• Journal of Nutrition and Health
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• 제37권1호
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• pp.15-22
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• 2004

The purpose of this study was to investigate the effect of butanol (BuOH) fraction of Alisma canaliculatum (Ac) and/or selenium (Se) treatment on glycogen level, lipid metabolism and lipid peroxidation in streptozotocin (STZ)-induced diabetic rats. Male Sprague-Dawley rats were assigned to one of the five groups: normal, STZ-control, and three experimental groups (Ac group, Ac-Se group, and Se group). Diabetes was experimentally induced by intravenous administration of 45 mg/kg of STZ in citrate buffer. The BuOH fraction of Ac (400 mg/kg bw) was orally administered for 3 weeks. The Se group were fed a AIN-93 recommended diet mixed with Na$_2$SeO$_3$ (2 mg/kg diet). The liver glycogen level of Ac and Ac-Se groups were significantly higher, when compared with the STZ-control groups. The muscle glycogen level was not significantly differ among all groups. The levels of liver triglyceride were higher in Ac-Se group than the STZ-control group. Pancreas protein levels were significantly increased in Ac-Se group than STZ-control group. The concentration of liver malondialdehyde (MDA) was significantly decreased in Ac and Se groups and decreased in Ac-Se group. Administration of BuOH fraction of Alisma canaliculatum and selenium supplementation increased the liver glycogen and triglyceride levels, and reduced peroxidative liver damage in STZ induced diabetic rats. These results suggest that treatment with a BuOH fraction of Alisma canaliculatum in combination with selenium has no synergistic antioxidative effect. Selenium supplementation may lead a decrease MDA of liver in diabetic rats.

• 한국조경학회지
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• 제32권3호
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• pp.43-50
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• 2004

This study looked into the water quality improvement effect of reed (Phragmites communis), bur reed (Sparaganium stoloniferum), Alisma canaliculatum, and Hydrocharis dubia in order to develop native hydrophytes suitable to natural-type wetlands for ecological restoration. The result confirms that the concentration of wastewater was lowered in most plants indicating a possibility in wastewater treatment. Among four species, reed (Phragmites communis) and bur reed (Sparaganium stoloniferum) were most effective in removing a majority of contaminants. Considering that reed (Phragmites communis) is a species that has been used most often among hydrophytes, bur reed (Sparaganium stoloniferum), which is a native species in Korea, showed equivalent level of outstanding effect. In the physiological and growing activity and its relations to water quality improvement effect, each species appeared differently. Reed (Phragmites communis) and bur reed (Sparaganium stoloniferum) were best also in physiological and growing activity. In the case of Hydrocharis dubia, growth was strong in polluted water environment but its physiological activity was poor, indicating that it was under stress. The growth and physiological activity of Alisma canaliculatum were also poor, indicating that it too is being stressed. However, measuring organic contents in the plants of each species shows that the organic contents of Alisma canaliculatum was high. Alisma canaliculatum is a species effective in removing pollutants in water, although it is under stress. A unique phenomenon in removing water pollution with plants is that each species removed different pollutants. In the case of Alisma canaliculatum, which was relatively poor in removing pollution, it was remarkable in reducing nitrogen and phosphorus. Hydrocharis dubia was also effective in eliminating floating matter.

• 대한한방내과학회지
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• 제39권6호
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• pp.1181-1190
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• 2018

Objective: This study investigated the effect of purified medical herb extracts such as Alisma canaliculatum and Polyporus umbellatuson adipogenic differentiation of human bone marrow derived mesenchymal stromal stem cells (hBMSCs). Methods: Two different medical herb were extracted using hot distilled water. The optimal concentration of extracts were fixed at 100 ng/ml by means of cell viability and cytotoxic assay. To test the adipogenic differentiation ability of extracts, we induced the adipogenesis of hBMSCs for 21 days. At day 5, the cell was harvested to check mRNA and protein expression level of adipogenic related factors. The efficacy of lipid droplet formation was evaluated using the oil-red O staining method at days 21. Results: Two different medical herb extracts have no toxicity on hBMSCs. And two different medical herb extracts significantly decreased formation of lipid droplet compared with control groups in hBMSCs. The A. canaliculatum extract group showed the lowest mRNA and protein expression level of adipossgenic related transcription factors. This data suggests that extract of A. canaliculatum and P. umbellata decrease the adipogenic differentiation of hBMSCs. Conclusions: Our findings indicate that water-extract of A. canaliculatum and P. umbellata will be useful therapeutic reagents for prevention of obesity related disease such as diabetes, hyperlipidemia, coronary artery disease, and osteoporosis.

