• Title/Summary/Keyword: Agriculture and Forestry

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Seed Storage Method and Seed Germination Characteristics of Synurus deltoides(Ait.) Nakai (수리취의 종자저장 방법과 발아특성)

  • Noh, H.S.;Kwon, H.K.;Sung, J.W.;Shim, Y.J.;Lee, K.C.
    • Journal of Practical Agriculture & Fisheries Research
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    • v.24 no.1
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    • pp.29-35
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    • 2022
  • This study was conducted to investigate the seed storage method and seed germination characteristics of Synurus deltoides (Ait.) Nakai, a kind of wild vegetables. Several parameters affecting seed germination, such as germination temperature, chilling duration, imbibition duration, and storage method were evaluated. The seed germination of Synurus deltoides showed the highest germination rate (GR), promptness index (PI), germination performance index (GPI), mean daily germination (MDG) at 25℃. This implies that the optimum germination temperature was 25℃. And also, Seeds stored at -4℃, after 150 days showed relatively high germination rate about 98%. Therefore, The optimum germination temperature of Synurus deltoides was 25℃ and to increase the germination rate over 98% of Synurus deltoides, cold-wet storage 20 days treatments at 4℃ were effective in the improvement of seed germination.

Establishment of a diagnostic method for porcine proliferative enteropathy using polymerase chain reaction (중합효소연쇄반응을 이용한 돼지 증식성 장염 진단기법 확립)

  • Lym, Suk-kyung;Lee, Hee-soo;Woo, Sung-ryong;Yoon, Soon-seek;Moon, Oun-kyong;Lee, Yoo-young;Koh, Hong-bum
    • Korean Journal of Veterinary Research
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    • v.39 no.1
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    • pp.118-125
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    • 1999
  • Porcine Proliferative Enteropathy(PPE) is an infectious enteric disease and a major cause of economic loss in swine industry due to weight loss, poor growth and sudden death in growing and finishing pigs at 6 to 20 weeks of age. PPE has been diagnosed by clinical signs, syndrom and lesions in the intestine in Korea. However, the diagnostic method had several problems in the detection of infected or carrier pigs. Therefore, in this study, we established the polymerase chain reaction(PCR) which was a fast, specific and sensitive method for identification of Lawsonia intracellularis (L intracellularis). We designed and synthesized primer on the 16S rDNA and p78 gene encoding L intracellularis. Specificity of the method was confirmed by comparison of the PCR results using other enteric bacteria and the study has shown that PCR method was sensitive to detect 1ng of genomic DNA as a template. Identity of the PCR products was confirmed by comparison of pattern of restriction endonuclease analysis with restriction enzyme Hae III and Pst I. Also, the PCR method was applicable to the naturally affected pigs with PPE. Based on the results from this study, the PCR method could be used as a fast and specific diagnostic tool for PPE.

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Efficient (3R)-Acetoin Production from meso-2,3-Butanediol Using a New Whole-Cell Biocatalyst with Co-Expression of meso-2,3-Butanediol Dehydrogenase, NADH Oxidase, and Vitreoscilla Hemoglobin

  • Guo, Zewang;Zhao, Xihua;He, Yuanzhi;Yang, Tianxing;Gao, Huifang;Li, Ganxin;Chen, Feixue;Sun, Meijing;Lee, Jung-Kul;Zhang, Liaoyuan
    • Journal of Microbiology and Biotechnology
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    • v.27 no.1
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    • pp.92-100
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    • 2017
  • Acetoin (AC) is a volatile platform compound with various potential industrial applications. AC contains two stereoisomeric forms: (3S)-AC and (3R)-AC. Optically pure AC is an important potential intermediate and widely used as a precursor to synthesize novel optically active materials. In this study, chiral (3R)-AC production from meso-2,3-butanediol (meso-2,3-BD) was obtained using recombinant Escherichia coli cells co-expressing meso-2,3-butanediol dehydrogenase (meso-2,3-BDH), NADH oxidase (NOX), and hemoglobin protein (VHB) from Serratia sp. T241, Lactobacillus brevis, and Vitreoscilla, respectively. The new biocatalyst of E. coli/pET-mbdh-nox-vgb was developed and the bioconversion conditions were optimized. Under the optimal conditions, 86.74 g/l of (3R)-AC with the productivity of 3.61 g/l/h and the stereoisomeric purity of 97.89% was achieved from 93.73 g/l meso-2,3-BD using the whole-cell biocatalyst. The yield and productivity were new records for (3R)-AC production. The results exhibit the industrial potential for (3R)-AC production via whole-cell biocatalysis.

