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A Study on the Production of Artificial Seed and Intermediate culture for Attached Spats of the Chinese Stock of a Scallop, Patinopecten yessoensis (중국산 참가리비, Patinopecten yessoensis의 인공종묘 생산 및 부착치패 중간양성에 관한 연구)

  • Oh, Bong-Se;Lee, Jeong-Yong;Park, Se-Ku;Lee, Chu;Jo, Q-Tae
    • The Korean Journal of Malacology
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    • v.24 no.2
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    • pp.153-159
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    • 2008
  • We investigated artificial mass seed production of a Chinese scallop, Patinopecten yessoensis, in 2004. The GSI(gonad somatic index) of the Chinese scallop, P yessoensis was 17.2 on mid-February, 20.2 on mid-March, while that of Korean scallop, P yessoensis was 6.9 on mid-February, 10.8 on mid-March. Matured 120 females and 350 males were selected for artificial mass production. They were exposed in air for 1 hr at over $20^{\circ}C$, and placed into a spawning tank(20 ton) containing sea water treated with UV radiation at $12^{\circ}C$. We gained a total of 228,000 thousand scallop embryos between March 10th and 15th, and reared larvae at the indoor tank during 25 days. When the mean shell length of larvae reached 250 ${\mu}m$ and they have eye-spots, the number of pre-settling larvae was 47,500 thousand. We gained 1,850 thousand attached scallop spats from two kinds of collectors. Attached spats were reared in indoor tank for different periods from 5 days to 60 days. They were divided into 5 groups according to the length of reared days. Each group of attached spats was moved to intermediate rearing sites at Yangyang fishing port in Gangreung-city for acclimation to ocean environments. The highest survival rate of attached spats was 13.0% shown at the group reared for 12 days, but the significant difference in their growth was not found between the groups. The shell length of artificial attached spats increased from 0.9 ${\mu}m$ on July 10th to 24.7 ${\mu}m$ on December 16th with the survival rate of 85.0% while that of natural attached spats increased from 0.6 ${\mu}m$ on July 10th to 23.9 ${\mu}m$ on December 16th with the survival rate of 85.7%.

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Eco-friendly Control of Whiteflies by Two-Fluid Fogging System with Natural Substances in Greenhouses (이류체 포그시스템 및 천연물을 이용한 친환경적 가루이 방제)

  • Kim, Sung-Eun;Lee, Sang-Don;Lee, Moon-Haeng;Kim, Young-Shik
    • Journal of Bio-Environment Control
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    • v.21 no.2
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    • pp.114-119
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    • 2012
  • We have conducted 4 experiments to develop the most environmental and effective use of the two-fluid fog system to prevent and exterminate whiteflies in tomato cultivation. In particular, these experiments used Vitamini tomatoes grown in stand-alone greenhouses at Buyeo Tomato Experiment Station as subjects. Each experiment utilized the fog system in a different way. The first experiment provided the control group, which was subject to the two-fluid fog system without additional humidity control. In the second experiment, the two-fluid fog system controlled the humidity level to be above 70%. The third and the fourth experiment utilized natural substances, which were 1.5 mg/L of Neem Oil and 2 mg/L of Oleic acid respectively, without additional humidity control. From the first experiment, we could observe that a simple use of the two-fluid fog system decreased the density of whiteflies in the greenhouses. This impact of the fog system on whiteflies was greater in the second experiment. By comparing the first and the second experiment, we concluded that whiteflies are more effectively prevented by maintaining a higher humidity level via the fog system's smaller water droplets that float in the air for longer time than the standard fog system in rather dry condition. In the third and the fourth experiments, the extermination level was 78% and 76.4% respectively, comparing only 53% in the first experiment without the humidity control. Therefore, using the natural substances in addition to the humidity control increases the extermination effectiveness. Considering the similar results from the 3rd and the 4th experiments, Oleic acid has a greater appeal for its lower price. Using the two-fluid fog system to both control the humidity on a daily basis and spray the substances for occasional extermination would reduce labor cost and increase production in an environmental way.