• Journal of Nutrition and Health
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• 제34권6호
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• pp.619-625
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• 2001

Diabetes mellitus was induced in male Sprague-Dawley rats weighing 200-245g by injection of streptozotocin(STZ) into the tail vein at a dose of 45mg/kg and were divided into seven groups ; normal, diabetic control, and five experimental groups(fractions of hexane, chloroform(CHCl$_3$), ethylacetate(EtOAc), butanol(BuOH) and water($H_2O$)). The rats of all groups were fed on AIN-93 diet and the five experimental groups were orally administered each fraction for 14 days. The body weight and diet intake were monitored daily. The plasma levels of glucose, insulin, cholesterol, triglyceride, HDL-cholesterol, free fatty acid and aspartate and alanine aminotransferase activities were analyzed. Diabetic rats showed the lower weight gain compared to the normal rats. The plasma glucose levels of the CHCl$_3$, and $H_2O$ fraction groups were significantly lower than the other experimental groups. The plasma insulin level of the CHCl$_3$ fraction group was much higher than that of diabetic control group. The plasma cholesterol levels were increased in all the experimental groups. The groups of hexane, BuOH and H2() fractions showed the lower plasma triglyceride levels compared to diabetic control group. The plasma free fatty acid levels were not significantly different in all groups. HDL-cholesterol levels were definitely higher in hexane, CHCl$_3$ and EtOAc fraction groups than that of diabetic control group. In conclusion, administration of CHCl$_3$ and $H_2O$ fractions of methanol extract of Alisma canaliculatum exhibited hypoglycemic effects in STZ induced diabetic rats, showing the possibility of therapeutic use of Alisma canaliculatum to the diabetes mellitus.

• Nutritional Sciences
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• 제6권2호
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• pp.85-93
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• 2003

The purpose of this study was to investigate the effects of a butanol fraction of fraction of Alisma canaliculatum All. Braun et Bouche (Ac), and of selenium (Se), on plasma gllucose and lipid levee in streptozotocin (STD-induced diabetic rats. Male Sprague-Dawley rats, fed the AIN-93 recommended diet, were divided into five groups: a non-diabetic control group (no STZ treatment), and four 572-induced diabetic groups which consisted of a diabetic-control group, an Ac-treated group, an Ac-Se treated group, and a Se-treated group. Diabetes was induced in the rats by an injection of STZ into the tail vein at a dose of 45 mg/kg body weight. The butanol (BuOH) fraction of Ac was orally administered at a rate of 400 mg/kg body weight for 21 days to both the Ac and Ac-Se groups. The supplementation of selenium in the Se and Ac-Se groups was achieved by adding (freshly, every day) 2 mg of Se as Na$_2$SeO$_3$ per kg of feed. The rats'body weights and hematocrit (Hct) levels were measured, along with plasma levels of glucose, insulin, cholesterol, HDL-cholesterol, triglyceride (TG), and free fatty acids (FFA). Aminotransferase activities were also analyzed. The non-diabetic rats gained weight, while the diabetic rats lost weight - except in the Ac-Se group, which maintained their initial weight. The blood glucose levels of the Ac group and the Se group were significantly lower than for the diabetic-control group. The plasma triglyceride levels were lowered when both Ac and Se were administered to diabetic rats. The concentrations of plasma FFA in the Ac-Se group were significantly lower compared with the diabetic-control group. Plasma cholesterol levels and alanine aminotransferase activity in the Ac, Ac-Se, and Se groups were significantly lower when compared with the diabetic-control group. Aspartate aminotransferase activity was significantly lower in the Se group compared to the other diabetic groups. These data show that treatment with a butanol fraction of Ac in combination with Se has no synergistic effect. Plasma glucose levels tended to be low when Se was administered to diabetic rats. Supplementation of Se in diabetic rats did not elicit a significant increase in plasma insulin levels or result in hypolipemic effects.

