• Journal of Biosystems Engineering
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• 제43권4호
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• pp.394-400
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• 2018

Purpose: This research evaluated five types of nanoparticles to develop a surface-enhanced Raman spectroscopy (SERS) method for the rapid detection of two Bacillus species (Bacillus cereus and Bacillus thuringiensis) that are commonly found on fresh produce, which can cause food poisoning. Methods: Bacterial concentrations were adjusted to a constant turbidity, and a total of $30{\mu}L$ of each Bacillus cell suspension was prepared for each nanoparticle. A point-scan Raman system with laser light source of wavelength 785 nm was used to obtain SERS data. Results: There was no qualitative difference in the SERS data of B. cereus and B. thuringiensis for any of the five nanoparticles. Three gold nanoparticles, stabilized in either citrate buffer or ethanol, showed subtle differences in Raman intensities of two Bacillus species at $877.7cm^{-1}$. Conclusions: Among the three types of nanoparticles, the gold nanoparticles stabilized in citrate buffer showed the lowest standard deviation, followed by gold nanoparticles stabilized in ethanol. This result supports the potential application of gold nanoparticles for SERS-based detection of B. cereus and B. thuringiensis.

• 농촌지도와개발
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• 제29권3호
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• pp.131-142
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• 2022

This study aims to develop a curriculum for nurturing experts who perform agricultural safety and health management tasks. This study was conducted in three stages. First, job definitions and job models of agricultural safety and health managers were derived through job analysis using the DACUM technique. Second, job demand analysis was conducted by conducting a survey on the difficulty, importance, and frequency of each task. Third, IPA analysis was performed as the first priority tasks of job demand analysis to present the courses and subjects of the training course for safety and health managers in agricultural work. The job of the agricultural safety and health manager was defined as "to conduct guidance and advice on safety and health education, risk factors, and evaluation and management of harmful factors to protect farmers' professional health and safety." The job model consisted of 4 tasks, 31 core tasks, and 67 detailed tasks. As a result of job demand analysis, there were 39 tasks in the 1st priority, 22 in the 2nd priority, and 6 in the 3rd priority. As a result of the IPA analysis, there were 13 'capacity development focused areas', 4 'capacity development effort areas', 11 'low priority areas', and 3 'continuous maintenance areas'.

• 한국작물학회:학술대회논문집
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• 한국작물학회 2017년도 9th Asian Crop Science Association conference
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• pp.158-158
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• 2017

The purpose of this study was to identify the candidate genes involved in selenium content in brown rice. Rice (Oryza sativa L.) was important crop including diverse functional substance such as carbohydrate, protein, lysine and tocopherol, mineral. Especially, selenium as nutritionally important minerals, it was known to activate the immune system, antioxidant effect and inhibition of carcinogenesis. Also recommended daily requirements of the United States and the United Kingdom were 55 to 90 ug for selenium. Therefore, selenium content in brown rice of core-set were analyzed by using ICP-MS (Inductively Coupled Plasma Mass Spectrometer) and GWAS (Genome Wide Association Study) was conducted to search for candidate genes in this study. The new natural variants identified through haplotyping analysis would be useful to develop new rice varieties with improved storage ability of the valuable mineral through the future molecular breeding.

• 한국토양비료학회지
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• 제50권6호
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• pp.663-673
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• 2017

Heavy metal(loid) contamination of rice is the main issue in abandoned metal mine area with regard to food safety. A field study was conducted in mine area to see if soil amendments treatment including calcium superphosphate, sulfur, steel slag and S-containing fertilizer could reduce As and/or Cd content in rice grain and increase the growth of rice. The As content in brown rice reduced to 60% compared to the control only in $7.0Mg\;ha^{-1}$ of steel slag treatment. Cd reduction in rice was thought to be not the effect of amendments but the result from the difference in growth and development of rice plant and this could be ascribed to low soil Cd availability to rice plant. Compared with control, increased rice yield of cultivar Hwanggeumnuri was 1.3~2.2 and $1.5Mg\;ha^{-1}$ in calcium super phosphate and S-containing fertilizer treatment, respectively and the trend was also observed in cultivar Ungwang. However, steel slag treatment reduced the Ungwang yield by $0.4{\sim}0.9Mg\;ha^{-1}$. Future work will be needed to establish the agricultural measure with which secure the safety and yield of rice simultaneously.

• Journal of Biosystems Engineering
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• 제42권3호
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• pp.170-189
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• 2017

Purpose: This paper presents Raman chemical imaging technology for inspecting food and agricultural products. Methods The paper puts emphasis on introducing and demonstrating Raman imaging techniques for practical uses in food analysis. Results & Conclusions: The main topics include Raman scattering principles, Raman spectroscopy measurement techniques (e.g., backscattering Raman spectroscopy, transmission Raman spectroscopy, and spatially offset Raman spectroscopy), Raman image acquisition methods (i.e., point-scan, line-scan, and area-scan methods), Raman imaging instruments (e.g., excitation sources, wavelength separation devices, detectors, imaging systems, and calibration methods), and Raman image processing and analysis techniques (e.g., fluorescence correction, mixture analysis, target identification, spatial mapping, and quantitative analysis). Raman chemical imaging applications for food safety and quality evaluation are also reviewed.

