• Title/Summary/Keyword: Air Chemistry

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Optimizing Conditions for Streptomyces chibaensis J-59 Glucose Isomerase Production Using Response Surface Methodology (반응표면분석에 의한 방선균 Streptomyces chibaensis J-59 포도당 이성화효소의 생산 최적화)

  • Joo, Gil-Jae;Park, Heui-Dong
    • Current Research on Agriculture and Life Sciences
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    • v.14
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    • pp.101-110
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    • 1996
  • Using response surface methodology(RSM), the various conditions(agitation speed, air flow, glucose concentration) in jar fermentor culture were investigated to find the optimum conditions for maximum enzyme production. Central-composite-design was used to control the variable constant in the experiment. The glucose isomerase production of Steptomyces chibaensis J-59 was mostly affected by the air flow rate and glucose concentration. The estimated optimum conditions were as follows: 1% birchwood xylan, 1.5% CSL, 0.1% $MgSO_4{\cdot}7H_2O$, 0.012% $CoCl_2{\cdot}6H_2O$, pH 7.0; air flow, 2.2vvm; agitation speed, 587rpm; glucose concentration, 0.586%. Experimental values(7.43GIU/ml) for the enzyme production obtained from the given optimum conditions had a almost resemblane to response values(7.67GIU/ml) predicted by the RSM. The jar fermentor culture by the RSM produced xylose isomerase about 2.7 times as much as the baffled flask culture.

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The Study of Solid Waste Compost Development for Reclaiming Damage Soil in Forest (산림훼손토양 복원을 위한 부숙토 개발 연구)

  • Na, Seung-Ju;Chang, Ki-Woon;Yang, Hui-Young;Jeon, Han-Ki;Lee, Jong-Jin
    • Journal of the Korea Organic Resources Recycling Association
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    • v.13 no.2
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    • pp.107-120
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    • 2005
  • To study the development of solid waste compost to use sewage sludge and paper mill sludge for reclaiming damage soil in forest, the changes of temperature, moisture, chemical properties, heavy metals and harmful compound during the aerobic decomposition were investigated, and the compost decomposition of final products investigated the round paper chromatography method and G.I(Germination index) value. The results were summarized as follows. Temperature was changed a little during early 5days because of air temperature too low. That was rapidly increased to over $50^{\circ}C$ at 4days after first turning and then decreased gradually fallen to $40{\sim}50^{\circ}C$ at 15days after aerobic decomposition in A and C treatments. The second turning was conducted at 18 days after aerobic decomposition, and then the temperature was little changed. At the compare first with terminal product, The moisture content was decreased all treatments but the change was little in A and B treatments. pH was decreased to below 1 in all treatments. EC was increased to below 5dS/m. The content of total carbon, C/N ratio, $NH_4{^+}-N$ were decreased with 4~7%, below 8 and below 500mg/kg in all treatments, respectively. The content of total nitrogen, $NO_3{^-}-N$, CEC were increased with below 0.5%, below 173mg/kg and over $30cmol^+/kg$ in all treatments, respectively. The content of heavy metals and harmful compound were similar during aerobic decomposition and suited to standard of 가 grade in all treatments. The result of round paper chromatography method and G.I. value, The C treatment concluded well aerobic decomposition. Especially, the G.I. value in C treatment was 64.1 and 66.2 at cabbage and grass, respectively.

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Production of ${\beta}-sitosterol$ by Cell Suspension Culture of Chrysanthemum coronarium L. (쑥갓세포의 현탁배양에 의한 ${\beta}-sitosterol$ 생산)

