• Journal of the Korea Academia-Industrial cooperation Society
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• v.17 no.9
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• pp.322-328
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• 2016

This study investigated the effects of temperature, salinity, and algal diet to find the optimal conditions for 5 days for the mass culture of the tropical tintinnid, Metacylis tropica. This tintinnid had a small, hyaline, and ovoid lorica. The oral diameter, length, and maximum width of the lorica were $36.7{\mu}m$, $49.5{\mu}m$, and $44.5{\mu}m$, respectively. In the temperature experiments, the highest maximum density and population growth rate were observed at $30^{\circ}C$ with 340.7 cells/mL and 1.1/day, respectively. Lower salinities adversely affected the population growth of M. tropica. The maximum density was observed at 33 ppt (840 cells/mL). In the diet experiments, M. tropica fed Isochrysis galbana showed the highest density (413 cells/mL) and population growth rate (1.2/day). As a result, M. tropica is appropriate as a potential prey organism for early fish larvae with smaller mouths because the tintinnid has a relatively small size compared to the rotifer. In addition, the conditions of $30^{\circ}C$, 33 ppt and supplying I. galbana would be effective in the cultivation of M. tropica.

• Korean Journal of Environmental Biology
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• v.17 no.4
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• pp.529-541
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• 1999

In order to elucidate characteristics of water quality, investigation of monthly dynamics of environmental factors and algal populations at major four stations of the mid and lower part in Taechong Reservoir was performed from June 1998 to June 1999. Water temperature, DO and pH were ranged 5.3~27.7$^{\circ}C$, 6.2~13.8 mgO$_2$/1 and 6.4~9.5, respectively. Those were varying as the season changes. Among inorganic nitrogen nutrients, NH$_4$was ranged from 5.5% to 7.2% of NO$_3$and NO$_3$was almost same through the seasons except summer in which it was low. SRP and SRSi were increased in summer when the blue-green algae became dominant. Those were decreased as the cell density of diatom increased when the water temperature dropped. Therefore SRSi was considered to be another important nutrient factor contributing to the increment of biomass of freshwater algae as well as SRP. Average chi-$\alpha$ concentration ranged from s to 12 $\mu$g/1 and in the lower part or the reservoir, the lowest was found. Moreover, there were remarkable increment in summer when TN/TP ratio were decreasing from relationships between TN/TP ratio and chi-$\alpha$ concentration. Annual mean ratio of TN/TP ratio was relatively high as the value was 110, which was relatively high, and it showed that P is the dominant factor in the algal growth. The dynamics of phytoplankton were simply dominated by a few species seasonally. In summer, blue -green algae such as Anabaena, Microcystis and Oscillatoria were dominant and algal bloom of blue -green began from early summer, sustained to late autumn. The average standing crops of A. spiroides v. crassa, M. aeruginosa and O. limosa were ranged 0.3~2.0$\times$10$^4$cells/ml, 6.4$\times$10$^2$~1.0$\times$10$^4$cells/ml and 4.6$\times$10$^3$~1.6$\times$10$^4$cells/ml, respectively. In winter, diatom Stephanodiscus was considered to be an important species whose average standing crops of Stephunodiscus was 4.9$\times$10$^2$cells/ml from November to April of the next year and the highest was 1.3$\times$10$^3$cells/ml in January.

• Journal of the Korean Society of Marine Environment & Safety
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• v.26 no.5
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• pp.523-530
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• 2020

Based on the results of harmful algal blooms (HABs) monitoring by the National Institute of Fisheries Science and local governments, the effects of changes in the marine environment on HABs are described. Since the beginning of HABs monitoring in 1972, they continued to increase from the 1980s to the 1990s. After the largest number of HAB incidents (109) in 1998; the trend declined until the 2010s. Most HABs in the 1970s were caused by diatoms. In the 1980s, coastal dinoflagellates caused HABs; Cochlodinium polykrikoides blooms have been occurring continuously since 1993. There are three HAB species that cause damage to fisheries in Korea. The high-density bloom of Karenia mikimotoi caused mass mortality in shellfish in Jinhae Bay in 1981. Karenia sp. blooms occurring around Tongyeong in 1992 killed aquaculture fish. Since the occurrence of the largest fisheries damage of KRW 76.6 billion in 1995 caused by C. polykrikoides blooms, they have been occurring continuously. The concentration of nutrients in coastal waters was the highest in the 1980s and has declined since the mid-1990s. This reduction in nutrient concentration is a good explanation for the decreasing number of HABs. Since 2016, a summer high water temperature of 30℃ or more has appeared, and the range and scale of C. polykrikoides blooms have been greatly reduced. In 2016, K. mikimotoi blooms occurred around Wando, Jangheung and Goheung and small scale blooms of C. polykrikoides occurred around Yeosu. There were no C. polykrikoides blooms in 2017; however, Alexandrium affine blooms occurred from Yeosu to Tongyeong. There was a small-scale blooms of C. polykrikoides in 2018 compared to those in the previous years. Our results show that reduction in nutrients and the high water temperature owing to climate change are a good explanation for variation in HABs in Korean coastal waters.

