• Molecules and Cells
• /
• v.39 no.8
• /
• pp.594-602
• /
• 2016

Alkyl hydroperoxide reductase subunit C from Pseudomonas aeruginosa PAO1 (PaAhpC) is a member of the 2-Cys peroxiredoxin family. Here, we examined the peroxidase and molecular chaperone functions of PaAhpC using a site-directed mutagenesis approach by substitution of Ser and Thr residues with Cys at positions 78 and 105 located between two catalytic cysteines. Substitution of Ser with Cys at position 78 enhanced the chaperone activity of the mutant (S78C-PaAhpC) by approximately 9-fold compared with that of the wild-type protein (WT-PaAhpC). This increased activity may have been associated with the proportionate increase in the high-molecular-weight (HMW) fraction and enhanced hydrophobicity of S78C-PaAhpC. Homology modeling revealed that mutation of $Ser^{78}$ to $Cys^{78}$ resulted in a more compact decameric structure than that observed in WT-PaAhpC and decreased the atomic distance between the two neighboring sulfur atoms of $Cys^{78}$ in the dimer-dimer interface of S78C-PaAhpC, which could be responsible for the enhanced hydrophobic interaction at the dimer-dimer interface. Furthermore, complementation assays showed that S78C-PaAhpC exhibited greatly improved the heat tolerance, resulting in enhanced1 survival under thermal stress. Thus, addition of Cys at position 78 in PaAhpC modulated the functional shifting of this protein from a peroxidase to a chaperone.

• Journal of Korea Port Economic Association
• /
• v.28 no.2
• /
• pp.179-194
• /
• 2012

In this study, we compared the efficiency of container terminals using DEA. To do this, we designed an AHP/DEA hybrid model using AHP and DEA, and evaluated the efficiency by comparing the container terminal operation company in Gwangyang(KEC, KIT, GICT) and Busan(HBCT, DPCT, KBCT, UPT, Gamman, PNC, PNIT, HJNC, HPNT). The proposed model can control the number of selected promising container terminal by applying DEA-AR model. This model can also improve the credibility of analysis by using objective weights through the AHP application to efficiency evaluation data and normalizing the evaluation data to apply AHP and DEA. The model assumes inputs to be container crane, transfer crane, yard tractor, and reach stacker and output as container traffic. The result shows that DPCT was an efficient DMU.

• Proceedings of the Korean Operations and Management Science Society Conference
• /
• 2004.10a
• /
• pp.47-50
• /
• 2004

웹사이트의 품질 평가는 기본적으로 품질과 관련한 개별 평가요소들에 대한 측정을 필요로 한다. 또 이러한 요소들은 웹사이트 유형에 따라 그 상대적 중요도가 다르기 때문에, 개별 웹사이트 유형에 따라 요소들의 중요도를 정확히 산출하는 것이 성공적 평가의 관건이다. 그러나 웹사이트 평가요소들의 가중치에 대한 합리적 산출방법에 관한 연구는 아직 미흡한 실정이다. 본 연구에서는 평가요소들에 대한 가중치를 AHP기법을 이용하여 합리적으로 도출하기 위하여 인터넷뱅킹 이용자들을 대상으로 개별 평가요소들의 중요도에 대한 인식을 조사하여 분석하였다.

• Journal of the Korea Institute of Information and Communication Engineering
• /
• v.13 no.9
• /
• pp.1737-1744
• /
• 2009

This study calculated the relative importance between evaluation factors of websites focused on culture and tourism in J region by 3C_D_T evaluation model of website. That is, this paper was analyzed by AHP (analytic hierarchy process) and calculated the relative weights and preference. In conclusion, this study showed that content was the most important factor and proposed the improvement planning through sensitivity analysis.

• Journal of Construction Engineering and Project Management
• /
• v.4 no.1
• /
• pp.45-50
• /
• 2014

Productivity analysis is the most important and significant method for evaluating management and engineering performance during whole project stage. However, it is very difficult in developing qualitative index to construction industry comparing to other industries. Therefore, analytical hierarchy process (AHP) is one of the methods for overcoming these limitations by checking consistency index using duality comparison. In this study, it is scraped up an application plan and selection for innovative tools by analyzing survey results on tool users and site managers with respect to using Modified-AHP performance measurement method.

• Korean Management Science Review
• /
• v.13 no.3
• /
• pp.75-90
• /
• 1996

This paper presents an improved method of the Analytic Hierarchy Process (AHP) when customers are to select the best automobile purchase models. In order to support group decisions and as a different procedure of the conventional AHP, we combine the AHP model with a spreadsheet model that applies the Likert's rating scheme to each alternative. We only consider individual pairwise comparison matrices where the consistency ratio (C.R.) is less than or equal to 0.2. Finally, we regard the weight of each decision maker as a reciprocal number of C.R. As a case study we prioritize three passenger cars of medium size in the domestic market. The major evaluation criteria include:exterior or interior features, performance, safety, pricing, salesman, and after service.