Effect of Delignification Treatment after Autohydrolysis on Yields of Cellulosic Substrates, Lignin Contents, and Enzymatic Hydrolysis (목분(木粉)의 Autohydrolysis후(後) 탈리그닌처리(處理)가 섬유소기질(纖維素基質)의 수율(收率), 리그닌함량(含量)과 효소가수분해(酵素加水分解)에 미치는 영향(影響))

  • Park, Jong-Moon;Ahn, Won-Yung;Shin, Dong-So
    • Journal of the Korean Wood Science and Technology
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    • v.12 no.4
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    • pp.19-30
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    • 1984
  • This experiment was carried out to investigate the effects of autohydrolysis and extraction conditions on the separation of the chemical substances, the extractability of lignin by dioxane, and the yield of reducing sugars from cellulosic substrates by using a commercial cellulase derived from Trichoderma viride. Air-dried wood meals through 0.42mm (40 mesh) screen and retained on 0.25 mm (60 mesh) of Populus alba-glandulosa and Pinus koraiensis were autohydrolyzed with water at $180^{\circ}C$ for 30 and/or 60 minutes in a 6 liter stainless-steel digester with or without 2% 2-naphthol. The hydrothermally-treated wood meals were extracted the lignin with 100%, 90%, 75% and 50% dioxane solutions at $70^{\circ}C$ for 4 hours, respectively. The results obtained were as follows; 1) After autohydrolysis of Populus alba-glandulosa, the yield of wood meals decreased with lengthening the auto hydrolysis time from 30 minutes to 60 minutes and with 2% 2-naphthol addition. In case of Pinus koraiensis, the yield was not affected by 2%, 2-naphthol addition at the autohydrolysis in the digester. 2) After autohydrolysis and lignin extraction of Populus alba-glandulosa, the yield of wood meals decreased with lengthening the autohydrolysis time from 30 minutes to 60 minutes and with 2% 2-naphthol addition. Extraction of 50% dioxane solution was the best solvent for the yield among the solutions of 100%, 90%. 75% and 50% dioxane. In case of Pinus koraiensis, the yield was not affected by 2% 2-naphthol addition and the solution of 90% dioxane was the poorest solvent for the yield. 3) After autohydrolysis and lignin extraction of Populus alba-glandulosa, the Klason lignin content in cellulosic substrates for enzymatic hydrolysis decreased with lengthening the autohydrolysis time from 30 minutes to 60 minutes and with 2% 2-naphthol addition. Klason lignin content was the lowest after extraction by 90% or 75% dioxane solution. The content was also affected by interaction of the three factors-autohydrolysis time, 2% 2-naphthol addition and concentration of dioxane. In case of Pinus koraiensis, the Klason lignin content increased with 2% 2-naphthol addition but was not affected by the concentration of dioxane solution. 4) After autohydrolysis and lignin extraction of Populus alba-glandulosa, the extractable Klason lignin content by extraction increased with lengthening the auto hydrolysis time from 30 minutes to 60 minutes and with 2% 2-naphthol addition. The extractable lignin content was the highest after extraction by 90% or 75% dioxane solution. In case of Pinus koraiensis, the extractable lignin content increased with 2% 2-naphthol addition. Extractions by 100%, 90% and 50% dioxane solutions were more effective for the extraction of Klason lignin than by 75% dioxane solution. 5) After autohydrolysis and lignin extraction of Populus alba-glandulosa, the yield of reducing sugars increased with lengthening the autohydrolysis time from 30 minutes to 60 minutes but was not affected by 2% 2-naphthol addition and the concentration of dioxane. The yield of reducing sugars after enzymatic hydrolysis was slightly higher by extractions with 90%, 75% and 50% dioxane solutions than with 100% dioxane. In case of Pinus koraiensis, the yield of reducing sugars was not affected by 2% 2-naphthol addition but affected by the concentration of dioxane. The yield of reducing sugars was the highest in cellulosic substrates extracted by 100% dioxane solution.

