• Korean Journal of Medicinal Crop Science
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• v.4 no.3
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• pp.224-230
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• 1996

Randomly amplified polymorphic DNA(RAPD) analysis was applied to detect the molecular polymorphisms in Gentiana scabra var. buergeri. A high level of molecular variability was found among wild plants and cultivars. In genetic analysis, eight of twenty primers were selected and 54 amplification products ranged 2. 2 to 0.2 kb were compared. Twenty - nine amplified products showed polymorphic, while five were monomorphic. Twenty of line specific bands were found. In genetic similarity and cluster analysis using PCR products, three wild plants collected from Naejangsan, Daedunsan and Keojedo and one cultivar Seochunjaerae were grouped into one cluster, while cultivar Jinbujaerae and Japanese line separated into another clusters, respectively. The identification of DNA polymorphisms by the RAPD technique will facilitate the selection of the lines from different origin.

• Asian-Australasian Journal of Animal Sciences
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• v.27 no.6
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• pp.898-906
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• 2014

The objective of this study was to investigate differences in the faecal microbial composition among Lantang, Bama, Erhualian, Meishan, Xiaomeishan, Duroc, Landrace, and Yorkshire sows and to explore the possible link of the pig breed with the gut microbial community. Among the sows, the Meishan, Landrace, Duroc, and Yorkshire sows were from the same breeding farm with the same feed. Fresh faeces were collected from three sows of each purebred breed for microbiota analysis and volatile fatty acid (VFA) determination. Denaturing gradient gel electrophoresis (DGGE) analysis revealed that samples from Bama, Erhualian, and Xiaomeishan sows, which from different farms, were generally grouped in one cluster, with similarity higher than 67.2%, and those from Duroc, Landrace, and Yorkshire sows were grouped in another cluster. Principal component analysis of the DGGE profile showed that samples from the foreign breeds and the samples from the Chinese indigenous breeds were scattered in two different groups, irrespective of the farm origin. Faecal VFA concentrations were significantly affected by the pig breed. The proportion of acetate was higher in the Bama sows than in the other breeds. The real-time PCR analysis showed that 16S rRNA gene copies of total bacteria, Firmicutes and Bacteroidetes were significantly higher in the Bama sows compared to Xiaomeishan and Duroc sows. Both Meishan and Erhualian sows had higher numbers of total bacteria, Firmicutes, Bacteroidetes and sulphate-reducing bacteria as compared to Duroc sows. The results suggest that the pig breed affects the composition of gut microbiota. The microbial composition is different with different breeds, especially between overseas breeds (lean type) and Chinese breeds (relatively obese type).

• Korean Journal of Agricultural Science
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• v.49 no.4
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• pp.1015-1031
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• 2022

The objectives of this study were to classify the types of elderly households and to compare the characteristics of their dietary lifestyle. Panel data surveyed by Korea Rural Economic Institute (KREI) for Food Purchase Attitudes over three years (2019 - 2021) were utilized for the analysis. Through a factor analysis, five common factors were extracted out of 19 basic variables related to dietary style, which indicate two kinds of consumer competency index (safe diet, traditional diet) and three kinds of purchase frequency (healthy food, meat & fish, fresh seafood). Applying the cluster analysis method, by using socioeconomic variables along the five common factors, elderly households aged 60 or older were grouped into four types. As a result, Type 1 elderly households accounted for 50.8%, Type 2 for 16.2%, Type 3 for 27.8%, and Type 4 for 5.2% out of all 870 elderly households. Type 1 is characterized as a low-income vulnerable class with a poor diet, Type 2 as a middle-income class with a healthy food-oriented diet, whereas Type 3 was classified as a middle-income class with a meat-oriented diet, and Type 4 as a high-income class with diverse dietary culture. It is necessary to expand the agri-food voucher pilot project to the entire country and also increase the monthly subsidy for the Type 1 elderly households. Implementing community kitchen projects for elderly single-person households, promoting senior internships by providing incentives to companies that employ retirees, the provision of education by local governments on a safe and balanced diet for Types 2 and 3, and the promotion of an elderly-friendly social environment are also recommended.