• 한국농공학회논문집
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• 제65권4호
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• pp.33-43
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• 2023

In this study, it was developed and verified an application method of fuzzy-logic theory to the rating process of agricultural fill dam safety. A fuzzy-logic is very famous logical system when some decision making is made on the status of a lack of information. Three proxies were selected and configured membership functions (MFs) and these MFs were activated in the process of fuzzification procedures. Fuzzified vlaues were passed through the rule-based inference system, then fire strength could classified among cases of the rule-based inference system. To obtain final results, Mandani-type was adapted in the defuzzification process. As the results, it was shown the developed system can give a correct results that was compared with Matlab - fuzzy inference function. More ever it could perform the detailed analysis and improvement on the infrastructure safety rating process using classical diagnosis method.

• Agribusiness and Information Management
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• 제3권1호
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• pp.11-22
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• 2011

This study describes the development of a web-based system that collects all data generated in the research conducted to set pre-harvest residue limits (PHRLs) for agricultural product safety control. These data, including concentrations of pesticide residues, limit of detection, limit of quantitation, recoveries, weather charts, and growth rates, are incorporated into a database, a regression analysis of the data is performed using statistical techniques, and the PHRL for an agricultural product is automatically computed. The development and establishment of this system increased the efficiency and improved the reliability of the research in this area by standardizing the data and maintaining its accuracy without temporal or spatial limitations. The system permits automatic computation of the PHRL and a quick review of the goodness of fit of the regression model. By building and analyzing a database, it also allows data accumulated over the last 10 years to be utilized.

• The Plant Pathology Journal
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• 제38권6호
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• pp.637-645
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• 2022

Fusarium head blight (FHB) is one of the most serious diseases in barley and wheat, as it is usually accompanied by the production of harmful mycotoxins in the grains. To identify FHB-resistant breeding resources, we evaluated 60 elite germplasm accessions of barley (24) and wheat (36) for FHB and mycotoxin accumulation. Assessments were performed in a greenhouse and five heads per accession were inoculated with both Fusarium asiaticum (Fa73, nivalenol producer) and F. graminearum (Fg39, deoxynivalenol producer) strains. While the accessions varied in disease severity and mycotoxin production, four wheat and one barley showed <20% FHB severity repeatedly by both strains. Mycotoxin levels in these accessions ranged up to 3.9 mg/kg. FHB severity was generally higher in barley than in wheat, and Fa73 was more aggressive in both crops than Fg39. Fg39 itself, however, was more aggressive toward wheat and produced more mycotoxin in wheat than in barley. FHB severity by Fa73 and Fg39 were moderately correlated in both crops (r = 0.57/0.60 in barley and 0.42/0.58 in wheat). FHB severity and toxin production were also correlated in both crops, with a stronger correlation for Fa73 (r = 0.42/0.82 in barley, 0.70 in wheat) than for Fg39.

• 농약과학회지
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• 제17권4호
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• pp.473-477
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• 2013

본 연구는 병해충 관리용 유기농업자재 허용물질로 지정된 식물추출물 중 님 추출물과 고삼추출물, 데리스 추출물 원제의 독성 평가를 위해 꿀벌에 대한 급성 접촉 및 섭식독성시험을 수행하였다. 급성접촉독성시험결과 님 추출물 2종과 데리스 추출물 2종은 $LD_{50}$이 100 ${\mu}g/bee$ 이상으로 독성이 매우 낮았고 고삼 추출물은 2종 모두 $LD_{50}$(48h)이 1.7 ${\mu}g/bee$로 매우 강한 독성을 보였다. 급성섭식독성시험결과 님 추출물 2종과 데리스 추출물 2종은 $LD_{50}$이 100 ${\mu}g/bee$ 이상으로 독성이 매우 낮았고 고삼 추출물A는 $LD_{50}$(48h)이 1.7 ${\mu}g/bee$, 고삼 추출물B는 $LD_{50}$(48h)이 0.3 ${\mu}g/bee$으로 모두 매우 강한 독성이 있는 것으로 평가되었다.

• Journal of Microbiology and Biotechnology
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• 제27권7호
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• pp.1276-1280
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• 2017

The mcr-1 gene is a new "superbug" gene discoverd in China in 2016 that makes bacteria highly resistant to the last-resort class of antibiotics. The mcr-1 gene raised serious concern about its possible global dissemination and spread. Here, we report a potential anti-resistant strategy using the CRISPR/Cas9-mediated approach that can efficiently induce mcr-1 gene knockout in Escherichia coli. Our findings suggested that using the CRISPR/Cas9 system to knock out the resistance gene mcr-1 might be a potential anti-resistant strategy. Bovine myeloid antimicrobial peptide-27 could help deliver plasmid pCas::mcr targeting specific DNA sequences of the mcr-1 gene into microbial populations.