• 약학회지
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• 제19권1호
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• pp.36-46
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• 1975

In the rats administered mercuric chloride (1mg/kg/48 hrs i.p.), it was found that LDH$_{1}$ and LDH$_{2}$ were increased in heart, brain and kidney as well as LDH$_{5}$ increased in liver and muscle in the duration of sixteen days. After the sixteen days of administration, the LDH isozyme patterns in the above mentioned organs were found to be irregularly changed. Considerable amount of mercury accumulation in liver and kidney were found, and especially the mercury accumulation in kidney was notable.

• Maxillofacial Plastic and Reconstructive Surgery
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• 제12권1호
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• pp.41-61
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• 1990

This study was undertaken to investigate the effect of indomethacin on the distribution of Langerhans cells and T-lymphocytes related with immune response of 4-Nitroquinoline-1-oxide induced carcinogenesis at the palate and tongue of albino rat. 54 Sprague-Dawley strain 10 weeks old albino rats, about 150gm weighted, divided into a normal group of 6 rats without treatment, a control group of 12 rats given indomethacin, a carcinogenesis group of 18 whose palatal mucosa were appiled with 4-Nitroquinoline-1-oxide three times a week, and experimental group of 18 rats were treated with indomethacin and whose palatal mucosa were applied 4-Nitroquinoline-1-oxide. All these 54 rats were subjected to be observed as being ATPase stained specimens, specimens for the observation of light and electron microscope, and T-lymphocyte stained specimens. The obtained results were summarized as follows; 1. In carcinogenesis group, proliferation of epithelial layer and rete peg were observed early period of the experiment and showed parakeratosis, individual cell keratinization, acanthosis, and lymphocyte infiltration from 13th week of the experiment on lightmicroscopically, while experimental group showed less reaction than that of carcinagenesis group. 2. The number of Langerhans cells in normal group rarely changed until 21st week of the experiment, while the Langerhans cells increased markedly from 3rd week of the experiment in control group. 3. The number of Langerhans cells were decreased markedly and persistantly until 21st week of the experiment both in carcinogenesis and experimental groups. 4. Appearance of the T-helper cells and T-suppressor cells were minimal and irregullar in number both in normal and control groups. Thus it is assumed that administration of indomethacin and distribution of Langerhans cells showed close relation. 5. In carcinogenesis and experimental groups, the number of the T-helper cells was apparently inereased than that of the T-suppressor cells, but increasing pattern in experimental group was less than in carcinogenesis group. These cells increased most in the 21st week, decreased from the 23rd week and the appearance of these cells were irregular in general throughout the experiment.

• Parasites, Hosts and Diseases
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• 제23권1호
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• pp.41-46
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• 1985

Fibricola seoulensis 감염에 대한 praziquantel의 치료효과를 횐쥐 실험감염을 통하여 관찰하였다. 피낭유충은 뱀의 내장으로부터 분리한 것을 사용하였고 총 51마리의 횐쥐에 각각 500개 또는 1,000개씩을 감염시켰다. 감염 1주 또는 4주후에 praziquantel 1,5,10 또는 20mg/kg 용량을 투여하고 3일후 및 10∼120분후에 흰쥐를 회생시킨 다음 소장으로부터 충체를 회수하였다. 투약 3일후 충체회수율은 치료하지 않은 대조군의 51.3%에 비해 매우 낮아 1mg/kg군의 경우 10.7%, 5∼20mg/kg군의 경우 0.03∼0.%로 나타났다. 따라서 횐쥐의 윤. seouEensis감염을 치료하기 위한 praziquantel의 최소 유효투여량은 5mg/kg인 것으로 생각되었다. 투약후 횐쥐 소장 부위별 충체 분포상황을 관찰함으로써 충체가 배출되는 과정의 일부를 확인하고자 한 바 투약 10분후 이미 많은 충체가 정상 기생부위인 십이지장에서 공장으로 밀려내려간 것이 관찰되었고, 60∼120분후에는 대부분의 충체가 회장 및 맹장에서 회수되었다.

