• Journal of Practical Agriculture & Fisheries Research
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• v.4 no.1
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• pp.80-88
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• 2002

This study was carried out to investigate the native distribution and flowering characteristics of Lycoris genus which is endemic species in Asia. This study was summarized as fellows: Native distribution of Lycoris genus was situated in latitude 37- 24 degrees with high humidity of coastline. Mininum temperature of native area was at -10℃ during winter season. The leaf of L. squamigera, L. koreana, L. sangunea, L. sprengeri, L. incanata and L. flavescens emergenced in spring. The leaf of L. radiata, L. rdiata var pumila, L. aurea, L. traubii, L. albiflora and L. houdyshelli emergenced in autum. Bulb of Lycoris genus show a sympodial branching system which is composed of 14-23 scales and 2.8-5.2 leaves per each bulb at flowering time. The flower shape of L. squamigera, L. Koreana, L, aurea, L. incanata, L. sprengeri, L. sanguinea and L. flavescens was trumpet. The flower of L. radiata. L. radiata var pumila, L. albiflora, L. houdchelli and L. traubii. was spider.

• Journal of Practical Agriculture & Fisheries Research
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• v.9 no.1
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• pp.121-132
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• 2007

1. 버섯종균 개선 버섯의 품질과 수량을 좌우하는 주요 요인은 종균의 활력이다. 그러나 라오스에서는 종균의 활력이 낮아 조그마한 100cc 병에 배양완성하는데 14일이 소요되는 등 볍씨종균의 균사생장과 균사밀도가 매우 낮음을 확인하였다. 따라서 100cc 병을 1000cc 비닐봉지로 개선하여 입봉하는데 소요되는 시간을 10배로 단축 개선하였다. 또한 곡립종균을 액체종균으로 대처한 결과 균사생장속도가 2배가 빨랐으며 작업속도가 4배가 빨라졌다. 2. 버섯배지제조 방법 개선 미세하고 건조한 톱밥에 수분을 첨가하는데 50%이하의 수분함량을 유지함으로서 균사가 표면에만 자라고 있는 것을 확인하고 버섯 배지제조 시 물리성을 개선하기 위하여 볏짚을 잘게 잘라 10%~100%까지 혼합하는 실험을 실시하였다. 그 결과 톱밥 50%에 볏짚 50% 혼합하는 것이 균사생장 속도와 밀도가 좋았다. 또한 왕겨도 30%정도 혼합한 배지처리에서 가장 좋았다. 또한 수분함량도 65%로 향상하여 혼합하도록 지도하였다. 3. 버섯배지살균 방법 개선 라오스에서는 왕겨를 태워 살균을 실시하고 있다. 화력이 약하여 살균한 배지에서 볍씨의 새싹이 돋아나오는 경우를 발견하였다. 이는 살균이 제대로 되지 않았음을 의미한다. 그래서 이동식 직화식 살균을 권하였으나 시설자재값이 감당이 안되어 엄두를 내지 못하였다. 따라서 가스버너를 도입하여 단시간에 화력을 높여 살균을 실시하므로서 세균의 증식을 막아 균사생장속도를 빠르게 하고 균사량의 축적을 높이는데 교육을 하고 컨설팅을 실시하여 개선하였다. 4. 병뚜껑과 형성틀을 대체한 링을 이용한 입봉작업시간 단축과 비용절감 버섯봉지에 배지를 담는 입봉작업을 할 때 병뚜껑과 그의 형성틀을 끼우고 솜을 조금 뜯어 톱밥배지 위에 놓는 작업을 한다. 이는 아주 잘못된 방법이다. 왜 그렇게 하는가하는 것은 이해가 간다. 그렇게 하는 이유는 접종할 때 실내에서 그냥 접종하므로 뚜껑을 열때 보호막 역할을 할 것으로 생각하고 그렇게 하는데 천만의 말이다. 어떻든 뚜껑을 열면 잡균이 들어가는 것은 마찬가지이다. 그래서 이 솜을 배지표면에 놓으면 마른 솜이 그렇지 않아도 수분이 적은데 이 솜이 수분을 또 빼앗아 가버린다. 그래서 균사생장이 늘려 15일이면 다 자라야할 균사배양기간이 한달씩 걸려도 표면만 살짝 잘린 결과가 빗어진다. 이렇게 표면만 균사가 사탕 발림식으로 자라면 품질이 저하되고 수량이 적고 병해충에 저항력이 약해지게 된다. 따라서 뚜껑과 형성틀, 솜을 모두 없애고 봉지 상단부위를 U자형으로 꺽어서 링을 끼우는 방법을 실제로 실험으로 보여준 결과 작업능률이 5배로 빠르고 작업공정이 빨라짐으로서 세균번식밀도가 적어 균사생장 속도가 2배로 빨라졌고 수량이 배가됨을 증명하고 보급하여 많은 호평을 받았다.

