Journal of Nutrition and Health

The Korean Nutrition Society (한국영양학회)
권호 표지

The Effect of Each Fraction of Methanol Extract of Alisma canaliculatum on Blood Glucose Levels and Lipid Metabolism in Streptozotocin Induced Diabetic Rats

택사 분획물의 투여가 Streptozotocin 유발 당뇨 흰쥐의 혈당수준과 지질대사에 미치는 영향

  • 임숙자 (덕성여자대학교 자연과학대학 식품영양학과) ;
  • 김승희 (덕성여자대학교 자연과학대학 식품영양학과)
  • Published : 2001.09.01
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Abstract

Diabetes mellitus was induced in male Sprague-Dawley rats weighing 200-245g by injection of streptozotocin(STZ) into the tail vein at a dose of 45mg/kg and were divided into seven groups ; normal, diabetic control, and five experimental groups(fractions of hexane, chloroform(CHCl$_3$), ethylacetate(EtOAc), butanol(BuOH) and water($H_2O$)). The rats of all groups were fed on AIN-93 diet and the five experimental groups were orally administered each fraction for 14 days. The body weight and diet intake were monitored daily. The plasma levels of glucose, insulin, cholesterol, triglyceride, HDL-cholesterol, free fatty acid and aspartate and alanine aminotransferase activities were analyzed. Diabetic rats showed the lower weight gain compared to the normal rats. The plasma glucose levels of the CHCl$_3$, and $H_2O$ fraction groups were significantly lower than the other experimental groups. The plasma insulin level of the CHCl$_3$ fraction group was much higher than that of diabetic control group. The plasma cholesterol levels were increased in all the experimental groups. The groups of hexane, BuOH and H2() fractions showed the lower plasma triglyceride levels compared to diabetic control group. The plasma free fatty acid levels were not significantly different in all groups. HDL-cholesterol levels were definitely higher in hexane, CHCl$_3$ and EtOAc fraction groups than that of diabetic control group. In conclusion, administration of CHCl$_3$ and $H_2O$ fractions of methanol extract of Alisma canaliculatum exhibited hypoglycemic effects in STZ induced diabetic rats, showing the possibility of therapeutic use of Alisma canaliculatum to the diabetes mellitus.

