 ${\mu}g$), butanol faction (129.8 ${\mu}g$) and aqueous fraction (473.5 ${\mu}g$).

• Korean Journal of Fisheries and Aquatic Sciences
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• v.5 no.1
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• pp.29-38
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• 1972

The protein composition of abalone muscle was estimated with the following result: on a series of samples analyzed, water-soluble protein, $19\~22\%$, salt-soluble protein, $27\~39\%$: alkali-soluble protein, $20\~26\%$ : and stroma $20\~28\%$ : respectively. It was demonstrated by ultracentrifugal analysis that approximately $65\%$ of the salt-soluble protein is accounted for by paramyosin, $30\%$ by actomyosin, and $5\%$ by myosin, respectively. The ultracentrifugally homogenous paramyosin was prepared by BAILEY's ethanol-dried method. It showed a $S^{\circ}\;_{20,\;{\omega}$ of 3.14s, and was completely salted in with KCl beyond $0.35{\mu}$. The intrinsic viscosity at $25^{\circ}C$ was estimated at 3.1. The paramyosin is rich in several amino acids such as arginine, aspartic acid, glutamic acid, etc., and lacking of both proline and tryptophane, in rough accord with other paramyosins reported. The abalone paramyosin did not show ATPase activity over a pH range of 5 to 9,5 even in the presence of Ca++ or Mg++. So was the case with the paramyosin specimen prepared by BAILEY's wet-extraction method.

• Korean Journal of Food Science and Technology
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• v.25 no.1
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• pp.22-27
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• 1993

Crude ${\beta}-glucan$ extracted from Barley was purified by stepwide enzyme treatment with thermostable ${\alpha}-amylase$, amyloglucosidase and protease. The thermal properties of Barley ${\beta}-glucan$ were investigated by Differential Scanning Calorimetry. Three endotherms have been observed on DSC thermograms of Barley ${\beta}-glucan$. The first endotherm which produced the gelatinization phenomena commonly observed in Barley ${\beta}-glucan$ became the focus of this study. The temperature range and the enthalpy of gelation exhibited maximum values with increasing concentration of Barley ${\beta}-glucan$. Gelating Barley ${\beta}-glucan$ registered an enthalpy of approximately 0.23 cal/g and exhibited onset temperature (To), peak temperature (Tp) and conclusion temperature (Tc) of $48.8^{\circ}C,\;61.2^{\circ}C\;and\;78.5^{\circ}C$ respectively. The temperature and enthalpy of gelatinizing Barley ${\beta}-glucan$ at both alkali and acid conditions were lower than those at pH 7. With salt present, the Tp and Tc of gelating Barley ${\beta}-glucan$ produced lower temperatures than in conditions where salt was absent, and the enthalpy abruptly decreased. However, increasing salt concentrations did not affect the gelation temperature and the enthalpy of Barley ${\beta}-glucan$. The 'true melting' temperature of Barley ${\beta}-glucan$ was near $184^{\circ}C$ and the melting enthalpy was approximately 34.6 cal/g. The Barley ${\beta}-glucan$ decomposition temperature was in the range of $316^{\circ}C{\sim}346^{\circ}C$.

• Journal of the Korean Chemical Society
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• v.48 no.2
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• pp.129-136
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• 2004

Titanium and [titanium+Na(K)] substituted 11${\AA}$ tobermorites solids synthesized under hydrothermal conditions at 180$^{\circ}C$ exhibit cation exchange properties toward heavy transition metal cations, such as Fe$^{2+},\;Zn^{2+},\;Cd^{2+}\;and/or\;Pb^{2+}$. The amount of heavy metal cations taken up by these solids was found in the order: Fe$^{2+}>Zn^{2+}>Cd^{2+}>Pb^{2+}$, and reached maximum at 10% [Ti+K]-substituted tobermorite. The total cation exchange capacity of the 10% Ti+Na (K) - substituted tobermorites synthesized here range from 71 to 89 meq/100 g, and 50-56 meq/100g for Ti-substituted only. Results indicated that 10% [Ti+K] substitution exhibit cation exchange capacity more 2.4 times than the unsubstituted-tobermorite. This is due to the increase of the number of active sites on the exchangers. The incorporation of Ti and/or [Ti+Na(K)] in the lattice structure of synthesized tobermorites is due to exchange of Ti$^{4+}{\Leftrightarrow}2Ca^{2+}\;and/or\;Ti^{4+}+2Na^+(K^+){\Leftrightarrow}3Ca^{2+}$, respectively. The mechanism of Ti and [Ti+Na(K)] incorporations in the crystal lattice of the solids during synthesis and the heavy metal cations uptaken by these solids is studied.

