• Journal of Korean Society of Forest Science
• /
• v.99 no.3
• /
• pp.344-352
• /
• 2010

To investigate the mating system of clones in the seed orchard of Japanese red pine, parameters of mating system, such as outcrossing rates, number of potential pollen contributors, and degree of pollen contamination, were estimated in the seed orchard of Japanese red pines on the basis of DNA data including 4 nSSR and 6 cpSSR markers. Estimates of outcrossing rates were ranged from 94.9 to 100% with an average of 98.9% on the basis of the analysis of cpSSR haplotypes. They were ranged from 90.3% to 100% with an average of 95.9% on the basis of the analysis of nSSR genotypes. However, cross checking of both DNA markers revealed that the seeds presumed to be products of self pollination were turned out to be generated by pollination between mother tree and other tree (i.e., 100% of cumulative outcrossing rate). Estimates of pollen contamination ranged from 43.6% (Gangwon-10) to 56.4% (Gangwon-12) with the average of 48.9%. On the basis of pooled cpSSR haplotype of each seed, maximum number of 21 pollen contributors were verified from the seeds reproduced by Kyungbuk-38. Minimum number of 13 pollen contributors were verified in Gangwon-10. Mean of 16.2 pollen contributors were verified from a total of 5 mother trees. In conclusion, considering pretty high outcrossing rates between clones within a seed orchard, it may be expected that a fairly good genetic potential of the seeds, produced in '77 plot of the seed orchard of Japanese red pines at Anmyeon island, may be guaranteed. Observed results from the analysis of mating system of Japanese red pines in a '77 plot of the seed orchard may also provide useful information for the establishment and management of the seed orchard of the progressive generation.

• Journal of Acupuncture Research
• /
• v.31 no.1
• /
• pp.61-73
• /
• 2014

Objectives : The purpose of this study is to estimate the test-retest reliability and the intratest repeatability in measuring the lumbar range of motion of healthy volunteers with wireless microelectromechanical system inertial measurement unit(MEMS-IMU) system and to discuss the feasibility of this system in the clinical setting to evaluate the lumbar spine movement. Methods : 19 healthy male volunteers were participated, who got under 21 points at oswestry disability index(ODI) were adopted. Their lumbar motion were measured with IMU twice in consecutive an hour for the test-retest reliability study. Intratest repeatability was calculated in the two tests separately. The calculated intraclass correlation coefficients(ICC) were discussed and compared with the those of the previous studies. Results : Lumbar range of motion of flexion $41.45^{\circ}$, extension $16.34^{\circ}$, right lateral bending $16.41^{\circ}$ left lateral bending $13.63^{\circ}$ right rotation $-2.47^{\circ}$, left rotation $-0.61^{\circ}$. ICCs were 0.96~1.00(intratest repeatability) and 0.61~0.92(test-retest reliability). Conclusion : This study shows that MEMS-IMU system demonstrates a high test-retest reliability and intratest repeatability by calculated intraclass correlation coefficients. The results of this study represents that wireless inertial sensor measurement system has portable and economical efficiency. By MEMS-IMU system, we can measures lumbar range of motion and analyze lumbar motion effectively.

• Korean Journal of Veterinary Research
• /
• v.45 no.1
• /
• pp.85-91
• /
• 2005

Much of the interest on the chemopreventive properties of herbs and plants has been raised, whereas little is regarding to anti-tumor effect of farming and aquatic products. In the present study, the anti-tumor effect of hot-water extract of a seaweed, BLC (Sargassum fulvellum) and BLC/HEN egg was investigated using MCF-7 cells in vitro and in vivo systems. We found that the BLC extract and BLC/HEN egg inhibited cell proliferation in a dose-dependent manner, which might be mediated through up-regulation of p53. Furthermore, this test compound can directly induce apoptosis in MCF-7 cells, which might be mediated through up-regulation of a pro-apoptotic Bax protein and down-regulation of a anti-apoptotic Bcl-2 protein, not by immune system. Nude mice bearing established breast tumors (with exogenous estradiol) were treated with BLC extract and BLC/HEN egg. Treatment BLC extract and BLC/HEN egg caused a 42% and 71% inhibition of tumor growth, respectively. Both agents caused a significant inhibition of volume and weight growth of estrogen independent human breast tumors established from MCF-7 cells. Our results suggested that BLC extract and BLC/HEN egg have the efficacious effect of human breast cancer not only in vitro but also in vivo.