• Journal of the Korea Convergence Society
• /
• v.8 no.2
• /
• pp.163-168
• /
• 2017

The feasibility evaluation of public projects has been carried out mainly in the economic analysis. However, items of political, technological and balanced regional development aspects are further evaluated by applying analytic hierarchy process, This paper suggests to analyzes preliminary feasibility study of construction and research & development project during the past three years and to verify correlation between B/C ratio and AHP weight. The result shows that the correlation between cost benefit ratio and the weight of the hierarchy is 0.744 in construction projects and 0.899 in research & development project. Namely, AHP weight be under the control of B/C ratio and is devoted to its role against expectation. Therefore it must be sufficiently discussed in various designs of studies to maximize the effects of AHP analysis.

• Asian-Australasian Journal of Animal Sciences
• /
• v.17 no.3
• /
• pp.355-365
• /
• 2004

Two experiments were conducted to determine the effect of alkaline hydrogen peroxide (AHP) treatment (1% NaOH+1.5% $H_2O_2$; 1 AHPMS, 2% NaOH+1.5% $H_2O_2$; 2AHPMS) on rate and extent of degradation of mustard straw (MS) in sacco in sheep, and its in vivo digestion and ruminal fermentation characteristics when fed to sheep with concentrate (200 g per sheep daily). The treatment of straw with 1 and 2% AHP increased its sodium content by 148 and 296% to that of untreated straw (UMS). There was significant decrease in NDF and hemicellulose contents of AHP treated straw and increase in cellulose and lignin contents. Phenolic acids like ferrulic, $\rho$-coumaric and o-coumaric significantly (p<0.001) reduced by AHP treatment of mustard straw. In first experiment the in sacco degradation of DM, OM and NDF was significantly (p<0.01) greater for 2 AHPMS than for UMS at all incubation periods. The disappearance of nutrient from 1 AHPMS and 2 AHPMS treated straws continue to increase up to 96 h whereas in UMS the peak disappearance was found at 48 h. By using the equation {(y=a+b) ($1-e^{-ct}$)} the degradation rates (c) for DM, OM, and NDF were significantly higher for UMS than AHP treated straws. Level of alkali (1 and 2%) had significant effect on degradation characteristics (a, b, c and $P_{0.05}$) of DM and NDF fraction of MS. However, the effect was not pronounced on OM fraction of MS. In feeding experiment, the intake of nutrients for DM, OM, cell wall constituents and energy was higher on 2 AHPMS, whereas no effect on the digestibility of these nutrients was observed. The apparent nitrogen retention was higher (p<0.05) both in 1 and 2 AHPMS groups. Water intake by animals was significantly increased due to AHP treated mustard straw feeding. Rumen liquor pH was higher in 2 AHPMS fed animals. The $NH_3-N$ of rumen liquor was not affected by feeding of AHP treated MS based diets. Total VFA concentration was significantly (p<0.01) higher in UMS fed group. The fractional out flow rate of DM was higher (p<0.05) in animals fed on 2 AHPMS diets compared to UMS and lAHPMS fed groups. The population of large holotrichs was higher (p<0.05) on AHP treated MS fed diets compared to UMS. The study indicated that treatment of mustard straw with AHP changed its chemical composition towards a better feed. The nutritive value of 2% AHP treated mustard straw was better in terms of dry matter intake and apparent nitrogen retention. The higher in sacco DM, OM and NDF disappearance however, was not confirmed by in vivo data in this study.

• Journal of Korean Society of Forest Science
• /
• v.99 no.1
• /
• pp.24-35
• /
• 2010

This paper aims to contribute to the introduction of forest certification (a private enterprise) in Korea by developing C&I with consideration of the Korean forestry situation. For this study, 6 attributes for establishing certification system at FMU level was applied. To weigh relative importance of international organization's assortment and indicators, AHP survey was conducted to each experts in political, ecological, social, and economic field. By the experts attending FSC's auditing process, indicators adopted through AHP survey were inspected again for the realistic application in the fields. In this process, the typical environment of Korean forestry in real aspect and problems in FSC system were reaffirmed. Therefore, 7 criteria, 27 indicators and 92 checklists were finally developed for the construction of forest certification based on the results of AHP surveys and from investigation on the realistic situation in the fields.

• International Journal of Highway Engineering
• /
• v.8 no.4 s.30
• /
• pp.145-157
• /
• 2006

Generally, the method of the policy analysis has been used for making a policy decision of the development project of the road through the expert interview based on AHP. According to introducing PI, the study of the method of the integrating process for various opinion of the related groups is required. On this study I evaluate what is a difference between the results of integrated process and the AHP analysis on what is major considering factor in decision-making process. From the analysis, each evaluate factor is in level of significance by ANOVA. First, there is a significance level difference between Group A, C and Group B Second, there is also a significance level difference on the result of AHP analysis for each group in order to evaluate by integrated method. Finally, the method of AHP analysis which considers priority factor is expected as to be useful tool on decision-making of various group.