• Journal of Ginseng Research
• /
• v.43 no.1
• /
• pp.10-19
• /
• 2019

Background: Frequent overdose of paracetamol (APAP) has become the major cause of acute liver injury. The present study was designed to evaluate the potential protective effects of ginsenoside Rk1 on APAP-induced hepatotoxicity and investigate the underlying mechanisms for the first time. Methods: Mice were treated with Rk1 (10 mg/kg or 20 mg/kg) by oral gavage once per d for 7 d. On the 7th d, allmice treated with 250mg/kg APAP exhibited severeliverinjury after 24 h, and hepatotoxicitywas assessed. Results: Our results showed that pretreatment with Rk1 significantly decreased the levels of serum alanine aminotransferase, aspartate aminotransferase, tumor necrosis factor, and interleukin-$1{\beta}$ compared with the APAP group. Meanwhile, hepatic antioxidants, including superoxide dismutase and glutathione, were elevated compared with the APAP group. In contrast, a significant decrease in levels of the lipid peroxidation product malondialdehyde was observed in the ginsenoside Rk1-treated group compared with the APAP group. These effects were associated with a significant increase of cytochrome P450 E1 and 4-hydroxynonenal levels in liver tissues. Moreover, ginsenoside Rk1 supplementation suppressed activation of apoptotic pathways by increasing Bcl-2 and decreasing Bax protein expression levels, which was shown using western blotting analysis. Histopathological observation also revealed that ginsenoside Rk1 pretreatment significantly reversed APAP-induced necrosis and inflammatory infiltration in liver tissues. Biological indicators of nitrative stress, such as 3-nitrotyrosine, were also inhibited after pretreatment with Rk1 compared with the APAP group. Conclusion: The results clearly suggest that the underlying molecular mechanisms in the hepatoprotection of ginsenoside Rk1 in APAP-induced hepatotoxicity may be due to its antioxidation, antiapoptosis, anti-inflammation, and antinitrative effects.

• Journal of Apiculture
• /
• v.32 no.4
• /
• pp.391-394
• /
• 2017

Propolis is made by bees collecting protective material or essence of plants and mixing with saliva and enzymes produced by the salivary glands. It is used to repair the inside of the honeycomb, keep it sterile, and adjust the temperature and humidity. Propolis is a natural antibiotic substance that it is used to make a clean room by coating the cell before the queen bee lay eggs, and preventing the bacteria from invading by using with wax when sealing the nursery room. Propolis extract is a health functional food with antioxidant and oral antimicrobial effects. In order to use propolis in food, its active ingredients are extracted with ethanol. Water-soluble propolis was prepared by mixing and stirring honey and ethanol extracted propolis (EEP) solution. When 1kg of honey and 100ml of ethanol extracted propolis solution were mixed and stirred, the total flavonoid content of water-soluble propolis was $6.6{\pm}1.1mg/10g$, and the free radical scavenging effects of water-soluble propolis were 54 to 74%.

• Proceedings of the Korean Society for Agricultural Machinery Conference
• /
• 2000.11b
• /
• pp.132-138
• /
• 2000

Complex gear shifting and high speed-reduction ratio reduce the transmission efficiency in power driveline of agricultural tractors. According to a field test, the power transmission efficiency of a tractor in transporting operations was estimated about 70%. However, the actual efficiency was found by the experiment to fluctuate in a range of 56 to 87%. Therefore, the constant efficiency model commonly used for a simulation of power drivelines is not likely to simulate its performance more accurately. In order to predict power transmission efficiency more accurately, a new model was proposed and the new concepts of the maximum efficiency and sticking torque were introduced. The error mean between the measured and the predicted efficiencies was about 2.3% in mean. The new model reflecting the transmission characteristics in the power driveline of tractors could be used to analyze and predict the power transmission performance of tractors more accurately.

• The Korean Journal of Pesticide Science
• /
• v.17 no.1
• /
• pp.41-49
• /
• 2013

This study was performed to investigate the cultivation pattern and perception, such as safety awareness, of environmentally-friendly organic materials among Korean farmers. The main reason for participation of the environmentally-friendly agriculture was for the production of safe agricultural crops. Compared to small-acreage farmer were more aware about the safety of environmentally-friendly organic materials to the subjects. Accordingly, 23.6% among the respondents with environmentally-friendly agriculture certification experienced the adverse effect by using the environmentally-friendly organic materials, compared to 33.8% of among the respondent without the certification. The experienced type of adverse effects consisted mainly 'eyes itch', 'hands and feet itch' and 'fatigue'. Also, respondent experiencing the adverse effects by using the environmentally-friendly organic materials more comply with the usage standard as how to use marked in wrapping paper, compared to the respondent without experiencing the adverse effects, which indicate that the compliance with the usage standard as how to use marked in wrapping paper is closely related the experience the adverse effects. Further study should be needed for more precise investigation of comment for the farmers in order to suggest the effective improvement in terms of policy or education for their safety.

