• 디지털융복합연구
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• 제13권3호
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• pp.215-220
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• 2015

베이비부머 세대는 현재의 저 출산 고령화 현상과 맞물려 다양한 사회문제를 낳을 것으로 예상되는데 정부의 정책들과 함께 이 세대의 삶의 패턴은 어떻게 변화되고 있으며, 시간이 지남에 따라 이 세대 삶의 웰니스 요인은 어떻게 변화하는지 보고자 하였다. 본 연구를 위한 자료는 한국복지패널에서 태어난 년도가 1955년-1963년생 가구원을 중심으로 삶의 웰니스에 영향을 미치는 요인의 변화를 확인하기 위해 2006년도부터 2011년도까지 생활수준, 건강, 수입, 직업, 여가, 가족관계, 사회적 관계, 삶의 만족과의 관계를 구조방정식모형을 통해 분석하여 살펴보았다. 연구결과, 베이비부머 세대들의 삶의 만족은 직업을 통해서 가장 크게 느끼고 있었으며, 사회적 관계 또한 직업이 가장 많은 영향을 미쳤다. 현재 수입에 대한 안정이나 만족보다는 앞으로 삶을 위한 보장을 좀 더 우선시하는 경향이 있는 것으로 나타났다. 또한 삶의 웰니스를 향상시키는 것으로 원만한 가족관계가 중요하였다.

• 한국생활과학회지
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• 제19권6호
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• pp.1103-1113
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• 2010

The purpose of this study was to examine the consumption behaviors regarding environment-friendly agricultural products by food-related lifestyles of housewives. The data were collected from 298 housewives living in Yeungnam region through a self-administered questionnaire during April, 2010. Frequencies, Cronbach's $\alpha$, factor analysis, cluster analysis, one-way analysis of variance, Duncan's multiple range test and chi-square test were conducted by SPSS Windows. The results obtained from this study were as follows. Lifestyles were categorized into five factors by factor analysis: healthy-seeking type, popularity-seeking type, convenience-seeking type, safety-seeking type and taste-seeking type. The respondents belonged to one of three groups by cluster analysis: popularity-seeking group, convenience-seeking group, and wellbeing-seeking group. The main reason given for purchasing environment-friendly agricultural products was safety. The respondents were satisfied with the safety of environment-friendly agricultural products the most. Many respondents purchased environment-friendly agricultural products at large discount markets, and received information about them from mass media. There were significant differences among three groups in the place of purchase environment-friendly agricultural products, information source for environment-friendly agricultural products, most important factor when purchasing environment-friendly agricultural products, the level of satisfaction with environment-friendly agricultural products and the purchase rate of environment-friendly agricultural products among the monthly food expenses.

• Molecules and Cells
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• 제38권2호
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• pp.163-170
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• 2015

Lipoteichoic acid (LTA) is a major component of the cell wall of Gram-positive bacteria. Its effects on living organisms are different from those of lipopolysaccharide (LPS) found in Gram-negative bacteria. LTA contributes to immune regulatory effects including anti-aging. In this study, we showed that LTA isolated from Lactobacillus plantarum (pLTA) inhibited melanogenesis in B16F10 mouse melanoma cells. pLTA reduced the cellular activity of tyrosinase and the expression of tyrosinase family members in a dose-dependent manner. The expression of microphthalmia- associated transcription factor (MITF), a key factor in the synthesis of melanin, was also decreased by pLTA. Further, we showed that pLTA activated melanogenesis signaling, such as extracellular signal-regulated kinase (ERK) and phosphatidylinositol 3-kinse (PI3K)/AKT. In addition, the expression of heterogeneous nuclear ribonucleoprotein A1 (hnRNP A1) and HuR, which are important RNA-binding proteins (RBPs), was reduced. pLTA likely degrades MITF via regulation of melanogenic signaling and RNA stability of melanogenic proteins, resulting in the reduction of melanin. Thus, our data suggest that pLTA has therapeutic potential for treating hyperpigmentation disorders and can also be used as a cosmetic whitening agent.

• 산업식품공학
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• 제13권2호
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• pp.79-84
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• 2009

딸기 분말의 대체량을 0-6%로 달리하여 쿠키를 제조한 후 물리화학적 품질특성을 측정하고 각 특성사이의 상관관계를 살펴보았다. 딸기 분말 대체량이 증가함에 따라 반죽의 pH와 쿠키의 경도는 유의적으로 감소하였다(p<0.05). 반죽의 수분함량은 딸기 분말의 대체에 따라 유의적인 차이를 나타내지는 않았지만 평균 수분함량은 증가하는 경향을 나타내었다. 딸기 분말의 대체량이 증가할수록 명도와 황색도는 유의적으로 감소한 반면 적색도는 증가하였다(p<0.05). 퍼짐성 지수 역시 유의적인 증가를 나타내었다(p<0.05). 한편 상관분석결과 딸기 분말 대체수준은 모든 물리화학적 품질 특성과 유의적인 상관관계를 나타내었다. 또한 반죽의 수분함량과 퍼짐성 지수 사이에 양의 유의적 상관관계가 관측되었다(p<0.05).

