• 동국한의학연구소논문집
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• 제8권1호
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• pp.1-33
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• 1999

동 서양을 막론하고 21C 의료계 화두로 일컬어지고 있는 면역에 대해 조사하기 위해 동물실험논문들을 중심으로 하여 고찰하고 이를 결과로 앞으로의 연구 방향에 대해 제시해 보려 하였다. 사용된 실험동물로는 백서, 마우스, 가토가 단독 또는 복합적으로 이용되었으며 면역기능 저하의 유도를 위해서는 화학적 면역억제제를 쓰기도 하고 특정질환을 유발시켜 그와 면역과의 상관관계를 밝히기도 한 것으로 조사되었다. 한의학에서는 "부정거사"를 면역에 대한 치료원칙의 근간으로 삼고 있는 만큼 실험에 사용된 약물이나 경혈들은 대체적으로 정기를 보하고 경락의 순행을 원활히 하며 거어(祛瘀), 해독(解毒)하는 성질을 가지고 있음이 조사되었으며 또 한약 단독투여보다는 양약과의 병행투여가 치료효과를 더 높일 수 있음도 조사되었다. 조사결과 이에 대한 구체적인 기준농도의 제시, 약물 및 경혈 선택의 객관적 기준 마련, 특정질환에 대한 면역반응기전의 도출, 약침의 면역실험방법론 등에 대한 깊이 있고 다각적인 연구는 인류가 면역에 대한 질환을 정복하는데 있어서 매우 의미있는 연구과제라 사료된다.

• Journal of Microbiology and Biotechnology
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• 제32권2호
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• pp.238-247
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• 2022

Since the skin covers most surfaces of the body, it is susceptible to damage, which can be fatal depending on the degree of injury to the skin because it defends against external attack and protects internal structures. Various types of artificial skin are being studied for transplantation to repair damaged skin, and recently, the production of replaceable skin using three-dimensional (3D) bioprinting technology has also been investigated. In this study, skin tissue was produced using a 3D bioprinter with human skin cell lines and cells extracted from mouse skin, and the printing conditions were optimized. Gelatin was used as a bioink, and fibrinogen and alginate were used for tissue hardening after printing. Printed skin tissue maintained a survival rate of 90% or more when cultured for 14 days. Culture conditions were established using 8 mM calcium chloride treatment and the skin tissue was exposed to air to optimize epidermal cell differentiation. The skin tissue was cultured for 14 days after differentiation induction by this optimized culture method, and immunofluorescent staining was performed using epidermal cell differentiation markers to investigate whether the epidermal cells had differentiated. After differentiation, loricrin, which is normally found in terminally differentiated epidermal cells, was observed in the cells at the tip of the epidermal layer, and cytokeratin 14 was expressed in the lower cells of the epidermis layer. Collectively, this study may provide optimized conditions for bioprinting and keratinization for three-dimensional skin production.

• 한국의학물리학회지:의학물리
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• 제23권4호
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• pp.252-260
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• 2012

최근 Computed tomography (CT) 조사선량의 인체에 대한 부정적 영향이 부각됨에 따라 선량을 줄이는 연구가 활발히 진행되고 있고, 이로 인하여 소 동물에 관한 연구는 점점 임상전의 연구로서 필수적으로 여겨지고 있다. 최근에는 피폭 선량을 줄일 수 있는 방법으로서 이론적으로 투영 데이터가 충분하지 않을 때 정확하게 영상을 재구성 하는 것이 가능한 Total Variation (TV) minimization 알고리즘이 각광받고 있다. 이에 본 연구에서는 micro-CT (DRGem, Harmony80H series, Korea) 시스템에서 획득한 적은 수의 투영 데이터를 가지고 TV minimization에 기초한 반복적 영상 재구성 알고리즘과 기존의 Feldkamp-Davis-Kress (FDK) 알고리즘을 사용하여 영상을 재구성하고 두 알고리즘의 영상 화질을 비교 및 평가하였다. TV minimization 알고리즘의 효과를 평가하기 위해서, 먼저 서로 다른 농도의 조영제, 물, 공기가 들어있는 원통형 팬톰을 제작하였고, micro-CT를 사용하여 영상을 획득하였다. Tube와 검출기 일회전 당 최대 400개의 투영 데이터를 획득할 수 있으며, TV minimization 알고리즘의 영상 복원의 정도를 평가하기 위해서 20, 50, 90, 180장의 적은 투영 데이터를 추출하였다. 영상 비교평가를 위한 참고 영상(FDK-reference 영상)은 마찬가지로 400개의 투영데이터를 이용하여 FDK 알고리즘으로 재구성하였고, 20, 50, 90, 180장의 투영데이터를 가지고 TV minimization 알고리즘, FDK 알고리즘을 이용하여 재구성한 영상과 FDK-reference 영상의 프로파일, Contrast-to-noise ratio (CNR), Universal quality index(UQI)를 각각 비교평가 하였다. 또한, 소 동물에 관한 연구를 위하여 mouse 영상에 관하여 프로파일과 UQI를 분석하여 비교평가 하였다. 결과적으로 90개의 투영데이터를 사용하여 재구성한 원통형 팬톰 영상을 분석하였을 때, TV minimization 영상(TV-90) 및 FDK 영상(FDK-90)의 CNR과 UQI를 비교하였을 때 FDK-90보다 TV-90에서 CNR이 0.21, UQI가 0.18 증가하였다. 원통형 팬톰 영상과 같은 조건에서 mouse 영상을 사용하였을 때, UQI는 FDK-90보다 TV-90에서 0.08 증가하였다. 결론적으로 본 연구결과는 기존의 micro-CT의 투영 데이터의 사분의 일이 되는 투영데이터를 사용하여 영상을 재구성하여 비교평가 한 결과 투영영상 데이터의 수가 제한되는 경우에 FDK 알고리즘보다 TV minimization 알고리즘이 X-ray 조사시간을 줄임으로서 피폭선량을 줄이는데 효과적으로 기여할 것으로 기대된다. 특히, 조사시간의 단축은 물체의 움직임으로 인한 영상 화질의 저하를 감소시키는데 기여할 것으로 사려된다.