• 한국식품조리과학회지
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• 제18권3호
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• pp.300-308
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• 2002

The purpose of this study was to investigate the effect of chloroform(CHC1$_3$) fraction of Alisma canaliculatum All. Braun et Bouche(Ac) with selenium(Se) on the plasma glucose and lipid levels in streptozotocin(STZ)-induced diabetic rats. Male Sprague-Dawley rats were divided into five groups. The normal and diabetic rats were separated into four groups: the STZ-control group, the Ac group, the Ac-Se group and the Se group. Diabetes was induced in the male rats by an injection of STZ into the tail vein at a dose of 45 mg/kg. The CHCl$_3$fraction of Ac(250 mg/kg) was administered orally for 14 days. The supplementation was achieved with the AIN-93 recommended diet by adding 2 mg/kg diet of selenium as Na$_2$SeO$_3$. which was prepared freshly everyday. The body weight, hematocrit(Hct), glucose, insulin, cholesterol, HDL-cholesterol, triglyceride(TG) and free fatty acids(FFA) concentrations in plasma were measured. The aminotransferase activities were also analyzed. The changes of body weight in the experimental groups were not significantly different from that of the STZ-control group, but diabetes hyperphagia accompanied changes with body weight loss in Ac-Se group. The levels of Plasma cholesterol were not significantly different among the experimental groups. The concentrations of FFA in the Ac-Se group increased significantly compared with the STZ-control group. The effect of Se alone significantly increased aspartate aminotransferase activity and alanine aminotransferase activity. The results showed that the treatment of CHCl$_3$fraction of Ac in combination with Se has no synergistic effect. There was a tendency for the plasma glucose levels to decrease when Se was administered into diabetic rats. Supplementation of Se in diabetic rats did not elicit a significant increase in plasma insulin levels and exhibited hypotriglyceridemic effect.

• Applied Biological Chemistry
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• 제13권1호
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• pp.81-85
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• 1970

한국답토양십종(韓國畓土壤十種)을 시료(試料)로하여 유효인산정량(有動燐酸定量)에 적합한 화학적(化學的) 추출방법(油出方法)을 찾고저 A-value인(燐)-32 추적자법(追跡子淪)에 의한 유효인산정량치(有效燐酸定量値)를 표준으로 하여 시험한 결과 다음과 같은 결과(讀果)를 얻었다. 1 A·value는 Fe-P(인산철(燐酸鐵))와만 1%수준(水準)의 유의상관(有意相鬪)이 있으므로 답토양(畓土壤)의 유효인산(有刻燐酸)은 Fe·p계(系)에 의하여 지배(支配)되고 있다고 볼 수 있다. 2. 본(本) 실험(實驗)에 사용(使用)한 6개침출법(個浸出法)은 Fe-P와 상안(相關)이 없으므로 답토양유효인산정량법(容土壤有效燐酸定量法)으로 적합하지 않다. 3. 답토양유효인산(容土壤有效燐酸)에 적합한 침출법(浸出法)은 Fe-P계(系)의 환원강도(還元强度)를 측정(測定)할 수 있는 것이어야 하며 침출강도(浸出强度)는 평균(平均) 270 ppm 으로 추정(推定)된다. 4. Bray No. 2-p는 Lancaster-p, Spurway-p, Truog-p 및 (Ca+Al)-p와 5% 수준(水準) 이상(以上)에서 유의상관(有意相關)이 있고 Al-P는 Olsen-P 및 Lancaster-p와, Ca-p는 Spurway-p와 1% 수준(水準)에서 유의상관(有意相關)이 있다.

• 한국동물위생학회지
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• 제33권4호
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• pp.375-385
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• 2010

An investigation was conducted to evaluate the effects of supplementing Alisma canaliculatum, Viscum album and Cornus officinalis probiotics on the growth performance and immune response in growing pigs. This experiment was conducted using 120 pigs (crossing of Landrace${\times}$Yorkshire and castrated) which were assigned to 5 treatments in 3 replications with 8 pigs per replications. The dietary treatments were NC group (without antibiotics), PC group (basal+Oxytetracycline 50ppm), AC group (basal+A. canaliculatum 0.5%), VA group (basal+V. album 0.5%) and COP group (basal+C. officinalis probiotics 0.5%). The initial body weights of pigs were 35kg on average and the experiment lasted for 9 weeks. The experimental animals were kept in the pens following a completely randomized design. They were provided the diets adequate for grower stage as recommended by NRC (ME:3,265 kcal/kg and CP:16%). COP fed pigs showed lower weight gain up to 6 weeks of age compared to NC group and other groups without significant differences (P>0.05). The carcass weights of pigs fed VA and COP were significantly higher compared to NC group (P<0.05), Back fat thicknesses groups fed three different additives were higher than NC group and lower then PC group (P<0.05). Crude fat contents in loin meat were significantly lower in groups fed three different additives while moisture contents of those three groups were higher than other groups (P<0.05). The thiobarbituric acid reaction substance (TBARS) value measured at fresh and $2^{nd}$ weeks was lower in additives fed groups but no statistical differences were observed among the treatments (P>0.05). Significantly highest PUFA (16.42g/100g) and ${\omega}$-3 fatty acids (ALA, EPA and DHA) content of meat were observed in COP fed pigs compared to NC group (P>0.05), which might mean that three additives function to enhance serum IgG in pigs. In consequence, it can be suggested that AC, VA and COP may have a potential to replace antibiotics as growth promoter and immune enhancer in the diets for growing pigs.

