• 치위생과학회지
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• 제23권2호
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• pp.169-175
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• 2023

Background: Although microbial infection is direct cause of periodontal disease, various environmental factors influence the disease severity. Aging is considered a risk factor for oral diseases, with the prevalence of periodontal diseases increasing with age. Moreover, senescence-associated secretory phenotype (SASP) expressed in age-related diseases is a key marker of chronic inflammation and aging phenotypes. Therefore, this study aimed to understand the relevance of senescent cells to periodontal health and disease, investigate the possibility of regulating the expression of aging- and osteolysis-related factors in gingival fibroblasts, and investigate the effect of senescence induction in gingival fibroblasts on osteoclast differentiation in mouse bone marrow-derived macrophages (BMMs). Methods: After stimulation with 400 nM hydrogen peroxidase, human gingival fibroblasts (HGFs) were examined for senescence-associated β-galactosidase. Western blot and enzyme-linked immunosorbent assays were performed to assess the expression of SASP. Osteoclast formation was assessed in BMMs using a conditioned medium (CM) from hydrogen peroxide-stimulated HGFs. Osteoclastic differentiation was investigated using tartrate-resistant acid phosphatase (TRAP) staining and activity. Data analysis was performed using SPSS version 25.0. Results: The expression of senescence-related molecules, including p53, p16, and p21, and the expression of osteolytic factors, including IL-6, IL-8, and IL-17, were found to be significantly higher in the hydrogen peroxide-stimulated HGF than in the control group. Regarding the indirect effects of senescent gingival cells, the number of osteoclasts and TRAP activity increased according to the differentiation of BMM cultured in CM. Conclusion: Our results on the of between osteolytic factors and cellular senescence in gingival fibroblast cells helped to reveal evidence of pathological aging mechanisms. Furthermore, our results suggest that the development of novel therapies that target specific SASP factors could be an effective treatment strategy for periodontal disease.

• 치위생과학회지
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• 제23권4호
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• pp.264-270
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• 2023

Background: Resin-based dental materials release residual monomers or other substances from incomplete polymerization into the oral cavity, thereby causing adverse biological effects on oral tissue. 10-Methacryloyloxydecyl dihydrogen phosphate (10-MDP), an acidic monomer containing dihydrogen phosphate and methacrylate groups, is the most commonly used component of resin-based dental materials, such as restorative composite resins, dentin adhesives, and resin cements. Although previous studies have reported the cytotoxicity and biocompatibility in various cultured cells, the effects of resin monomers on cellular aging have not been reported to date. Therefore, this study aimed to investigate the effects of the resin monomer 10-MDP on cellular senescence and inflamm-aging in vitro. Methods: After stimulation with 10-MDP, MC3T3-E1 osteoblast-like cells were examined for cell viability by WST-8 assay and reactive oxygen species (ROS) production by flow cytometry. The protein and mRNA levels of molecular markers of aging were determined by western blotting and RT-PCR analysis, respectively. Results: Treatment with 0.05 to 1 mM 10-MDP for 24 hours reduced the survival of MC3T3-E1 cells in a concentration-dependent manner. The intracellular ROS levels in the 10-MDP-treated experimental group were significantly higher than those in the control group. 10-MDP at a concentration of 0.1 mM increased p53, p16, and p21 protein levels. Additionally, an aging pattern was observed with blue staining due to intracellular senescence-associated beta-galactosidase activity. Treatment with 10-MDP increased the levels of tumor necrosis factor-α, interleukin (IL)-1β, IL-6 and IL-8, however their expression was decreased by mitogen-activated-protein-kinase (MAPK) inhibitors. Conclusion: Taken together, these results suggest that the exposure of osteoblast-like cells to the dental resin monomer 10-MDP, increases the level of cellular senescence and the inflammatory response is mediated by the MAPK pathway.

• 복식
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• 제57권10호
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• pp.50-59
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• 2007

This study has been conducted to classify elderly men's feet with the three-dimensional body scanner data of Size Korea. It was intended to assist the manufacturing of the shoes that can appropriately perform the functions of feet, by providing the specific information about the shapes of elderly men's feet that are altered as a result of aging and shoe-wearing for a long time. The findings are as follows. 1. The investigations into the average and standard deviation of the measurements and index values for the elderly men's feet showed a large personal difference in the items of length and circumference. 2. The factors constituting the elderly men's feet were observed to be the size of foot width(Factor 1), the central angle of feet and the extension of toes(Factor 2), the size of ankle(Factor 3), the positions of lateral malleolus and pternion(Factor 4), and the position and size of medial malleolus(Factor 5). 3. The cluster analysis for the classification of elderly men's feet produced three types of them.

