Development of simultaneous detection method for living modified cotton varieties MON757, MON88702, COT67B, and GHB811
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Il Ryong Kim
(Division of Ecological Safety, National Institute of Ecology(NIE))
Min-A Seol (Division of Ecological Safety, National Institute of Ecology(NIE)) A-Mi Yoon (Division of Ecological Safety, National Institute of Ecology(NIE)) Jung Ro Lee (Division of Ecological Safety, National Institute of Ecology(NIE)) Wonkyun Choi (Division of Ecological Safety, National Institute of Ecology(NIE)) |
1 | Animal and Plant Quarantine Agency. 2021. Quarantine Agency Notice. Animal and Plant Quarantine Agency. Gimcheon, Korea. Available from: http://www.qia.go.kr/bbs/lawAnn/viewLawWebAction.do?id=183929&type=0 (accessed on 1 September 2021). |
2 | Akbar W, RS Brown, WC Burns, TL Clark, A Gowda, A Pan, X Shi, JW Stelzer and K Wu. 2017. U.S. Patent No. US 2017/0166922 A1. U.S. Patent and Trademark Office. Washington, DC. |
3 | Ali ME, MA Razaak and SB Abd Hamid. 2014. Multiplex PCR in species authentication: Probability and Prospects - a review. Food Anal. Methods 7:1933-1949. DOI |
4 | Brownie J, S Shawcross and J Theaker. 1997. The elimination of primer-dimer accumulation in PCR. Nucleic Acids Res. 25:3235-3241. DOI |
5 | Cervantes FA, EA Backus, L Godfrey, MG Rojas, W Akbar and TL Clark. 2019. Quantitative differences in feeding behavior of Lygus lineolaris (Hemiptera: Miridae) on transgenic and nontransgenic cotton. J. Econ. Entomol. 112:1920-1925. DOI |
6 | Choi W, IR Kim, HS Lim and JR Lee. 2020. A multiplex PCR method for the detection of genetically modified alfalfa (Medicago sativa L.) and analysis of feral alfalfa in South Korea. PNIE 1:83-89. |
7 | Eum SJ, IR Kim, HS Lim, JR Lee and W Choi. 2019. Eventspecific multiplex PCR method for four genetically modified cotton varieties, and its application. Appl. Biol. Chem. 62:52. |
8 | Huseth AS, DA D'Ambrosio and GG Kennydy. 2020. Understanding the potential impact of continued seed treatment use for resistance management in Cry51Aa2.834_16 Bt cotton against Frankliniella fusca. PLoS One 15:e0239910. |
9 | ISAAA. 2019. Global Status of Commercialized Biotech/GM Crops in 2019: Biotech Crops Drive Socio-Economic Development and Sustainable Environment in the New Frontier. ISAAA Brief No. 55. International Service for the Acquisition of Agri-biotech Applications. Ithaca, NY. |
10 | Jansens S, R Dreesen, W Aartsen, J Vanhaelen, H Moser and G Light. 2017. U.S. Patent No. WO2017182420A1. U.S. Patent and Trademark Office. Washington, DC. |
11 | Jo BH, MA Seol, SY Shin, IR Kim, W Choi, SJ Eum, HR Song and JR Lee. 2016. Multiplex PCR method for environmental monitoring of approved LM cotton events in Korea. J. Plant Biotechnol. 43:91-98. |
12 | JRC. 2021. European Union Reference Laboratory for Genetically Modified Food and Feed. European Commission, Joint Research Centre. Brussels, Belgium. Available from: http://gmo-crl.jrc.ec.europa.eu(accessed on 1 September 2021). |
13 | KBCH. 2021. KBCH Trend Report. Korea Biosafety Clearing House. Available from: http://biosafety.or.kr/ (accessed on 1 September 2021). |
14 | Kim DW, IR Kim, HS Lim, W Choi and JR Lee. 2019. Development of a multiplex PCR assay to monitor living modified cottons in South Korea. Appl. Sci. 9:2688. |
15 | Kim IR, HS Lim, W Choi, DI Kang, SY Lee and JR Lee. 2020. Monitoring living modified canola using an efficient multiplex PCR assay in natural environments in South Korea. Appl. Sci. 10:7721. |
16 | Kim YJ, S Kloos, J Romeis and M Meissle. 2021. Effects of mCry51Aa2-producing cotton on the non-target spider mite Tetranychus urticae and the predatory bug Orius majusculus. J. Pest Sci. 94:351-362. DOI |
17 | Markoulatos P, N Siafakas and M Moncany. 2002. Multiplex polymerase chain reaction: A practical approach. J. Clin. Lab. Anal. 16:47-51. DOI |
18 | NIE. 2019. Establishment of Detection Method for LMO. National Institute of Ecology. Seocheon, Korea. |
19 | NIE. 2020a. Establishment of Detection Method for LMO. National Institute of Ecology. Seocheon, Korea. |
20 | NIE. 2020b. Study on Environmental Monitoring and Post-management of LMO. National Institute of Ecology. Seocheon, Korea. |
21 | Park JH, MA Seol, SJ Eum, IR Kim, HS Lim, JR Lee and W Choi. 2020. Development of a multiplex PCR method for identification of four genetically modified maize lines and its application in living modified organism identification. J. Plant Biotechnol. 47:309-315. |
22 | Shin SY, HS Lim, MA Seol, YJ Jung, IR Kim, HR Song, JR Lee and W Choi. 2016. Four multiplex PCR sets of 11 LM Maize for LMO environmental monitoring in Korea. J. Plant Biotechnol. 43:473-478. DOI |
23 | Wu Y, J Li, X Li, S Zhai, H Gao and Y Li. 2019. Development and strategy of reference materials for the DNA-based detection of genetically modified organisms. Anal. Bioanal. Chem. 411:1729-1744. DOI |
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