• Journal of Microbiology and Biotechnology
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• v.23 no.12
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• pp.1757-1764
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• 2013

Trichoderma harzianum ZF-2 producing laccase was isolated from decaying samples from Shandong, China, and showed dye decolorization activities. The objective of this study was to optimize its culture conditions using a statistical analysis of its laccase production. The interactions between different fermentation parameters for laccase production were characterized using a Plackett-Burman design and the response surface methodology. The different media components were initially optimized using the conventional one-factor-at-a-time method and an orthogonal test design, and a Plackett-Burman experiment was then performed to evaluate the effects on laccase production. Wheat straw powder, soybean meal, and $CuSO_4$ were all found to have a significant influence on laccase production, and the optimal concentrations of these three factors were then sequentially investigated using the response surface methodology with a central composite design. The resulting optimal medium components for laccase production were determined as follows: wheat straw powder 7.63 g/l, soybean meal 23.07 g/l, $(NH_4)_2SO_4$ 1 g/l, $CuSO_4$ 0.51 g/l, Tween-20 1 g/l, $MgSO_4$ 1 g/l, and $KH_2PO_4$ 0.6 g/l. Using this optimized fermentation method, the yield of laccase was increased 59.68 times to 67.258 U/ml compared with the laccase production with an unoptimized medium. This is the first report on the statistical optimization of laccase production by Trichoderma harzianum ZF-2.

• Journal of Animal Reproduction and Biotechnology
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• v.35 no.1
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• pp.2-11
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• 2020

Assisted reproductive technologies (ART) merely depend on improving the oocyte maturation and their developmental competence to produce good quality embryos. Oocyte maturation passes through long and complex molecular steps starts from the early embryonic life and ends with sperm fertilization. Oocyte developmental competence can be attained by improving the nuclear and cytoplasmic mechanisms together with some epigenetic maturation. In this review, we highlight the cornerstones of oocyte maturation on both nuclear and cytoplasmic levels. Interfering or supporting these molecular mechanisms would help in the development of novel regulating agents for reproductive performance of humans and livestock species.

• Proceedings of the Korean Society for Agricultural Machinery Conference
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• 1993.10a
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• pp.98-107
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• 1993

Domestic production of agricultural machinery has come to be seen as appropriate entry route in the capital goods industry for most African countries, including Ghana, which has purely and strongly agricultural -based economics with a weak industrial infrastructure. Recent studies in Ghana do indicate that in addition to possessing technological capability in the manufacture of handtools, there is evidence of increased production of machinery for the primary food processing sector. Local manufacture of tractors and associated implements is embryonic, with an average domestic production of agricultural machinery in Ghana and analyses the available technologies and economic indicators in the local agricultural machinery industry. Areas for indicators in the local agricultural machinery industry. Areas for future development are identified.

• Agricultural and Biosystems Engineering
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• v.5 no.2
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• pp.40-44
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• 2004

This study was conducted to solve the problem of spinach harvesting done by manpower at the outdoor field during the cold winter season. Prototype spinach harvester was designed to dig, pick-up, and collect in a continuous operation for harvesting outdoor field-planted crawling type spinach. In the field test, two types of blades (Type A : angle of $150^{\circ}$, Type B : straight) were used for measuring the cutting loads of spinach and chain conveyor with lugs was used for picking up the root cut spinach. Prototype's vibrating blade reduced the digging power of the fixed blade by $46\%$. The loss was also very little ($0.7\%$) with a digging depth of 4 cm, an oscillation frequency of 748 rpm, and an oscillation distance of 33 mm. The working performance of the prototype spinach harvester was 38 hour/ha resulting to $96\%$ labor cost reduction compared to the conventional harvesting.

• Journal of Microbiology and Biotechnology
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• v.29 no.1
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• pp.160-170
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• 2019

The lactic acid bacteria species Lactobacillus plantarum (L. plantarum) has been used extensively for vaccine delivery. Considering to the critical role of dendritic cells in stimulating host immune response, in this study, we constructed a novel CD11c-targeting L. plantarum strain with surface-displayed variable fragments of anti-CD11c, single-chain antibody (scFv-CD11c). The newly designed L. plantarum strain, named 409-aCD11c, could adhere and invade more efficiently to bone marrow-derived DCs (BMDCs) in vitro due to the specific interaction between scFv-CD11c and CD11c located on the surface of BMDCs. After incubation with BMDCs, the 409-aCD11c strain harboring a eukaryotic vector pValac-GFP could lead to more efficient expression of GFP compared with wild-type strains shown by flow cytometry analysis, indicating the enhanced translocation of pValac-GFP from L. plantarum to BMDCs. Similar results were also observed in an in vivo study, which showed that oral administration resulted in efficient expression of GFP in both Peyer's patches (PP) and mesenteric lymph nodes (MLNs) within 7 days after the last administration. In addition, the CD11c-targeting strain significantly promoted the differentiation and maturation of DCs, the differentiation of $IL-4^+$ and $IL-17A^+$ T helper (Th) cells in MLNs, as well as production of $B220^+$ $IgA^+$ B cells in the PP. In conclusion, this study developed a novel DC-targeting L. plantarum strain which could increase the ability to deliver eukaryotic expression plasmid to host cells, indicating a promising approach for vaccine study.