• 대한전자공학회:학술대회논문집
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• 대한전자공학회 2005년도 추계종합학술대회
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• pp.489-492
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• 2005

Even though the blood pressure is one of the most widely used index for the healthcare monitoring of hypertensive and normotensive persons, there is no non-intrusive measurement method which is commercialized until now. Pulse Arrival Time (PAT) is known that it has close relation with the systolic blood pressure (SBP) and arterial stiffness. In this study, SBP estimation methods by non-intrusive measurement of PAT are suggested. For the unconstrained measurement of PAT, the first method used the electrically non contact electrocardiogram (ENC-ECG) technique and the reflective type of Photoplethysmography (PPG) sensor on the computer mouse. In the second method, ENC-ECG and the air pressure sensor in the seat cushion on a chair were measured. The third method used ECG electrodes and PPG sensors on the toilet seat cover. The validation and regression analysis of the relationship of PAT and SBP are summarized. These methods have considerable errors to be used for all people. But these can be applied for each subject after the parameter customization within acceptable error. So, it is feasible for suggested methods to be used for monitoring of SBP in daily life in non-intrusive way when there is personal identification system of each subject.

• 한국수정란이식학회지
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• 제8권2호
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• pp.83-90
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• 1993

To investigate the effect of extracellular matrix proteins on the in vitro development of ethanol-induced parthenogenetic eggs of ICR strain mice, those were cultured in vitro in fibronectin, gelatin, or collagen precoated culture dishes containing 1.5 ml of NaH-C03$_3$-BMOC-3 medium at 37$^{\circ}C$ for 96 hrs. under the atmosphere of 5% $CO_2$ and 95% air. Fibronectin, gelatin, or collagen significantly(P$\pm$1.4, 45.4i1.4, and 44.8$\pm$O.9, respectively. And the diameter of those eggs ranged 104.6$\pm$1.9, 102.8$\pm$2.3, and 103.4$\pm$O.8 $\mu$m, respectively.

• 생약학회지
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• 제25권3호
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• pp.226-232
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• 1994

An antioxidant activity of Gardenia Fruit (Gardenia jasminoides Ellis) which has been used for food coloring was studied. The antioxidant activity was determined by measuring lipid peroxide produced when a mouse liver homogenate was exposed to the air at $37^{\circ}C$, using 2-thiobarbituric acid (TBA). Both water and methanol extracts of Gardenia Fruit showed the antioxidant activity. On solvent fractionation, the antioxidant activity was removed into the ethyl acetate and butanol soluble fractions. And the final water soluble fraction also showed the antioxidant activity in the low concentration, but it promoted the lipid peroxidation in the high concentration. Two compounds (I and II) having the antioxidant activity were isolated from the butanol fraction, and compound I also occurred in the ethyl acetate fraction. The antioxidant activity of compound II was more potent than that of I. By analyzing data for UV, IR and $^1H-NMR$, compounds I and II were identified as geniposide and crocin, respectively.

