• 한국컴퓨터정보학회:학술대회논문집
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• 한국컴퓨터정보학회 2011년도 제44차 하계학술발표논문집 19권2호
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• pp.363-366
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• 2011

3D 디스플레이 시장이 커지면서 TFT-LCD TV 자동화 라인 생산 공정을 3D LCD TV 생산 공정으로 변환하여 대량 생산할 경우, 기 설치된 자동화 생산라인의 H/W 부분에는 특별한 투자가 이루어질 거라 판단되지 않고 있다. 하지만 3D LCD Module 검사 등의 S/W 적인 부분은 현재의 기 설치된 TFT-LCD Module 검사장비로는 검사가 불가능하여, 추가적인 투자가 이루어질 것이라 판단되고 있다. 이와 관련하여 본 연구에서는 TFT-LCD 제조공정의 효율적인 기술을 제안하고자 한다. 첫째는 TFT-LCD를 Cell상태에서 직접 구동하는 구동회로 기술과 사용 장소가 Clean Room이므로 개별적 구동 Pallet에 비접촉식으로 전원을 공급할 수 있는 비접촉식 급전장치에 대한 기술이다.

• 대한의생명과학회지
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• 제27권4호
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• pp.231-238
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• 2021

Several structural proteins present in keratinocytes of the skin are known to play an important role in the formation of epidermal tissue and barrier function, and the absence of structural proteins in keratinocytes causes various skin diseases. In this study, 42 types of tetrapeptides derived from the sequence of Loricrin, a kind of terminally differentiating structural protein, were synthesized, and skin anti-aging properties were measured by measuring the elastase inhibition, proliferation of skin cells. The anti-aging efficacy was verified and, based on this, it succeeded in selecting one of the most excellent peptides. It is expected that the selected tetrapeptide can be used as a raw material for various cosmetics and quasi-drugs based on anti-aging and skin cell proliferation effects.

• 생명과학회지
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• 제30권9호
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• pp.819-825
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• 2020

모든 생명체는 연령이 증가함에 따라 생체 내에서 노화된 세포수가 축적되면서 피부주름 형성, 근육퇴화, 백내장 및 모발의 백발화 과정 같은 노화의 특성을 나타낸다. 이러한 노화의 핵심적인 원인으로 알려진 세포노화는 세포가 외부 및 내부요인에 의하여 늙어서 결국 세포의 증식이 정지됨으로 생물체의 노화와 직접적으로 밀접하게 연관되어 있다. 이러한 현상에 대한 보다 심층적인 연구로부터 세포노화의 원인이 텔로미어가 세포 분열에 따라 점차적으로 짧아짐으로, 텔로미어의 길이에 의해서 결정된다는 것이다. 최근에는 유전자 발현에 영향을 줄 수 있는 히스톤 디아세틸레이즈 유전자가 효모에서 뿐만 아니라 예쁜 꼬마 선충 및 사람의 항노와 기전에 깊숙히 관여하고 있다는 것이 밝혀졌다. 한편, 최근에는 늙은 세포가 노화 현상에 결정적인 역할을 한다는 것이 발견하여 이러한 노화세포를 채내로부터 제거함으로써 젊은 세포의 증식을 촉진하여 노화를 지연 할 수 있다는 것이 보고되었다. 그러므로 향후 잠재적인 항노화제를 개발하기 위해서는 텔로머레이즈 활성화제, 서르튜언 활성화제, 세노릭틱스에 대한 심층연구로부터 시작되어야 한다고 판단되어, 최근에 각광 받고 있는 위와 관련된 항노화 후보물질에 대한 최근 연구에 대하여 기술한다.

• Applied Microscopy
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• 제25권1호
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• pp.1-14
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• 1995

The purpose of this study was to investigate the main sensory trigeminal nucleus in the aging rat brain by means of electron microscope. Male Sprague-Dawley rats, two (control group) and thirty six (aging group) months of age, were used. These animals were sacrificed by perfusion fixation with 2.5% glutaraldehyde-2.0% paraformaldehyde (0.1M phosphate buffer, pH 7.4) under sodium pentobarbital. The objective area was punched out with a sharp-edged metal cylinder of 0.8 mm in diameter. These blocks of tissue were then washed in 0.1M phosphate buffer, postfixed in 2% osmium tetroxide, dehydrated in a graded series of ethyl alcohol, and embedded in Epon 812. Thin sections were cut with Super Nova ultramicrotome, pick up on grids and double stained with lead citrate and uranyl acetate, and observed in JEOL 100B electron microscope. The results were as follows: 1. In the control group, the neuronal cell body of the main sensory trigeminal nucleus was filled with nucleus, Golgi complex, Nissl substance, mitochondria, microfilaments and microtubules. However, few Nissl substances are seen in neuronal cell body. Axoaxonic synapse, axodendritic synapse, axosomatic synapse, axospinous synapse, myelinated and unmyelinated nerve fibers were well organized around cell bodies. Neurons with abnormal changes were not seen. 2. In the aging group, the neuronal cell body of the main sensory trigeminal nucleus contained large number of lipofuscin granules, dense body and swollen mitochondria. Terminal boutons contained glycogen, crystal-like vesicle and membranous indicating first signs of degeneration. The dendrites were found to be in synaptic contact with altered axon terminals. Frequently axons filled with dark axoplasn and splitted myelin sheath were noticed.