• 대한한의학방제학회지
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• 제25권1호
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• pp.1-10
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• 2017

Objectives : The fruit of Prunus mume Siebold & Zucc. has been used as an alternative medicine and functional food in Korea and Japan for preventive and therapeutic purposes. However, its molecular actions and mechanism on anti-inflammatory activity have not been clearly investigated. The aim of this study was to clarify the anti-inflammatory activity of the ethanol extract of P. mume fruit (EEPM) in lipopolysaccharide (LPS)-stimulated RAW 264.7 cells, and sought to understand the associated molecular mechanisms. Methods : Cytotoxicity was assessed by an MTT assay. The amount of nitric oxide (NO) production was determined by nitrite assay. The mRNA expression of inducible nitric oxide synthase (iNOS) was analyzed by RT-PCR. In addition, expression levels of iNOS, nuclear factor-erythroid 2-related factor 2 (Nrf2) and heme oxygenase-1 (HO-1) protein were detected by Western blotting. Results : Our data indicated that EEPM inhibited NO production in LPS-stimulated RAW264.7 cells in a concentration-dependent manner. At the mRNA and protein levels, EEPM suppressed LPS-induced iNOS expression. On the other hand, EEPM markedly enhanced HO-1 expression, which was associated with an induction and nuclear translocation of Nrf2. Moreover, the inhibitory effect of EEPM against LPS‑induced NO production was significantly enhanced by hemin, a HO-1 inducer; however, EEPM's effect on the production of NO was abolished by zinc protoporphyrin IX, a HO-1 inhibitor. Conclusion : The results suggest that EEPM can act as a suppressor agent on NO production through an activation of Nrf2/HO-1 signaling pathway, and may be a promising candidate for the treatment of inflammatory diseases.

• 한국융합학회논문지
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• 제11권8호
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• pp.323-330
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• 2020

본 연구는 12주간의 규칙적인 신체활동 참여가 여성노인의 노화관련 호르몬(성장호르몬, DHEA-S, 에스트로겐) 및 심혈관 위험요소(아디포넥틴, hs-CRP)에 어떠한 영향을 미치는지 알아보는데 그 목적이 있다. 연구대상자는 65세-75세 여성노인 20명(운동집단 10명, 비교집단 10명)을 대상으로 하였다. 주 3회(60분씩) 12주간 한국무용 프로그램을 실시하였고 집단내 차이검증은 paired t-test로, 집단별 항목들의 변화량 차이 검증은 independent t-test를 실시하였다(p<0.05). 본 연구의 결과 에스트로겐(p=0.025), 성장호르몬(p=0.009), DHEA-S(p=0.009), 아디포넥틴(p=0.014)은 운동집단에서 운동 12주 후 유의하게 증가하였고 hs-CRP(p=0.010)는 운동 12주 후 유의하게 감소하였다. 에스트로겐(p=0.006), DHEA-S(p=0.002), 아디포넥틴(p=0.008), hs-CRP(p=0.004)는 집단간 변화량의 차이도 나타났다.

• 전기학회논문지
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• 제65권5호
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• pp.819-824
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• 2016