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Evaluation of Image Quality in Micro-CT System Using Constrained Total Variation (TV) Minimization (Micro-CT 시스템에서 제한된 조건의 Total Variation (TV) Minimization을 이용한 영상화질 평가)

  • Jo, Byung-Du;Choi, Jong-Hwa;Kim, Yun-Hwan;Lee, Kyung-Ho;Kim, Dae-Hong;Kim, Hee-Joung
    • Progress in Medical Physics
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    • v.23 no.4
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    • pp.252-260
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    • 2012
  • The reduction of radiation dose from x-ray is a main concern in computed tomography (CT) imaging due to the side-effect of the dose on human body. Recently, the various methods for dose reduction have been studied in CT and one of the method is a iterative reconstruction based on total variation (TV) minimization at few-views data. In this paper, we evaluated the image quality between total variation (TV) minimization algorithm and Feldkam-Davis-kress (FDK) algorithm in micro computed tomography (CT). To evaluate the effect of TV minimization algorithm, we produced a cylindrical phantom including contrast media, water, air inserts. We can acquire maximum 400 projection views per rotation of the x-ray tube and detector. 20, 50, 90, 180 projection data were chosen for evaluating the level of image restoration by TV minimization. The phantom and mouse image reconstructed with FDK algorithm at 400 projection data used as a reference image for comparing with TV minimization and FDK algorithm at few-views. Contrast-to-noise ratio (CNR), Universal quality index (UQI) were used as a image evaluation metric. When projection data are not insufficient, our results show that the image quality of reconstructed with TV minimization is similar to reconstructed image with FDK at 400 view. In the cylindrical phantom study, the CNR of TV image was 5.86, FDK image was 5.65 and FDK-reference was 5.98 at 90-views. The CNR of TV image 0.21 higher than FDK image CNR at 90-views. UQI of TV image was 0.99 and FDK image was 0.81 at 90-views. where, the number of projection is 90, the UQI of TV image 0.18 higher than FDK image at 90-views. In the mouse study UQI of TV image was 0.91, FDK was 0.83 at 90-views. the UQI of TV image 0.08 higher than FDK image at 90-views. In cylindrical phantom image and mouse image study, TV minimization algorithm shows the best performance in artifact reduction and preserving edges at few view data. Therefore, TV minimization can potentially be expected to reduce patient dose in clinics.

Report on the External Audits Conducted by Korean Society of Medical Physics (한국의학물리학회 선형가속기 외부 품질관리 실시 현황보고)

  • Huh, Hyun Do;Cho, Kwang Hwan;Cho, Sam Ju;Choi, Sang Hyoun;Kim, Dong Wook;Hwang, Ui-Jung;Kim, Ki Hwan;Min, Chul Kee;Choi, Tae Jin;Oh, Young Kee;Lee, Seoung Jun;Park, Dahl;Park, Sung-Kwang;Ji, Young Hoon
    • Progress in Medical Physics
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    • v.24 no.4
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    • pp.315-322
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    • 2013
  • The aim of this work is to verify the self-quality assurances in medical institutions in Korea through the external audits by the group of experts and have a mutual discussion of the systematic problems. In order to validate the external audits 30 of 80 medical institutions across the nation were picked out considering the regional distribution and the final 25 institutions applied voluntarily to take part in this work. The basic rules were setup that any information of the participants be kept secrete and the measurements be performed with the dosimetry system already verified through intercomparision. The outputs for 2 or more photon beams, the accuracy of gantry rotation and collimator rotation and the poistional accuracy of MLC movement were measured. The findings for the output measurement showed the differences of -0.8%~4.5%, -0.79%~3.01%, and -0.7%~0.07% with respect to that of the verified dosimetry system for the 6MV, 10MV, and 15MV, respectively. For the reference absorbed dose 8 (16%) of 50 photon beams in 25 medical institutions differed 2.0% or greater from the reference value. The coincidences of Field size with x-ray beam and radiation isocenters of Gantry roration and collimator rotation gave the results of within ${\pm}2$ mm for every institute except 2 institutions. The positional accuracy of MLC movement agreed to within ${\pm}1$ mm for every institute. For the beam qualities of 6 MV photon beams kQ values showed the distribution within 0.4% between maximum and minimum. For the protocols 21 institutions (84%) used absorbed dose to water based protocol while 4 insitutions (16%) used air kerma based one. 22 institutions employed the SSD technique while 3 institutions did the SAD one. External audit plays an important role in discovering the systematic problems of self-performing Quality Assurances and having in depth discussion for mutual complementation. Training experts of international level as well as national support system are required so that both the group of experts of medical physicists and government laboratory could perform together periodical and constant external audits.