• 한국식품과학회지
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• 제36권3호
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• pp.465-471
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• 2004

본 실험은 당뇨병 치료에 대한 연구로 우리나라에서 민간요법에 이용되어 오던 식물인 택사에서 혈당강하에 영향을 미친 butanol분획물과 항산화영양소인 비타민 E를 21일간 경구투여 하여 글리코겐, 지질함량 및 지질과산화에 미치는 영향을 검토하였다. 간, 신장 및 폐의 상대적 무게는 당뇨대조군이 정상군에 비해 유의적으로 높은 경향을 보였고 특히 신장은 현저한 비대현상을 나타냈다. 간장 글리코겐 함량은 당뇨대조군에 비해 Ac-VE투여군과 VE투여군에서, 근육의 글리코겐 함량은 VE투여군에서 유의적으로 증가하였다. 간장 콜레스테롤 함량은 당뇨대조군에 비해 Ac투여군과 VE투여군에서 감소하였다. 간장 중성지방 함량은 정상군에 비해 당뇨대조군에서 유의적으로 감소하였으며 당뇨대조군에 비해 모든 당뇨실험군에서 증가하였으며 VE투여군에서 유의적으로 증가하였다. 간장의 MDA 함량은 당뇨대조군에 비해 당뇨실험관에서 감소하였으며 Ac투여군과 Ac-VE투여군에서는 유의적으로 감소하였다. 폐의 경우는 당뇨대조군에 비해 모든 당뇨실험군에서 감소하였으나 유의적인 차이는 없었다. 이상의 결과 streptozotocin으로 당뇨를 유발시킨 흰쥐에서 택사의 투여가 간의 산화적 손상을 감소시키므로써 산화적 스트레스 억제효과를 관찰할 수 있었으며, 비타민 E의 투여가 간장 및 근육의 글리코겐 함량을 증가시키고 중성지방함량을 증가시켰다. 택사와 VE의 상관관계는 보이지 않았고 각자 독자적인 기전에 의해서 발생하는 것으로 사료된다. 그리므로 앞으로 각 조직의 지방대사 및 약물대사효소활성에 비치는 영향에 대해 더욱 연구되어져야 할 것이다.

• 한국식품과학회지
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• 제35권4호
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• pp.713-719
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• 2003

본 연구는 220 g 내외의 흰쥐를 이용하여 정상군, 당뇨대조군, 택사투여군(Ac), 택사와 비타민 E 병용투여군(Ac-VE), 비타민 E투여군(VE)으로 구분하여 실험하였다. 21일간 택사의 BuOH 분획물(400 mg/kg B.W.)과 비타민 E(10 mg/kg B.W.)를 단독 혹은 병용투여한 후 혈당과 혈중 지질함량을 분석하였다. 정상군을 제외한 모든 실험군에서 체중이 감소하였으며, 당뇨대조군에 비해 당뇨 실험군의 체중은 높은 경향을 보였다. 혈당 수준은 택사 투여시 실험 21일째에 당뇨대조군에 비해 유의적으로 감소되었다(p<0.05). 인슐린수준은 당뇨유발군간의 유의적인 차이는 없었으나, Ac-VE투여군의 수치가 다른 군에 비해 높았다. 혈중 콜레스테롤 수준은 실험 14일째에는 당뇨대조군에 비해 모든 당뇨실험군이 유의적으로 낮았으며(p<0.05), 실험 21일째에는 Ac투여군에서 유의적으로 낮았다(p<0.05). HDL-cholesterol함량, 중성지방, 동맥경화지수 및 혈장 유리지방산의 수준은 유의적인 차이가 없었다. ALT 및 AST 활성도는 당뇨대조군에 비해 모든 당뇨실험군이 유의적으로 낮았다(p<0.05). 이 결과 택사 및 비타민 E의 투여가 실험 당뇨 쥐의 혈당 감소와 체중감소 완화, 간의 손상 감소에 효과가 있음을 보여주고 있다. 그러나 본 실험기간동안 택사와 비타민 E 병용투여시 상승효과는 보이지 않았다.