• 한국해양학회지
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• v.27 no.4
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• pp.268-276
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• 1992

Multitrophic interactions among gelatinous planktivores, zooplankton, and phytoplankton were in vestigated in Reeves Bay. New York from mid-March to July in 1989 to evaluate the top-down effect by gelatinous macrozooplankton on the Gyrodinium aureolum bloom through cascading tropic interactions. Zooplankton abundances reached maximal density following a decrease in gelatinous macrozooplankton (hydromedusae and scyphomedusae) abundances, and phytoplankton biomass was low at this time. Subsequently, as ctenophore populations increased zooplankton abundances decreased sharply, and the cell concnetration of G. aureolum began to increase. This field observation supports that the top-down control by gelatinous macrozooplankton on grazers, resulting in low grazing pressure on phytoplankton, can cause an algal bloom. The minimal zooplankton grazing measured using /SUP 14/C tracer technique during the bloom period indicated that zooplankton did not prefer G. aureolum as a good source.

• Journal of Microbiology and Biotechnology
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• v.10 no.4
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• pp.455-461
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• 2000

Abstract Spirulina platensis was grown in SWlUe waste to reduce inorganic compowlds and simultaneously produce feed resources. Spirulina platensis prefers nitrogenous compounds in Ibe order: $NH_4^{+}-N>NO_3^{-}-N>simple-N$ such as urea and simple amino acids. It even consumes $NH_4^{+}-N$ first when urea or nitrate are present. Therefore, the content of residual $NH_4^{+}-N$ in Spimlina platensis cultures can be determined by the relative extent of the following processes: (i) algal uptake and assimilation; (ii) ammonia stripping; and (iii) decomposition of urea to NH;-N by urease-positive bacteria. The removal rates of total nitrogen ffild total phosphorus were estimated as an indicator of the treatment effIciency. It was found that Spirulina platensis was able to reduce 70-93% of $P_4^{3-}-P$, 67-93% of inorganic nitrogen, 80-90% of COD, and 37-56% of organic nitrogen in various concentrations of swine waste over 12 days of batch cultivation. The removal of inorganic compounds from swine waste was mainly used for cell growth, however, the organic nitrogen removal was not related to cell growlb. A maximum cell density of 1.52 dry-g/l was maintained with a dilution rate of 0.2l/day in continuous cultivation by adding 30% swine waste. The nitrogen and phosphorus removal rates were correlated to the dilution rates. Based on the amino acid profile, the quality of the proteins in the Spirulina platensis grown in the waste was the same as that in a clean culture.ulture.

• Korean Journal of Ecology and Environment
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• v.45 no.2
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• pp.232-241
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• 2012

The seasonal changes in phytoplankton species composition, standing crops, dominant species, species diversity and physico-chemical characteristics in the Unmun dam were studied, from August, 2009 to April, 2010. The phytoplanktons of a total of 121 taxa were identified, the composition of phytoplankton community was characterized by a green algae and diatoms, and the quantity composition of standing crops was dominated by diatoms and dinophyceae. The diatoms Aulacoseria granulata and blue-green alga Anabaena planktonica in Summer, Peridinium voltzii in Autumn and Asterionella formosa in Winter to Spring were, especially, prominent. The seasonal changes of the biomass varied from 578 cells $mL^{-1}$ to 12,938 cells $mL^{-1}$, and the maximum algal density was observed in April, Asterionells formosa contributed to 88% of the total cell numbers. The species diversity and richness were highest during autumn, and dominance index was highest in the spring season.

• Journal of Korean Society on Water Environment
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• v.33 no.5
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• pp.538-545
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• 2017

The purpose of this study is to investigate growth response and toxigenicity under various temperature and nutritional conditions, in order to understand the physioecological characteristics of Aphanizomenon flos-aquae, which is a bloom-forming cyanobacterium in the Nakdong River. The strain was inoculated into media under combinations of four temperatures (4, 12, 21, $30^{\circ}C$) and three nutrients (modified CB medium, P-depleted CB medium, N-depleted CB medium) for 28 days. The algae-inhibition tests were performed to assess the potential allelopathic effects of the strains' filtrates on the growth of four algae strains (Microcystis aeruginosa, Aulacoseria ambigua f. spiralis, Aphanizomenon flos-aquae, Scenedesmus obliquus). Toxin production of a strain was measured by Enzyme-Linked ImmunoSolbent Assay (ELISA). The optimal growth temperature (Topt) of strains was $19.9^{\circ}C$ ($18.3-21.2^{\circ}C$), and the temperature range for growth was from $-0.3^{\circ}C$ to $34.3^{\circ}C$. Specific growth rate (${\mu}$) in modified CB medium varied from 0.10 to $0.16day^{-1}$, and the maximum growth rate (${\mu}_{max}$) was $0.17day^{-1}$. Although growth curves under N-existed and N-depleted conditions were almost the same, growth under N-depleted condition was relatively slowed (${\mu}=0.09$ to $0.14day^{-1}$), with a decreased maximum cell density. However, growth under the P-depleted condition was restricted for all temperatures, Two stains of Aphanizomenon flos-aquae were confirmed as not producing toxins, because saxitoxin and cylindrospermopsin were not detected by ELISA. The exudates or filtrates from the Aphanizomenon flos-aquae (DGUC003) resulted in significant inhibition of algal growth on the Aulacoseira ambigua f. spiralis (DGUD001) and Aphanizomenon flos-aquae (DGUC001) (p < 0.01).

