• Title/Summary/Keyword: Algal biomass

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Removal of Cd(II) and Pb(II) Ions in water by the Ulva pertusa and Sargassum horneri (Ulva pertusa 및 Sargassum horneri를 이용한 수중 Cd(II) 및 Pb(II) 이온의 제거)

  • 김영하;박미아;박수인;김택제;이기창
    • Journal of Environmental Science International
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    • v.7 no.6
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    • pp.803-809
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    • 1998
  • Heavy metal ions in water were removed using algal biomass as adsorbents. Absorbents were dried for 3 days, ground them by 40~60 mesh and then were swelled in a buffer solution for 1hr. After being packed in the column, commercially available standard solution of Cd(II) and Pb(II) ions were diluted to get the suitable concentration and then it was eluted with the rate of 1mι/min. Heavy metals on the adsorbents were recovered with nitric acid. More amounts of Cd(II) or Pb(II) ions in green algae, Ulva pertusa, than in brown algae, Sargassum horneri, were adsorbed. Pb(II) ion was adsorbed more than Cd(II) ion in both algae. The pH effect of adsorbed amounts of Cd(II), Pb(II) ions on the biomass was shown the following order ; pH 10.5 > 8.5 > 7.0 > 5.5 > 3.5. Recovery ratio of metal ions front algae is shown higher in acidic or neutral conditions than it in alkalic ones. Pb(II) ion is recovered relatively more than Cd(II) ion in our system.

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Design of Ultra-sonication Pre-Treatment System for Microalgae CELL Wall Degradation

  • Yang, Seungyoun;Mariappan, Vinayagam;Won, Dong Chan;Ann, Myungsuk;Lee, Sung Hwa
    • International journal of advanced smart convergence
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    • v.5 no.2
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    • pp.18-23
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    • 2016
  • Cell walls of microalgae consist of a polysaccharide and glycoprotein matrix providing the cells with a formidable defense against its environment. Anaerobic digestion (AD) of microalgae is primarily inhibited by the chemical composition of their cell walls containing biopolymers able to resist bacterial degradation. Adoption of pre-treatments such as thermal, thermal hydrolysis, ultrasound and enzymatic hydrolysis have the potential to remove these inhibitory compounds and enhance biogas yields by degrading the cell wall, and releasing the intracellular algogenic organic matter (AOM). This paper preproposal stage investigated the effect of different pre-treatments on microalgae cell wall, and their impact on the quantity of soluble biomass released in the media and thus on the digestion process yields. This Paper present optimum approach to degradation of the cell wall by ultra-sonication with practical design specification parameter for ultrasound based pretreatment system. As a result of this paper presents, a microalgae system in a wastewater treatment flowsheet for residual nutrient uptake can be justified by processing the waste biomass for energy recovery. As a conclusion on this result, Low energy harvesting technologies and pre-treatment of the algal biomass are required to improve the overall energy balance of this integrated system.

Effect of Light Quality on Growth and Fatty Acid Production in Chlorella vugaris Using Light Emitting Diodes (발광다이오드를 이용한 광파장에 따른 Chlorella vulgaris의 생장과 지방산 생산에 미치는 효과)

  • Kim, Z-Hun;Kim, Dong Keun;Lee, Choul-Gyun
    • Journal of Marine Bioscience and Biotechnology
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    • v.8 no.1
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    • pp.24-29
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    • 2016
  • Microalgae are considered as superior biodiesel producers, because they could effectively produce high amount of lipid with fast growth rate. In this study, Chlorella vulgaris was exposed to various light wavelengths (${\lambda}_{max}$ 470 nm, ${\lambda}_{max}$ 525 nm, and ${\lambda}_{max}$ 660 nm) using light emitting diodes (LEDs) to examine effect of light quality on their growth and fatty acid production in 0.4-L bubble column photobioreactors. Fluorescent lamps were also used as polychromatic light sources (control). From the results, biomass productivity was varied by light wavelength from 0.05 g/L/day to 0.30 g/L/day. Maximum biomass productivity was obtained from red LED among tested ones. We also observed that contents of oleic acid and linolenic acid, which affect biodiesel properties, were significantly changed depending on supplied wavelength. These results indicated that production of algal biomass, and fatty acid content and productivity could be improved or controlled by supplying specific light wavelength.