  • Kim, Hyun-Chul;Chung, Ha-Young;Lee, So-Youn;Chung, Ho-Yong;Kim, You-Jung;Baek, Nam-In;Kim, Soung-Hoon;Choi, Geun-Won;Kim, Dae-Keun;Kwon, Byoung-Mok;Park, Mi-Hyun;Chung, In-Sik
    • Applied Biological Chemistry
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    • v.48 no.4
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    • pp.425-430
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    • 2005
  • [${\beta}-sitosterol$] is a plant sterol that reduces cholesterol levels and inhibits the growth of human prostate and colon cancer cells. Optimal conditions for ${\beta}-sitosterol$ production were examined from cell suspension cultures of Chrysanthemum coronarium L. The callus induction was optimal in MS medium containing 1 mg/l NAA and 1 mg/l BAP. Cell suspension culture was also established from the callus. Optimal ${\beta}-sitosterol$ production was obtained when the cells were cultured at an initial density of 2 mg DCW/l in MS medium containing 1 X sucrose (30 mg/l), 1 X nitrogen (1900 mg/l $KNO_3$, 1650 mg/l $NH_4NO_3$), and 1 X phosphate source (170 mg/l). In cell suspension cultures of C. coronarium L. using shake flasks, the peak content of ${\beta}-sitosterol$ was $150{\mu}g/g$ DCW. In cell suspension cultures of C. coronarium L. using an air-lift bioreactor, the maximum ${\beta}-sitosterol$ content of $143.8{\mu}g/g$ DCW was obtained at an air-flow rate of 100 cc/min.

Analysis of PM2.5 Impact and Human Exposure from Worst-Case of Mt. Baekdu Volcanic Eruption (백두산 분화 Worst-case로 인한 우리나라 초미세먼지(PM2.5) 영향분석 및 노출평가)

  • Park, Jae Eun;Kim, Hyerim;Sunwoo, Young
    • Korean Journal of Remote Sensing
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    • v.36 no.5_4
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    • pp.1267-1276
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    • 2020
  • To quantitatively predict the impacts of large-scale volcanic eruptions of Mt. Baekdu on air quality and damage around the Korean Peninsula, a three-dimensional chemistry-transport modeling system (Weather Research & Forecasting - Sparse Matrix Operation Kernel Emission - Comunity Multi-scale Air Quality) was adopted. A worst-case meteorology scenario was selected to estimate the direct impact on Korea. This study applied the typical worst-case scenarios that are likely to cause significant damage to Korea among worst-case volcanic eruptions of Mt. Baekdu in the past decade (2005~2014) and assumed a massive VEI 4 volcanic eruption on May 16, 2012, to analyze the concentration of PM2.5 caused by the volcanic eruption. The effects of air quality in each region-cities, counties, boroughs-were estimated, and vulnerable areas were derived by conducting an exposure assessment reflecting vulnerable groups. Moreover, the effects of cities, counties, and boroughs were analyzed with a high-resolution scale (9 km × 9 km) to derive vulnerable areas within the regions. As a result of analyzing the typical worst-case volcanic eruptions of Mt. Baekdu, a discrepancy was shown in areas between high PM2.5 concentration, high population density, and where vulnerable groups are concentrated. From the result, PM2.5 peak concentration was about 24,547 ㎍/㎥, which is estimated to be a more serious situation than the eruption of Mt. St. Helensin 1980, which is known for 540 million tons of volcanic ash. Paju, Gimpo, Goyang, Ganghwa, Sancheong, Hadong showed to have a high PM2.5 concentration. Paju appeared to be the most vulnerable area from the exposure assessment. While areas estimated with a high concentration of air pollutants are important, it is also necessary to develop plans and measures considering densely populated areas or areas with high concentrations of susceptible population or vulnerable groups. Also, establishing measures for each vulnerable area by selecting high concentration areas within cities, counties, and boroughs rather than establishing uniform measures for all regions is needed. This study will provide the foundation for developing the standards for disaster declaration and preemptive response systems for volcanic eruptions.

