• Asian-Australasian Journal of Animal Sciences
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• 제24권1호
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• pp.1-8
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• 2011

Large quantities of high-quality recipient oocytes with uniform cytoplasm are needed for research in the promising field of somatic cell nuclear transfer (SCNT) and embryonic stem cell research. In canines, however, it is difficult to obtain large quantities of oocytes because each donor produces a limited number of mature oocytes in vivo. Although in vitro maturation (IVM) is considered an alternative approach to oocyte production, this technique is still too rudimentary to be used for the production of highquality, uniform oocytes in large quantities. One technique for overcoming this difficulty is to use oocytes obtained from different species. This technique is known as interspecies SCNT (iSCNT). This review provides an overview of recent advances in canine - porcine interspecies SCNT.

• 한국농공학회논문집
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• 제51권1호
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• pp.11-20
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• 2009

Flow pump technique was used in order to determine the soil-water characteristic curve of weathered granite soils in Pocheon area. This technique enables measurement to be more convenient and accurate as it is based on the CU condition of triaxial compression test. Besides, it is also able to measure dry and moisture curves continuously since the test is controled by means of a computer automatically. In this study, not only a hydraulic conductivity of weathered granite soils at fully saturated state in Pocheon area, but also a soil-water characteristic curve throughout unsaturate flow tests were determined. In addition, Brooks and Corey's model and Genuchten's model were used to simulate the soil-water characteristic curve. On the basis of the simulation an unsaturate hydraulic conductivity was predicted.

• 한국농공학회:학술대회논문집
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• 한국농공학회 2001년도 학술발표회 발표논문집
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• pp.340-343
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• 2001

The main objective of this study is to simulate design flood discharge of the Sungjoo basin. GIS and HEC-HMS were used in this study. GIS technique can extract various hydrological factors from D.E.M(Digital Elevation Model) and the parameters extracted from each watershed were applied to the HEC-HMS. As a result of this study, GIS technique is useful to tile extraction of watershed characteristics factors and HEC-HMS is successful in tile simulation of design flood discharge.

• 한국농공학회:학술대회논문집
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• 한국농공학회 1999년도 Proceedings of the 1999 Annual Conference The Korean Society of Agricutural Engineers
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• pp.618-624
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• 1999

For many soft ground embankment projects , instrumentation programs for stability and settlement management is being essential . This usually leads to generate large volume of data, which can be used for further research. Database technique is most effective method for data management . Data produced by soft ground embankment instrumentation can not be used by itself but must be reproduced using geotechnical analysis technique. In this study, a intelligent database system for softground called IDSIM was developed to examine applicability intellgent database. . The IDSIM analysis instrumentation data automatically and present results by Web/DB interface successfully.

• 한국토양비료학회지
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• 제44권6호
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• pp.1035-1041
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• 2011

Salinity of soil under the plastic film houses in Korea is known as a significant factor to lower the crop production and to hamper the sustainable agricultural land management. In this study we propose a field monitoring technique to examine the methods applied to minimize the adverse effect of salts in soil based on the relationship between soil electrical characteristics and soil properties. Field experiments for 4 different treatments (water only, fertilizer only, DTPA only, and DTPA and fertilizer together) were conducted on soils at the plastic film house built for cultivating a cucumber plant located at Chunan-si, Chungchungnam-do in Korea. The electrical resistivity was measured by both a dipole-dipole and wenner multi-electrodes array method. After the electrical resistivity measurement we also measured the soil water content, temperature, and electrical conductivity on surface soil. The resulted image of the interpreted resistivity by the inversion technique presented a unique spatial distribution depending on the treatment, implying the effect of the different chemical components. It was also highly suspected that resistivity response changed with the nutrients level, suggesting that our proposed technique could be the effective tool for the monitoring soil water as well as nutrient during the cropping period. Especially, subsoils under DTPA treatment at 40 to 60 cm depth typically presented lower soil water accumulation comparing to subsoils under non-DTPA treatment. It is considered that DTPA resulted in increase of a root water uptake. However, our demonstrated results were mainly based on qualitative comparison. Further experiments need to be conducted to monitor temporal changes of electrical resistivity using time lapse analysis, providing that a plant root activity difference based on changes of soil water and nutrients level in time.

• Journal of Biosystems Engineering
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• 제27권4호
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• pp.341-348
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• 2002

Quality evaluation of red ginsengs is determined by outer shape and inner qualities. Especially, the inner qualities are main grading criteria. Currently, red ginsengs are classified into 3-grades; heaven, earth and good. The best heaven grade must not include inner holes and sponge tissues. This study was conducted to develop a red ginseng sorting system using x-ray image processing technique. Because of lens characteristic, gray values of the central region in the x-ray image are higher and gradually decreased towards the edge regions. This difference of gray values gives trouble in segmentation and detection of inner holes in red ginseng image, so preprocessing technique is necessary. The preprocessing was done by subtracting source image from an empty background image. But, simple subtraction was not quite appropriate because of too small contrast between inner holes and sound part. Scaled subtraction images were obtained by multiplying all gray values by some numbers. However this method could not help to set threshold value because the gray values of root part are generally lower than body part when red ginseng is exposed to the x-ray. To determine threshold value for detecting inner holes, an algorithm was developed by increasing overall gray values of less clear images.