Marine Algal Assemblages on Artifical Reefs in Jeju-do Before and After Rocky Cleaning and the Growth Pattern of Ecklonia cava with Water Depth (갯닦기 전후 제주도 인공어초의 해조상 및 수심별 감태의 생장양상)

  • Kwak, Cheol-Woo;Chung, Ee-Yung;Gim, Tae-Yeon;Lee, Jong-Hwa;Kim, Young-Sik
    • Journal of Fisheries and Marine Sciences Education
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    • v.26 no.1
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    • pp.34-48
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    • 2014
  • Marine algal assemblages on the artificial reefs at three stations (Haengwon, Geumneung, Pyoseon in Jeju-do) and the growth pattern of Ecklonia cava with water depth were studied before and after rocky cleaning. Nine algal species occurred at three artificial reefs before rocky cleaning in July 2012, however, 19 algal species were found at three artificial reefs after rocky cleaning in July 2013. In particular, 13 of 19 species in 2013 were replaced by different species which were not found in July 2012. Algal biomass rapidly increased in July 2013 after rocky cleaning. The nMDS plot based on the presence and absence data of macro-algal assemblages on the artificial reefs showed that the species compositions between artificial reefs were similar to each other before rocky cleaning operations in July of 2012. However, after rocky cleaning, the species composition of macro-algal assemblages in Haengwon region was similar to that in Pyoseon region while that in Geumneung region was different to those in Haengwon and Pyseon regions in July 2013. It is needed to clarify the suitable water depth for transplantation of perennial Phaeophyta E. cava. According to the data on seasonal changes in total length, total weight, blade length, blade width, blade weight of E. cava, it began to grow rapidly from April and reached to the maximum value in June, and then degeneration of the blade occurred in July and continued to early August. Although the total length of E. cava at 10m water depth was larger than those at 5 m and 15 m water depths, there was no significant difference statistically among water depths by ANOVA test. However, in the values of total weight, blade length, blade width, and blade weight, the growth patterns at 5 m water depth were larger than those at 10 m and 15 m. Thus, it is assumed that the most suitable water depth for transplantation and its maximum growth and effective release of zoospores of E. cava will be 7~10 m water depth.

Species Composition and Biomass of Marine Algal Community in the Vicinity of Yonggwang Nuclear Power Plant on the West Coast of Korea (서해안 영광원자력발전소 주변 해조군집의 종조성과 생물량)

  • KIM Young Hwan;HUH Sung-Hoi
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.31 no.2
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    • pp.186-194
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    • 1998
  • Species composition and biomass of intertidal benthic algae were studied at the coast of Yonggwang Nuclear Power Plant area and its adjacent stations over 4 seasons (October 1995-August 1996), Of 68 species identified, 7 were Cyanophyta, 12 were Chlorophyta, 14 were Phaeophyta and 35 were Rhodophyta. The largest number of algal species (44) was found at Sangnok, the northernmost station of the study area, whereas the smallest number of species (15) was found from Tongho, ca. 13 km north of the power plant site. Number of species showed highest during the spring (44) and minimum was recorded in autumn (28). Biomass per unit area showed maximum in spring ($189.5\;g\;dry\;wt{\cdot}m^{-2}$ in average) and minimum in winter ($107.9\;g\;dry\;wt{\cdot}m^{-2}$ in average). Biomass values exhibited a wide range of variation among the stations, ranging from a low of $22.0\;g\;dry\;wt{\cdot}m^{-2}$ in annual average at Tongho to a high of $295.7g\;dry\;wt{\cdot}m^{-2}$ in average at Sangnok. Dominant species in biomass were Corallina pilulifera, Sargassum thunbergii, Gymnogongrus flabelliformis and Enteromorpha compressa. There have been little variation in the dominant algal species around the power plant site during the past 10 years and also these algae appeared throughout the west coast of Korea with higher frequency.

