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http://dx.doi.org/10.5423/PPJ.OA.08.2016.0177

Characterization of the rcsA Gene from Pantoea sp. Strain PPE7 and Its Influence on Extracellular Polysaccharide Production and Virulence on Pleurotus eryngii  

Kim, Min Keun (Environment-Friendly Research Division, Gyeongsangnam-do Agricultural Research & Extension Services)
Lee, Sun Mi (Environment-Friendly Research Division, Gyeongsangnam-do Agricultural Research & Extension Services)
Seuk, Su Won (Environment-Friendly Research Division, Gyeongsangnam-do Agricultural Research & Extension Services)
Ryu, Jae San (Environment-Friendly Research Division, Gyeongsangnam-do Agricultural Research & Extension Services)
Kim, Hee Dae (Environment-Friendly Research Division, Gyeongsangnam-do Agricultural Research & Extension Services)
Kwon, Jin Hyeuk (Environment-Friendly Research Division, Gyeongsangnam-do Agricultural Research & Extension Services)
Choi, Yong Jo (Environment-Friendly Research Division, Gyeongsangnam-do Agricultural Research & Extension Services)
Yun, Han Dae (Department of Applied Life Chemistry, College of Agriculture and Life Science, Gyeongsang National University)
Publication Information
The Plant Pathology Journal / v.33, no.3, 2017 , pp. 276-287 More about this Journal
Abstract
RcsA is a positive activator of extracellular polysaccharide (EPS) synthesis in the Enterobacteriaceae. The rcsA gene of the soft rot pathogen Pantoea sp. strain PPE7 in Pleurotus eryngii was cloned by PCR amplification, and its role in EPS synthesis and virulence was investigated. The RcsA protein contains 3 highly conserved domains, and the C-terminal end of the open reading frame shared significant amino acid homology to the helix-turn-helix DNA binding motif of bacterial activator proteins. The inactivation of rcsA by insertional mutagenesis created mutants that had decreased production of EPS compared to the wild-type strain and abolished the virulence of Pantoea sp. strain PPE7 in P. eryngii. The Pantoea sp. strain PPE7 rcsA gene was shown to strongly affect the formation of the disease symptoms of a mushroom pathogen and to act as the virulence factor to cause soft rot disease in P. eryngii.
Keywords
bacterial pathogen; king oyster mushroom; Pleurotus eryngii; rcsA gene; soft rot disease;
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1 Jayaratne, P., Keenleyside, W. J., MacLachlan, P. R., Dodgson, C. and Whitfield, C. 1993. Characterization of rcsB and rcsC from Escherichia coli O9:K30:H12 and examination of the role of the rcs regulatory system in expression of group I capsular polysaccharides. J. Bacteriol. 175:5384-5394.   DOI
2 Kim, M. K., Ryu, J. S., Lee, Y. H. and Yun, H. D. 2007. First report of Pantoea sp. induced soft rot disease of Pleurotus eryngii in Korea. Plant Dis. 91:109.
3 Leigh, J. A. and Coplin, D. L. 1992. Exopolysaccharides in plant-bacterial interactions. Annu. Rev. Microbiol. 46:307-346.   DOI
4 Matthysse, A. G. 1983. Role of bacterial cellulose fibrils in Agrobacterium tumefaciens infection. J. Bacteriol. 154:906-915.
5 Mergaert, J., Verdonck, L. and Kersters, K. 1993. Transfer of Erwinia ananas (synonym, Erwinia uredovora) and Erwinia stewartii to the genus Pantoea emend. as Pantoea ananas (Serrano 1928) comb. nov. and Pantoea stewartii (Smith 1898) comb. nov., respectively, and description of Pantoea stewartii subsp. indologenes subsp. nov. Int. J. Syst. Bacteriol. 43:162-173.   DOI
6 Ministry for Food, Agriculture, Forestry and Fisheries. 2015. An actual output for special crop. Ministry for Food, Agriculture, Forestry and Fisheries, Sejong, Korea. pp. 60-63.
7 Ohga, S. and Royse, D. J. 2004. Cultivation of Pleurotus eryngii on umbrella plant (Cyperus alternifolius) substrate. J. Wood Sci. 50:466-469.   DOI
8 Okamoto, H., Sato, M. and Isaka, M. 1999. Bacterial soft rot of winter mushroom and oyster mushroom caused by Erwinia carotovora subsp. carotovora. Ann. Phytopathol. Soc. Jpn. 65:460-464.   DOI
9 Pabo, C. O. and Sauer, R. T. 1984. Protein-DNA recognition. Annu. Rev. Biochem. 53:293-321.   DOI
10 Paine, S. G. 1919. Studies in bacteriosis II. A brown blotch disease of cultivated mushrooms. Ann. Appl. Biol. 5:206-219.   DOI
11 Bernhard, F., Poetter, K., Geider, K. and Coplin, D. L. 1990. The rcsA gene rom Erwinia amylovora: identification, nucleotide sequence analysis, and regulation of exopolysaccharide biosynthesis. Mol. Plant-Microbe Interact. 3:429-437.   DOI
12 Allen, P., Hart, C. A. and Saunders, J. R. 1987. Isolation from Klebsiella and characterization of two rcs genes that activate colanic acid capsular biosynthesis in Escherichia coli. J. Gen. Microbiol. 133:331-340.