• Journal of Environmental Science International
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• v.16 no.7
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• pp.799-804
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• 2007

Oil spill caused severe effects on the marine fauna and flora due to direct contact of organisms with the oil and even in regions not directly affected by the spill. This study was conducted to understand the effects of the oil spill accidents and the use of dispersant on the red tide of Cochlodinium polykrikoides. Crude oil produced in Kuwait, bunker-C, kerosene and diesel oil, and a chemical dispersant produced in Korea, were added with a series of 10 ppb to 100 ppm in the f/2-Si medium at $20^{\circ}C$ under a photon flux from cool white fluorescent tubes of $100\;mol\;m^{-2}\;s^{-1}$ in a 14: 10 h L:D cycle for the culture of C. polykrikoides. In low concentrations of ${\leq}$ 1 ppm of examined oils no impact on the growth of C. polykrikoides was recorded, while in high concentration of ${\geq}$ 10 ppm, cell density was significantly decreased with the range of 10 to 80% in comparison with the control. The growth of C. polykrikoides after the addition of the dispersant and the mixtures combined with oils and a dispersant of ${\geq}$ 10 ppm appeared to decrease, whereas the growth of C. polykrikoides exposed to ${\leq}$ 100 ppb showed little serious impact. However, almost all the C. polykrikoides cells were died regardless of a dispersant and combined mixtures within a few days after the addition of high concentrations.

• Journal of Environmental Science International
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• v.11 no.3
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• pp.215-225
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• 2002

In order to evaluate the relationship between geochemical characteristics and benthic facies of the sediments from the Oenaro Island where red tide proliferation is first observed every year including this year, surface and short multiple core sediment samples were analyzed in terms of geochemical and benthic facies variation. The contents of organic carbon, carbonate, and sulfide gas were relatively low. The variation in C/N ratios, which indicate nature of organic carbon, suggested that the organic carbon recorded in the study area is composed of mixtures of marine and terrigenous organic matters. The concentration of minor elements found at the surface and multiple core sediment samples were also low as well as the enrichment factors(Ef) for the seven heavy metals indicated that the sediment of this area is not polluted significantly. The macrobenthic faunal community comprised 61 species, and their mean density was 708 ind./m2. Polychaete worms were major taxa of this benthic community. A crustacean amphipod, Melita sp. was the most abundant species accounted for 20.7% of total abundance, and the small polychaetes such as Heteromastus filiformis, Paralacydonia poradoxa, Magelona japonica and Sigambra tentaculata were the next dominant species. The macrobenthos around the Oenaro Island were more diverse and abundant than that in Gamak Bay. The benthic communities in the study area sustained somewhat different species composition based on the cluster analysis and the MDS ordination. The benthic community health condition at three stations seemed to be unbalanced, and slightly polluted based on the biological index such as BPI and BC. There was no clear relationship between the geochemistry characteristic and the benthic faunal facies attributed by the micro-algal blooms in this coastal area.

• Journal of Korean Society on Water Environment
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• v.27 no.4
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• pp.445-460
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• 2011

The objective of this study was to construct and assess the applicability of the EFDC model for Saemangeum Reservoir as a 3D hydrodynamic and water quality modeling tool that is necessary for the effective management of water quality and establishment of conservation measures. The model grids for both reservoir system only and reservoir-ocean system were created using the most recent survey data to compare the effects of different downstream boundary conditions. The model was applied for the simulations of temperature, salinity, water quality variables including chemical oxygen demand (COD), chlorophyll-a (Chl-a), phosphorus and nitrogen species and algal biomass, and validated using the field data obtained in 2008. Although the model reasonably represented the temporal and spatial variations of the state variables in the reservoir with limited boundary forcing data, the salinity level was underestimated in the middle and upstream of the reservoir when the flow data were used at downstream boundaries; Sinsi and Garyuk Gates. In turn, the error caused to increase the bias of water quality simulations, and inaccurate simulation of density flow regime of river inflow during flood events. It is likely because of the loss of momentum of sea water intrusion at downstream boundaries. In contrast to flow boundary conditions, the mixing between sea water and freshwater was well reproduced when open water boundary condition was applied. Thus, it is required to improve the downstream boundary conditions that can accommodate the real operations of the sluice gates.