• Journal of Practical Agriculture & Fisheries Research
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• v.3 no.1
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• pp.85-93
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• 2001

The inter-relationship and combining abilities of characters for leaf length, leaf width, branch number, leaf number, leaf area, fresh weight and dry weight were studied in the F₁ combination of 15 crosses from the partial six-parent diallel cross in Petunia hybrida. The leaf area, fresh weight and dry weight showed high positive correlation together with the other growth characters such as leaf length, leaf width, branch number, leaf number. The mean squares of general combining ability(GCA) and specific combining ability(SCA) were highly significant for all the growth characters. The estimates of variance components of SCA were greater than those of GCA for all the characters, showing preponderance of non-additive gene actions for these characters. GCA effect was high in the lines of D and G for leaf length and fresh weight, and in the lines of C and D for branch number, leaf number, leaf area and fresh weight. The crosses of D×E, D×G, and E×G exhibited high SCA effect for all the growth characters such as leaf length, leaf width, branch number, leaf number, leaf area, fresh weight, and dry weight. The broad sense heritability was generally high compared to narrow sense one in the 7 characters. The highest heritability of the broad and narrow sense was shown in the leaf width and dry weight.

• Journal of Practical Agriculture & Fisheries Research
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• v.7 no.1
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• pp.90-96
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• 2005

Tubers(4-5cm diameter) of Zantedeschia 'Black Magic' were used to study the induction of flowering in GA₃ soaking times and concentration. The GA₃ soaking were 10 seconds and 30 minutes at the GA₃ concentrations of 50, 100, 250, and 500 mg·L¹. The time to emergence in GA₃ treatment were 1.1~4.0 days shorter than control Growth characteristics were good in GA₃ treatment but that was no significance in GA₃ soaking time and concentration. When GA₃ soaking time and concentration were increased, days to flowering was shorter, but flower stalk length, flower stalk width, flower length, flower width were no difference. The number of flower per tuber was most as 4.0~4.3 in 250~500 mg·L¹ GA₃ concentration and that was about 2 times compared to control. The normal flower ratio and bulb enlargement were similar compared with GA₃ soaking time and concentration. GA₃ 250~500 mg·L¹ treatment is necessary for improvement of number of flowers per bulb.

• Journal of Practical Agriculture & Fisheries Research
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• v.25 no.1
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• pp.14-19
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• 2023

In summer, the natural ventilation performance for varying greenhouse width is very important in the glasshouses for year round cultivation. The effect of the side openings and greenhouse width on natural ventilation performance was analyzed by simulation. The necessary ventilation rate with different solar radiation transmittance increased significantly when the outside temperature grows higher. The necessary ventilation rate of 40% transmittance was about half of that of 90% transmittance. In consequence, shading effect on temperature control in greenhouse is significant in summer. When the total area of the openings for ventilation is constant, the maximum ventilation rate happens when the area of roof openings is equal to the area of side openings. This maximum ventilation rate is about 3 times of that of the greenhouse with roof openings and without side openings. Therefore, the side openings are advantageous to improve the natural ventilation in greenhouses. As the greenhouse width increases, the influence of side openings on the ventilation rate is becoming smaller. If the natural ventilation rate of the greenhouse with roof and side openings is to become double of that of the roof openings only, the width should be narrower than 38.4m for the Venlo type and 64m for Wide span type.