• Agricultural and Biosystems Engineering
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• v.1 no.1
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• pp.59-62
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• 2000

After ten years of implementing the agricultural automation program in Taiwan, some positive effects and satisfactory results have been recognized by both the agricultural industry and local administrative bureaux. The automation of agriculture is a response to sophisticated demands for production and quality in countries with high labor costs. The development of sensor systems, control systems, precision agriculture systems, and engineering for plant culture systems will determine the degree of automation used for crop production in the 21st century. The engineering system will capitalize upon expertise from physiologists, pathologists, systems analysts, agronomists, horticulturists, computer programmers, economists, crop producers and managers in order to efficiently implement automated crop production.

• Proceedings of the Korean Society for Agricultural Machinery Conference
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• 1993.10a
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• pp.158-173
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• 1993

The formulated mechanization packages for grain maize production have performed to the expected limit generating encouraging information. Besides physical feasibility , management factors viz ; production operation sequence, operations scheduling and machinery matching with respect to environment can still limit system suitability. A new production operation sequence was introduced to overcome weed problems and limitations of available working days. Proper operations scheduling will improve the initial soil-crop environment for better seedling establishment, and reduce the (). been identified as key factors to reduce capital investment and cost of proudction .

• Korean Journal of Plant Resources
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• v.22 no.6
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• pp.518-521
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• 2009

High productivity of ginger (Zingiber officinale Roscoe) was obtained from the rhizome produced by shoot-tip culture with Korean native variety, Seosanjong. Seed rhizomes induced by shoot-tip culture were successfully established in the field. The rhizomes induced by both plant or rhizome were higher in emergence rate and faster in days to emergence than those of home seed production. The seed rhizome production induced by shoot-tip culture was two times heavier than that of home seed production. These results suggest that shoot-tip culture might be one of mass propagation methods in seed rhizome of ginger plant.

• Journal of the Korean association of regional geographers
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• v.12 no.6
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• pp.748-756
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• 2006

This study mainly deals with price behavior developed in a agricultural location model (or closed model) considering the production and demand aspects. The short-run situation of price and output is associated with the yearly fluctuation of yield from agricultural production. Demand is generally regarded as constant in the short-run because of being inelastic over short time. The long-run situation is associated with a period in which all related variables can be varied. Then a price behaviors from the two contrasting closed models have been further explored in the long-run economy. Agricultural price for each activity in the closed model is affected by change in agricultural production. Also, falling agricultural price is connected with lower rents and lower land values.

• Asian-Australasian Journal of Animal Sciences
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• v.21 no.5
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• pp.628-632
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• 2008

Egg production traits are economically important both for egg-laying and broiler lines of chicken. In sheep, the Q249R mutation in BMPR-IB is associated with ovulation rate. The present study cloned a partial chicken BMPR-IB fragment which contained the corresponding ovine Q249R mutation, including partial exon 6 and exon 7 and full-length intron 6. Five nucleotide changes were identified by alignment of the fragment amplified from Jining Bairi and Zang chickens. Among these nucleotide substitutions, the C/T transition at the base position of 35 and the A/G transition at the base position of 287 were found to be highly polymorphic, and named as SNPs C35T and A287G, respectively. For the SNP C35T, 331 hens of a synthetic broiler line were genotyped by a PCR-SSCP approach and allele C was found to be dominant. For the SNP A287G, 604 birds from the synthetic broiler line, a commercial egg-laying line, as well as three Chinese indigenous chicken breeds were genotyped by a PCR-RFLP technique. The associations of these two SNPs with egg production traits in the broiler line were analyzed. The results indicated that both the C35T and the A287G SNPs were not associated with egg production at 33wks and from 33wks to 42 wks (p>0.1), whereas the SNP A287G was associated with egg production from 47 to 56 wks (p<0.05). The dominance genetic effects on this latter trait and on egg production from 33 to 42 wks were significant (p<0.05).