• Journal of Environmental Science International
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• v.28 no.11
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• pp.1027-1032
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• 2019

This study aimed to analyze the prioritized area for the functional change of agricultural reservoir according to the rapid urbanization and social changes through the mapping method. Changwon-si, Gimhae-si, and Jinju-si in Gyeongnam province were selected as the study area, considering the results of land-use and cluster analysis. As the planning unit of management area, watershed was used and land coverage map from 1975 to 2015 were analyzed for changes of land use. The reduction ratio (%) of farmland was calculated for identifying the changes in 2013 compared to 1975. As a result, the reduction ratio was 11.9% for Changwon-si, 12.2% for Gimhae-si and 9.3% for Jinju-si, and the number of watershed having functionally reduced potential-area was relatively high in the proportion to the city size.

• Journal of Digital Convergence
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• v.18 no.10
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• pp.181-194
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• 2020

Due to the changes in the distribution market, issues related to storage and distribution of agricultural, aquatic and livestock products, and storage and transportation of processed and fresh food are rapidly emerging, and as a result, Cold Chain is naturally receiving attention as one of the logistics services. The purpose of this study is to evaluate the competitiveness of location in the construction of a cold chain cluster centered on the metropolitan area, which has attracted attention in relation to the distribution of cold chains, such as recently refrigerated frozen foods. To this end, this study evaluated the competitiveness of cold chain cluster candidates in the metropolitan area by utilizing the CFPR (Consistent Fuzzy Preference Relations) method that can efficiently extract and quantify expert knowledge. As a result, the location competitiveness was found to be superior to Incheon New Port's hinterland, Gyeonggi South Area (Yongin), Gyeonggi West Area (Gimpo Logistics Complex), and Pyeongtaek Oseong Logistics Complex. In particular, this study extracted the knowledge of refrigerated and refrigerated logistics warehouse operation experts, and conducted detailed competitiveness assessments for cold chain cluster candidates in the metropolitan area, and suggested the optimal cluster candidates. In the future research, it is necessary to classify the questionnaire into the owner, large business group, and public business group, etc., who have the right to purchase and build to secure ownership of the fresh food distribution center.

• 한국균학회소식:학술대회논문집
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• 2006.10a
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• pp.32-44
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• 2006

To investigate non-aflatoxin-production of A. oryzae at the molecular level, an aflatoxin biosynthesis gene homolog cluster of RIB 40 was analyzed. Although most genes in the corresponding cluster exhibited from 97 to 99 % similarity to those of Aspergillus flavus, three genes shared 93 % similarity or less. In addition, although slight expression of aflR, positive transcriptional regulator gene, was detected in some A. oryzae strains having seven aflatoxin biosynthesis homologous genes, other genes related to aflatoxin production were not detected. RIB strains were mainly divided into group 1, having seven aflatoxin biosynthesis homologous genes (aflT, nor-i, aflR, norA, avnA, verB, and vbs), and group 2, having three homologous (avnA, verB, and vbs). Partial aflatoxin homologous gene cluster of RIB62 from group 2 was sequenced and compared with that of RIB40 from group 1. RIB62 showed a large deletion upstream of ver-1 with more than half of the aflatoxin homologous gene cluster missing including aflR, a positive transcriptional regulatory gene. Adjacent to the deletion of the aflatoxin homologous gene cluster, RIB62 has a unique sequence of about 8kb and a telomere. Southern analysis of A. oryzae RIB strains with four kinds of probe derived from the unique sequence of RIB62 showed that all group 2 strains have identical hybridizing signals. Polymerase chain reaction with specific primer set designed to amplify the junction between ver-1 and the unique sequence of RIB62 resulted in the same size of DNA fragment only from group 2 strains. Based on these results, we developed a useful genetic tool that distinguishes A. oryzae group 2 strains from the other groups' strains and propose that it might have differentiated from the ancestral strains due to chromosomal breakage.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.63 no.1
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• pp.1-7
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• 2018