The Feasibility Study of MRI-based Radiotherapy Treatment Planning Using Look Up Table (Look Up Table을 이용한 자기공명영상 기반 방사선 치료계획의 타당성 분석 연구)

  • Kim, Shin-Wook;Shin, Hun-Joo;Lee, Young-Kyu;Seo, Jae-Hyuk;Lee, Gi-Woong;Park, Hyeong-Wook;Lee, Jae-Choon;Kim, Ae-Ran;Kim, Ji-Na;Kim, Myong-Ho;Kay, Chul-Seung;Jang, Hong-Seok;Kang, Young-Nam
    • Progress in Medical Physics
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    • v.24 no.4
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    • pp.237-242
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    • 2013
  • In the intracranial regions, an accurate delineation of the target volume has been difficult with only the CT data due to poor soft tissue contrast of CT images. Therefore, the magnetic resonance images (MRI) for the delineation of the target volumes were widely used. To calculate dose distributions with MRI-based RTP, the electron density (ED) mapping concept from the diagnostic CT images and the pseudo CT concept from the MRI were introduced. In this study, the look up table (LUT) from the fifteen patients' diagnostic brain MRI images was created to verify the feasibility of MRI-based RTP. The dose distributions from the MRI-based calculations were compared to the original CT-based calculation. One MRI set has ED information from LUT (lMRI). Another set was generated with voxel values assigned with a homogeneous density of water (wMRI). A simple plan with a single anterior 6MV one portal was applied to the CT, lMRI, and wMRI. Depending on the patient's target geometry for the 3D conformal plan, 6MV photon beams and from two to five gantry portals were used. The differences of the dose distribution and DVH between the lMRI based and CT-based plan were smaller than the wMRI-based plan. The dose difference of wMRI vs. lMRI was measured as 91 cGy vs. 57 cGy at maximum dose, 74 cGt vs. 42 cGy at mean dose, and 94 cGy vs. 53 at minimum dose. The differences of maximum dose, minimum dose, and mean dose of the wMRI-based plan were lower than the lMRI-based plan, because the air cavity was not calculated in the wMRI-based plan. These results prove the feasibility of the lMRI-based planning for brain tumor radiation therapy.

MARGINAL MICROLEAKAGE AND SHEAR BOND STRENGTH OF COMPOSITE RESIN ACCORDING TO TREATMENT METHODS OF ARTIFICIAL SALIVA-CONTAMINATED SURFACE AFTER PRIMING (접착강화제 도포후 인공타액에 오염된 표면의 처리방법에 따른 복합레진의 번연누출과 전단결합강도)