The accelerated thermal aging of CSPE (chlorosulfonated polyethylene) was carried out for 33.64 and 67.27 days at 110[$^{\circ}C$], equivalent to 40 and 80 years of aging at 50[$^{\circ}C$], respectively. These samples were referred to as CSPE-0y, CSPE-40y and CSPE-80y, respectively. As the accelerated thermally aged years of the CSPE increase, the insulation resistance[$\Omega$] at 20[Hz], 500[Hz], and 2[KHz], and the percent elongation [%EL] of the CSPE decrease. However, the dissipation factor($tan{\delta}$) at 20[Hz], 500[Hz], and 2[KHz], the apparent density[$g/cm^3$], the glass transition temperature and the melting temperature of the CSPE were increased. The period of time that the voltage has to be applied until electric breakdown of the CSPE-0y is longer than that of the CSPE-40y, and the CSPE-80y, but the dielectric strength of the CSPE-80y is lower than that of the CSPE-0y and the CSPE-40y. The differential temperatures after the AC and DC voltages are applied to CSPE-0y, CSPE-40y and CSPE-80y are 0.026~0.028[$^{\circ}C$], 0.030~0.042[$^{\circ}C$], 0.018~0.045[$^{\circ}C$], respectively. The variations of temperature for the AC voltage are higher than those for the DC voltage when an AC voltage is applied to CSPE-0y, CSPE-40y and CSPE-80y. It is found that the dielectric loss owing to the dissipation factor[$tan{\delta}$] is related to the electric dipole conduction current. It is ascertained that the ionic (electron or hole) leakage current is increased by the separation of the branch chain of CSPE polymer from the main chain of the polyethylene as a result of thermal stress due to accelerated thermal aging as well as by conducting ions such as $Na^+$, $Cl^-$, $Mg^{2+}$, $SO_4^{2-}$, $Ca^{2+}$ and $K^+$ after seawater soaking.

• 한국노년학
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• 제29권3호
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• pp.1063-1085
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• 2009

본 연구는 시간이 경과함에 따른 노인들의 복지서비스 이용경험의 변화를 살펴보고, Andersen과 Newman(1973)의 행동주의 모델을 적용하여 서비스 이용경험에 영향을 미치는 요인들을 밝힘과 동시에 지속적으로 영향을 미치는 요인을 밝히고자 시도되었다. 이를 위해 2003년부터 2007년까지 세 차례에 걸쳐 수집된 한림고령자패널자료 중 60세 이상 노인을 분석대상으로 경로우대서비스, 여가서비스, 재가복지서비스 이용경험과 Andersen과 Newman(1973)의 모델에서 제시된 선행요인(성, 연령, 교육수준), 가능성요인(거주지역, 배우자유무, 월 용돈, 서비스 인지수준), 욕구요인(일상생활동작수행능력)에 해당하는 문항들을 분석하였다. 연구문제를 해결하기 위해 자귀회귀모형과 회귀분석을 결합한 모형을 만들고 이를 구조방정식 모형을 이용하여 분석하였다. 분석결과 첫째, 시간이 흐름에 따라 노인들은 경로우대서비스를 점차 보편적으로 이용하게 되었지만 여가서비스와 재가복지서비스를 이용함에 있어서는 아직까지 제한적이었다. 둘째, 경로우대서비스와 여가서비스에 대한 이용경험은 다음의 서비스 이용경험에 정적인 영향을 미치고 있었다. 셋째, 서비스 이용경험에 영향을 미친 요인들이 조사시점마다 달라졌지만, 경로우대서비스 이용에는 선행요인인 연령과 가능성요인인 거주지역, 여가서비스 이용에는 가능성요인인 거주지역과 배우자유무, 그리고 재가복지서비스 이용에는 선행요인인 교육수준과 가능성요인인 서비스 인지수준이 5년 동안 지속적으로 영향을 미치는 것으로 나타났다. 특히 선행연구들에서 서비스 이용에 욕구요인이 중요한 요인으로 지목되어온 것과는 달리 본 연구에서는 가능성요인이 중요하게 부각되었다. 이러한 결과를 통해 노인 개인이나 가족의 특성보다는 서비스를 공급하는 지역의 복지환경이 노인의 서비스 이용에 더 영향을 미침을 확인하였다. 따라서 노인의 노인복지서비스 이용을 확대하기 위해서는 경로우대서비스와 여가서비스에 대한 복지자원과 재정에 있어서의 지역 간 격차를 줄이고 재가복지서비스에 대한 홍보를 체계화하는 노력이 요구되었다.

• 대한가정학회지
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• 제41권6호
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• pp.1-12
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• 2003