• The Journal of the Petrological Society of Korea
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• v.15 no.2 s.44
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• pp.90-105
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• 2006

The Cretaceous volcanic rocks in the study area represented by andesitic rocks occupy eastern part of the Kyeongsan Caldera. The volcanic rocks comprise andesite I, andesitic tuff, andesite II, and andesitic tuff breccia in their stratigraphic succession, and andesitic porphyry. Andesite I is distinguished from andesite II in their color, texture, phenocryst mineralogy and petrochemisty. In outcrops, andesite I is compact and dark-green, and andesite II is brick red in color and porphyritic in texture. In their phenocryst mineralogy, andesite I contains olivine phenocryst in addition to plagioclase and pyroxene which occur in both of andesites. Compared to andesite II, andesite I is higher in $SiO_2$ and $K_2O$ contents and lower in CaO, MgO, MnO, $TiO_2,\;Fe_2O_3$, and $P_2O_5$. Major elements petrochemistry shows that magma series of the volcanic rocks spread widely from calc-alkaline to alkaline series. On the other hand, immobile trace elements petrochemistry shows that the magma series is calc-alkaline without exception, suggesting that the volcanics has experienced more or less alkali enrichment after their eruption. Trace element diagrams for discrimination of tectonic setting show that the volcanics of the study area might be originated from calc-alkaline continental volcanic arc.

• The Journal of the Petrological Society of Korea
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• v.4 no.2
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• pp.91-103
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• 1995

The study area is located at the middle part of Daebo granitic batholith in the Gyeonggi massif. The geology of the area is mostly composed of Precambrian gneiss complex, coarse- grained middle Jurassic and fine-grained early Cretaceous biotite granites, and Cretaceous small stocks and dykes. The gneiss complex consists mainly of banded gneiss, granitc gneiss, some schist and quartzite. The coarse-grained granite can be divided into greyish granite(Gg1 in the margin and slightly pinkish granite(Gp) in the center. The former is hornblende biotite granite characterized by basic clot and xenolith. The latter is generally garnet biotite granite containing only poor basic clot. The fine-grained granite intruded the coarse-grained granite. The K/Ar biotite ages from the granites belong to middle Jurassic and early Cretaceous. The K/Ar biotite ages and geochemical compositions indicate that Gg and Gp were differenciated from a single magmatic body. The granites are calc-alkali and metaluminous-peraluminous. They are S-type(i1menite series) and partly I-type granitedmagnetite series) formed by melting of relatively fixed source composition. Their tectonic settings belong to the compressional suits and VAG of continental margin.

• The Journal of the Petrological Society of Korea
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• v.27 no.2
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• pp.97-104
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• 2018