Keywords

References

  1. Metabolic control and diabetic complications. Contemporary issues in endocrinology and metabolism Sherwin,R.S.;Tamborlane,W.V.
  2. Diabetes v.31 Metabolism of cholesterol and plasma triglycerides in non-ketotic diabetes mellitus Abrams,J.J.;Ginberg,H.;Grundy,S.M.
  3. Lipid v.26 Vitamin E and the susceptibility of erythrocytes and reconstituted liposomes to oxidative stress in aged diabetics Urano,S.;Midori,H.H.;Tochihi,N.;Matsuo,M.;Ito,H.
  4. Exp Gerontol v.25 Oxidative stress as a causal factor in differentiation and aging. A unifying Hypothesis Sohal,R.S.;Allen,R.G.
  5. Korean J Pharm v.21 A preliminary study on hypocholesterolemic and hypoglycemic activities of some medicinal plants Choi,J.S.;Chung,H.Y.;Han,S.Y.
  6. SM Pharm Sci v.7 Studies on the physiochemical activities of Commelinae herba extract on the normal and the streptozotocin-induced hyperglycemic rats. Thesis collection Kim,T.H.;Yang,K.S.;Whang,S.H.
  7. Korean J Nutr v.28 no.7 The effects of Koren wild vegetables on blood glucose levels and liver-muscle metabolism of streptozotocin-induced diabetic rats Lim,S.J.;Kim,S.Y.;Lee,J.W.
  8. Diabetes Care v.12 Traditional plant medicines as treatments for diabetes Bailey,C.J.;Day,C.
  9. Vegetables of herb medicine Jung,P.G.
  10. A serise of Chinese herbal medicines of east medical science 15 Science encyclopedia Co.;East medical science(ed.)
  11. Korean J Soc Food Sci v.13 no.5 Effects of Korean wild vegetables on blood glucose levels and energy metabolites in streptozotocin induced diabetic rats Lim,S.J.;Won,S.B.
  12. Diabetes v.30 Dibetes induced with multiple subdiabetogenic doses of streptozotocin Gold,G.;Manning,M.;Heldt,A.;Nowlain,R.;Pettit,J.G.;Grodsky,G.M.
  13. J Clin Invest v.48 Diabetogenic action of streptozotocin:relationship of dose to metabolic reponse Junod,A.;Lambert,A.E.;Stauffacher,W.;Renold,A.E.
  14. J Nutr v.127 Components of the AIN-93 diets as improvements in the AIN-76A diet Reeves,P.G.
  15. Medical laboratory technology and clinical pathology(2th ed.)
  16. In clinical laboratory methods(9th ed.) Bauer,J.D.
  17. Am J Clin Pathol v.28 A colorimetric method the determination of serum glutamic oxalacetic and glutamic pyruvic transaminases Reitman,S.;Frankel,S.
  18. Scandinav J Lab Invest v.12 On the enzymatic determination of blood glucose Rabbo,E.;Terkildsen,T.C.
  19. J Clin Endocrinol Metab v.33 Use of polyethylene glycol to separate free and antibody-bound peptide hormones in radioimmunoassays Desbuquois,B.;Aurbach,G.B.
  20. Clin Chem v.19 Preparation and properties of a cholesterol oxidase from Nocardia sp. and its application to the enzymatic assay of total cholesterol in serum Richmond,W.
  21. J Clin Chem v.20 Enzymatic determination of total serum cholesterol Allain,C.C.;Poon,L.S.;Chan,C.S.G.;Richmond,W.;Paul,C.Fu.
  22. J Clin Chem v.21 Serum triglyceride determined colorimetry with an enzyme that produces hydrogen peroxide Giegel,J.L.;Ham,S.B.;Clema,W.
  23. J Clin Chem v.24 Cholesterol in highdensity lipoprotein: use of $Mg^{2+}$/dextran sulfate in its measurement Finely,P.R.;Schifman,R.B.;Williams,R.J.;Luchti,D.A.
  24. A summary of clinical test(Revised ed.) v.29 Kim,J.C.
  25. PC-STAT.Dept.Food Sci,Univ Georgia Rao,M.;Blane,K.;Zonnenberg,M.
  26. Diabetes v.43 Skeletal muscle vascular transport capacity in diabetic rats Sexton,W.L.
  27. J Biol Chem v.249 Effect of streptozotocin diabetes and insulin treatment on the rate of protein synthesis in tissues of the rat in vivo Pain,V.M;Garlick,P.
  28. J Nutr v.123 Dietary sorbose prevents and improves hyperglycemia in genetically diabetic mice Furuse,M.;Kimura,C.;Mabayo,R.T.;Takahashi,H.;Okumura,J.
  29. Endocrinolgy v.119 Phenylethanolamin N-methyltransferase in the basis of STZ diabetic rats Fisher,K.J.;Stewat,J.K.
  30. Korean J Nutr v.27 Effects of Buckwheat on organ weight, glucose and lipid metabolism in streptozotocin-induced diabetic rats Lee,J.S.;Son,H.S.;Maeng,Y.S.;Chang,Y.K.;Ju,J.S.
  31. Korean J Nutr v.30 Effect of Artemisia Princeps var Orientalis and Circium japonicum var Ussuriense on liver function, body lipid, and bile acid of hyperlipidemic rat Lim,S.S.;Kim,M.H.;Lee,J.H.
  32. Diabetes v.42 Perspectives in diabetes:hyperglycemic pseudohypoxia and diabetic complications Williamson,J.R.;Chang,K.;Frangos,M.;Hasan,K.S.;Ido,Y.;Kawamura,Y.;Nyengaard,J.R.;Enden,M.V.D.;Kilo,C.;Tilton,R.G.
  33. Diabetes v.39 Cholesterol metabolism in alloxan-induced diabetic rabbits O'Meara,N.M.G.;Devery,R.A.M.;Owens,D.;Collins,P.B.;Johnson,A.H.;Tomkin,G.H.
  34. Korean J Nutr v.30 Effects of mushroom protein-bound polysaccharides on blood glucose levels and energy metabolism in streptozotocin-induced diabetic rats Kim,M.W.;Park,M.H.;Kim,K.H.
  35. Metabolism v.22 Plasma triglyceride transport kinetics in diabetes mellitus Nikkila,E.A.;Kekki,M.
  36. J Korean Diabetes Assoc v.10 The relationship between the postprandial HDL subfraction and triglyceride in diabetes mellitus Choi,S.K.;Yoon,K.H.;Yang,I.M.;Kim,J.W.;Kim,Y.S.;Kim,K.W.;Choi,Y.K.;Park,W.K.;Kim,S.W.