Precision Forestry Using Remote Sensing Techniques: Opportunities and Limitations of Remote Sensing Application in Forestry (원격탐사 기술의 국내 정밀 임업 가능성 검토: 임업분야의 원격탐사 적용사례 분석을 중심으로)

  • Woo, Heesung;Cho, Seungwan;Jung, Geonhwi;Park, Joowon
    • Korean Journal of Remote Sensing
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    • v.35 no.6_2
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    • pp.1067-1082
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    • 2019
  • This review paper presents a review of evidence on systems and technologies for recent remote sensing techniques which were applied into forest and forest related sectors. The paper reviewed remote sensing techniques that will have, or already having, a substantial impact on improving data quality of forest inventory and forest management and planning. The aim of this review is to identify, categorize and discuss Korean and international sources published primarily in the last decades. The focus on remote sensing and ICT technologies examines issues related to their opportunities, limitation, use and impact on the forestry. More specifically, this literature review has focused on laser scanning, satellite imagery, and Unmanned aerial vehicles (UAV) utilization in forest management and inventory analysis.

Regeneration and leaf traits variation of Rhododendron campanulatum along elevation gradient in western Nepal Himalaya

  • Dipesh Karki;Bijay Pandeya;Rachana Bhandari;Dikshya Basnet;Balkrishna Ghimire;Shreehari Bhattarai;Bharat Babu Shrestha
    • Journal of Ecology and Environment
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    • v.48 no.2
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    • pp.152-162
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    • 2024
  • Background: Plant species of the alpine treeline ecotone are highly sensitive to climate change and may adjust their population dynamics, and functional traits in response to changing climate. This study examined regeneration patterns and leaf traits variations in an important treeline ecotone element Rhododendron campanulatum along the elevation gradient in western Nepal to assess its potential adaptive responses to climate change. The distribution range of R. campanulatum (3,400-3,800 m above sea level [a.s.l.]) was divided into five horizontal bands, each with a 100 m elevational range. Eight plots (10 m × 10 m) were sampled in each band, resulting into a total of 40 plots. In each plot, all R. campanulatum individuals and co-occurring tree species were counted. From each elevation, R. campanulatum leaf samples were collected to determine leaf dimensions, leaf density, specific leaf area (SLA), and stomatal density (SD). Results: The density-diameter curve indicated that R. campanulatum was regenerating well, with enhanced regeneration at higher elevation (3,800 m a.s.l.) than at lower. Tree canopy cover appeared to be the major determinant of R. campanulatum regeneration, as indicated by a higher number of seedlings in treeless stands. With increasing elevation, the leaf length, width, SLA, and stomata length decreased but leaf thickness and SD increased. Conclusions: Overall, a higher regeneration and lower SLA with the high SD in the leaves at the upper limit of the species distribution suggested that R. campanulatum is well adapted at its upper distribution range with the possibility of upslope range shift as temperature increases.

Data Modeling of FRIS Standard Information (FRIS 표준정보 및 데이터 모델링)

  • Yang, Jin-Hyuk;Kim, Sung-Il;Kim, Hye-Jin;Yoon, Ki-Ho
    • Proceedings of the Korean Information Science Society Conference
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    • 2012.06c
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    • pp.137-138
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    • 2012
  • FRIS 표준정보는 농림수산식품분야 R&D정보의 체계적인 수집, 연계 및 공동활용을 위한 정보이다. 본 논문에서는 FRIS 표준정보와 그 데이터 모델링 결과를 제시함으로써, 농림수산식품분야의 R&D관리기관 업무 담당자들과 연구수행자들에게 FRIS에서 수집, 공유, 활용되는 R&D정보에 대한 이해와 활용을 증진 시키고자 한다.

A Study on Types and Characteristics of Experience and Tourism Business Plan of Agriculture, Forestry and Fishery Sector for Basic Settlement Area Development Plan (기초생활권 발전계획 농림수산부문의 체험 및 관광사업 유형 및 특성에 관한 연구)

  • Lee, Kyung-Jin;Kim, Nam-Hyun
    • Journal of Agricultural Extension & Community Development
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    • v.18 no.4
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    • pp.825-860
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    • 2011
  • For the first time basic settlement area development plan was established in 2010, in order to promote cooperative coexisting development among regions, as time that regional competitiveness determines national competitiveness started. Basic settlement area development plan is composed of 7 sectors and 24 general subsidy projects. It was judged that experience and tourism business as the center is required to be analyzed, which may have effect on activation of farm village, fishing village and mountain village, and inflow of city people, in agriculture, forestry and fishery sector in order that added value of agriculture and fishery is increased, and tangible and intangible resources are applied, and pure agriculture, forestry and fishery is developed. And currently farm village, mountain village and fishing village of our country faces desperate crisis situation that they cannot help groping for substitutive to create new revenue model, and agriculture and fishery of primary industry has limit of increasing income of farmer and fisherman. Agriculture, forestry and fishery experience and tourism business was classified by types, and then standard of 12 categories and 48 sections was prepared, for searching method to supplement and develop it. Trend of experience and tourism business was understood, and problem was found, and then it may be used as indicator material to carry out creative and differentiated business plan establishment and business operation, and may be opportunity to reject overlapped business among regions, and to promote balanced regional development.