• 한국농공학회논문집
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• 제61권3호
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• pp.101-112
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• 2019

This study developed selection criteria of small-scales reservoirs, having under $300,000m^3$ storage capacity, for the Emergency Action Plan(EAP) establishment in order to reduce the disaster risks of the reservoir's failures. Those reservoirs are out of ranges of Korean EAP establishment standard, but have potential risk of disasters as they have often failed by the recent extreme rainfall events and earthquakes, causing economical and life losses. The problem of reservoir aging is also one of the reasons of them. In this study, the developed selection criteria of small reservoirs for EAP establishment are storage capacity, embankment height, reservoir age, heavy rain factor and earthquake factor. These criteria were selected based on the review of the existing EAP establishment guidelines, analysis of the past dam failure cases, and the previous related studies. The quantification of these criteria were conducted for the practical applications in the fields, and applied to 67 previous failures in order to investigate the relation of each criteria with these failures. The earthquake factor found to be the highest relations followed by heavy rain factors, combination of earthquake and heavy rain factors, and reservoir age. The classification was made as observation and review groups for EAP establishments based on overlapping numbers of each criteria. This classifications applied to 354 reservoirs designated as having the potential disaster risk by MOIS, and showed 38.4% of observation and 11.9% of review groups. Anticipatory monitoring and regular inspection should be made by professional facility managers for the observation group, and necessity of EAP establishment should be assessed for the review group based on the downstream status and financial budget.

• Biomolecules & Therapeutics
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• 제29권1호
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• pp.90-97
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• 2021

Ultraviolet B (UVB) radiation causes DNA base modifications. One of these changes leads to the generation of 8-oxoguanine (8-oxoG) due to oxidative stress. In human skin, this modification may induce sunburn, inflammation, and aging and may ultimately result in cancer. We investigated whether phloroglucinol (1,3,5-trihydroxybenzene), by enhancing the expression and activity of 8-oxoG DNA glycosylase 1 (Ogg1), had an effect on the capacity of UVB-exposed human HaCaT keratinocytes to repair oxidative DNA damage. Here, the effects of phloroglucinol were investigated using a luciferase activity assay, reverse transcription-polymerase chain reactions, western blot analysis, and a chromatin immunoprecipitation assay. Phloroglucinol restored Ogg1 activity and decreased the formation of 8-oxoG in UVB-exposed cells. Moreover, phloroglucinol increased Ogg1 transcription and protein expression, counteracting the UVB-induced reduction in Ogg1 levels. Phloroglucinol also enhanced the nuclear translocation of nuclear factor erythroid 2-related factor 2 (Nrf2) as well as Nrf2 binding to an antioxidant response element located in the Ogg1 gene promoter. UVB exposure inhibited the phosphorylation of protein kinase B (PKB or Akt) and extracellular signal-regulated kinase (Erk), two major enzymes involved in cell protection against oxidative stress, regulating the activity of Nrf2. Akt and Erk phosphorylation was restored by phloroglucinol in the UVB-exposed keratinocytes. These results indicated that phloroglucinol attenuated UVB-induced 8-oxoG formation in keratinocytes via an Akt/Erk-dependent, Nrf2/Ogg1-mediated signaling pathway.

• Journal of Microbiology and Biotechnology
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• 제30권12호
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• pp.1896-1904
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• 2020

Muscle atrophy, characterized by a reduced number and size of myofibers, occurs due to immobilization, aging, and several chronic diseases. Leonurus japonicus, belonging to the Labiatae family, is widely used as a traditional medicine in Korea, China, and Japan. Previous studies have reported that L. japonicus has various physiological activities, such as anti-bacteria, anti-cancer, and liver protection. Leonurine, which is a major bioactive in L. japonicas, is known to possess biological effects including anti-inflammation, anti-fibrosis, anti-angiogenesis, and anti-diabetes. However, the preventive effects of L. japonicas and leonurine on muscle have not been reported. The current study aimed to determine the inhibitory effects of standardized L. japonicus extract (LJE) and leonurine on muscle atrophy by clarifying their underlying molecular mechanisms in tumor necrosis factor-alpha (TNF-α)-stimulated L6 myotubes. LJE and leonurine stimulated the phosphatidylinositol 3-kinase/Akt pathway that was reduced by TNF-α treatment. LJE and leonurine not only increased the mammalian target of rapamycin pathway for protein anabolism but also decreased the mRNA expression of E3 ubiquitin ligases by blocking the translocation of Forkhead box O, which is closely linked with proteolysis. Additionally, LJE and leonurine alleviated inflammatory responses by downregulating TNF-α and interleukin-6 mRNA expression and reducing the protein expression of nuclear factor-kappa B, a major transcriptional factor of proinflammatory cytokines. Collectively, LJE and leonurine have potential as therapeutic candidates for inhibiting the development of skeletal muscle atrophy by activating the PI3K/Akt pathway and reducing inflammatory responses.