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Comparison of Biosorption of N, P ions by Zygnema sterile and Lepocinclism textra Biomass under Irradiation Period in High Rate Algae Biomass Reactor (고율 조류 바이오매스 반응기에서 조사시간으로 본 Zygnema sterile과 Lepocinclism textra 바이오매스의 질소, 인 이온 생흡착의 비교)

  • Kong, Surk-Key
    • Journal of environmental and Sanitary engineering
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    • v.22 no.4
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    • pp.11-21
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    • 2007
  • The recent investigation indicates that the kinetic constants for anionic ions were merely the result of ion exchange between the algae cell wall surface and the anionic ion. In this study, Zygnema sterile and Lepocinclism textra, floating flagellate alga as the dominant algae strains, were cultivated using HRABR(High Rate Algae Biomass Reactor) and the cultivation conditions were 24 hrs. and 12 hrs. irradiation and it was studied how this algal biomass acts on the biosorption mechanism of anionic N and P. Results are as follows : 1. Calculating the specific chl.-a growth rate using Michaelis-Menten model, the one of 24hrs. irradiation was about 55 times higher than the one of 12 hrs. irradiation 2. Calculating the specific chl.-a growth rate using Kuo model, the one of 24 hrs. irradiation was about 2.26 times higher than the one of 12 hrs. irradiation 3. Langmuir model can apply to the biosorption mechanism of anionic N and P in HRABP. 4. Regarding the chlorophyll-a concentration as unit weight of sorbent, the ion selectivity coefficients for N and P are as follows : $(NH_3-N)+(NO_3-N)$ in 24 hrs. irradiation ; 44.984 $PO_4-P$ in 24 hrs. irradiation ; 24.237 $(NH_3-N)+(NO_3-N)$ in 12 hrs. irradiation ; 1432.851 $PO_4-P$ in 12 hrs. irradiation ; 599.076

Optimization of Phototrophic Growth and Lipid Production of a Newly Isolated Microalga, Desmodesmus sp. KAERI-NJ5

  • Joe, Min-Ho;Kim, Dong-Ho;Choi, Dae Seong;Bai, Suk
    • Microbiology and Biotechnology Letters
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    • v.46 no.4
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    • pp.377-389
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    • 2018
  • In this study, a novel microalgal strain, Desmodesmus sp. KAERI-NJ5, was isolated, identified, and evaluated as a candidate for biodiesel feedstock. In a preliminary study, the effects of four general microalgal growth factors, including temperature, pH, light intensity, and concentration of nitrogen source ($KNO_3$), on the microalgal photoautotrophic growth were evaluated. With the exception of light intensity, the growth factors needed to be optimized for the microalgal biomass production. Optimization was done using response surface methodology. The optimal conditions for biomass production were pH 6.54, $27.66^{\circ}C$, and 0.52 g/l $KNO_3$. The biomass production at the optimal conditions was 1.55 g/l, which correlated well with the predicted value of 1.5 g/l. The total lipid and fatty acid methyl ester contents of the cells grown at the optimal conditions were 49% and 21.2% of cell dry weight, respectively. To increase the lipid content of the biomass, microalgae were challenged by nitrogen starvation. Enhancement of total lipid and fatty acid content up to 52.02% and 49%, respectively, were observed. Lipid analysis of the nitrogen-starved cells revealed that C16 and C18 species accounted for 95.9% of the total fatty acids. Among them, palmitic acid (46.17%) and oleic acid (39.43%) dominantly constituted the algal fatty acids. These results suggest Desmodesmus sp. KAERI-NJ5 as a promising feedstock for biodiesel production.