• Parasites, Hosts and Diseases
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• 제33권1호
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• pp.37-44
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• 1995

서울주걱흡충(Neodiplostomum seoulensis)재감염시 숙주에 미치는 영향을 알아보기 위해 충체 회수율 소장의 조직병리 및 미소응모막효소 활성도를 관찰하였다. 자연감염된 뱀에서 피낭유충을 얻어 500개씩 흰쥐에 감염시키고 20일 후에 프라지콴텔로 치료하였다. 치료후 3주째에 피낭유충 500개씩 경구투여하여 재감염 시킨다음 3일. 1주. 2주 후에 도살하여 일차감염군과 비교하였다. 충체회수율은 일차감염시 각각 32.8%. 59.2%, 41.5%에서 재감염시 25.9%, 35.7%. 7.6%로 감소되었는데 특히 2주후에 급격히 감소되었다. 십이지장의 조직병리 소견은 일차감염시 1주 후부 터 융모의 응합 단축등의 퇴행성 변화와. 선와의 증식. 기질층의 염증세포 침윤 등이 나타나 2주까지 지속되었으나 재감염시는 3일 후부터 병변이 나타나다가 1주째부터 회복되었다. 미소응모막효소 활성도 측정결과 십이지장에서 일차감염시 alkaline phosphatase와 자당분해효소의 활성도가 대조군의 절반수준으로 감소하였으나 재감염시에는 대조군 수준과 동일하거나 오히려 증가하였다. 이상의 결과로 이 흡충의 재감염시 충체의 배출이 빨라짐과 동시에 형태학적으로 회복이 빨리 이루어지며 기능적으로도 소장의 미소응모막효소 활성도의 회복이 빠르게 이루어짐을 알 수 있었다.

• Natural Product Sciences
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• 제14권2호
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• pp.95-99
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• 2008

In a project to study the hepatroprotective effect of some plant extracts four plants Ephedra foliate Boiss, Alhagi maurorum Medikus, Capsella bursa-pastoris (L.) Medik. and Hibiscus sabdariffa L. were studied. The ethanol extract of the aerial part of the first three plants and the flowers of H. sabdariffa were subjected to hepatoprotective assays using Wistar albino rats. Liver injury induced in rats using carbon tetrachloride. The biochemical parameters; serum glutamate oxaloacetate transaminase (SGOT), serum glutamate pyruvate transaminase (SGPT), alkaline phosphatase (ALP) and total bilirubin were estimated as reflection of the liver condition. Based on the good results of the biochemical parameters measurements, histopathological study was performed on the liver of rats treated with E. foliate. The normal appearance of hepatocytes indicated a good protection of the extract from carbon tetrachloride hepatotoxicity. All the results were compared with silymarin, the reference hepatoprotective drug.

• Journal of Nutrition and Health
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• 제15권2호
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• pp.91-97
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• 1982

In order to study the effects of commercial fermented milk and Lactobacillus juice on the growth and on blood composition of albino rats, these beverages were administered orally everyday and compared with the control rats. Experimental rats gained weight significantly compare with the control rats within the first week, but the body weights were comparable with the control during the next three weeks. WBC count was increased slightly in the experimental groups, but RBC count, hemoglobin and total serum protein contents were almost the same as that of the control group during the period. Fermented milk was effective in food efficiency ratio and fermented milk and lactobacillus juice were not influenced on any of internal organs weight.

• 한국환경농학회지
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• 제31권3호
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• pp.264-270
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• 2012