The U-Pb isotopic and rare earth element compositions of zircon were measured using a SHRIMP from a tonalitic gneiss sample DE43 in Daeijak Island, central Korea. Zircon crystals, up to ${\sim}300{\mu}m$ in diameter, rarely contain thin overgrowth rims. In contrast to Paleoproterozoic cores, the $^{206}Pb/^{238}U$ ages of $256{\pm}23Ma(1{\sigma})$, and $221{\pm}7Ma(1{\sigma})$ were yielded from two spot analyses on the overgrowth rims of zircon. The rims are geochemically characterized by low Th/U ratios (<0.01) and strongly depleted light rare earth elements. The Permian-Triassic apparent ages of zircon are consistent with the $^{208}Pb/^{232}Th$ ages dated from allanite ($227{\pm}7Ma(t{\sigma})$) in the same sample within uncertainties, indicating an equilibrium growth of allanite and zircon at ~227 Ma. On the other hand, the younger $^{208}Pb/^{232}Th$ and $^{206}Pb/^{238}U$ ages ($213{\pm}4Ma(t{\sigma})$ and $186{\pm}9Ma(t{\sigma})$, respectively) of allanite may result from Pb loss due to the infiltration of alkali fluids from Late Triassic and Jurassic granitoids nearby.

• The Journal of the Petrological Society of Korea
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• v.27 no.2
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• pp.73-84
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• 2018

In the northern Cheongsong, there are occurred igneous intrusions: Cheongsong dike swarm, Jungtaesan laccolith, Galpyeongji stock. The swarm is composed of rhyolitic dikes that have developed many various spherulites. The dikes represent an geometrically radical pattern centering the Galpyeongji stock, but also geochemistry of the intrusions indicate the swarm source. Here we report the compositional data for 28 samples from the three intrusions. All of the intrusions belong to rhyolitic composition, but according to compositional correlation, there are considerable overlaps between intrusion compositions. In particular, the Cheongsong dike swarm is divided into several dike groups by rock color and shows compositional diversity, but the composition of the dikes generally overlap with compositions of other intrusions. The Jungtaesan laccolith is enriched in alkali, $K_2O$ and $Al_2O_3$ and depleted in $Fe_2O_3{^t}$, $TiO_2$ and REE compared to the Cheongsong dike swarm. In contrast, the Galpyeongji stock is narrow in composition range, and commonly has sharp compositional overlaps with the Cheongsong dike swarm. According to the compositional correlations, the stock is considered to be a source of the swarm and it is connected to an episode of volcanism.

• Journal of Plant Biology
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• v.13 no.1
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• pp.13-24
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• 1970

This investigation was one of the series of ecological studies on the plant communities of Quelpart Island which was held in 1969. It was aimed at studying the ground vegetation of Torreya nucifera and the soil properties of the ground in which it grows. Observaton of the flora in a place where wild growth of Crinum maritimum, soil properties and distribution of micro-organisms in a place where wild growth occurs, were also made. 1) A few kinds which have important values among the ground vegetation fo Torreya nucifera forest are Liniope graminifolia, Convallaria keiskei, Sasaquelpaertensis, Carex kingiana, Pteridium aquilinum, Pyrola japonica, Hedera tobleri, etc. 2) While the studies of 25 Quadrats were being held, 29 species in A plot, 30 species in B plot, and 26 species in C plot have been found. 3) The kind which has the highest important value among the species located in naked plot(outside of Torreya nucifera forest) is Zoysia japonica and 18 other kinds were found. 4) The soil fertility through all depths are found higher in the forest soil than in that of open places. 5) P2O5, K, and total exchangeable bases etc. have shown the difference of contents according to the depth and generally had lower value as much as it goes down. 6) The content of N was a lot higher than any other forest soil and P2O5, K and total exchangeable bases seemed to be a little lower. 7) The overall flora of Todo consists of 71 species (9 species of wood plant and 62 species of herbs) added 28 kinds of non-reported species. 8) Themineral contents are the highest at a place of wind growth of Crinum maritimum which has much organic matter. The subsoil of this place is more fertile than surface soil. 9) The soil of Todo which is sandy soil, consists of shellfish shell and quartz, and has low fertility and alkali. 10) In the total occurrences of micro-organisms, bacteria was the greatest in number followed by actinomycete and fungi, in that order. 11) A survey of the soil depth and the ecological distributional pattern of micro-organismsm revealed that the number of micro-organisms is the greatest on the surface, gradually decreasing in proportion to the depth. 12) It was found that a comparatively greater number of micro-organisms occurred when the soil showed an adequate increase in water content.