• The Plant Pathology Journal
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• 제33권3호
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• pp.276-287
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• 2017

RcsA is a positive activator of extracellular polysaccharide (EPS) synthesis in the Enterobacteriaceae. The rcsA gene of the soft rot pathogen Pantoea sp. strain PPE7 in Pleurotus eryngii was cloned by PCR amplification, and its role in EPS synthesis and virulence was investigated. The RcsA protein contains 3 highly conserved domains, and the C-terminal end of the open reading frame shared significant amino acid homology to the helix-turn-helix DNA binding motif of bacterial activator proteins. The inactivation of rcsA by insertional mutagenesis created mutants that had decreased production of EPS compared to the wild-type strain and abolished the virulence of Pantoea sp. strain PPE7 in P. eryngii. The Pantoea sp. strain PPE7 rcsA gene was shown to strongly affect the formation of the disease symptoms of a mushroom pathogen and to act as the virulence factor to cause soft rot disease in P. eryngii.

• The Plant Pathology Journal
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• 제29권2호
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• pp.144-153
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• 2013

Diverse bacteria are known to colonize plants. However, only a small fraction of that diversity has been evaluated for their biopesticide potential. To date, the criteria for sampling and selection in such bioprospecting endeavors have not been systematically evaluated in terms of the relative amount of diversity they provide for analysis. The present study aimed to enhance the success of bioprospecting efforts by increasing the diversity while removing the genotypic redundancy often present in large collections of bacteria. We developed a multivariate sampling and marker-based selection strategy that significantly increase the diversity of bacteria recovered from plants. In doing so, we quantified the effects of varying sampling intensity, media composition, incubation conditions, plant species, and soil source on the diversity of recovered isolates. Subsequent sequencing and high-throughput phenotypic analyses of a small fraction of the collected isolates revealed that this approach led to the recovery of over a dozen rare and, to date, poorly characterized genera of plant-associated bacteria with significant biopesticide activities. Overall, the sampling and selection approach described led to an approximately 5-fold improvement in efficiency and the recovery of several novel strains of bacteria with significant biopesticide potential.

• 한국농업기계학회:학술대회논문집
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• 한국농업기계학회 1996년도 International Conference on Agricultural Machinery Engineering Proceedings
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• pp.1081-1089
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• 1996

In Korean agriculture, an automatic environment control system for greenhouse is essential to save labor and to increase the quality of products. The existing environment monitoring systems have weighed on greenhouse growers and researchers because of their high cost and difficult applications. Many sensors are widely used for monitoring the greenhouse environment, but most of commercial sensors are expensive and not suitable for use in greenhouses. Thus , the development of an environment monitoring system for exclusive use in greenhouses is essential . The objective of this study was to develop modular environment monitoring systems, which are low-cost , reliable and easy -to -use. The results showed that the sensors for indoor and outdoor environments and nutrient solution had the ranges and accuracies appropriate for use in greenhouses. Also the modular environment systems developed showed a satisfactory performance in terms of stability and reliability in the measurement and acquisition of the greenhouse environment data.

• 한국농업기계학회:학술대회논문집
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• 한국농업기계학회 1999년도 국제 심포지엄 Proceedings of International Symposium on 친환경농업과 기계화방향
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• pp.111-135
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• 1999

• 한국토양비료학회지
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• 제49권5호
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• pp.440-448
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• 2016

Nonpoint sources of pollution (NPS) is defined as diffuse discharges of pollutants (e.g., nutrient, pesticide, sediment, and enteric microorganism) throughout the natural environment and they are associated with a variety of farming practices. Previous studies found that water soluble anionic polyacrylamide (PAM) is one of the highly effective measures for enhancing infiltration, reducing runoff, preventing erosion, controlling nonpoint source of pollutants, and eventually protecting soil and water environment. Potential benefits of PAM treatment in agricultural soil and water environments have been revealed by many research and they include low cost, easy and quick application, and suitability for use with other Best Management Practices (BMPs) for NPS control. This study reviews the various applications of PAM and discusses its further potentials in agricultural environment.

• 한국농공학회:학술대회논문집
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• 한국농공학회 2005년도 학술발표논문집
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• pp.113-116
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• 2005

Recently the existing environment-friendly method is used in irrigation and drainage canal. So, it is necessary that environment-friendly canal method which is stable and quality. Environment-friendly manufactures which were reflected demand characteristic of drainage and irrigation canal were developed. This study is focused on quality, stability for agricultural district development. According to the results, this method has quaility and economical efficiency, stability and natural characteristic, and will contribute to environment-friendly canal construction.

• 한국환경농학회지
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• 제38권3호
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• pp.213-218
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• 2019

BACKGROUND: Monitoring of the dynamic changes of chemical properties in agricultural land is very important for agricultural sustainability. Chemical properties of agricultural soils in Korea have been investigated at four-year interval in the order of paddy, plastic film house, upland, and orchard soils since 1999. METHODS AND RESULTS: Total 8,160 topsoil samples were taken from the upland in 2001, 2005, 2009, 2013, and 2017, respectively. Soil chemical properties such as pH, electrical conductivity (EC), organic matter (OM), available phosphate (Avail. $P_2O_5$), and exchangeable (Exch.) cations (K, Ca, and Mg) were analyzed. Soil pH and Exch. Ca contents have increased since 2001. Average concentration of Avail. $P_2O_5$ increased from $547mg\;kg^{-1}$ in 2001 to $657mg\;kg^{-1}$ in 2017. Average concentration of Exch. Ca in 2017 was higher than the upper limit of its optimal range for upland cultivation. Excess and deficiency of chemical properties of upland soils comply with soil analysis and fertilizer prescription. CONCLUSION: We concluded that excessive nutrient in upland needed to be properly managed with soil test.