Factors Affecting Sap Exudation of Juglans Mandshurica and Acer Mono : (III) Inje Region in Korea (가래나무 수액와 고로쇠나무의 출수량에 미치는 영향 인자 분석 : (III) 인제 지역)

  • Choi, Won-Sil;Choi, In-Gyu;Park, Mi-Jin
    • Journal of the Korean Wood Science and Technology
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    • v.40 no.6
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    • pp.378-388
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    • 2012
  • This study was carried out to investigate the optimum condition for sap exudation of Juglans mandshurica in comparison with Acer mono Max. trees in a site of Inje-Gun, Gangwon-Do, Korea. Amount of sap exudation, air temperature, relative air humidity and tree diameter at breast height (DBH) were monitored for the period of February 1 through March 31, 2008, and correlation analysis of several factors affecting on sap exudation was carried out. Sap exudation from J. mandshurica initiated on Feb. 28 as the same time in case of A. mono and continued for a month, and enhanced in early March compared to around the middle of March for A. mono. The amount of sap of A. mono was linearly proportional to the diameter at breast height (DBH) but there was no apparent relationship for J. mandshurica. As the amount of sap exudation per tree increased then sap exudation per unit DBH was also linearly proportional in both J. mandshurica and A. mono. The amount of sap exudation per unit DBH of J. manshurica were $0.3{\pm}0.1{\ell}/cm$ on average, which was one third times of $0.9{\pm}0.1{\ell}/cm$ for A. mono. During the days of enhanced sap exudation, the atmospheric conditions such as air temperature and relative humidity around J. manshurica trees were very similar with those of A. mono. The minimum temperature was a significant factor affecting the amount of sap of J. mandshurica whereas the range of temperature was the principal factor for A. mono. In conclusion, the sap of J. mandshurica exudated in atmospheric conditions similar to A. mono, but the amount of sap was affected by different atmospheric factors compared to A. mono.

Numerical Analysis of Unstable Combustion Flows in Normal Injection Supersonic Combustor with a Cavity (공동이 있는 수직 분사 초음속 연소기 내의 불안정 연소유동 해석)

  • Jeong-Yeol Choi;Vigor Yang
    • Proceedings of the Korean Society of Propulsion Engineers Conference
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    • 2003.05a
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    • pp.91-93
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    • 2003
  • A comprehensive numerical study is carried out to investigate for the understanding of the flow evolution and flame development in a supersonic combustor with normal injection of ncumally injecting hydrogen in airsupersonic flows. The formulation treats the complete conservation equations of mass, momentum, energy, and species concentration for a multi-component chemically reacting system. For the numerical simulation of supersonic combustion, multi-species Navier-Stokes equations and detailed chemistry of H2-Air is considered. It also accommodates a finite-rate chemical kinetics mechanism of hydrogen-air combustion GRI-Mech. 2.11[1], which consists of nine species and twenty-five reaction steps. Turbulence closure is achieved by means of a k-two-equation model (2). The governing equations are spatially discretized using a finite-volume approach, and temporally integrated by means of a second-order accurate implicit scheme (3-5).The supersonic combustor consists of a flat channel of 10 cm height and a fuel-injection slit of 0.1 cm width located at 10 cm downstream of the inlet. A cavity of 5 cm height and 20 cm width is installed at 15 cm downstream of the injection slit. A total of 936160 grids are used for the main-combustor flow passage, and 159161 grids for the cavity. The grids are clustered in the flow direction near the fuel injector and cavity, as well as in the vertical direction near the bottom wall. The no-slip and adiabatic conditions are assumed throughout the entire wall boundary. As a specific example, the inflow Mach number is assumed to be 3, and the temperature and pressure are 600 K and 0.1 MPa, respectively. Gaseous hydrogen at a temperature of 151.5 K is injected normal to the wall from a choked injector.A series of calculations were carried out by varying the fuel injection pressure from 0.5 to 1.5MPa. This amounts to changing the fuel mass flow rate or the overall equivalence ratio for different operating regimes. Figure 1 shows the instantaneous temperature fields in the supersonic combustor at four different conditions. The dark blue region represents the hot burned gases. At the fuel injection pressure of 0.5 MPa, the flame is stably anchored, but the flow field exhibits a high-amplitude oscillation. At the fuel injection pressure of 1.0 MPa, the Mach reflection occurs ahead of the injector. The interaction between the incoming air and the injection flow becomes much more complex, and the fuel/air mixing is strongly enhanced. The Mach reflection oscillates and results in a strong fluctuation in the combustor wall pressure. At the fuel injection pressure of 1.5MPa, the flow inside the combustor becomes nearly choked and the Mach reflection is displaced forward. The leading shock wave moves slowly toward the inlet, and eventually causes the combustor-upstart due to the thermal choking. The cavity appears to play a secondary role in driving the flow unsteadiness, in spite of its influence on the fuel/air mixing and flame evolution. Further investigation is necessary on this issue. The present study features detailed resolution of the flow and flame dynamics in the combustor, which was not typically available in most of the previous works. In particular, the oscillatory flow characteristics are captured at a scale sufficient to identify the underlying physical mechanisms. Much of the flow unsteadiness is not related to the cavity, but rather to the intrinsic unsteadiness in the flowfield, as also shown experimentally by Ben-Yakar et al. [6], The interactions between the unsteady flow and flame evolution may cause a large excursion of flow oscillation. The work appears to be the first of its kind in the numerical study of combustion oscillations in a supersonic combustor, although a similar phenomenon was previously reported experimentally. A more comprehensive discussion will be given in the final paper presented at the colloquium.

