• Proceedings of the Korean Nutrition Society Conference
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• 2003.05a
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• pp.296.1-296.1
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• 2003

• Korean Journal of Medicinal Crop Science
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• v.16 no.3
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• pp.139-143
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• 2008

The antioxidant activities of Agrimony (Agrimonia pilosa L.) and Chinese lizardtail (Saururus chinensis Baill) according to extraction methods were measured. SOD-like activity showed greater antioxidant effects with ethanol solvent than those with water. Ethanol extracts of Agrimony leaves showed the highest SOD-like activity of 94.4%. SOD-like activity differed according to the extraction solvents. The contents of polyphenolic compounds were higher in water extracts than those in ethanol extracts. The contents were 161.4 mg for Agrimony roots, 100.2 mg for Agrimony leaves, and 79.1 mg for Agrimony stalks in order. EDA in Agrimony leaves that were highest among medicinal plants were 83.4% in the water extract and 81.7% in the ethanol extract. The anticancer effects of the extracts by water and ethanol from Agrimony and Chinese lizardtail were experimented. The growth of stomach cancer cells, SNU-719 was inhibited 94.5% by the hexane fractions of Agrimony and also the growth of liver cancer cells, Hep3B was inhibited 83.2% by the hexane fractions of Agrimony, while the growth of normal cell, DC2.4 was not affected.

• Korean Journal of Medicinal Crop Science
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• v.8 no.2
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• pp.129-133
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• 2000

The experiment was conducted to increase germination of Agrimonia pilosa seeds, one of the natural medicinal plants, by various treatments, and also the effects of seed treatments on the growth of plants after sowing in field. The optimum temperature for germination of A. pilosa seeds was $20^{\circ}C$ after 20 days stratification duration at $4^{\circ}$, the germination rate than highest, 86% at that temperature. The stratification increased germination rate in all the temperatures treated, shortened $26{\sim}32$ days of germination period and promoted more 20% germination rate than the other treatments. Flowing water, low temperature, $GA_3$ and NaOCl were not effective for germination and high temperature reduced germination rate. At the early growth stage, the plant height was taller $3{\sim}4\;cm$, one or more leaves were increased by the stratification treatments, and also stratification increased the number of leaves per plants 10 or more leaves compare with other treatments at harvesting stages. By stratification, the flowering date was 5 days earlier, June 18th than other treatments due to earlier sprouting.

• Journal of the Korean Society of Food Science and Nutrition
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• v.41 no.2
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• pp.161-167
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• 2012

To evaluate the anti-obesity effect of Agrimonia pilosa L., this study investigated that ethyl acetate extract from A. pilosa L. (EAAP) suppresses lipid accumulation and inhibits expression of adipogenic marker genes, such as peroxisome proliferator activated receptor ${\gamma}$ (PPAR${\gamma}$), CCAAT-enhancer-binding protein ${\alpha}$ (C/EBP${\alpha}$), glucose transporter 4 (GLUT4), and adiponectin in 3T3-L1 preadipocytes. We demonstrated that EAAP inhibited adipocyte differentiation and expression of PPAR${\gamma}$ and C/EBP${\alpha}$ mRNA levels in a dose-dependent manner. In addition, EAAP reduced the PPAR${\gamma}$ transcriptional activity stimulated by rosiglitazone in HEK 293T cells and decreased the expression of GLUT4 and adiponectin in 3T3-L1 cells. These results suggest that EAAP inhibits preadipocyte differentiation and adipogenesis by blocking of PPAR${\gamma}$ and C/EBP${\alpha}$ gene expression in 3T3-L1 cells.

• Research in Plant Disease
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• v.14 no.3
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• pp.201-204
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• 2008

We've been involved in collection and domestication of Agrimonia pilosa from 2005 to 2007. We observed agrimony powdery mildew caused by Sphaerotheca aphanis. The powdery mildew developed twice a year, in May to June and mid August to September on whole plants of A. pilosa plants, that is, leaves, stem and floral part. We sprayed the Cooking Oil & Yolk mixture (COY) to control the agrimony powdery mildew. When COY was single-sprayed to control agrimony powdery mildew, its control value was 26.9%. However when it was double-sprayed and triple-sprayed at seven days interval, its control value increased up to 80.8 and 82.7%, respectively. Upon the first spray, symptoms of powdery mildew disappeared within three days and suppressive effect of COY was persisted for only seven days. Thereafter, powdery mildew was relapsed to develop. However, on double to triple sprays, powdery mildew was suppressed more than 28 days after final treatment. Therefore, it is concluded that double spray of COY at seven days interval can control effectively agrimony powdery mildew.

• Journal of Food Hygiene and Safety
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• v.23 no.2
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• pp.149-156
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• 2008

This study was carried out to investigate the anti oxidative and antitumor activities of medicinal plants for the purpose of developing a functional food. The methanol extracts of Agrimonia pilosa was fractionated with five solvents and examined anti oxidative activities and enzyme inhibitory activities in addition to growth inhibitory activity of human cancer cell. The contents of total phenol compounds in EtOAc and BuOH fraction were 39.89% and 39.56%, respectively. Strong electron donating abilities(>90%) were shown in these fractions and its abilities were 92.90% ($500\;{\mu}g/ml$), 94.47% ($1000\;{\mu}g/ml$) in EtOAc fraction and 93.77% ($500\;{\mu}g/ml$), 92.90% ($1000\;{\mu}g/ml$) in BuOH fraction, respectively. These fractions exhibited more than 50% nitrite scavenging ability and potent inhibition activities to XOase activity (93.06%, 91.73%) at concentration of $1000\;{\mu}g/ml$. In antitumor activity test, hexane fraction showed the strongest growth inhibition activity against HT-29, SNU-1 and HeLa cells. Inhibition levels were 51.50, 90.09% in HT-29, 88.19, 95.11 % in SNU-1 and 42.66, 96.40% in HeLa at the concentration of 50, $100\;{\mu}g/ml$, respectively.

