Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Generation of Newly Discovered Resistance Gene mcr-1 Knockout in Escherichia coli Using the CRISPR/Cas9 System

  • Sun, Lichang (Key Laboratory of Control Technology and Standard for Agro-product Safety and Quality Ministry of Agriculture, Key Laboratory of Food Quality and Safety of Jiangsu Province-State Key Laboratory Breeding Base, Institute of Food Quality and Safety, Jiangsu Academy of Agricultural Sciences) ;
  • He, Tao (Key Laboratory of Control Technology and Standard for Agro-product Safety and Quality Ministry of Agriculture, Key Laboratory of Food Quality and Safety of Jiangsu Province-State Key Laboratory Breeding Base, Institute of Food Quality and Safety, Jiangsu Academy of Agricultural Sciences) ;
  • Zhang, Lili (Key Laboratory of Control Technology and Standard for Agro-product Safety and Quality Ministry of Agriculture, Key Laboratory of Food Quality and Safety of Jiangsu Province-State Key Laboratory Breeding Base, Institute of Food Quality and Safety, Jiangsu Academy of Agricultural Sciences) ;
  • Pang, Maoda (Key Laboratory of Control Technology and Standard for Agro-product Safety and Quality Ministry of Agriculture, Key Laboratory of Food Quality and Safety of Jiangsu Province-State Key Laboratory Breeding Base, Institute of Food Quality and Safety, Jiangsu Academy of Agricultural Sciences) ;
  • Zhang, Qiaoyan (Zhejiang Province Key Laboratory for Food Safety, Institute of Quality and Standard for Agro-products, Zhejiang Academy of Agricultural Sciences) ;
  • Zhou, Yan (Key Laboratory of Control Technology and Standard for Agro-product Safety and Quality Ministry of Agriculture, Key Laboratory of Food Quality and Safety of Jiangsu Province-State Key Laboratory Breeding Base, Institute of Food Quality and Safety, Jiangsu Academy of Agricultural Sciences) ;
  • Bao, Hongduo (Key Laboratory of Control Technology and Standard for Agro-product Safety and Quality Ministry of Agriculture, Key Laboratory of Food Quality and Safety of Jiangsu Province-State Key Laboratory Breeding Base, Institute of Food Quality and Safety, Jiangsu Academy of Agricultural Sciences) ;
  • Wang, Ran (Key Laboratory of Control Technology and Standard for Agro-product Safety and Quality Ministry of Agriculture, Key Laboratory of Food Quality and Safety of Jiangsu Province-State Key Laboratory Breeding Base, Institute of Food Quality and Safety, Jiangsu Academy of Agricultural Sciences)
  • Received : 2016.11.07
  • Accepted : 2017.05.21
  • Published : 2017.07.28
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Abstract

The mcr-1 gene is a new "superbug" gene discoverd in China in 2016 that makes bacteria highly resistant to the last-resort class of antibiotics. The mcr-1 gene raised serious concern about its possible global dissemination and spread. Here, we report a potential anti-resistant strategy using the CRISPR/Cas9-mediated approach that can efficiently induce mcr-1 gene knockout in Escherichia coli. Our findings suggested that using the CRISPR/Cas9 system to knock out the resistance gene mcr-1 might be a potential anti-resistant strategy. Bovine myeloid antimicrobial peptide-27 could help deliver plasmid pCas::mcr targeting specific DNA sequences of the mcr-1 gene into microbial populations.

Keywords

References

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