• Korean Journal of Plant Resources
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• v.28 no.2
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• pp.290-295
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• 2015

The experiment were carried out in 7-year-old ‘Campbell Early’ grape to increase work efficiency by fruit cluster thinning methods (Pedicel and berry thinning). Pedicel thinning, such as labor-saving cluster thinning of grape, was 6.7 fold higher than the berry thinning for work efficiency. The fruit cluster weight and number of berry were lower in the fruit cluster thinning fruits than in the none-fruit cluster thinning, however, the soluble solid content (SSC) was high and titratable acidity (TA) was low in the fruit cluster thinning fruits than in the none-fruit cluster thinning fruits. Therefore, the bruising rate of berries was decreased in the fruit cluster thinning fruits. Quality uniformity by fruit cluster thinning was proper in fruit cluster weight of 350~450 g when SSC and TA in part of lower, middle and upper of cluster was considered.

• The Korean Journal of Mycology
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• v.27 no.6 s.93
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• pp.406-411
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• 1999

The phylogenetic relationships of black-spored basidioid taxa (mainly coprinoid taxa) and an agaric-like gastroid taxon were studied. The sequences of internal transcribed spacer regions (ITS) partially including 17S, 25S and 5.8S from 14 species (Coprinus comatus, C. atramentarius, C. micaceus, C. cinereus, C. disseminatus, C. rhizophorus, C. radians, C. echinosporus, Psathyrella candolleana, Podaxis pistillaris, Conocybe lactea, Bolbitius demangei, Agaricus balzei, and Stropharia rugosoannulata) were compared. The reciprocal homologies of ITS sequences among these species were in the range of $38.7{\sim}77.2%$. Black-spored taxa were classified into four clusters. Cluster I comprised C. micaceus, C. radians, and C. disseminata. Cluster II is consisted of C. cinereus, C. echinosporus, C. rhizophorus, and C. atramentarius. On the other hand, C. comatus is in cluster III with Agaricus balzei and Podaxis pistillaris even though this species is belonging to the section Coprinus in morphological aspect. Psathyrelloid taxon is included in cluster II. The question of the origin of secotioid (agaric-like) fungi has been taken, though largely on theoritical suggestions as to whether gastroid taxa give rise to agarics with secotioid taxa as intermediates or whether secotioid taxa are evolutionary novelities arising from many distinct groups of agarics. In this relationship, it was shown that secotioid taxon evolved from within agaric species.

• Korean Journal of Soil Science and Fertilizer
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• v.40 no.4
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• pp.262-268
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• 2007

To characterize morphological classification of the basins, four major basin characteristics of the unit basins, including sinuosity, ratio of forest, ratio of flat area, and tributary existence were selected for cluster analysis. The analysis was carried out using soil map, topographic map, water course map, and basin map of the fifty unit basins in the Yeongsangang Basin. The unit basins could be categorized to five basin groups. The fitness by the Mantel test showed good fit of which r was 0.830. These grouping based on comprehensive soil and topographic characteristics provides best management practices, water quality management according to pollutants, increased water related model application and reasonable availability of water management. For agricultural management of water resources and conservation of water quality from agricultural non-point pollutants, therefore, comprehensive systematic classification of soil characteristics on unit basin might be an useful tool.

• Asian-Australasian Journal of Animal Sciences
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• v.26 no.4
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• pp.463-469
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• 2013

