• 대한수의학회지
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• 제30권1호
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• pp.59-64
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• 1990

The present study was conducted in order to elucidate the asymptomatic carrier state of dermatophytes on the laboratory rodents in Korea during the period from August 1986 to April 1987. A total of 988 laboratory rodents, comprising 393 albino rats, 285 albino mice, 238 guinea pigs and 72 hamsters, were randomly selected for the isolation of dermatophytes from 6 research institutes and 2 breeding farms. And the mating experiments were performed to identified the perfect states of Trichophyton mextagrophytes isolated from albino rats. Dermatophytes were recovered from 94 of 393(23.9%) albino rats and from 3 of 5 colonies. Isolation rate of each colony was 38.4%, 32.0% and 9.8%, respectively, and the albino rats over 2 months old(28.2%) were higher than below 2 months old(2.9%) in the isolation rate of dermatophytes. Among 94 strains of dermatophytes isolated from albino rats, all the strains were identified T mentagrophytes, except 1 strain of Microsporum gypseum, and its perfect states were Arthroderma $vanbreuseghemii^+$.

• Maxillofacial Plastic and Reconstructive Surgery
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• 제11권1호
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• pp.187-202
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• 1989

This study was undertaken to investigate the effect of indomethacin on prostaglandins in 4-Nitroquinoline-N-Oxide (4-NQO) induced palatal carcinoma of albino rats. 128 Sprague-Dawley strain albino rats-about 100g in body weight-were used in this study, divided into as belows; 1. Normal group (16-albino rats) with no treatment, 2. Control group (16-albino rats) treated with prophylene application onto palatal mucosa 3 times a week. 3. Experimental group I (48-albino rats) treated with 0.5% 4-NQO in prophylene application onto palatal mucosa 3 times a week. 4. Experimental group II (48-albino rats) treated with 0.5% 4-NQO in prophylene application with administered $20{\mu}g/ml$ of indomethacin in drinking water ad. lib. Four animals were sacrificed 7th, 13th, 19th, and 25th week respectively in normal and control group, and 7th, 9th, 11th, 13th, 15th, 17th, 19th, 21st, 23rd, 25th, 27th and 29th week respectively in experimental group I and II at each time. The palatal and lingual tissues were excised and kept frozen at $-70^{\circ}C$. Densitometer scan and Beta-counting counter were used for the thin layer chromatography of the arachidonic acid metabolites. The obtained results were as belows; 1. In normal and control group, there was little change of the arachidonic acid metabolites during experiment period, and the tissue homogenates included prostaglandin $D_2$, 6-keto-prostaglandin $F_{1{\alpha}}$, prostaglandin $E_2$, thromboxane $B_2$, prostaglandin $F_{2{\alpha}}$ in that order of relative abundances. 2. In experimental group I, prostaglandin $D_2$, and prostaglandin $E_2$ were increased, while 6-keto-prostaglandin $F_{1{\alpha}}$ and thromboxane $B_2$ were decreased in relative abundances of arachidonic acid metabolites. And there was little change in prostaglandin $F_{1{\alpha}}$ 3. In experimental group II, prostaglandin $D_2$, and prostaglandin $E_2$ were increased, while 6-keto-prostaglandin $F_{1{\alpha}}$ and thromboxane $B_2$ were decreased in relative abundances of arachidonic acid metabolites. And there was little change in prostaglandin $F_{2{\alpha}}$ also. 4. In the range of increase in prostaglandin $D_2$, and prostaglandin $E_2$, and that of decrease in 6-keto-prostaglandin $F_{1{\alpha}}$ and thromboxane $B_2$, in relative abundances, there was wider in experimental group I than in group II. 5. In the range of increase in prostaglandin $D_2$, and prostaglandin $E_2$, and that of decrease in 6-keto-prostaglandin $F_{1{\alpha}}$ and thromboxane $B_2$, in relative abundances, there was wider in palatal mucosa than in lingual mucosa in experimental group I and II.