• Journal of Korean Tunnelling and Underground Space Association
• /
• v.19 no.6
• /
• pp.959-972
• /
• 2017

In evaluation of blast-induced vibration, peak particle velocity (PPV) is generally calculated by using attenuation relation curve. Calculated velocity is compared with the value in legal requirements or the standards to determine the stability. Attenuation relation curve varies depending on frequency of test blasting, geological structure of the site and blasting condition, so it is difficult to predict accurately using such an equation. Since PPV is response value from the ground, direct evaluation of the structure is impractical. Because of such a limit, engineers tend to use the commercial numerical analysis program in evaluating the stability of the structure more accurately. However, when simulate the explosion process using existing numerical analysis program, it's never easy to accurately simulate the complex conditions (fracture, crushing, cracks and plastic deformation) around blasting hole. For simulating such a process, the range for modelling will be limited due to the maximum node count and it requires extended calculation time as well. Thus, this study is intended to simulate the elastic energy after fractured zone only, instead of simulating the complex conditions of the rock that results from the blast, and the analysis of response characteristics of the velocity depending on shape and size of the fractured zone was conducted. As a result, difference in velocity and attenuation character were calculated depending on fractured zone around the blast source appeared. Propagation of vibration tended to spread spherically as it is distanced farther from the blast source.

• Korean Journal of Social Welfare
• /
• v.56 no.3
• /
• pp.61-87
• /
• 2004

In Korea, since the systematic efforts to help unmarried mothers are rather limited, the unmarried mother's social relationship is expected to play a critical role in the adjustment of unmarried mothers after the birth of a baby. The purpose of this study is to identify the effect of social relationship on unmarried mother's self-esteem. Especially, this study focused a negative aspect of social relationship, social conflict, which has been ignored by the previous researches. For this study, survey method was employed. The respondents of the questionnaire were 205 unmarried mothers staying at six out of eight institutions for unmarried mothers available in Korea. The multiple regression and other descriptive statistical methods were conducted and the results of analysis are as follows. First, the higher the level of perceived social support was, the higher the level of self-esteem of unmarried mothers was. Second, the higher the level of perceived social conflict was, the lower the level of self-esteem of unmarried mothers was. Third, even though the level of perceived social conflict was much lower than the perceived social support, the effect of social conflict on unmarried mothers' self-esteem was as powerful as the social support. This means that the effect of social conflict also can be very important. These findings suggest a new direction for intervention for unmarried mothers. Until now, the major target of intervention in social relationship was to strengthen existing social support or establish new social support system. Now, in addition to augmenting social support, we should pay attention to removal of social conflict, since it has significantly powerful effect on the adjustment of unmarried mothers.

• Journal of The Korean Society of Grassland and Forage Science
• /
• v.40 no.2
• /
• pp.117-122
• /
• 2020

This study was conducted to figure out the productivity of Italian ryegrass(IRG) and leaching water characteristics based on horse manure compost level in Jeju. This study was conducted for about six months. Six treatments were established : non-fertilizer(NF), chemical fertilizer 100%(CF), horse manure compost 50% and chemical fertilizer 50% combination(Combination), horse manure compost with 50% of nitrogen (50%), 100% of nitrogen(100%), 150% of nitrogen(150%). The highest amount of dry matter yield of IRG was revealed in CF(11,965±564 kg/ha), and both 150% and Combination were second(p<0.05). Nitrate leaching tended to increase until the third analysis and then decreased. There were not significantly differences among mean nitrate concentrations. The findings of the study suggest that horse manure compost with 50% of nitrogen be applied for IRG as basal fertilization and then 50% of chemical fertilizer be applied as top fertilization.

• The Journal of Korean Institute of Electromagnetic Engineering and Science
• /
• v.24 no.7
• /
• pp.710-721
• /
• 2013

The noise prediction of motor driving system is one of the most important parts in EV/HEV, as the number of power electronic devices increases. This paper describes the mechanism of noise making process and proposes a simulation model of motor driving system for the prediction of the conducted noise. Theoretical calculations and model based simulations were carried out. DOD-dependent-battery parameters were extracted by AC analysis, and an inverter model including dynamic diode was used. Furthermore, 2-D EM tool was used for the motor modeling and was combined with the circuit models of battery and inverter. The simulated voltages, currents and spectrums in the motor driving system showed qualitatively meaningful results, suggesting the validness of the suggested modeling methods.

• Journal of the Korean Ceramic Society
• /
• v.39 no.4
• /
• pp.327-335
• /
• 2002

