• Journal of Microbiology and Biotechnology
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• v.30 no.2
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• pp.226-236
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• 2020

Antibiotic resistance by pathogenic bacteria and fungi is one of the most serious global public health problems in the 21^st century, directly affecting human health and lifestyle. Pseudomonas aeruginosa and Staphylococcus aureus with strong resistance to the common antibiotics have been isolated from Intensive Care Unit patients at Zagazig Hospital. Thus, in this study we assessed the biocidal activity of nanoparticles of silver, copper and zinc synthesized by Fusarium solani KJ 623702 against these multidrug resistant-bacteria. The synthesized Metal Nano-particles (MNPs) were characterized by UV-Vis spectroscopy, transmission electron microscopy, Fourier transform infrared spectroscopy, X-ray diffraction, and Zeta potential. The Fourier transform infrared spectroscopy (FTIR) result showed the presence of different functional groups such as carboxyl, amino and thiol, ester and peptide bonds in addition to glycosidic bonds that might stabilize the dispersity of MNPs from aggregation. The antimicrobial potential of MNPs by F. solani against the multidrug-resistant (MDR) P. aeruginosa and S. aureus in addition to the mycotoxigenic Aspergillus awamori, A. fumigatus and F. oxysporum was investigated, based on the visual growth by diameter of inhibition zone. Among the synthesized MNPs, the spherical AgNPs (13.70 nm) displayed significant effect against P. aeruginosa (Zone of Inhibition 22.4 mm and Minimum Inhibitory Concentration 21.33 ㎍/ml), while ZINC oxide Nano-Particles were the most effective against F. oxysporum (ZOI, 18.5 mm and MIC 24.7 ㎍/ml). Transmission Electron Microscope micrographs of AgNP-treated P. aeruginosa showed cracks and pits in the cell wall, with internalization of NPs. Production of pyocyanin pigment was significantly inhibited by AgNPs in a concentration-dependent manner, and at 5-20 ㎍ of AgNPs/ml, the pigment production was reduced by about 15-100%, respectively.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.48 no.6
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• pp.960-968
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• 2015

This study examined the spatiotemporal distribution of demersal fish aggregations in the Northern East China Sea by conducting a trawl survey with hydroacoustic devices. A bottom trawl was used for this survey and fish density was determined from the catch data. Acoustic data were collected at frequencies of 38 and 200 kHz from November to December 2014 and converted into the nautical area scattering coefficient (NASC, $m^2/n{\cdot}mile^2$). In the catch data analysis, the range of catch per unit area by station was $26-8,055kg/km^2$ and for the acoustic data, that of the NASC was $0.45-34.80m^2/n{\cdot}mile^2$. The values were significantly correlated. The combined results of both surveys found that the density was highest at St. 5 ($33^{\circ}$ 10.3', $126^{\circ}$ 23.3') and lowest at St. 8 ($33^{\circ}$ 20.7', $127^{\circ}$ 36.3'). The application of hydroacoustic methods offers a new approach for estimating the biomass of demersal fish aggregations.

• The Journal of Korean Institute of Communications and Information Sciences
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• v.40 no.8
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• pp.1613-1621
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• 2015

This paper analyzes the rate adaptation scheme and suggests applicable strategy of the MCS(Modulation and Coding Scheme) for improving DCF throughput in the IEEE 802.11ad and 802.11ad wireless LAN. IEEE 802.11ac and 802.11ad wireless LAN provide MCS technique that dynamically adjusts modulation level and code rate to the time-varying channel conditions in order to obtain considerably high data rates. But these standards did not provide rate adaptation algorithm, so this paper surveyes rate adaptation algorithm and suggests MCS scheme applied to IEEE 802.11ac and 802.11ad wireless LAN. Specially A MAC(Medium Access Control) layer throughput is evaluated over error-prone channel in the IEEE 802.11ac-based wireless LAN. In this evaluation, DCF (Distributed Coordination Function) protocol and A-MPDU (MAC Protocol Data Unit Aggregation) scheme are used. Using theoretical analysis method, the MAC saturation throughput is evaluated with the PER (Packet Error Rate) on the condition that the number of station, transmission probability, the number of parallel beams and the number of frames in each A-MPDU are variables.