Seasonal Variations in Species Composition and Biomass of Epiphytic Algal Community in an Eelgrass (Zostera marina) Bed (잘피밭에 서식하는 부착해조류 군집의 종조성 및 계절변동)

  • Kwak, Seok-Nam
    • Journal of the Korean Society of Marine Environment & Safety
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    • v.15 no.3
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    • pp.173-177
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    • 2009
  • A total of 3 epiphytic macroalgae were collected from eelgrass bed in Jindong Bay, and Scytosiphon lomentaria and Colpomenia sp. in Phaephyta, Gracilaria sp. in Rhodophyta occurred during study periods. For epiphytic microalgae (diatoms), Cocconeis scutellum and Cocconeis placentula were common species. Seasonal variations of epiphytic algal biomass were marked: the higher epiphytic macroalgae was 3.3 g $DW/m^2$ in November 2003; whereas epiphytic diatoms were 43,153 $cells/m^2$ in June 2003. Diversity and number of epiphytic macroalgae species were the lowest in the study area, compared with those of in other areas such as Koongyang Bay, Dongdae Bay, and Aenggang Bay. These results were therefore likely due to the severe physical characteristics of the intertidal mudflat eelgrass biological characteristics, and the deterioration of water quality.

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Polypropylene Bundle Attached Multilayered Stigeoclonium Biofilms Cultivated in Untreated Sewage Generate High Biomass and Lipid Productivity

  • Kim, Byung-Hyuk;Kim, Dong-Ho;Choi, Jung-Woon;Kang, Zion;Cho, Dae-Hyun;Kim, Ji-Young;Oh, Hee-Mock;Kim, Hee-Sik
    • Journal of Microbiology and Biotechnology
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    • v.25 no.9
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    • pp.1547-1554
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    • 2015
  • The potential of microalgae biofuel has not been realized because of the low productivity and high costs associated with the current cultivation systems. In this study, a new low-cost and transparent attachment material was tested for cultivation of a filamentous algal strain, Stigeoclonium sp., isolated from wastewater. Initially, the different materials tested for Stigeoclonium cultivation in untreated wastewater were nylon mesh, polyethylene mesh, polypropylene bundle (PB), polycarbonate plate, and viscose rayon. Among the materials tested, PB led to a firm attachment, high biomass (53.22 g/m2, dry cell weight), and total lipid yield (5.8 g/m2) with no perceivable change in FAME profile. The Stigeoclonium-dominated biofilm consisted of bacteria and extracellular polysaccharide, which helped in biofilm formation and for effective wastewater treatment (viz., removal efficiency of total nitrogen and total phosphorus corresponded to ~38% and ~90%, respectively). PB also demonstrated high yields under multilayered cultivation in a single reactor treating wastewater. Hence, this system has several advantages over traditional suspended and attached systems, with possibility of increasing areal productivity three times using Stigeoclonium sp. Therefore, multilayered attached growth algal cultivation systems seem to be the future cultivation model for large-scale biodiesel production and wastewater treatment.

Relative Immigration Activity of the Hibernal Diatom Community in Eutrophic Waters (저온기 부영양 수계 규조군집의 유입능)

  • Yoon, Sung-Ae;Kim, Nan-Young;Kim, Baik-Ho;Hwang, Soon-Jin
    • Korean Journal of Ecology and Environment
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    • v.41 no.3
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    • pp.311-319
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    • 2008
  • To understand the relative immigration activities (RIA) of hibernal diatom community in Lake Ilgam (at the station with and without macrophyte) and River Han (at the station with different current and nutrient), a natural and artificial bed substrate was deposited from 15 to 29 January 2008. For the study period, both waters comprised huge biomass of diatom, over 75% of total abundance. Nutrients in water column, ash-free dry matter (AFDM) and chlorophyll-a (Chl-a) concentration in substrates were decreased with increasing of current in rivers, while in lakes the macrophyte largely affected the diatom community and water quality. Algal RIA in rivers was high at stations with high concentration of nutrient, AFDM and Chl-a, and in high nutrients and no macrophytes in lakes, respectively. The species showing a higher RIA was Nitzschia amphibia in rivers and Aulacoseira italica in lakes, while the dominant species were Navicula sp. in rivers and Syndera acus in lakes, suggesting no relationship between water algal biomass and its RIA. These results suggest that the RIA of stream diatom community depends exclusively on the current, while the planktonic diatom in lake was of the macrophyte.