  • Cho, Young-Gon;Ko, Kee-Jong;Lee, Suk-Jong
    • Restorative Dentistry and Endodontics
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    • v.25 no.1
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    • pp.46-55
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    • 2000
  • During bonding procedure of composite resin, the prepared cavity can be contaminated by saliva. In this study, marginal microleakage and shear bond strength of a composite resin to primed enamel and dentin treated with artificial saliva(Taliva$^{(R)}$) were evaluated. For the marginal microleakage test, Class V cavities were prepared in the buccal surfaces of fifty molars. The samples were randomly assigned into 5 groups with 10 samples in each group. Control group was applied with a bonding system (Scotchbond$^{TM}$ Multi-Purpose plus) according to manufacture's directions without saliva contamination. Experimental groups were divided into 4 groups and contaminated with artificial saliva for 30 seconds after priming: Experimental 1 group ; artificial saliva was dried with compressed air only, Experimental 2 group ; artificial saliva was rinsed and dried. Experimental 3 group ; cavities were etched with 35% phosphoric acid for 15 seconds after rinsing and drying artificial saliva. Experimental 4 group ; cavities were etched with 35% phosphoric acid for 15 seconds and primer was reapplied after rinsing and drying artificial saliva. All the cavities were applied a bonding agent and filled with a composite resin (Z-100$^{TM}$). Specimens were immersed in 0.5% basic fuschin dye for 24 hours and embedded in transparent acrylic resin and sectioned buccolingually with diamond wheel saw. Four sections were obtained from one specimen. Degree of marginal leakage was scored under stereomicroscope and their scores were averaged from four sections. The data were analyzed by Kruscal-Wallis test and Fisher's LSD. For the shear bond strength test, the buccal or occlusal surfaces of one hundred molar teeth were ground to expose enamel(n=50) or dentin(n=50) using diamond wheel saw and its surface was smoothed with Lapping and Polishing Machine(South Bay Technology Co., U.S.A.). Samples were divided into 5 groups. Treatment of saliva-contaminated enamel and dentin surfaces was same as the marginal microleakage test and composite resin was bonded via a gelatin capsule. All specimens were stored in distilled water for 48 hours. The shear bond strengths were measured by universal testing machine (AGS-1000 4D, Shimaduzu Co., Japan) with a crosshead speed of 5 mm/minute. Failure mode of fracture sites was examined under stereomicroscope. The data were analyzed by ANOVA and Tukey's studentized range test. The results of this study were as follows : 1. Enamel marginal microleakage showed no significant difference among groups. 2. Dentinal marginal microleakages of control, experimental 2 and 4 groups were lower than those of experimental 1 and 3 groups (p<0.05). 3. The shear bond strength to enamel was the highest value in control group (20.03${\pm}$4.47MPa) and the lowest value in experimental 1 group (13.28${\pm}$6.52MPa). There were significant differences between experimental 1 group and other groups (p<0.05). 4. The shear bond strength to dentin was higher in control group (17.87${\pm}$4.02MPa) and experimental 4 group (16.38${\pm}$3.23MPa) than in other groups, its value was low in experimental 1 group (3.95${\pm}$2.51 MPa) and experimental 2 group (6.72${\pm}$2.26MPa)(p<0.05). 5. Failure mode of fractured site on the enamel showed mostly adhesive failures in experimental 1 and 3 groups. 6. Failure mode of fractured site on the dentin did not show adhesive failures in control group, but showed mostly adhesive failure in experimental groups. As a summary of above results, if the primed tooth surface was contaminated with artificial saliva, primer should be reapplied after re-etching it.

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Mineralogical and Physical Properties of Lime Plaster used in Wall Repair in Temple of Bagan, Myanmar (미얀마 바간지역 사원 벽체 보수에 사용되는 석회 플라스터의 광물학적 및 물리적 특성)

  • Ahn, Sunah;Kim, Eunkyung;Nam, Byeongjik;Hlaing, Chaw Su Su;Kang, Soyeong
    • Journal of the Mineralogical Society of Korea
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    • v.31 no.4
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    • pp.267-275
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    • 2018
  • The purposes of this study were to analyze the mineralogical characteristics of slaked lime used for wall repair of traditional buildings in Bagan, Myanmar and to evaluate the physical properties of lime plaster produced by the same method as Bagan region. In the X-ray diffraction and thermal analysis of the Myanmar slaked lime, portlandite ($Ca(OH)_2$) and brucite ($Mg(OH)_2$) were detected as main constituent minerals, and a carbonate rock mainly composed of dolomite ($CaMg(CO_3)_2$) minerals may be used as a raw material to make slaked lime. The field-emission scanning electron microscope analysis showed that the Myanmar slaked lime was composed of irregularly shaped crystals of $0.5{\mu}m$ or larger and a small amount of $0.1{\mu}m$ of plate - like crystals. The size and uniformity of crystals in Myanmar lime is different from that of Korea slaked lime. This may be attributed to the effect of the mineral composition and the lime hydration method of Myanmar, which produces slurry by immersing the burnt lime in excess water for a long period of time. The compressive strength of the lime plaster in Myanmar resulted in a mean value of $1.13N/mm^2$ for the specimens cured for 28 days. The strength of the specimens with Bale juice was $1.03N/mm^2$, respectively. The lime is an air setting material that exhibits strength through long carbonation process. Therefore, it is necessary to evaluate physical properties according to curing period through long-term curing over 28 days in the future.