• Journal of Applied Biological Chemistry
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• v.55 no.1
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• pp.35-39
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• 2012

The purpose of this study was to research about the ingredients for anti-oxidation and anti-wrinkle effects of the solvent fractions from Agrimonia pilosa L. hot water (AW) and 70% ethanol (AE) extracts. The electron donating ability of the solvent fractions from AW and AE extracts showed 84.9, 92.5% in ethyl acetate layer of AW and AE at 1,000 ppm. The superoxide dismutase like activity of solvent fractions from AW and AE extracts showed 61.8% in ethyl acetate layer of AW extracts and 58.0% in buthanol layer of AE extracts at 1,000 ppm. For anti-wrinkle effect, elastase inhibition effect of the solvent fractions from AW and AE extracts showed 55.2, 70.1% in ethyl acetate layer of AW and AE extracts at 1,000 ppm. And collagenase inhibition effect of the solvent fractions from AW and AE extracts showed highest inhibition effect as 90.6% in ethyl acetate layer of AW extract and 88.6% in $n$-butyl alcohol layer of AE extract at 1,000 ppm. All these findings suggested that solvent fractions from AW and AE extracts has an anti-oxidation and anti-wrinkle effects.

• Korean Journal of Plant Resources
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• v.15 no.3
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• pp.243-249
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• 2002

This experiment was conducted to find out the effects of livestock excrements application on the growth and yield of leaf, stem and seed in Agrimonia pilosa Ledeb. the results were summarized as follow. In the soil after experiment, P2O5 and K contents were high in order of cattle droping 〈 pig droping 〈 chicken droping treatment. The plant height was increased cattle dropping〈 pig dropping〈 chicken dropping treatment plot. Number of stems was inclined to increase in the plot of 2,000 kg/10a application of chicken dropping and cattle dropping. Number of leaves was also increased 21.8∼51.6% by application 3,000 kg/10a of chicken and cattle dropping, and application 2,000 kg/10a of pig dropping, respectively. Dry weight of aerial part was high in order of 1,000〈 3,000〈 2,000 kg/10a〉control, on occasion 2,000 kg/10a treated, that was highest in the application of chicken dropping. Yield of seeds was also increased to 61％ and 86% at the 2,000 kg/10a plot of pig and chicken dropping, respectively. While yield of seeds was increased 50％ at the 3,000 kg/10a application of cattle dropping. Flowering ratio was 49.1％ in control, 55.6, 58.9 and 68.3% in cattle, pig and chicken dropping with 2,000 kg/10a, respectively, on June 22. Flowering ratio was the highest in the plot of chicken dropping with 2,000 kg/10a.

• Journal of Society of Preventive Korean Medicine
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• v.13 no.2
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• pp.51-64
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• 2009

Objective : This research was aimed to investigate the anti-tumor effect, safety, mechanism and metabolizing enzyme of Agrimonia pilosa LEDEB(APL) in female C57B/L mouse. Methods : At first, to evaluate the anti-tumor activity of APL, we divided into four groups, normal, control, APL100(100mg/kg), APL150(150mg/kg). LLC obtained American Type Culture Collection was used. LLC had been inoculated to induce tumor. To measure the anti-tumor effect of APL, we calibrate tumor size and weight. To study for mechanism of anti-tumor in APL, we used western blotting and to know metabolizing enzyme in APL we used to real-time PCR. Results : APL100, APL150 inhibited tumor growth after medicine injected. APL did not only induced caspase-dependent apoptosis in LLC-bearing mouse tumor. In APL100, it were decreased 72% in CYP3A11. In APL150, it were decreased 62%, 75% in CYP3A11 and MRP1a respectively. Conclusion : These results suggests that APL has some anti-tumor effects in female C57B/L mouse tumor. APL should be careful use with other drugs related with CYP3A11 or MRP1a.

• Journal of Food Hygiene and Safety
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• v.11 no.3
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• pp.209-214
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• 1996

Irradiated and non-irradiated Korean medicinal herbs were extracted by water and 70% ethanol. Antimicrobial activity of these extracts were invesigated against selected food hygiene microoganisms. The ethanol extracts of the non-irradiated Agrimonia pilosa ledebour japonica Nakai, Curcuma longa Linne and Angelica gigas Nakai were completely inhibited on four species of bacteria, such as Vibrio parahaemolyticus, Clostridium perfringenes, Bacillus subtilis and Staphylococcus aureus. Also, above four strains did not have antibacterial activity in the water-ethanol mixtures. Futhermore, the ehtanol extracts of the non-irradiated Agimonia pilosa ledebour Japonica Nakai, Curcuma Iedoaria Roscoe, Curcuma longa Linne and Scutellaria baikalensis George were shown inhibitory effects against Aspergillus flavus and Pen icillium islandicum. And the water extract of Scutellaria baikalensis George was the same effect to these molds. Essentially the same results were observed when samples irradiated at a dose of 10 kGy.