• Macromolecular Research
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• 제15권4호
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• pp.315-323
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• 2007

The present manuscript deals with the prediction of the lifetime of NBR compound based rubber gaskets for use as fuel cells. The material was investigated at 120, 140 and $160^{\circ}C$, with aging times from 3 to 600 h and increasing $H_2SO_4$ concentrations of 5, 6, 7 and 10 vol%. Both material and accelerated acid-heat aging tests were carried out to predict the useful life of the NBR rubber gasket for use as a fuel cell stack. To investigate the effects of acid-heat aging on the performance characteristics of the gaskets, the properties of the NBR rubber, such as crosslink density and elongation at break, were studied. The hardness of the NBR rubber was found to decrease with decreasing acid concentration at both $120\;and\;140^{\circ}C$, but at $160^{\circ}C$, the hardness of the NBR rubber increased abruptly in a very short time at different acid concentrations. The tensile strength and elongation at break were found to decrease with increases in both the $H_2SO_4$ concentration & temperature. The observed experimental results were evaluated using the Arrhenius equation.

• 생명과학회지
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• 제29권10호
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• pp.1071-1079
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• 2019

본 연구에서 우리는 오미자 종자 분획물의 항산화 활성과 자외선에 유도된 human HaCaT cell에서의 MMPs의 발현억제에 의한 항노화 효과를 조사하고자 하였다. 전자공여능, ABTS 라디칼 소거분석, 그리고 hydrogen peroxide 소거분석 실험을 통해, 오미자 종자의 분획물 중 에틸아세테이트 분획물(SCEAf)이 가장 우수한 라디칼 소거활성을 가졌고, collagenase 저해 활성 실험에서 SCEAf는 $500{\mu}g/ml$의 농도에서 92.3% 이상의 저해효과를 보였음을 확인하였다. SCEAf의 HaCaT cell에 대한 세포 독성을 확인하기 위해 MTT assay를 실시한 결과, SCEAf는 세포에 독성이 없음을 나타낼 뿐 아니라 UVB에 손상된 세포의 생존율을 증가시킴으로써 세포 활성에 도움을 주는 것을 확인할 수 있었다. SCEAf의 HaCaT cell에서의 항노화 효과를 조사하기 위해 UVB $50mJ/cm^2$에 유도된 HaCaT cell에 SCEAf를 처리한 후 MMP-1과 -3의 발현을 Western blotting과 reverse-transcription polymerase chain reaction으로 분석하였다. 그 결과 SCEAf가 MMP-1과 -3의 단백질과 mRNAs의 발현을 농도의존적으로 억제시켰다. 이러한 결과는 오미자 종자 에틸아세테이트 분획물이 자외선의 손상을 받은 피부 각질형성세포에서의 collageanse 활성을 억제하여 노화를 예방하고 증상을 완화시킬 것으로 기대할 수 있다. 이러한 결과를 바탕으로 우리는 오미자 종자가 화장품과 식품 산업에서 항노화 효과가 있는 기능성 원료로서 사용하기 위한 잠재성을 가질 것으로 기대한다.

• Molecules and Cells
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• 제20권3호
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• pp.429-434
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• 2005

Lysophosphatidylcholine (lysoPC) induces vascular smooth muscle cell (VSMC) proliferation and migration, which has been proposed to initiate the intimal thickening in coronary atherosclerotic lesions. Berberine is an alkaloid in Berberis aquifolium and many other plants. Recently, it has been shown to have beneficial effects on the cardiovascular system, such as anti-hyperglycemic and cholesterol-lowering activity. In this study, we investigated its effects on lysoPC-induced VSMC proliferation and migration. Berberine inhibited lysoPC-induced DNA synthesis and cell proliferation in VSMCs, as well as migration of the lysoPC-stimulated VSMCs. It also inhibited the activation of extracellular signal-regulated kinases (ERKs) and reduced transcription factor AP-1 activity and the lysoPC-induced increases in intracellular reactive oxygen species (ROS). These results indicate that the inhibitory effects of berberine on lysoPC-stimulated VSMC proliferation and migration are attributable to inhibition of ROS generation and hence of activation of the ERK1/2 pathway. This suggests that berberine has potential in the prevention of atherosclerosis and restenosis.