Development of PCR-based DNA markers for identification and detection of Trichoderma species associated with the green mold disease of oyster mushroom (느타리버섯 푸른곰팡이병에 관여하는 Trichoderma 속균의 동정 및 검출을 위한 PCR 기반 DNA 마커 개발)

  • Park, Myung Soo;Seo, Geon Sik;Ryu, Jae San;Kim, Min Kyung;Lee, Yong Kuk
    • Journal of Practical Agriculture & Fisheries Research
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    • v.24 no.3
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    • pp.5-14
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    • 2022
  • Trichoderma is known as pathogen caused serious green mold disease on commercial production. T. pleuroti and T. pleuroticola were common species in various mushroom media. Many strains of T. pleuroti, known as aggressive species causing major economic losses in Korea, showed benomyl resistance. Accurate identification and detection of Trichoderma species associated with oyster mushrooms is very important for disease control. We developed species-specific primers for T. pleuroticola, T. pleuroti, T. harzianum, and T. atroviride based on species-specific fragments isolated from amplified fragment length polymorphism analysis. PCR products corresponding to the predicted fragment of 500bp, 230bp, 180bp, and 410bp were amplified from T. pleuroticola, T. pleuroti, T. harzianum, and T. atroviride, respectively. Multiplex PCR assay using species-specific primers quickly and accurately identified and detected T. pleuroti from mushroom media in which various species co-exist. Our results can be useful for the effective control of mushroom disease.

Diversity and distribution of invasive alien plant species along elevation gradient in Makawanpur district, central Nepal

  • Dipesh Karki;Bijay Pandeya;Balkrishna Ghimire
    • Journal of Ecology and Environment
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    • v.47 no.3
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    • pp.75-84
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    • 2023
  • Background: Knowledge of the spatial trends of plant invasions in different habitats is essential for a better understanding of the process of these invasions. We examined the variation in invasive alien plant species (IAS) richness and composition at two spatial scales defined by elevation and habitat types (roadside, forest, and cultivated lands) in the Makawanpur district of Nepal. Following an elevation gradient ranging from 500 to 2,400 m asl along a mountain road, plant species cover was recorded within sample plots of size 10 m × 5 m. Systematic random sampling was adopted in every 100 m elevation intervals on three habitat types. Results: Altogether 18 invasive alien plants belonging to eight families were recorded within 60 plots, of which 14 species (representing 80%) were from tropical North and South America. The most common plants by their frequency were Ageratina adenophora, Chromolaena odorata, Bidens pilosa, Lantana camara, and Parthenium hysterophorus. We found a significant relationship between species composition and elevation in the study area. Low-elevation regions had a higher number of alien species as compared to high-elevation regions within different habitat types. Conclusions: The species richness and density of IAS were higher in the road site followed by the cultivated land and forest sites. This pattern occurred throughout the elevation range and habitats. IAS were found mostly in the open land with high sunlight availability. Information from such scientific assessment of invasive alien plants will assist in developing appropriate management plans in the Makawanpur district.

The Carbon Sequestration Potential of Forestry Sector: Bangladesh Context

  • Sohel, Md. Shawkat Islam;Rana, Md. Parvez;Alam, Mahbubul;Akhter, Sayma;Alamgir, Mohammed
    • Journal of Forest and Environmental Science
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    • v.25 no.3
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    • pp.157-165
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    • 2009
  • Forests potentially contribute to global climate change through their influence on the global carbon (C) cycle. The Kyoto Protocol provides for the involvement of developing countries in an atmospheric greenhouse gas reduction regime under its Clean Development Mechanism (CDM). Carbon credits are gained from reforestation and afforestation activities in developing countries. Bangladesh, a densely populated tropical country in South Asia, has a huge degraded forestland, which can be reforested by CDM projects. To realize the potential of the forestry sector in developing countries like Bangladesh for full-scale emission mitigation, the carbon sequestration potential should be integrated with the carbon trading system under the CDM of the Kyoto Protocol. This paper discusses the prospects of carbon trading in Bangladesh, in relation to the CDM, in the context of global warming.

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