13 Ausubel, F. M., Brent, R., Kingston, R. E., Morre, D. D., Seidman, J. G., Smith, J. A. and Struhl, K. 1999. Short protocols in molecular biology. 4th ed. John Wiley & Sons, New York, NY, USA.
14 Azad, H. R., Holmes, G. J. and Coolsey, D. A. 2000. A new leaf blotch disease of sudangrass caused by Pantoea ananas and Pantoea stewartii. Plant Dis. 94:973-979.
15 Bennett, R. A. and Billing, E. 1980. Origin of the polysaccharide ooze from plants infected with Erwinia amylovora. J. Gen. Microbiol. 116:341-349.
16 Bereswill, S. and Geider, K. 1997. Characterization of the rcsB gene from Erwinia amylovora and its influence on exoploysaccharide synthesis and virulence of the fire blight pathogen. J. Bacteriol. 179:1354-1361.   DOI
17 Braun, E. J. 1982. Ultrastructural investigation of resistant and susceptible maize inbreds infected with Erwinia stewartii. Phytopathology 72:159-166.   DOI
18 Brill, J. A., Quinlan-Walshe, C. and Gottesman, S. 1988. Finestructure mapping and identification of two regulators of capsule synthesis in Escherichia coli K-12. J. Bacteriol. 170:2599-2611.   DOI
19 Bruton, B. D., Wells, J. M., Lester, G. E. and Patterson, C. L. 1991. Pathogenicity and characterization of Erwinia ananas causing a postharvest disease of cantaloup fruit. Plant Dis. 75:180-183.   DOI
20 Ceponis, M. J., Wells, J. M. and Cappellini, R. A. 1985. Bacterial brown spot of honeydew melons. Hortic. Sci. 20:302-303.
21 Chatterjee, A., Chun, W. and Chatterjee, A. K. 1990. Isolation and characterization of an rcsA-like gene of Erwinia amylovora that activates extracellular polysaccharide production in Erwinia species, Escherichia coli, and Salmonella tyohimurium. Mol. Plant-Microbe Interact. 3:144-148.   DOI
22 Sutherland, I. W. 1985. Biosynthesis and composition of gramnegative bacterial extracellular and wall polysaccharides. Annu. Rev. Microbiol. 39:243-270.   DOI
23 Poetter, K. and Coplin, D. L. 1991. Structural and functional analysis of the rcsA gene from Erwinia stewartii. Mol. Gen. Genet. 229:155-160.
24 Serrano, F. B. 1928. Bacterial fruitlet brown-rot of pineapple in the Philippines. Philippines J. Sci. 36:271-305.
25 Smit, G., Kijne, J. W. and Lugtenberg, B. J. 1987. Involvement of both cellulose fibrils and a $Ca^{2+}$-dependent adhesin in the attachment of Rhizobium leguminosarum to pea root hair tips. J. Bacteriol. 169:4294-4301.   DOI
26 Stout, V. and Gottesman, S. 1990. RcsB and RcsC: a two-component regulator of capsule synthesis in Escherichia coli. J. Bacteriol. 172:659-669.   DOI
27 Stout, V., Torres-Cabassa, A., Maurizi, M. R., Gutnick, D. and Gottesman, S. 1991. RcsA, an unstable positive regulator of capsular polysaccharide synthesis. J. Bacteriol. 173:1738-1747.   DOI