The Study on Restoration & Repair of the Seated Stone Statue of Buddha in the Samreoung Valley of Mt. Namsan (경주 남산 삼릉계 석불좌상 보존 및 복원 연구)

  • Jeong, Min Ho;Ji, Sung Jin
    • Korean Journal of Heritage: History & Science
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    • v.43 no.3
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    • pp.242-281
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    • 2010
  • There are a large number of Buddhist cultural relics in Mt. Namsan. The cultural relics carry the spirit of people of Shila who dream of Buddhist Elysium and the establishment of Buddhist nation. In the valley and the top of the mountain and on various rock cliff, stone statues of Buddha and stone pagodas stand in harmony with nature. For that reason, Mt. Namsan is called an open-air museum. And it played an important role in establishing 'The UNESCO World Heritage' status for Gyeongdju in December 2000. But sadly, there are many stone relics that have eroded away and damaged from collapsing in the passage of time. The seated stone statue of Buddha in Samreoung valley of Mt. Namsan is one of them. It was created between the 8th and 9th century, and restored without much care nor extensive historical research in 1923. As a result, The face of the Buddha remained with concrete mortar and its nimbus fallen backward and destroyed. Therefore, restoration and repair as well as creation of a statue environment for the statue were urgent. So we immediately started in restoration and repair. First, through the archaeological excavation around the stone Buddha, we carried the stone Buddha on the original position. In order to restore the statues to its original glory created by the Unified Shila Dynasty, we created a restoration plan in corporation with art historians and historians, then restored the jaw and the damage nimbus. Second, we made the weathering & damage map of the stone Buddha. In order to prevent second damage, we cleaned the surface of contaminants with distilled water. Third, we studied restoration method to prevent artificial damage. We recreated parts of his face and halo. Then each parts of the statue were restored to their original position. In the whole process of restoration, we tried to use traditional techniques.

On Securing Continuity of Long-Term Observational Eddy Flux Data: Field Intercomparison between Open- and Enclosed-Path Gas Analyzers (장기 관측 에디 플럭스 자료의 연속성 확보에 대하여: 개회로 및 봉폐회로 기체분석기의 야외 상호 비교)

  • Kang, Minseok;Kim, Joon;Yang, Hyunyoung;Lim, Jong-Hwan;Chun, Jung-Hwa;Moon, Minkyu
    • Korean Journal of Agricultural and Forest Meteorology
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    • v.21 no.3
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    • pp.135-145
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    • 2019
  • Analysis of a long cycle or a trend of time series data based on a long-term observation would require comparability between data observed in the past and the present. In the present study, we proposed an approach to ensure the compatibility among the instruments used for the long-term observation, which would allow to secure continuity of the data. An open-path gas analyzer (Model LI-7500, LI-COR, Inc., USA) has been used for eddy covariance flux measurement in the Gwangneung deciduous forest for more than 10 years. The open-path gas analyzer was replaced by an enclosed-path gas analyzer (Model EC155, Campbell Scientific, Inc., USA) in July 2015. Before completely replacing the gas analyzer, the carbon dioxide ($CO_2$) and latent heat fluxes were collected using both gas analyzers simultaneously during a five-month period from August to December in 2015. It was found that the $CO_2$ fluxes were not significantly different between the gas analyzers under the condition that the daily mean temperature was higher than $0^{\circ}C$. However, the $CO_2$ flux measured by the open-path gas analyzer was negatively biased (from positive sign, i.e., carbon source, to 0 or negative sign, i.e., carbon neutral or sink) due to the instrument surface heating under the condition that the daily mean temperature was lower than $0^{\circ}C$. Despite applying the frequency response correction associated with tube attenuation of water vapor, the latent heat flux measured by the enclosed-path gas analyzer was on average 9% smaller than that measured by the open-path gas analyzer, which resulted in >20% difference of the sums over the study period. These results indicated that application of the additional air density correction would be needed due to the instrument heat and analysis of the long-term observational flux data would be facilitated by understanding the underestimation tendency of latent heat flux measurements by an enclosed-path gas analyzer.