• Proceedings of the Korean Society of Applied Pharmacology
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• 1996.11a
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• pp.75-84
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• 1996

Platelets from a patient with a mild inherited bleeding disorder and abnormal platelet aggregation and secretion show reduced generation of inositol 1,4,5-trisphosphate (IP$_3$), mobilization of intracellular Ca$\^$2+/, and phosphorylation of pleckstrin in response to several G protein mediated agonists, suggesting a possible defect at the level of phospholipase C (PLC) activation. A procedure was developed that allows quantitation of platelet PLC isozymes. After fractionation of platelet extracts by high-performance liquid chromatography, seven, out often known PLC isoforms were detected by immunoblot analysis. The amount of these isoforms in normal platelets decreased in the order PLC-${\gamma}$2 > PLC-${\beta}$2 > PLC-${\beta}$3 > PLC-${\beta}$l > PLC-${\gamma}$ > PLC-$\delta$1 > PLC-${\beta}$4. Compared with normal platelets, platelets from the patient contained approximately one-third the amount of PLC-${\beta}$2, whereas PLC-${\beta}$4 was increased threefold. These results suggest that the impaired platelet function in the patient in response to multiple G protein mediated agonists is attributable to a deficiency of PLC-${\beta}$2. They document for the first time a specific PLC isozyme deficiency in human platelets and provide an unique opportunity to understand the role of different PLC isozymes in normal platelet function.

• The Sea:JOURNAL OF THE KOREAN SOCIETY OF OCEANOGRAPHY
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• v.6 no.3
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• pp.164-179
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• 2001

Spatio-temporal variation in species composition, abundance and distribution of fisheries resources caught by large purse seine off Korea from 1991 to 1994 was analysed. Out of 19 species, Scomber japonicus (54.7%), Sardinops melanostictus (20.4%), Trachurus japonicus (9.1%), and Scomberomorus niphonius (6.2%) were predominated in catch. These 4 pelagic fishes occupied 90% of total catch. Major pelagic fishes caught by large purse seine were dispersed to northward from spring to summer, and migrated to the southern wintering ground during winter. Fishing effort (number of haul) was high in summer, but catch per unit effort showed a peak in winter. The high CPUE in winter seemed to be related to aggregation of the pelagic fish to the wintering ground in the southern sea. After spawning, they were widely dispersed for feeding in warm months.

• Journal of the Korea Institute of Information and Communication Engineering
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• v.16 no.11
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• pp.2437-2445
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• 2012

This paper presents the efficient measurement method of radio resource by analyzing various medium occupied elements. The medium occupied time consists of 802.11 frames, wireless interference, and protocol waiting time from a wireless node on a current channel. And it is used to performance metric. Existing research is only measured partial occupied elements, and is lack of validation of measurement unit and scalability on various IEEE 802.11 radio. This paper presents the measurement method of classified occupied elements. To achieve this, we modified 802.11n based OpenHAL device driver to collect the register information of wireless chipset, and to analyze receiving frames in an virtual monitor mode. We conclude accurate medium occupied time measurement system from various validation methods.

• Journal of the Korea institute for structural maintenance and inspection
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• v.22 no.4
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• pp.60-67
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• 2018

As the lack of specific aggregation intensifies, the development of alternative resources is urgent. Construction waste is increasing every year, but recycled aggregate is used as a low value added material. Various studies are currently underway at the national level. In this paper, the mechanical performance of the concrete according to the concrete mixing method and the replacement amount of the circulating coarse aggregate was compared and evaluated. Concrete mixing method was normal mixing approach(NMA) method, two-stage mixing approach1 (TSMA1) method, two-stage mixing approach2 (TSMA2) method. Fresh concrete was tested for air content, slump test, and unit volume weight. Compressive strength and flexural strength were tested in hardened concrete. According to the TSMA method, the mechanical performance difference of concrete is shown, and the strength is decreased according to the circulating coarse aggregate replacement amount.