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Comparison of Sampling Methods for On-Farm Use Quick Test Procedure of Soil Nitrate (토양의 질산태질소 현장검정을 위한 시료 채취방법 비교)

  • Kang, Seong-Soo;Kim, Ki-In;Chung, Keun-Yook;Hong, Soon-Dal
    • Korean Journal of Soil Science and Fertilizer
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    • v.38 no.1
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    • pp.32-37
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    • 2005
  • The procedure of soil sampling for on-farm quick test of soil nitrate is very important to improve practical application without weighing or drying soil. To improve application of test strip reflectometer as a quick on-farm analytical procedure for the estimation of soil nitrate concentration, three sampling methods such as gravimetric sampling (GS), particle density sampling (PDS) and bulk density sampling (BDS) for on-farm analytical procedure were investigated with twelve soils of 45 to $281mg\;kg^{-1}$ nitrate nitrogen concentration. The nitrate nitrogen concentrations measured from different soils were compared with two analytical methods, ion electrode method as a standard laboratory analysis (SLA) and test strip reflectometer at three moisture conditions, viz. air dried soil, 20 and 40% of maximum water holding capacity (MWHC). Nitrate nitrogen concentration measured by test strip reflectometer was significantly correlated with that of SLA, and the coefficients of variation (CV) were in the range of 3.5 to 10.9%. These CV values less than 10.9% were thought to be acceptable for the measurement of soil nitrate as an on-farm real time analytical procedure. The nitrate nitrogen concentration by BDS for test strip reflectometer as well as ion electrode method was more similar to that of SLA compared with those by GS and PDS especially in case of moist soils. This result suggests that the BDS is more useful than GS and PDS in case of on-farm analytical procedure of soil nitrate for moist soils. Further the practical measurement by BDS could be improved by substituting the bottle cap with a larger container.

Change of Residual Deltamethrin Sprayed in the Stream Water according to Wind Speed and Diffusion (풍속 및 수중확산에 따른 방역용 Deltamethrin의 수중 잔류변화)

  • Cho, Kyung-Won;Pak, Jae-Hun;Lim, Jong-Sung;Yoon, Ji-Yeong;Moon, Hye-Ree;Lee, Yong-Ju;Lee, Sung-Kyu;Lee, Kyu-Seung
    • The Korean Journal of Pesticide Science
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    • v.17 no.2
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    • pp.126-132
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    • 2013
  • The objectives of this study were to provide the basic data of the residue of deltamethrin in the stream water. Deltamethrin was treated on side of Ban-Suk stream and Juk-Dong ditch for hygienic purpose by air spray. The drift concentration of deltamethrin was investigated with different wind speed condition on Ban-Suk stream (A), and the change of residue with time course on Juck-dong ford (B). Also we found the residual change of deltamethrin until 48 hour in Yu-Seong stream confluence (C) where two streams join. Maximum residues of A were $0.17{\mu}g/L$ (5 min, 200 m) at strong wind speed and $4.42{\mu}g/L$ (0 min, 25 m) at moderate wind speed according to different wind velocity. Residues of B were $0.15{\sim}0.26{\mu}g/L$ (0~480 min) after spraying, and decreased to a non-detected level after 720 min. Residues of C were $0.15{\mu}g/L$ (0 min), $0.11{\mu}g/L$ (1 min) and $0.10{\mu}g/L$ (12 hr) after spraying, and no residues were detected in any other samples. From these results, it is concluded that deltamethrin residues in water should be rapidly diluted into stream water and affected negligible toxic effect to stream ecosystem.