• 농약과학회지
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• 제14권2호
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• pp.86-94
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• 2010

본 연구는 2006년 3월부터 2008년 12월까지 부산지역 농산물도매시장으로 유입되는 경매 전 농산물 7,237건에 대해 다종농약다성분법으로 잔류농약검사를 실시하였다. 이 중 1,164건 (16.1%)에서 농약이 검출되었으며, 231건 (3.2%)은 잔류허용기준을 초과하였다. 기준초과된 농산물은 대부분 해당 농산물에 대한 잔류허용기준이 정해져 있지 않아서 잠정기준을 적용받는 경우로서 기준초과된 231건 중 181건으로 78.4%가 이에 해당한다. 농약성분별로 endosulfan, procymidone, chlorothalonil, ethoprophos, chlorpyrifos, diethofencarb, kresoxim-methyl, EPN 순으로 잔류허용기준이 초과되었다. 잔류농약이 검출된 1,164건의 농산물 중 211건에서 두 종류 이상 농약이 검출되었고 이 중 37.9%에 해당되는 80건이 기준초과되었다. 본 연구에서 농약 잔류허용기준을 초과하는 요인은 해당 농산물에 대한 미적용 농약의 사용, 두 종류 이상의 농약사용으로 나타났다.

• 한국환경농학회지
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• 제28권1호
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• pp.1-8
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• 2009

도시화로 인하여 농경지가 기속적으로 줄어듦에 따라 농업의 환경적 공익기능도 축소되고 있다. 따라서 농업의 공익기능 관련 실증 분석사례를 제공함으로써 식량안보 및 공업의 공익기능을 유지하고 농지보전 정책 논리를 제공하고자, 용인시를 대상으로 토지이용도를 이용한 연차별 농경치 이용면적과 변화를 분석하고 지표면 온도 추정을 통하여 환경보전 기능을 평가하였다. 최근 10년간 도시화가 현저히 진행된 용인시를 대상으로 세부정밀토양도에 기반한 1999년 토지이용 현황과 위성영상에서 추출한 농경지 지도와 지적도에 기반한 2006년의 농경지를 중심으로 한 토지이용 현황을 비교해 보면 논, 밭, 과수원 등 농경지와 산림의 분포가 현저히 감소한 반면, 주거 도심지의 면적이 크게 확대된 것으로 나타났다. 특히 처인구에서 농경지 감소와 도심지 확장 현상이 눈에 띄게 나타났다. 토지이용 면적 및 지목별 변화를 보면 1999년 용인시 토지이용 면적은 산림 > 논 > 밭 > 주거 도심지 순으로 나타났으나 2006년의 토지이용 면적은 산림 > 주거 도심지 > 논 > 밭 순으로 바뀌었다. 논과 밭의 면적은 1999년에 비해 2006년에 각각 34%와 41%, 감소하였고, 주거 도심지 면적은 245% 증가하였다. 논이 주거 도심지로 전용된 면적이 1,751.1 ha이며, 밭이 주거.도심지로 전용된 면적이 1,242.1 ha로 나타나 전용된 면적의 가장 많은 부분을 차지하는 것으로 나타났다. 용인시의 농경지 면적 변화에 따른 농업의 환경적 기능변화에 대하여 계량화한 결과는 논 면적이 1999년에 8,063.3 ha에서 2006년에 5,309.3 ha로 감소한 결과, 농경지의 환경적 공익기능이 34% 감소한 것으로 나타났다. 마찬가지로 밭 면적이 1999년에 3,572.1 ha에서 2006년 2,112.5 ha로 줄어듦에 따라 환경적 공익기능이 41% 감소된 것으로 나타났나. Landsat TM 열상의 열 적외광을 이용하여 용인시의 두시기별 지표면 온도 분포를 비교 분석하였다. 1994년 9월에는 $20^{\circ}C$ 이하가 대부분이었으나, 농경지 및 산림 감소와 도시 확장 이후인 2006년 9월에는 $25^{\circ}C$ 이상 되는 지역의 면적이 현저히 넓게 분포하는 것으로 변화하였다. 시기별 토지이용별 지표면 온도분포 비교를 하였을 때 1994년 9월 지표면 온도 영상에서 $25^{\circ}C$ 이상인 지역은 전체 면적의 0.3%로 나타났고, 2006년 9월은 11.2%로 넓게 분포하는 것으로 나타났다. 2006년 9월에 지표면 온도가 $25^{\circ}C$ 이상 되는 지역에 분포하는 주거.도심지의 면적이 37.7%로 가장 높게 나타났으며, 논과 산림의 분포면적 비율이 각각 5.6%와 4%로 나타났다. 위의 결과로 여름철 고온기에 논과 산림이 주변의 지표면 온도를 낮춰주는 기후순화 기능이 크다는 것으로 추정할 수 있었다.

• The Plant Pathology Journal
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• 제22권2호
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• pp.174-178
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• 2006

Monacroporium ullum sp. nov. the captures nematodes on adhesive spherical knobs was isolated from soil around Codonopsis lanceolata at Ulleung island, Korea. The spindle-shaped conidia sized $17-44{\mu}m\;(26.5{\mu}m)\;long,\;7-10{\mu}m\;(8.1{\mu}m)$ wide, containing from 1 to 4 cross-walls but most often divided by 2 septa (47%). Resting bodies sized $57{\times}30{\mu}m$. Arthrobotrys amerospora has almost spherical non-septate conidia with a small truncate protuberance at the base and sized $20-27{\mu}m\;(23.3){\mu}m$ long and $11-17{\mu}m\;(14.1){\mu}m$ wide. Conidiophores are somewhat longer $362.8{\mu}m\;(311-418{\mu}m)$ than its original description ($75-250{\mu}m$).