• Journal of Life Science
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• v.13 no.6
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• pp.814-821
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• 2003

Protoporphyrin IX is an intermediate molecule in the heme biosynthetic pathway. Intra- and extracellular concentrations of protoporphyrin IX in the wild type strain, Myxococcus xanthus DK1622 were measured by reverse phase HPLC. The amount of intracellular protoporphyrin IX continuously increased and reached 6.4 picomoles/mg of protein at the stationary phase. Extracellular protoporphyrin IX began to be detected from the mid-exponential phase. The culture supernatant that was collected in the stationary phase contained approximately 3.0 picomoles of proto-porphyrin IX per mg of protein. Spores formed by nutrient depletion contained about 6.5 picomole protoporphyrin IX/mg of protein. The synthesis of protoporphyrin IX and cell growth were strongly inhibited by addition of succinylacetone to a final concentration of $500\muM$. Succinylacetone, however did not appear to interfere developmental processes. Normal developmental behaviors including aggregation and spore formation was achieved even if succinylacetone was added in a medium. Photolysis among cells grown on a starvation medium supplemented with succinylacetone was also observed. These results indicate that protoporphyrin IX may be important to M. ,xanthus vegetative growth, but not critical to development processes.

• The Journal of the Korea Contents Association
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• v.19 no.6
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• pp.236-248
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• 2019

Green belts can help to set boundary for city growth, provide ecological protection system and enhance the resilience of Beijing. During the implementation period of the current overall city planning of Beijing, the proportion of green space varies much among the sub-districts, villages and towns involved in the two green belts in the research. With this as starting point, by researching the correlations of 'city system factors' and 'planning policy factors' with the change in the scale of green space, the paper discussed the influential factors of implementing the planning of green space of Beijing, and conducted quantitative research, with such possible influential factors classified into 'city system factors' and 'planning policy factors'. Through multiple linear regression model, the paper tested the correlations of city system factors and planning policy factors (independent variable) with the increment in the construction land in green belts (dependent variable). Through influence to population aggregation and the expansion force of construction land, city system factors such as mountain land and water, house rent of unit area, accessibility of public transport and the newly-defined state-owned construction land generate correlation with the change in the scale of construction land in green belts.

• Korean Journal of Microbiology
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• v.43 no.4
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• pp.298-303
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• 2007

Antithrombotic activities of water extract of cheongkookjang and cheongkookjang fermented with green tea or mugwort were evaluated on some antithrombosis related activities in vitro and thrombotic death inhibition in vivo. Cheongkookjang made of white soybean (Glycine max) or black small soybean (Rhynchosia nulubilis) showed potent antioxidative activities. Addition of green tea or mugwort during cheongkookjang fermentation increased the antioxidative activity, cheongkookjang with green tea showed more drastic increase compared with cheongkookjang with mugwort. Nitrite scavenging effects of the cheongkookjang extracts were prominent but the addition of green tea or mugwort seldom increased the scavenging effects. All the cheongkookjang extracts showed strong inhibitory activities on platelet aggregation. The inhibitory activities of cheongkookjang were increased considerably by addition of green tea or mugwort even with low concentration. Plasmin unit as fibrinolytic activity was not affected considerably by addition of green tea. Addition of mugwort decreased the activity transiently at low concentration ($0.3{\sim}1.0%$) but increased again slowly at higher concentration ($1{\sim}3%$). In vitro thrombotic death inhibition test, the antithrombotic activity of cheongkookjang made of black small bean with green tea was higher by about 1.5 times compared to that without green tea. As results, cheongkookjang might inhibit antithrombosis not only by fibrinolytic action but also by inhibition of platelet aggregation and antioxidative action. The addition of functional materials such as green tea or mugwort could increase the antithrombotic function, even at low concentration.