• Maxillofacial Plastic and Reconstructive Surgery
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• 제11권1호
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• pp.171-186
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• 1989

This study was undertaken to investigate the effects of indomethacin on 4-nitroquinoline 1-oxide (4NQO) induced palatal carcinoma of albino rats. Sixty albino rats about 100 gms of body weight, 6 weeks old-were used classifying as 1) six albino rats of normal group received no treatment, 2) six albino rats of control group treated with propane 1, 2-diol 3 times a week, 3) twenty four albino rats of experimental group I treated with 0.5% 4NQO in propane 1, 2-diol 3 times a week, 4) twenty four albino rats of experimental group II treated with 0.5% of 4NQO in propane 1, 2-diol 3 times a week and administrated 20${\mu}g/ml$ indomethacin in drinking water ad lib. The animals of normal and control groups were sacrificed 7th, 11th, 15th, 19th, 23rd and 27th week, while those of experimental group I and II were sacrificed 7th, 9th, 11th, 13th, 15th, 17th, 19th, 21st, 23rd, 25th, 27th and 29th week after the experiment. The palatal mucosa was excised and examined grossly, light-microscopically and electron-microscopically. Following results were obtained. 1. In control group, there was no specific difference from normal tissue histopathologically. 2. In group I, hyperkeratosis mild acantosis and dyskeratosis in in 7th week, dysplasia in 11th week and severe acantosis in 19th week were observed, but squamous cell carcinoma not observed until 29th week on light-microscope. 3. In group II, hyperkeratosis, mild acantosis in 9th week, dyskeratosis and dysplasia in 21st week, severe acantosis in 27th week and squamous cell carcinoma in 29th week were observed on light-microscope. 4. In group I, widening of intercellular space in 7th week, increasing of desmosome, giant desmosome and tonofilament in cytoplasm in 9th week, severe widening of intercellular space, increasing of mitochondria and vascular degeneration in 11th week, irregular pattern of cell feature and nucleus and prominent nucleoli in 19th week, and continuity of basal lamina in 29th week were observed on electron-microscope. 5. In group II, mild widening of intercellular space in 9th week, increasing of mitochondria, vascular degeneration and tonofilament in cytoplasm in 13th week, increasing of desmosome and giant desmosome in 15th week, irregular pattern of cell surface and nucleus and prominent nucleoli, and in 21st week continuity of basal lamina were observed on electron-microscope which phenomenon occurred little later than group I. After 21st week, however, severe widening of intercellular space, vascular degeneration and continuity of basal lamina were observed as in group I.

• Advances in Traditional Medicine
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• 제8권2호
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• pp.111-124
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• 2008

In the present study, an attempt has been made to assess whether the effect of benzene extract of Ocimum sanctum leaves on the ultrastructural changes in the epithelial cells of the cauda epididymis, its subsequent recovery in the seminiferous epithelium and fertility of male albino rats. Wistar strain male albino rats were orally administered benzene extract of 250 mg/kg body weight of O. sanctum leaves followed by subsequent recovery maintaining suitable controls for 48 days. Results indicate decrease in the weights of testis, epididymis and seminal vesicles. Other accessory organs were not affected. Total count, cell and nuclei diameters of germ cells and Leydig cells were reduced. Cauda epididymis exhibited significant reduction in epithelial height and nuclei diameter of epithelial cells. Cells showed vacuolization with exhibit of signs of degeneration. Ultra study revealed that, in general, the cauda epididymis was affected and in particular, the principal, clear and basal cells were highly disturbed. Further, there was decrease in the size of lipid droplets, mitochondria, Golgi complex, endoplasmic reticulum and accumulation of lysosomal bodies. Fertility performance test showed no implantation in female rats mated with O. sanctum treated rats. Moreover, their recovery after withdrawal of treatment was observed suggesting that the effect of the treatment is transient and reversible. A recovery period resulted in normal spermatogenesis and fertility, suggesting reversible antispermatogenic and antifertility effects of the plant.

• Journal of Nutrition and Health
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• 제8권3호
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• pp.31-37
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• 1975

The effect of dietary restriction was obserbed in Albino rats. Dietary restriction was at 2 levels: 10%, 20%, 30 males and same number of females of Albino rats, aged $30{\sim}40$ days were devided into following 6 groups, 5 rats each. 1 group; rice standard group 2 group; 10% restriction of rice standard 3 group; 20% restriction of rice standard 4 group; 65% rice+35% chinese cabbage group 5 group; 10% restriction of 65% rice+35% chinese cabbage 6 group; 20% restriction of 65% rice+35% chinese cabbage The rats were kept in individual cage and given 6 different diet for 7 weeks. The results of this study were elucidated as follow. 1. Final body weight was not obserbed any significant differences between non-restriction group and 10% restriction group, but 20% restriction group revealed the decrease of body weight. 2. According to dietary restriction, final organ weight was almost decreased in the restriction group of rice+chinese cabbage, and in rice standard group, there were no significants differences between none-restriction group and 10% restriction group, but organ weight was sharply decreased such as Liver, Heart, Kidney, Adrenal, Spleen in 20% restriction group. Sex organ weight of female was increased but weight of male was decreased in 20% restriction group of rice standard group. 3. The result of biochemical analysis such as nitrogen retention, serum cholesterol, glucose of urine, feces, and serum, and lipid of feces and liver, was not revealed any significant diffences, but in 20% restriction group, urinary glucose and serum glucose levels were somewhat decreased, and liver nitrogen level was increased. 4. Therefore there is no effect on albino rats by 10% restricted diet. It can be concluded that it is possible to restrict the diet within 10%.