Experiments were conducted to investigate the synthesis characteristics of Precipitated Calcium Carbonate(for short PCC) in Ca(OH)$_2-CO_2-H_2O$ system by the continuous drop method of Ca(OH)$_2$ slurry into the solution containing $CO_2$(aq). When the flow rate of $CO_2$(g) increases and the concentration of Ca(OH)$_2$ slurry become low, the absorption rate of $CO_2$(g) become faster than the dissolution rate of Ca(OH)$_2$. Consequently, the growth of the calcite crystal plane is facilitated resulting in synthesis of $1.0{\mu}m$ of rhombohedral calcite. On the other hand, when the flow rate of $CO_2$(g) decreases and the concentration of Ca(OH)$_2-CO_2-H_2O$ slurry become high, new nuclei is created along with the crystal growth resulting in synthesis of $0.1{\mu}m$ of prismatic calcite. Maintaining 1.0wt% of Ca(OH)$_2-CO_2-H_2O$ slurry, 120 drops/min of drop rate and $25^{circ}C$ of temperature, the shape of PCC shows colloidal and spherical agglomerate at 100 mL/min of the flow rate of $CO_2$(g); the mixture of rhombohedral and plate-shaped calcite, at 200∼500 mL/min. Therefore, as the flow rate of $CO_2$(g) increases, the shape of PCC changes from colloidal and rhombohedral calcite to plate-shaped calcite. Maintaining 500 mL/min of the flow rate of $CO_2$(g), 120 drops/min of the drop rate of Ca(OH)$_2$ slurry, and $25^{circ}C$ of temperature, the shape of PCC shows the plate-shaped calcite at 1.0∼3.0 wt% of Ca(OH)$_2$ slurry; the hexagonal plate-shape calcite of the thickness of $0.1{\mu}m$ and the width of $1.0{\mu}m$, at 4.0 wt%.

• The Korean Journal of Physiology and Pharmacology
• /
• v.19 no.4
• /
• pp.365-372
• /
• 2015

Aripiprazole (ARI) is a commonly prescribed medication used to treat schizophrenia and bipolar disorder. To date, there have been no studies regarding the molecular pathological and immunotoxicological profiling of aripiprazole. Thus, in the present study, we prepared two different formulas of aripiprazole [Free base crystal of aripiprazole (ARPGCB) and cocrystal of aripiprazole (GCB3004)], and explored their effects on the patterns of survival and apoptosis-regulatory proteins under acute toxicity and cytotoxicity test conditions. Furthermore, we also evaluated the modulatory activity of the different formulations on the immunological responses in macrophages primed by various stimulators such as lipopolysaccharide (LPS), pam3CSK, and poly(I:C) via toll-like receptor 4 (TLR4), TLR2, and TLR3 pathways, respectively. In liver, both ARPGCB and GCB3004 produced similar toxicity profiles. In particular, these two formulas exhibited similar phospho-protein profiling of p65/nuclear factor $(NF)-{\kappa}B$, c-Jun/activator protein (AP)-1, ERK, JNK, p38, caspase 3, and bcl-2 in brain. In contrast, the patterns of these phospho-proteins were variable in other tissues. Moreover, these two formulas did not exhibit any cytotoxicity in C6 glioma cells. Finally, the two formulations at available in vivo concentrations did not block nitric oxide (NO) production from activated macrophage-like RAW264.7 cells stimulated with LPS, pam3CSK, or poly(I:C), nor did they alter the morphological changes of the activated macrophages. Taken together, our present work, as a comparative study of two different formulas of aripiprazole, suggests that these two formulas can be used to achieve similar functional activation of brain proteins related to cell survival and apoptosis and immunotoxicological activities of macrophages.

• Nutrition Research and Practice
• /
• v.12 no.2
• /
• pp.129-134
• /
• 2018

BACKGROUND/OBJECTIVES: Although several recent studies have reported the anti-cancer effects of extracts or components of Citrus unshiu peel, which has been used for various purposes in traditional medicine, the molecular mechanisms for their effects remain unclear. In the present study, the anti-cancer activity of a water-soluble extract of C. unshiu peel (WECU) in MDA-MB-231 human breast carcinoma cells at the level of apoptosis induction was investigated. MATERIALS/METHODS: Cytotoxicity was evaluated using the MTT assay. Apoptosis was detected using DAPI staining and flow cytometry analyses. Mitochondrial membrane potential, reactive oxygen species (ROS) assay, caspase activity and Western blotting were used to confirm the basis of apoptosis. RESULTS: The results indicated that WECU-induced apoptosis was related to the activation of caspase-8, and -9, representative initiator caspases of extrinsic and intrinsic apoptosis pathways, respectively, and caspase-3 accompanied by proteolytic degradation of poly(ADP-ribose) polymerase and down-regulation of the inhibitors of apoptosis protein family members. WECU also increased the pro-apoptotic BAX to anti-apoptotic BCL-2 ratio, loss of mitochondrial membrane potential and cytochrome c release from mitochondria to cytoplasm. Furthermore, WECU provoked the generation of ROS, but the reduction of cell viability and induction of apoptosis by WECU were prevented when ROS production was blocked by antioxidant N-acetyl cysteine. CONCLUSIONS: These results suggest that WECU suppressed proliferation of MDA-MB-231 cells by activating extrinsic and intrinsic apoptosis pathways in a ROS-dependent manner.