Information on the patterns of genetic diversity and population structure is essential for the rational use and efficient management of germplasms; accurate information aids in monitoring germplasms, and can also be used to predict potential genetic gains. In this study, we assessed genetic diversity, focusing on Korean rice accessions for theand their sustainable conserved diversity. Using DNA profiling with 12 simple sequence repeat (SSR) markers, we detected a total of 333 alleles among 2,016 accessions. The number of alleles ranged from 21 to 53, with an average of 27.8. Average polymorphism information content was 0.797, with the lowest being 0.667 and the highest 0.940. CA cluster analysis and the model-based population structure revealed two main groups that could be subdivided into five subgroups. Analysis of the molecular variance study based on the SSR profile data showed 5% variance among the profiles, whereas we recorded 93% variance among individuals and 2% variance within individuals. Specifically, the utilized diversity for of the breeding program is restricted in that cultivars were located in limited clades. These results revealed that preserving the diversity of Korean landraces could be useful sources for breeding new rice cultivars, and cwould be the basis for the sustainable conservation and utilization of a Korean rice germplasm.

• Food Science and Biotechnology
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• v.18 no.4
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• pp.1001-1012
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• 2009

A total of 49 phenolic compounds were identified from the aged red wines made from Cabernet Gernischt (Vitis vinifera L. cv.) grapes, a Chinese characteristic variety, including 13 anthocyanins, 4 pryanocyanins, 4 flavan-3-ol monomers, 6 flavan-3-ol polymers, 7 flavonols, 6 hydroxybenzoic acids, 5 hydroxycinnamic acids, 3 stilbenes, and 1 polymeric pigment. Evolution of these compounds was investigated in wines aged 1 to 13 months. Variance analysis showed that the levels of most phenolics existed significant difference in between wines aged 3 and 9 months. Cluster analysis indicated that 2 groups could be distinguished, one corresponding to wines aged 1 to 3 months and the other to wines aged 4 to 13 months. It was thus suggested that there were 2 key-stages for the development of fine wine quality, at the aged 3 and 9 months, respectively. This work would provide some helpful information for quality control in wine production.

• Journal of Microbiology and Biotechnology
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• v.19 no.9
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• pp.851-857
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• 2009

We developed and characterized 36 polymorphic microsatellite markers for the oyster mushroom (Pleurotus ostreatus). In total, 169 alleles were identified with an average of 4.7 alleles per locus. Values for observed ($H_o$) and expected ($H_E$) heterozygosities ranged from 0.027 to 0.946 and from 0.027 to 0.810, respectively. Nineteen loci deviated from Hardy-Weinberg equilibrium. Significant (P<0.05) excess heterozygosity was observed at nine loci. Linkage disequilibrium (LD) was significant (P<0.05) between pairs of locus alleles. Cluster analysis revealed that five species of genus Pleurotus made a distinct group, and the individual cultivars were grouped into major five groups from G-1 to G-5. The diverse cultivars of P. ostreatus were discriminated and the other four species revealed a different section in the UPGMA tree. These microsatellite markers proved to be very useful tools for genetic studies, including assessment of the diversity and population structure of P. ostreatus.

• The Korean Journal of Pesticide Science
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• v.14 no.3
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• pp.266-271
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• 2010

Six insecticides, monocrotophos (24%, Lq), milbemectin (1%, Ec), tebufenpyrad (10%, Ec), propargite (30%, Wp), dicofol (42%, Ec), and fenpropathrin (5%, Ec) were studied for their $LC_{50}s$ to local two-spotted spider mite (TSSM) strains collected at apple orchards in Chungju, Kunwi and Sobo in Korea. Monocrotophos and fenpropathrin were not effective due to resistance development, but milbemectin, tebufenpyrad, dicofol and propargite were effective to TSSM. $LC_{50}$ values to TSSM strains showed the same distribution pattern among apple orchards. However, TSSMs from different apple orchard were clustered into different groups.