Composition and emission characteristics of fine particulate matters at the 1100 Site of Mt. Halla during 2011-2012 (한라산 1100고지 대기 미세먼지의 조성 및 배출 특성: 2011~2012년 측정)

  • Song, Jung-Min;Bu, Jun-Oh;Kim, Won-Hyung;Ko, Hee-Jung;Kang, Chang-Hee
    • Analytical Science and Technology
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    • v.29 no.5
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    • pp.209-218
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    • 2016
  • PM10 and PM2.5 samples were collected at the 1100 site of Mt. Halla in Jeju Island during 2011~2012, and their ionic and elemental species were analyzed, in order to investigate the characteristics of emission sources as well as aerosol compositions. The mass concentrations of PM10 and PM2.5 were 22.0±13.1 µg/m3 and 11.3±6.1 µg/m3, respectively, showing 2.4~2.6 times lower than those of the capital city area of Korea. The composition ratios of major secondary pollutants (nss-SO42−, NH4+, and NO3) were the highest as 85.5 % for PM10 and 91.3 % for PM2.5, and followed by the order of marine (Na+, Cl, and Mg2+), organic acid (HCOO and CH3COO), and soil (nss-Ca2+) sources. Among the elemental species in PM10, soil-originated components (Al, Fe, and Ca) were consisted of 50.9 %, which was higher proportion than marine and anthropogenic elements. The acidification of the fine particulate matters was found to be influenced mostly by sulfuric and nitric acids, and these acids were mainly neutralized by calcium carbonate in PM10 and by ammonia in PM2.5. The clustered back trajectories showed that 47 % of total air mass inflows was from the China, and the concentrations of NO3 and nss-Ca2+ were especially high corresponding to the inflows.

Oxidation of Isopropyl Alcohol in Air by a Catalytic Plasma Reactor System (촉매-플라즈마 반응 시스템을 이용한 아이소프로필 알코올 산화)

  • Jo, Jin Oh;Mok, Young Sun
    • Applied Chemistry for Engineering
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    • v.25 no.5
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    • pp.531-537
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    • 2014
  • A catalytic plasma reactor was employed for the oxidation of isopropyl alcohol (IPA) classified as a volatile organic compound (VOC). Copper oxide (Cu : 0.5% (w/w)) supported on a multichannel porous ceramic consisting of ${\alpha}-Al_2O_3$ was used as a catalyst, which was directly exposed to the plasma created in it. The effects of discharge voltage and reaction temperature on the concentrations of IPA and its byproducts were examined to understand the behavior of the catalytic plasma reactor. Without thermal insulation, the reactor temperature increased up to $120^{\circ}C$ at an applied voltage of 17 kV (discharge power : 28 W), and the IPA at a flow rate of $1L\;min^{-1}$ ($O_2$ : 10% (v/v); IPA : 1000 ppm) was completely removed. At temperatures below $120^{\circ}C$, however, besides the desirable product $CO_2$, several unwanted byproducts such as acetone, formaldehyde and CO were also formed from IPA. On the other hand, when the reactor was thermally insulated, the plasma discharge increased the temperature up to $265^{\circ}C$ under the same condition and most of IPA was oxidized to $CO_2$. Without loading CuO on the ceramic support, the plasma discharge in the thermally insulated reactor produced nearly equal amounts of $CO_2$ and CO. On comparison, with the catalyst alone (temperature : $265^{\circ}C$), more than 70% of the removed IPA was simply converted into another type of VOC (acetone), indicating that the catalyst assisted by the plasma is more effective in the oxidation of IPA than that of the catalyst-alone process.