• 한국의류학회지
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• 제36권9호
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• pp.991-999
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• 2012

The Korean wintertime Onmapsi Campaign recommends wearing underwear, layered clothing, or thermal accessories in an indoor environment of a lower than room-heating condition. We surveyed 132 government officers, about Onmapsi wearing conditions and campaign satisfaction. The term was found to be unfamiliar to them, even if they wore underwear or thermal accessories as participants in the campaign for the wintertime office of low temperature 18-$20^{\circ}C$. They wore thick shirt/pants or cardigans/sweaters; however, underwear or thermal vests was worn relatively less. They wear 2.9 layers on top and 1.7 layers in bottom in average. They have their own methods to keep the body warm, which are using 1) functional thermal clothing, such as turtleneck shirts of heat technology new material, 2) functional thermal accessories, such as warmer and muffler, 3) functional office goods, such as a thermal computer mouse and blanket on the desk. The campaign requires more advertisement, a revision on the details of the proper wintertime indoor environment, and develops the varieties of Onmapsi apparel.

• KSBB Journal
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• 제19권1호
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• pp.67-71
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• 2004

본 연구에서는 염생식물의 하나인 해당화의 항산화효과를 탐색하기 위해 해당화의 dichloromethane과 methanol의 조추출물을 합하여 n-hexane, 15% aq. MeOH, n-BuOH, $H_2O$로써 순차적으로 용매 분획하여 DPPH 라디칼, peroxynitrite의 소거 활성과 지질 과산화 저해 활성을 측정하였다. 그 결과 각각의 실험에서 해당화의 유의적인 항산화 효과가 확인되었으며, 특히, 4개의 분획물들 중 n-BuOH 획분이 가장 뛰어난 항산화 효과가 있는 것으로 나타났다. 따라서 해당화의 항산화 효과는 비교적 극성이 큰 2차 대사산물에 의한 것임을 추측할 수 있으며, 해당화의 많은 생물학적인 활성들 중 peroxynitrite 소거 효과와 마우스 간 균질물을 이용한 지질 과산화물 생성 억제 활성에 관한 보고는 이것이 처음이다.

• 한국가축번식학회지
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• 제17권4호
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• pp.365-373
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• 1994

To investigate the effect of EDTA on the in vitro development of parthenogenetic eggs of ICR strain mice, those were cultured in 35mm culture dishes containing NaHCO3-BMOC-3 medium supplemented with 10, 50, 100, or 500$\mu$M of EDTA at 37$^{\circ}C$ for 96hrs. under the atmosphere of 5% CO2 and 95% air. EDTA supplementation of 10, 50, or 100$\mu$M to medium significantly(P<0.01) increase morula and blastocyst formation rate compared with controls in haploid(19.8, 25.9, 39.0% vs. 0.0%). And compared with 10, or 50$\mu$M of EDTA supplementation, significantly(P<0.01) higher morula and blastocyst formation rate resulted from EDTA supplementatin of 100$\mu$M. Both the nuclear number and diameter of blastocysts developed from parthenogenetic eggs were not affected by the morphological types when they were cultured, or the supplementary concentrations of EDTA. The nuclear number of blastocysts developed from haploid, diploid, and immediately cleavaged eggs was 44.8$\pm$1.2, 45.2$\pm$1.5, and 45.4$\pm$1.8, respectively. And the diameter of those eggs ranged 104.4$\pm$1.8, 104.3$\pm$1.2, and 103.8 1.3$\mu$m, respectively.

• 한국가축번식학회지
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• 제17권4호
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• pp.357-363
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• 1994

To investigate the effect of extracellular matrix proteins on the in vitro development of blastomeres isolated from 2, 4, and 8-cell embryos(termed 1/2, 1/4 and 1/8 blastomeres, respectively) of ICR strain mice, those were cultured in fibronectin, gelatin, or collagen precoated culture dishes containing 1.5ml of NaHCO3-BMOC-3 medium at 37$^{\circ}C$ for 72 hrs, under the atmosphere at 5% CO2. and 95% air. Fibronectin, gelatin, or collagen significantly(P<0.01) increased and blastocyst formation rate compared with controls in 1/2(65.3, 59.2, 60.7% vs. 21.6%), 1/4(63.7, 53.4, 57.1% vs. 26.3%), and 1/8 blastomeres(61.1, 52.3, 53.7% vs. 19.1%). Both the nuclear number(P<0.05) and diameter of blastocysts(P<0.01) developed from balstomeres were significantly affected by the origin of blastomeres. The nuclear number of blastocysts developed from 1/2, 1/4, and 1/8 blastomeres ranged 29.3$\pm$1.6, 24.5$\pm$1.3, and 20..$\pm$1.2, respectively. And the diameter of those blastocysts was 88.3$\pm$2.4, 57.6$\pm$2.1, 39.8$\pm$1.9${\mu}{\textrm}{m}$, respectively.