• 한국압력기기공학회 논문집
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• 제17권1호
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• pp.36-48
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• 2021

As the material aging of nuclear power plants has been progressing in domestic and overseas, crack growth becomes one of the most important issues. In this respect, the crack growth assessment has been considered an essential part of structural integrity. The crack growth assessment for nuclear power plants has been generally performed based on ASME B&PV Code, Sec. XI but the idealization of crack shape and the conservative solutions of stress intensity factor (SIF) are used. Although finite element analysis (FEA) based on iterative crack growth analysis is considered as an alternative method to simulate crack growth, there are yet no guidelines to model the crack-tip spider-web mesh for such analysis. In this study, effects of various meshing factors on FE SIF calculation are systematically examined. Based on FEA results, proper criteria for spider-web mesh in crack-tip are suggested. The validation of SIF calculation method through mapping initial stress field is investigated to consider initial residual stress on crack growth. The iterative crack-tip modeling program to simulate crack growth is developed using the proposed criteria for spider-web mesh design. The SIF results from the developed program are validated by comparing with those from technical reports of other institutes.

• 농업과학연구
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• 제48권4호
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• pp.1003-1013
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• 2021

Thioredoxin (TRX) protein is an antioxidant responsible for reducing other proteins by exchanging cysteine thiol-disulfide and is also known for its anti-allergic and anti-aging properties. On the other hand, epidermal growth factor (EGF) is an important material used in the cosmetics industry and an essential protein necessary for dermal wound healing facilitated by the proliferation and migration of keratinocytes. EGF also assists in the formation of granulation tissues and stimulates the motility of fibroblasts. Hence, genetically modified soybeans were developed to overexpress these industrially important proteins for mass production. A single-dose oral-toxicity-based study was conducted to evaluate the potential toxic effects of TRX and EGF proteins, as safety assessments are necessary for the commercial use of seed-specific protein-expressing transgenic soybeans. To achieve this rationale, TRX and EGF proteins were mass purified from recombinant E. coli. The single-dose oral-toxicity tests of the TRX and EGF proteins were carried out in six-week old male and female Institute of Cancer Research (ICR) mice. The initial evaluation of the single-dose TRF and EGF treatments was based on monitoring the toxicity signatures and mortality rates among the mice, and the resultant mortality rates did not show any specific clinical symptoms related to the proteins. Furthermore, no significant differences were observed in the weights between the treatment and control groups of male and female ICR mice. After 14 days of treatment, no differences were observed in the autopsy reports between the various treatment and control groups. These results suggest that the minimum lethal dose of TRX and EGF proteins is higher than the allowed 2,000 mg·kg^-1 limit.

• 한국식품영양과학회지
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• 제33권2호
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• pp.291-298
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• 2004

김치에는 여러 가지 항산화 물질들이 많이 함유되어 있으므로 김치의 섭취는 생체조직의 노화억제 효과가 있을 것으로 기대된다. 본 연구에서 배추김치, 갓김치, 부추김치를 동결건조하여 항산화물질의 함량을 측정한 결과 갓김치와 부추김치의 클로로필 함량이 가장 높았고 $\beta$-카로텐과 ascorbic acid 함량은 갓김치에서 가장 높았다. Hairless mouse에게 10% 동결건조 김치식이를 공급하여 20주간 사육한 후 간에서 활성산소종의 생성량을 측정한 결과 superoxide anion과hydroxyl radical 함량은 유의적인 차이가 없었고 hydrogen peroxide 함량은 갓김치군과 부추김치군에서 유의적으로 낮게 나타나 이들 김치가 활성산소를 소거하는데 효과가 있는 것으로 나타났다. 간 균질액에서 지질과산화물을 측정한 결과 대조군에 비해 김치섭취군에서 지질과산화가 낮게 나타났으며, 특히 갓김치 와 부추김 치군의 효과가 우수하였다. 피부에서 지질과산화물 함량은 김치섭취군들이 대조군보다 낮게 나타났으며, 부추김치가 유의적으로 지질과산화를 억제하였다. 김치섭취가 광산화에 미치는 영향을 규명하기 위해 간 및 피부조직에 자외선을 조사한 후 조직산화 정도를 측정한 결과 간과 피부의 지질과산화물 함량은 갓김치군과 배추김치군에서 대조군보다 낮아졌으나 부추김치군에서는 높게 나타났다. 즉, 자외선 조사 후 갓김치군과 배추김치군은 대조군보다 광산화가 억제되었으나 부추김치군에서는 산화가 촉진되었다.