• Applied Biological Chemistry
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• 제34권3호
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• pp.279-286
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• 1991

우리의 환경(環境) 중(中)에서 난분해성(難分解性) 공해물질(公害物質) TCAB의 미생물(微生物)에 의(依)한 분해(分解) 가능성(可能性)을 연구(硏究)하기 위하여 enrichment technique에 의하여 4종(種)의 미생물(微生物)을 분리(分離)하였다. 이들 균주(菌株)들은 Achromobactor group VD, Pseudomonas alcaligenes, Moraxella spp., 그리고 Alcaligenes faecalis로 각각 동정(同定)되었다. 또한 이들 미생물(微生物)들은 $MM_2$ 무기배지(無機培地)에서 TCAB를 유일(唯一)한 탄소원(炭素源)으로 이용(利用)하였다.

• 농업과학연구
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• 제45권4호
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• pp.823-837
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• 2018

The objective of this study was to evaluate the firmness and the sweetness index (SI) of tomatoes with a hyperspectral imaging (HSI) technique within the wavelength range of 1000 - 1550 nm. The hyperspectral images of 95 tomatoes were acquired with a push-broom hyperspectral reflectance imaging system, from which the mean spectra of each tomato were extracted from the regions of interest. The reference firmness and sweetness index of the same sample was measured and calibrated with their corresponding spectral data by partial least squares (PLS) regression with different preprocessing methods. The calibration model developed by PLS regression based on the Savitzky-Golay second-derivative preprocessed spectra resulted in a better performance for both the firmness and the SI of the tomatoes compared to models developed by other preprocessing methods. The correlation coefficients ($R_{pred}$) were 0.82, and 0.74 with a standard error of prediction of 0.86 N, and 0.63, respectively. Then, the feature wavelengths were identified using a model-based variable selection method, i.e., variable importance in projection, from the PLS regression analyses. Finally, chemical images were derived by applying the respective regression coefficients on the spectral image in a pixel-wise manner. The resulting chemical images provided detailed information on the firmness and the SI of the tomatoes. The results show that the proposed HSI technique has potential for rapid and non-destructive evaluation of firmness and the sweetness index of tomatoes.

• 한국양봉학회지
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• 제33권4호
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• pp.261-268
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• 2018

Queen honey bees (Apis cerana) can be reared artificially on demand by the use of grafting technique. The technique consists of grafting young worker larvae into queen cell cups and raising in a queenless strong colony. As the age of grafted larvae for queen rearing exhibited several aspects related to quality and reproductive potential of queen, this study were conducted to investigate the influence of age of grafted larva on morphological characteristics and lifespan of queen, and the growth of colony she headed. Our results demonstrated that queens reared from young worker larvae (i.e., less than 1-day old larvae) were significantly larger in size (i.e., body weight and thorax width) than that of queens reared from 2-day old worker larvae. Moreover, queens reared from younger worker larvae initiated egg-laying earlier, stored more spermatozoa in spermatheca and had a longer lifespan compared to queens raised from older worker larvae. We also found a significant positive effect of queen grafting age on the production of worker and drone brood, adult worker population in colonies headed by queens reared from younger larvae. These findings suggested that rearing queens from brood grafted at the earliest possible age could increase the reproductive potential of queen as well as fitness of colony she head.

• Asian-Australasian Journal of Animal Sciences
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• 제17권3호
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• pp.379-385
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• 2004

Two trials in vivo and in vitro were conducted, in vivo to determine the apparent digestibility of gross energy, crude protein, dry matter, acid detergent fiber, neutral detergent fiber and apparent digestible energy in 10 corn by-products. In vivo the diets included one basal corn diet, four corn gluten meal diets, four corn distillers dried grains with solubles diets and two corn distillers dried grains diets using the different methods, 12 crossbred barrows weigh $40{\pm}$1.6 kg were allocated into individual metabolic crate, according to a $6{\times}6$ Latin square design. In vitro using flask technique, filter bag technique and dialysis tubing technique, the digestibilities of gross energy, crude protein and dry matter in corn gluten meal and corn distillers dried grains with solubles were investigated. Pepsin, pancreatin, intestinal fluid, rumen fluid and cellulase were used in incubation. The results showed that correlation coefficient was 0.73 in corn distillers dried grains with solubles between the digestibility of crude protein and acid detergent fiber in vivo (p<0.01); and correlation coefficient was 0.68 in corn distillers dried grains with solubles between the digestibility of gross energy and neutral detergent fiber in vivo (p<0.01). Apparent digestible energy (DE) of corn by-products in pig total tract was predicted by the percentage of crude protein (CP) and the content of gross energy (GE) in feedstuff. The equation: DE=5,601.09+26.69$\times$CP %-0.5904$\times$GE, ($R^2=0.72$). In vitro, filter bag technique was more convenient; furthermore, the digestibility for the treatments (pepsin+pancreatin+rumen fluid and pepsin+pancreatin+cellulase) was better.