The purpose of this study were to analyze the anthropometric data of feet of Korean women with aging, to categorize the women's foot shapes, and to compare the shoe size according to the foot shapes in order to provide the basic information for more comfortable shoes. Subjects of this study were 181 women over age 20. They were measured with the direct measurement method and the indirect measurement method. 26 items were measured from the right foot and 6 items were taken on foot outline. Factor analysis, cluster analysis, analysis of variance, post-hoc test, and cross tabs were peformed for statistical analysis of the data by SPSS program. There were significant differences in height items, breadth items, girth items, and angle items by subjects' age. The older subjects' feet were wide and thick with big deformity on toes. The arch height of the older ones was low. This implicates that the degree of deformity on toes, the foot ratio, the foot girth, the foot breath and the arch height as well as the foot length are needed to be considered in developing comfortable shoes. Nine foot construction factors were extracted by the factor analysis of anthropometric measurements; foot size factor, heel and instep factor, malleolus lateralis factor, malleolus medialis factor, foot shape factor, shape of toes factor, heel height factor, big toe height factor, and internal factor. On the basis of the cluster analysis, three different foot shapes were categorized. Type 1 was large and wide foot with little deformity on little toe. Type 2 was medium foot with deformation of big toe, and with the lowest arch height. Type 3 was small and narrow foot with the highest arch height. Distribution of shoe size according to the foot shape was analyzed. The ball of foot breath was of wide distribution than the ball of foot girth. This implicates that girth items and breath items of the foot should be enclosed for the same foot length in the shoe sizing system.

• Journal of Ginseng Research
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• 제37권1호
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• pp.54-63
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• 2013

Ginsenoside Rd is a primary constituent of the ginseng rhizome and has been shown to participate in the regulation of diabetes and in tumor formation. Reports also show that ginsenoside Rd exerts anti-oxidative effects by activating anti-oxidant enzymes. Treatment with ginsenoside Rd decreased nitric oxide and prostaglandin $E_2$ ($PGE_2$) in lipopolysaccharides (LPS)-challenged RAW264.7 cells and in ICR mouse livers (5 mg/kg LPS; LPS + ginsenoside Rd [2, 10, and 50 mg/kg]). Furthermore, these decreases were associated with the down-regulations of inducible nitric oxide synthase (iNOS) and cyclooxygenase (COX)-2 and of nuclear factor (NF)-${\kappa}B$ activity in vitro and in vivo. Our results indicate that ginsenoside Rd treatment decreases; 1) nitric oxide production (40% inhibition); 2) $PGE_2$ synthesis (69% to 93% inhibition); 3) NF-${\kappa}B$ activity; and 4) the NF-${\kappa}B$-regulated expressions of iNOS and COX-2. Taken together, our results suggest that the anti-inflammatory effects of ginsenoside Rd are due to the down-regulation of NF-${\kappa}B$ and the consequent expressional suppressions of iNOS and COX-2.

• 한국노년학
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• 제39권3호
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• pp.589-612
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• 2019

본 연구는 급속한 근대화와 세계화 과정을 거치면서 한국의 전통적인 가치관의 변화가 한국노인들의 노화경험에 대한 인식에 어떠한 영향을 미치는지에 대해 알아보고자 하였다. 사회적, 문화적인 변화가 한국노인의 노화 경험에 어떠한 영향을 미치는지 이해하기 위해 한국의 도시, 농촌, 그리고 미국이라는 세 가지 지리적 위치에 거주하는 한국노인들의 노화 경험의 유사점과 차이점을 다차원적으로 비교분석하였다. 본 연구의 수행을 위하여 한국노인 30명을 대상으로 생애인터뷰를 하였으며, 신체적 쇠퇴에도 불구하고 노년기에 의미 있고 탄력 있는 삶을 사는 심리적, 사회적, 정서적, 영적 경험을 분석하는데 중점을 두었다. 분석결과 첫째, 내러티브 분석을 토대로 노화 경험에 긍정적, 부정적 영향을 미치는 영역으로 신체적 건강, 정신적 건강, 가족관계, 일, 여가/사회적 활동, 영성 요인이 도출되었다. 둘째, 주제분석을 토대로 공통적으로 도출된 노화의 요인은 '자식들에게 빚지지 않는 삶', '신체적, 정신적 건강', '잘 죽는 것'으로 나타났다. 특히 신앙은 노년기 삶에 대한 관점을 이해하고 받아들이는 데 중요한 역할을 하였으며, 특정한 종교적 신념에 뿌리를 두고 있었다. 셋째, 한국노인과 재미 한인노인 간에 '일이 갖는 의미'와 '여가선호 방식'에 차이가 드러났다. 넷째, 농촌지역에 거주하는 한국노인들에게 부정적인 영향을 미치는 요인은 교육을 받지 못한 수치심과 관련이 있었다. 이와 같은 결과를 바탕으로 후속연구에 대한 함의점을 제시하였다.