28 Tolaas, A. G. 1915. A bacterial disease of cultivated mushroom. Phytopathology 5:51-54.
29 Torres-Cabassa, A., Gottesman, S., Frederick, R. D., Dolph, P. J. and Coplin, D. L. 1987. Control of extracellular polysaccharide synthesis in Erwinia stewartii and Escherichia coli K-12: a common regulatory function. J. Bacteriol. 169:4525-4531.   DOI
30 Wells, J. M., Sapers, G. M., Fett, W. F., Butterfield, J. E., Jones, J. B., Bouzar, H. and Miller, F. C. 1996. Postharvest discoloration of the cultivated mushroom Agaricus bisporus caused by Pseudomonas tolaasii, P. 'reactans', and P. 'gingeri'. Phytopathology 86:1098-1104.   DOI
31 Wong, W. C., Fletcher, J. T., Unsworth, B. A. and Preece, T. F. 1982. A note on ginger blotch, a new bacterial disease of the cultivated mushroom, Agaricus bisporus. J. Appl. Bacteriol. 52:43-48.   DOI
32 Dolph, P. J., Majerczak, D. R. and Coplin, D. L. 1988. Characterization of a gene cluster for exopolysaccharide biosynthesis and virulence in Erwinia stewartii. J. Bacteriol. 170:865-871.   DOI
33 Coleman, M., Pearce, R., Hitchin, E., Busfield, F., Mansfield, J. W. and Roberts, I. S. 1990. Molecular cloning, expression and nucleotide sequence of the rcsA gene of Erwinia amylovora, encoding a positive regulator of capsule expression: evidence for a family of related capsule activator proteins. J. Gen. Microbiol. 136:1799-1806.   DOI
34 Coplin, D. L., Frederick, R. D., Majerczak, D. R. and Haas, E. S. 1986. Molecular cloning of virulence genes from Erwinia stewartii. J. Bacteriol. 168:619-623.   DOI
35 Coutinho, T. A., Preisig, O., Mergaert, J., Cnockert, M. C., Riedel, K. H., Swings, J. and Wingfield, M. J. 2002. Bacterial blight and dieback of Eucalyptus species, hybrids, and clones in South Africa. Plant Dis. 86:20-25.   DOI
36 Fett, W. F., Osman, S. F. and Dunn, M. F. 1989. Characterization of exopolysaccharides produced by plant-associated fluorescent pseudomonads. Appl. Environ. Microbiol. 55:579-583.
37 Flippi, C., Bagnoli, G., Bedini, S., Agnoluci, M. and Nuti, M. P. 2002. Ulteriori studi sull, eziologico della batteriosi" del cardoneelo. Agricolltura Ricerca 188:53-58 (in Italian).
38 Fyfe, J. A. and Govan, J. R. 1980. Alginate synthesis in mucoid Pseudomonas aeruginosa: a chromosomal locus involved in control. J. Gen. Microbiol. 119:443-450.
39 Gitaitis, R. D. and Gay, J. D. 1997. First report of a leaf blight, seed stalk rot, and bulb decay of onion by Pantoea ananas in Georgia. Plant Dis. 81:1096.
40 Gavini, F., Mergaer, J., Behi, A., Mielcarek, C., Izard, D., Kersters, K. and De Ley, J. 1989. Transfer of Enterobacter agglomerans (Beijerinck 1888) Ewing and Fife 1972 to Pantoea gen. nov. as Pantoea agglomerans comb. nov. and description of Pantoea dispersa sp. nov. Int. J. Syst. Bacteriol. 39:337-345.   DOI
41 Gottesman, S. 1995. Regulation of capsule synthesis: modification of the two-component paradigm by an accessory unstable regulator. In: Two-component signal transduction, eds. by J. A. Hoch and T. J. Sihavy, pp. 253-262. ASM Press, Washington, DC, USA.
42 Gottesman, S., Trisler, P. and Torres-Cabassa, A. 1985. Regulation of capsular polysaccharide synthesis in Escherichia coli K-12: characterization of three regulatory genes. J. Bacteriol. 162:1111-1119.
43 Han, H. S., Jhune, C. S., Cheong, J. C., Oh, J. A., Kong, W. S., Cha, J. S. and Lee, C. J. 2012. Occurrence of black rot of cultivated mushrooms (Flammulina velutipes) caused by Pseudomonas tolaasii in Korea. Eur. J. Plant Pathol. 133:527-535.   DOI
44 Hisamatsu, M., Yamada, T., Higashiura, T. and Ikeda, M. 1987. The production of acidic, O-acetylated cyclosophorans [cyclid(1$\rightarrow$2)-${\beta}$-D-glucans] by Agrobacterium and Rhizobium species. Carbohydr. Res. 163:115-122.   DOI
45 Gea, F. J., Tello, J. C. and Navarro, M. J. 2003. Occurrence of Verticillium fungicola var. fungicola on Agaricus bitorquis mushroom crops Spain. J. Phytopathol. 151:98-100.   DOI
46 Hoang, T. T., Karkhoff-Schweizer, R. R., Kutchma, A. J. and Schweizer, H. P. 1998. A broad-host-range Flp-FRT recombination system for site-specific excision of chromosomallylocated DNA sequences: application for isolation of unmarked Pseudomonas aeruginosa mutants. Gene 212:77-86.   DOI
47 Inglis, P. W., Burden, J. L. and Peberdy, J. F. 1996. Evidence for the association of the enteric bacterium Ewingella americana with internal stipe necrosis of Agricus bisporus. Microbiology 142:3253-3260.   DOI