• International Journal of Industrial Entomology and Biomaterials
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• v.17 no.2
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• pp.229-234
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• 2008

We used serial analysis of gene expression (SAGE) approach to derive a profile of expressed genes of the posterior silk glands (PSG) and to create a reference for understanding gene cluster related to the mechanism of silk protein synthesis in the silkworm, Bombyx mori. We constructed a 3' SAGE library from the PSG of the fifth instar larvae of the silkworm. In total we obtained 2,406 SAGE tags, of which 682 were unique tags. Sorted by tag count number, 27 (4%) unique tags were significantly more abundant genes (ten or more times), whereas 445 (65%) unique tags were detected as single copies. The annotation of 682 unique SAGE tags revealed that 462 (68%) of the SAGE tag sequences represented known genes, whereas 220 (32%) of the tag sequences had no matches in SAGE map and silkworm EST databases. Of the 682 SAGE tags, the most abundant tag sequences were that of the fibroin light chain gene and the silk protein P25. In addition, we compared two relative abundance results of the SAGE and the EST approaches to verify whether their transcript quantitative aspects are significant or not. The comparative results of relative abundances of the fibroin H-, L- chain and P25 glycoprotein genes indicated that the quantitative approach based on SAGE tags is effective for quantitative cataloging and comparison of expressed genes in same organs. The SAGE tag information reported in this study would be useful for researchers in the field to analyze genes associated with silk processing mechanisms of insects.

• Proceedings of the Korean Society of Plant Pathology Conference
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• 2003.10a
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• pp.141.1-141
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• 2003

Forty six isolates of bean common mosaic virus (BCMV) collected from azuki bean, mungbean, kidney bean, cowpea, broad bean and peanut were classified into three groups based on biological, serological, cytopathological, and molecular characteristics. Group I induced vein-banding symptoms in cowpea which was similar to those produced by the BCMV-cowpea strain. Group II caused mosaic symptoms in azuki bean but not in peanut and tobacco. Since this character was different from that of previously described BCMV strain, group II may not belong to BCMV GroupIII induced vein-clearing symptoms in azuki bean, kidney bean and peanut, which are typical symptoms for BCMV-peanut stripe virus strain. Virus inclusion patterns of BCMV groups were similar to those of Potyvirus subdivision III with the scroll, pinwheel and long laminated inclusions. However, the inclusions of laminated aggregates were never observed in mungbean isolates. Multiple alignment as well as cluster dendrograms of 3'noncoding region (3'-NCR) and a part of coat protein gene (CP) suggested that group I belongs to the BCMV-cowpea strain, group II to the BCMV-azuki bean strain, and group III to the BCMV-peanut stripe virus strain. Since molecular phylogenesis of BCMV based on nucleotides of 3'-NCR and coat protein differed from the grouping based on virulence differentiation, and BCMV groups are more closely related to each other with the same host origin, other characteristics of those strains are under investigation.

• Korean Journal of Agricultural Science
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• v.39 no.1
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• pp.1-8
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• 2012

Rapid loss of grape berry quality after harvest occurs due to poor postharvest management. Understanding physiological properties between cultivars is required to develop practical technologies to control fast quality deterioration of tables grapes. Physiological characteristics of whole cluster, rachis and berries were examined to find their effects on postharvest behaviour of table grapes. 'Tamnara' showed high respiration rate and browning of rachis compared to 'Campbell Early'. Weight loss of rachis of 'Cheongsoo' was highest of three cultivars. Berry decay in 'Cheongsoo' begins at the connection portion between peduncle and berry, and berry decay of 'Cheongsoo' was the most severe among three cultivars. Precooling at $0^{\circ}C$ showed better results than cooling at $10^{\circ}C$ regardless of cultivars but cooling effect decreased when simulated marketing period increased up to 10 days. Plastic film wrapping was effective on reducing decay in general but not at higher marketing temperature. For successful commercialization of newly developed grapes, the inhibition technology of rachis browning in 'Tamnara' is required and decay control in 'Cheongsoo' through establishment of cold chain system is recommended.