• 대한치과의사협회지
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• 제12권7호
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• pp.469-471
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• 1974

The purpose of this investigation was to observe the effect of the excessive administration of cortisone on the mast cells of submaxillary gland of albino rats. The eighteen albino rats weighing about 100gm were divided into five experimental groups (fifteen rats) and one normal group(three rats). Five experimental groups of three rats were treated intramuscularly with 25mg cortisone acetate every 2 hours for 2,4,6,8 and 10 hours. The results were as follows : 1. In the two and four hours-injected rats, the mast cells of the submaxillary gland were similar with those of normal group. 2. In the eight and ten hours-injected rats, the number of the mast cells was markedly decreased and metachromatic granules were tended to be decreased.

• Asian Pacific Journal of Cancer Prevention
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• 제16권1호
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• pp.103-108
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• 2015

Curcumin is widely used as a traditional medicine. This work was aimed to investigate its possible protective effect against chemically induced hepatocellular carcinoma (HCC) in rats. Fifty male albino rats were divided into five groups (n=10, each). The control group received a single dose of normal saline, the diethylnitrosamine (DENA) group received a single intra-peritoneal dose at 200mg/kg body weight, and the 3rd, 4th and 5th groups were given DENA and daily administrated curcunine (CUR) via intra-gastric intubation in doses of 300, 200 and 100 mg/kg b.wt. respectively for 20 weeks. Serum, and liver samples were used for determination of alpha feto-protein (AFP), interleukin-2 (IL-2), interleukine-6 (IL-6), serum liver enzymes (AST, ALT, ALP and GGT) levels as well the activities and gene expression of glutathione peroxidise (GPx), glutathione reductase (GR), catalase (CAT) and super oxide dismutase (SOD). Curcumin significantly lowered the serum levels of AFP, IL-2 and IL-6, ALT, ALT, and malondialdehyde (MDA) as well gene expression of IL-2 and IL-6. In contrast it increased the gene expression and activities of Gpx, GRD, CAT and SOD. The protective effect of CUR against DEN-induced hepatocarcinogenesis in albino rats was proven.

• Natural Product Sciences
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• 제13권1호
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• pp.23-26
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• 2007

Hepatoprotective activity of different extracts of Citrullus colocynthis L. sch. (roots) (cucurbitaceae) was investigated in albino rats by inducing hepatotoxicity with carbon tetrachloride. The alcoholic extract of Citrullus colocynthis sch. 100 mg/kg b.w. has been shown to posses significant hepatoprotective effect by lowering the serum level of transaminases (GPT & GOT), alkaline phosphate (ALP) and bilirubin (P < 0.05 to P < 0.001).

• Advances in Traditional Medicine
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• 제6권4호
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• pp.300-305
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• 2006

Pethidine at the dose level of 0.5 mg and 0.75 mg/100 g body weight administered for 20 days to the cycling albino rats caused decrease in the ovarian weight and its protein content. The ovarian folliculogenesis in treated rats is hampered; as a result the follicles which are at the different stages of growth underwent regression. Therefore, the number of healthy follicles is reduced and atretic follicles increased. The elevated levels of ovarian cholesterol and decreased level of glycogen in the pethidine treated rats indicates the inhibition brought in steroidogenesis, which is dependent on pituitary gonadotrophins.

• 대한수의학회지
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• 제7권2호
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• pp.19-24
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• 1967

Normal Albino rats were received glycerin via subcutaneously and Staphylococcus aureus intravenously. The microorganism was coagulase-positive and non-hemolytic. The rats received glycerin alone showed an acute tubular nephrosis, and the others such as glycer in induced nephrotic rats showed a number of different findings: At the first hours of the bacterial injection, in medulla, the bacterial clumps and inflammatory cell infiltration, and microabscesses with retrogressive changes of proximal convoluted tubulles were observed. The suppurative inflammation was observed in days. Five weeks after the initial innoulation of the organism kidney was shown restoration to a histologically normal cortex. The proliferation of fibrous connective tissue and small numbers of chronic inflammatory cells were observed in the medulla where an acute inflammatory process was enhanced presumably. On the other hand, the Albino rats administered Staphyloceccus aureus alone resulted in n moderate degree of vacuolization in proximal convoluted tubules and a number of casts in the early stage. No, bacterial clumps and microabscesses were observed in the rats.