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http://dx.doi.org/10.14348/molcells.2015.2263

Lipoteichoic Acid Isolated from Lactobacillus plantarum Inhibits Melanogenesis in B16F10 Mouse Melanoma Cells  

Kim, Hye Rim (School of Biotechnology and Institute of Life Science and Resources, College of Life Science, Kyung Hee University)
Kim, Hangeun (School of Biotechnology and Institute of Life Science and Resources, College of Life Science, Kyung Hee University)
Jung, Bong Jun (School of Biotechnology and Institute of Life Science and Resources, College of Life Science, Kyung Hee University)
You, Ga Eun (School of Biotechnology and Institute of Life Science and Resources, College of Life Science, Kyung Hee University)
Jang, Soojin (Institute Pasteur Korea)
Chung, Dae Kyun (School of Biotechnology and Institute of Life Science and Resources, College of Life Science, Kyung Hee University)
Publication Information
Molecules and Cells / v.38, no.2, 2015 , pp. 163-170 More about this Journal
Abstract
Lipoteichoic acid (LTA) is a major component of the cell wall of Gram-positive bacteria. Its effects on living organisms are different from those of lipopolysaccharide (LPS) found in Gram-negative bacteria. LTA contributes to immune regulatory effects including anti-aging. In this study, we showed that LTA isolated from Lactobacillus plantarum (pLTA) inhibited melanogenesis in B16F10 mouse melanoma cells. pLTA reduced the cellular activity of tyrosinase and the expression of tyrosinase family members in a dose-dependent manner. The expression of microphthalmia- associated transcription factor (MITF), a key factor in the synthesis of melanin, was also decreased by pLTA. Further, we showed that pLTA activated melanogenesis signaling, such as extracellular signal-regulated kinase (ERK) and phosphatidylinositol 3-kinse (PI3K)/AKT. In addition, the expression of heterogeneous nuclear ribonucleoprotein A1 (hnRNP A1) and HuR, which are important RNA-binding proteins (RBPs), was reduced. pLTA likely degrades MITF via regulation of melanogenic signaling and RNA stability of melanogenic proteins, resulting in the reduction of melanin. Thus, our data suggest that pLTA has therapeutic potential for treating hyperpigmentation disorders and can also be used as a cosmetic whitening agent.
Keywords
Lactobacillus plantarum; lipoteichoic acid; melanogenesis; microphthalmia-associated transcription factor (MITF); RNA stability;
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