본 연구에서는 포공영의 에탄올 추출물의 중금속 중독에 의한 손상 억제 효과를 측정하였다. 현대 사회에서 환경으로부터 오염이 우려되는 납과 수은을 실험동물에 노출시켰고, 납과 수은에 노출된 흰쥐에서 포공영이 이러한 중금속 노출로 영향을 받는 체중변화, 간 및 신장에 미치는 영향을 혈액세포분석 및 혈액 내의 생화학적 분자에 대한 조사하였다. 실험 결과, 포공영을 투여 받은 실험군은 중금속 투여로 인하여 발생하는 체중감소의 개선효과가 나타났고, 혈액세포의 변화에 있어서도 중금속 노출로 감소되었던 RBC의 수를 증가시켰고, 증가된 WBC 및 platelet의 수를 정상수준으로 회복시켰다. 간 및 신장의 손상 지표들로 측정한 Bilirubin, ALP, GOT, GPT, LDH, ceatinine 및 BUN에 있어서도 중금속 노출로 증가된 수치를 농도의존적으로 감소시켰다. 또한 혈액내에서의 중금속 잔존량을 측정한 결과, 포공영 추출물 투여로 인하여 농도의존적으로 그 잔존량이 감소하였다. 따라서, 포공영 에탄올 추출물은 체내 노출된 납과 수은에 대한 독성을 완화시켜주는 것으로 사료된다.

• Applied Microscopy
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• 제26권2호
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• pp.197-206
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• 1996

cis-Dichlorodiammineplatinum (II) (cis-Platin) inhibits the proliferation and growth of the tumor cells by way of inhibiting DNA and protein synthesis of the cancer cells. Although cis-Platin is very effective antitumor drug, it also produces many other side effects. Thus the author has studied the effects of cis-Platin on the proximal epiphyseal plate in the tibia of the rat. The results were as follows: In the chondrocyte of the proliferative zone, sacculated, and fragmented cisternae of rough endoplasmic reticulum, some mitochondria with disorganized mitochondrial cristae and distorted procollagens were observed, and in the matrix some large matrix granules and dispersed collagen fibrils were revealed on the 1st, 3rd day and 1st week group of cis-Platin treated rats. In the chondrocyte of the proliferative zone of cis-Platin treated rats on the 2nd and 3rd week group, parallely arranged rough endoplasmic reticulum and many procollagens were shown, and in the matrix a number of large matrix granules and many small matrical granules as well as collagen fibrils were revealed. Consequently it is suggested that though cis-Platin induces the degenerative changes of the chondrocyte resulting in components of the cartilagenous matrix, these toxic effects are regressed with time.

• Advances in Traditional Medicine
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• 제7권2호
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• pp.141-149
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• 2007

Madhuca indica has been used ethnomedically in Indian folks. In the present study we have investigated anti-inflammatory, anti-ulcer and hypoglycaemic effect of ethanolic extract (EE) and crude alkaloid extract of Madhuca indica seed cake on albino rats. The study showed that the EE had a significant, dose dependent anti-edematogenic, anti-ulcerogenic and hypoglycaemic activity, whereas the crude alkaloid extract exhibited a significant only. Both the extracts possess dose dependent inhibitory activity on carrageenan-induced edema, inhibiting prostaglandins or mediators involved in prostaglandin synthesis, the second phase of inflammation. The EE was significantly effective in protecting pylorus-ligation-induced gastric ulcers at a higher dose level. The active principle of EE seems to be a selective inhibitor of the COX II (prostaglandin synthesis) without important effect on COX I since, EE exhibited both anti-edematogenic and anti-ulcerogenic effect. The EE was effective in reducing the plasma glucose level in normal albino rats in a dose dependent manner, producing hypoglycaemic effect by stimulating the release of insulin from the ${\beta}-cells$ and/or increasing the uptake of glucose from the plasma.

• Advances in Traditional Medicine
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• 제8권4호
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• pp.423-429
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• 2008

Ayurveda, an ancient System of Indian Medicine, has recommended a number of drugs from indigenous plant sources for the treatment of inflammation. To evaluate the anti-inflammatory and analgesic activities of methanol extract of Triphala were investigated in Wister albino rats and mice. The methanol extract of Triphala were found to encompass substantial anti-inflammatory effect in acute and sub-acute models and analgesic effect. Animal models of carrageenan induced edema and cotton pellet induced granuloma in albino rats were used. Extract of 200 mg/kg shows significantly reduced paw edema. Analgesics activity of Triphala with 200 mg/kg shown by significant reduction of writhing. These report shows to support the use methanol extract of Triphala in relieving inflammation and pain.