• Animal Bioscience
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• v.36 no.8
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• pp.1241-1251
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• 2023

Objective: Egg yolk immunoglobulin (IgY) is an antibiotic alternative to prevent and fight intestinal pathogenic infections. This study aimed to investigate the effects of sodium alginate/chitosan/sodium alginate IgY microcapsules on the growth performance, serum parameters, and intestinal health of broiler chickens. Methods: One-day-old broilers (Ross 308) were divided into five treatments, each with 10 replicates of five chickens. The dietary treatments were maintained for 28 days and consisted of a basal diet (NC), basal diet + 500 mg chlortetracycline/kg diet (CH), basal diet + 50 mg non-microencapsulated IgY/kg diet (NM), basal diet + 600 mg low levels microencapsulated IgY/kg diet (LM), and basal diet + 700 mg high levels microencapsulated IgY/kg diet (HM). Results: Throughout the 28-day trial period, the NM, LM, HM, and CH groups increased average daily gain compared with the NC group (p<0.05), and the HM group reduced feed conversion ratio compared with the CH group (p<0.05). The LM and HM groups increased relative organ weights of thymus and spleen compared with the CH and NM groups (p<0.05). The HM group improved the duodenal, jejunal and ileum villi height (VH) and villus height to crypt depth ratio (VH:CD) compared with the CH and NM groups (p<0.05). Compared with the CH group, the HM group increased serum immunoglobulin (IgA), immunoglobulin G (IgG), superoxide dismutase, total antioxidant capacity, and glutathione peroxidase levels (p<0.05), and decreased serum malondialdehyde levels (p<0.05). Compared with the NC group, the NM, LM, HM, and CH groups reduced colonic Escherichia coli and Salmonella levels (p<0.05). and the HM group promoted the levels of lactic acid bacteria and bifidobacteria compared with the CH group (p<0.05). Conclusion: Microencapsulation could be considered as a way to improve the efficiency of IgY. The 700 mg high levels microencapsulated IgY/kg diet could potentially be used as an alternative to antibiotics to improve the immune performance and intestinal health, leading to better performance of broiler chickens.

• Korean Journal of Environmental Biology
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• v.39 no.4
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• pp.415-422
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• 2021

Cotton is an important fiber crop, and its seeds are used as feed for dairy cattle. Crop biotechnology has been used to improve agronomic traits and quality in the agricultural industry. The frequent unintentional release of LM cotton into the environment in South Korea is attributed to the increased application of living modified (LM) cotton in food, feed, and processing industries. To identify and monitor the LM cotton, a method for detecting the approved LM cotton in South Korea is required. In this study, we developed a method for the simultaneous detection of four LM cotton varieties, MON757, MON88702, COT67B, and GHB811. The genetic information of each LM event was obtained from the European Commission-Joint Research Centre and Animal and Plant Quarantine Agency. We designed event-specific primers to develop a multiplex PCR method for LM cotton and confirmed the specific amplification. Using specificity assay, random reference material(RM) mixture analysis and limit of detection(LOD), we verified the accuracy and specificity of the multiplex PCR method. Our results demonstrate that the method enabled the detection of each event and validation of the specificity using other LM RMs. The efficiency of multiplex PCR was further verified using a random RM mixture. Based on the LOD, the method identified 25 ng of template DNA in a single reaction. In summary, we developed a multiplex PCR method for simultaneous detection of four LM cotton varieties, for possible application in LM volunteer analysis.

• Journal of Korean Society of Forest Science
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• v.112 no.2
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• pp.248-257
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• 2023

Because of various societal changes, such as the recent improvement in income levels and extension of the flexible work system, the demand for forest recreation activities and their use patterns are undergoing a change. Accordingly, it is necessary to identify the characteristics of each type through the segmentation of the overall forest recreation and welfare markets and to plan differentiated policies for each market type. This study classifies the forest recreation and welfare activities according to four types of users (i.e., passive usage type, ordinary type, active lover type, and indifferent type) using the Latent Class Analysis and examines their demographic and socioeconomic characteristics to explain the differences between the groups. Three policy implications were derived from the results obtained: 1) the group experiencing forest recreation welfare is subdivided; 2) the socioeconomic characteristics that distinguish the groups undertaking forest recreation activities were identified; and 3) the policy targets and characteristics that can increase the experience of forest recreation welfare were identified. This study is insightful as it suggests differentiated policies for each group and proposes policy measures to move to the desirable group.