• Journal of Sericultural and Entomological Science
• /
• v.52 no.1
• /
• pp.10-15
• /
• 2014

Regenerated Antheraea pernyi silk sponge was prepared using calcium nitrate 4 hydrate melt and examined the conformational changes treated with aqueous ethanol solution. The conformation of silk sponges was changed from random coil structure to ${\beta}$-sheet and ${\alpha}$-helix conformation with low ethanol concentration (50 ~ 70%). On the other hand, that of silk sponges with 80% ethanol treatment showed ${\beta}$-sheet ($700cm^{-1}$), ${\alpha}$-helix ($625cm^{-1}$), and random coil ($660cm^{-1}$) specific peaks. Wound healing effect in vitro was observed by cytoslective wound healing kit. Therefore, regenerated Antheraea pernyi silk sponges might be used as promising wound dressing materials.

• Journal of Agricultural Extension & Community Development
• /
• v.2 no.2
• /
• pp.191-203
• /
• 1995

One of the major functions of rural extension services is to transfer agricultural technologies and information, and advanced new agricultural techniques developed by research institutes, which are meaningful when they are transferred to farmers for practicl application. Information materials can be transferred in the form of newspapers, radio and television broadcasting, printed materials, audio-visual aids, and public communication networks. Agricultural information systems in the era of localization should be oriented toward county extension services, and the following points should be emphasized for more effective dissemination of agricultural technologies : 1) Central organization of the Rural Development Administration should put more emphasis on the production and dissemination of agricultural information to support activities of extension agents at the county level. 2) An Agricultural information center should be established for more effective collection, analysis, processing, production and dissemination of various agri-related information. 3) An advanced and unified network system should be adopted for more accuate and rapid information flow throughout the country, and reinforcement of manpower and facility at the county level should be emphasized for more effective dissemination of agricultural information.

• International Journal of Industrial Entomology and Biomaterials
• /
• v.23 no.2
• /
• pp.193-199
• /
• 2011

Silk protein and agarose are widely known as biocompatible materials in the human body. A three-dimensional (3D) scaffold composed of agarose and low-molecular- weight silk fibroin (LSF) was fabricated and examined in terms of structural characteristics and cellular responses in bone tissue engineering. This study showed that mouse pluripotent precursor cells attached to and proliferated uniformly on and within the LSF-containing 3D scaffold. Interestingly, cell proliferation and attachment was shown to be higher in a 3D scaffold containing 0.02% LSF, as compared to other LSF concentrations. The results of this study suggest that agarose-LSF scaffolds may be useful materials for tissue engineering.

• Proceedings of the Korean Society of Sericultural Science Conference
• /
• 2003.10a
• /
• pp.97-98
• /
• 2003

Current study was aimed to understand an interaction between Dopa decarboxylase (DDC) and proteins that specifically binds to DDC in the silkworm, Bombyx mori. Materials and Methods: Materials-Animal: Bombyx mori Construction of GST fusion protein Preparation of lysates: Protein extracted from whole body of Bombyx mori Methods-In vitro binding assay with lysates, Peptide sequence and RACE-PCR (omitted)

• Korean Journal of Agricultural Science
• /
• v.35 no.1
• /
• pp.53-67
• /
• 2008

The principle goal of environmentally-friendly agriculture is to maintain and conserve water and agriculture environment including drinking water resources by properly using agricultural materials such as agricultural chemicals, chemical fertilizers, and other agricultural materials according to act 19 of foster law of environmentally-friendly agriculture. To achieve these goals, we have to establish Integrated Nutrient Management(INM) and Integrated Pesticide Management(IPM) which are most important core technologies for environmentally-friendly rice cultivation. However, there are lack of criteria and technology for evaluation category according to soil management and its soil classes to practice an environmentally-friendly agriculture. Therefore, we should eatablish the standards to produce the safe agricultural products based on the soil physical and chemical characteristics which are basic properties of soil to accomplish the principle aims of environmentally-friendly agriculture.

• Journal of Agricultural Extension & Community Development
• /
• v.17 no.2
• /
• pp.185-212
• /
• 2010

Agricultural high schools has been changing themselves toward more effective education system to survive in rapidly changing society. Changes occur in new students, in objects of school, and in curriculum. The purpose of this study was to analyze the condition of career education in agriculture high school. Questionnaires were use to collect data from 894 agricultural high schools. Results of this study show that the career education in agriculture high school has many problems in its curriculum, its contents, and the public relation of educational materials. It is recommended that various materials and manuals for career education should be developed and implemented for effective career education in agricultural high school.