Cluster of differentiation 4 (CD4) is mainly expressed on $CD4^+$ T cells, which plays an important role in immune response. The aim of this study was to detect the association between polymorphisms of the CD4 gene and T lymphocyte subpopulations in pigs, and to investigate the effects of genetic variation on the CD4 gene expression level in immune tissues. Five missense mutations in the CD4 gene were identified using DNA pooling sequencing assays, and two main haplotypes (CCTCC and AGCTG) in strong linkage disequilibrium (with frequencies of 50.26% and 46.34%, respectively) were detected in the population of Large White pigs. Our results indicated that the five SNPs and the two haplotypes were significantly associated with the proportions of $CD4^-CD8^-$, $CD4^+CD8^+$, $CD4^+CD8^-$, $CD4^+$ and $CD4^+/CD8^+$ in peripheral blood (p<0.05). Gene expression analysis showed the mRNA level of the CD4 gene in thymus was significantly higher than that in lymph node and spleen (p<0.05). However, no significant difference was observed between animals with CCTCC/CCTCC genotype and animals with AGCTG/AGCTG genotype in the three immune tissues (p>0.05). These results indicate that the CD4 gene may influence T lymphocyte subpopulations and can be considered as a candidate gene affecting immunity in pigs.

• Journal of Applied Biological Chemistry
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• v.66
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• pp.484-491
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• 2023

Maize is one of the major crops consumed in worldwide, which nutrients accounts for a large amount of starch, but also functional components, and phenolic acid is known to have a high content. Maize is divided into waxy maize, sweet maize, and normal maize with its shape and use, therefore there is also a difference in nutritional composition. This study was conducted to analyze the content of functional components according to the type of maize and to produce natural variation data in consideration of environmental factors. 3 shapes of maize (waxy maize, sweet maize, and normal maize) samples cultivated in 3 regions (Suwon, Daegu, and Hongcheon) were analyzed using HPLC and GC-TOF-MS. Comparing with type through ANOVA, multivariate statistical analysis, Pearson correlation analysis, 28 components, including carotenoids and tocopherols, showed significant differences among a total of 32 components (p <0.05), 15 of them showed very significant differences (p <0.001). When comparing with regions, 15 components showed significant differences and only vanillate, syringate, C23-ol of them showed most significant differences (p <0.001). As a result of principal component analysis, cluster classification was distinguished by shape than by region, with α-carotene, cholesterol for waxy maize, vanillate and stigmasterol for sweet maize, lutein and β-carotene for normal maize had a great effect on cluster formation. It suggests that the content of functional components is more affected by genetic factors than environmental factors.

• Mycobiology
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• v.29 no.2
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• pp.85-89
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• 2001

URP primers of 20 mer derived from repetitive sequence of rice were used to assess genetic variation of oyster mushroom consisting of 10 cultivars of Pleurotus ostreatus, two cultivars of P. florida and two cultivars of P. sajor-caju which were registered in Korea. URP2F and URP38F primers produced cultivar-specific PCR polymorphic bands in the Pleurotus species. UPGMA cluster analysis using the URP-PCR data showed that 14 Pleurotus cultivars are genetically clustered into large three groups. The URP-PCR data provided important information for more efficient breeding strategies of Pleurotus cultivars.

• The Plant Pathology Journal
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• v.17 no.4
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• pp.201-204
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• 2001

Ultrastructural observation was conducted for the cells of oriental cabbage, Brassica campestris ssp. pekinensis 'Chungawang', inoculated simultaneously with Turnip mosaic virus (TuMV-ACT2-4vq) and Ribgrass mosaic virus (RMV-Ca1dn2) which were known as major destructive viruses of oriental cabbage in Korea. In cells infected with RMV alone, the virus particles were located as bundle or scattering in cytosols and vacuoles, which were typical ultrastructures of tobamovirus. Vessels of xylem were compacted with RMV particles. The cells infected only with TuMV had the cluster of virus particles scarcely and the typical potyvirus inclusions of scrolls, pinwheels, tubes and laminated aggregates in cytosols. The TuMV particles were jammed lineally between tonoplasts. In double infection, the two unrelated viruses of TuMV-ACT2-4vq and RMV-CA1dn2 were located together in a cell, and typical properties of each virus were also observed. The potyvirus inclusions and the tobamovirus particles were mixed entirely in cytoplasm. The virus particles of RMV wre presented strikingly near and in the center of potyvirus inclusions. In vascular cells, the tobamovirus particles were located abundantly than those in single infection. The potyvirus inclusions were embedded in the cluster of RMV particles in phloem parenchyma cells and the vascular elements were degenerated severely.