• 대한약침학회지
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• 제18권2호
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• pp.26-32
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• 2015

Objectives: Rheum undulatum L. has traditionally been used for the treatment of many diseases in Asia. However, its anti-proliferative activity in cancer has still not been studied. In the present study, we investigated the anti-cancer effects of methanol extract of Rheum undulatum L. (MERL) on human adenocarcinoma gastric cell lines (AGS). Methods: To investigate the anti-cancer effect of MERL on AGS cells, we treated the AGS cells with varying concentrations of MERL and performed 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide (MTT) assays. Cell cycle analyses, measurements of the mitochondrial membrane potential (MMP), caspase activity assays and Western blots were conducted to determine whether AGS cell death occurred by apoptosis. Results: Treatment with MERL significantly inhibited growth of AGS cells in a concentration dependent manner. MERL treatment in AGS cells leaded to increased accumulation of apoptotic sub G1 phase cells in a concentration dependent manner. In control cultures, 5.38% of the cells were in the sub G1 phase. In MERL treated cells, however, this percentage was significantly increased (9.95% at $70{\mu}g/mL$, 15.94% at $140{\mu}g/mL$, 26.56% at $210{\mu}g/mL$ and 38.08% at $280{\mu}g/mL$). MERL treatment induced the decreased expression of pro-caspase-8 and -9 in a concentration dependent manner, whereas the expression of the active form of caspase-3 was increased. A subsequent Western blot analysis revealed increased cleaved levels of poly (ADP-ribose) polymerase (PARP) protein. Also, treatment with MERL increased the activities of caspase-3 and -9 compared with the control. MERL treatment increased the levels of the pro-apoptotic truncated Bid (tBid) and Bcl2 Antagonist X (Bax) proteins and decreased the levels of the anti-apoptotic B-cell lymphoma 2 (Bcl-2) protein, whose is the stabilization of mitochondria. However, inhibitions of p38, extracellular signal regulated kinases (ERKs) and C-Jun N-terminal kinases (JNK) by MERL treatment did not affect cell death. Conclusion: These results suggest that MERL mediated cell death is associated with an intrinsic apoptotic pathway in AGS cells.

• BMB Reports
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• 제35권1호
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• pp.87-93
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• 2002

Neural cell survival is an essential concern in the aging brain and many diseases of the central nervous system. Neural transplantation of the stem cells are already applied to clinical trials for many degenerative neurological diseases, including Huntington's disease, Parkinson's disease, and strokes. A critical problem of the neural transplantation is how to reduce their apoptosis and improve cell survival. Neurotrophic factors generally contribute as extrinsic cues to promote cell survival of specific neurons in the developing mammalian brains, but the survival factor for neural stem cell is poorly defined. To understand the mechanism controlling stem cell death and improve cell survival of the transplanted stem cells, we investigated the effect of plausible neurotrophic factors on stem cell survival. The neural stem cell, HiB5, when treated with PDGF prior to transplantation, survived better than cells without PDGF. The resulting survival rate was two fold for four weeks and up to three fold for twelve weeks. When transplanted into dorsal hippocampus, they migrated along hippocampal alveus and integrated into pyramidal cell layers and dentate granule cell layers in an inside out sequence, which is perhaps the endogenous pathway that is similar to that in embryonic neurogenesis. Promotion of the long term-survival and differentiation of the transplanted neural precursors by PDGF may facilitate regeneration in the aging adult brain and probably in the injury sites of the brain.

• Biotechnology and Bioprocess Engineering:BBE
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• 제5권1호
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• pp.44-47
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• 2000

Cell growth and DNA synthesis were studied from a cultured early- and late- pas- sage mouse aorta smooth muscle cell (MASMC) because the proliferation of vascular smooth muscle cell (VSMC) is a key factor in development of atherosclerosis. In this study, the cells were cultured in fetal bovine serum (FBS) and stimulated by growth factors such as thrombin and platelet-derived growth factor-BB (PDGF-BB). Compared to the number of early-passage MASMC (passage 3 to 9) the number of late-passage MASMC (passage 30 to 40) in a normal serum state was increased 2 fold at Day 1, 3 and 6 in culture, respectively. Incorporation of $[^3H]$ thymidine into DNA induced by serum, PDGF and thrombin in late-passage MASMC was greater than those in early-passage MASMC. We also examined whether intracellular zinc levels would be an aging factor or not. The intracellular zinc level in early- and late-passage MASMC was monitored by using the zinc probe dye N-(6-methoxy-8-quinolyl)-p-toluenesulfonamide. It is interested that late-passage MASMC increased the intracellular fluorescence level of zinc, more than the early passage MASMC did. The alterations of intracellular zinc level occur concurrently with changes in MASMC proliferation rate during aging. This data suggest that the age-associated changes in zinc concentrations may provide a new in vitro model for the study of smooth muscle cell differentiation.