• Animal Bioscience
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• v.36 no.6
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• pp.851-860
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• 2023

Objective: This study aims to evaluate the target genes of gga-miR-20a-5p and the regulated immune responses in the chicken macrophage cell line, HD11, by the exosome-mediated delivery of miR-20a-5p. Methods: Exosomes were purified from the chicken macrophage cell line HD11. Then, mimic gga-miR-20p or negative control miRNA were internalized into HD11 exosomes. HD11 cells were transfected with gga-miR-20a-5p or negative control miRNA containing exosomes. After 44 h of transfection, cells were incubated with or without 5 ㎍/mL poly(I:C) for 4 h. Then, expression of target genes and cytokines was evaluated by quantitative realtime polymerase chain reaction. Results: Using a luciferase reporter assay, we identified that gga-miR-20a-5p directly targeted interferon gamma receptor 2 (IFNGR2), mitogen-activated protein kinase 1 (MAPK1), mitogen-activated protein kinase kinase kinase 5 (MAP3K5), and mitogen-activated protein kinase kinase kinase 14 (MAP3K14). Moreover, the exosome-mediated delivery of gga-miR-20a-5p successfully repressed the expression of IFNGR2, MAPK1, MAP3K5, and MAP3K14 in HD11 cells. The expressions of interferon-stimulated genes (MX dynamin like GTPase 1 [MX1], eukaryotic translation initiation factor 2A [EIF2A], and oligoadenylate synthase-like [OASL]) and proinflammatory cytokines (interferon-gamma [IFNG], interleukin-1 beta [IL1B], and tumor necrosis factor-alpha [TNFA]) were also downregulated by exosomal miR-20a-5p. In addition, the proliferation of HD11 cells was increased by exosomal miR-20a-5p. Conclusion: The exosome-mediated delivery of gga-miR-20a-5p regulated immune responses by controlling the MAPK and apoptotic signaling pathways. Furthermore, we expected that exosomal miR-20a-5p could maintain immune homeostasis against highly pathogenic avian influenza virus H5N1 infection by regulating the expression of proinflammatory cytokines and cell death.

• KSCE Journal of Civil and Environmental Engineering Research
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• v.43 no.3
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• pp.309-319
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• 2023

Recently, the frequency and intensity of meteorological disasters have increased due to climate change. In South Korea, there are regional differences in vulnerability and response capability to cope with climate change because of regional climate characteristics. In particular, drought results from various factors and is linked to extensive meteorological, hydrological, and agricultural impacts. Therefore, in order to effectively cope with drought, it is necessary to use a composite drought index that can take into account various factors, and to evaluate future droughts comprehensively considering climate change. This study evaluated hydrologic risk(${\bar{R}}$) of future drought in the Nakdong River basin based on the Dynamic Naive Bayesian Classification (DNBC)-based composite drought index, which was calculated by applying Standardized Precipitation Index (SPI), Streamflow Drought Index (SDI), Evaporate Stress Index (ESI) and Water Supply Capacity Index (WSCI) to the DNBC. The indices used in the DNBC were calculated using observation data and climate scenario data. A bivariate frequency analysis was performed for the severity and duration of the composite drought. Then using the estimated bivariate return periods, hydrologic risks of drought were calculated for observation and future periods. The overall results indicated that there were the highest risks during the future period (2021-2040) (${\bar{R}}$=0.572), and Miryang River (#2021) had the highest risk (${\bar{R}}$=0.940) on average. The hydrologic risk of the Nakdong River basin will increase highly in the near future (2021-2040). During the far future (2041-2099), the hydrologic risk decreased in the northern basins, and increased in the southern basins.