• The Korea Journal of Herbology
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• v.34 no.2
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• pp.67-74
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• 2019

Objectives : Agastachis herba is a aerial part of Agastache rugosa (Fischer et Meyer) O. Kuntze which has been used as traditional medicines. Methods : The samples were collected using a domestic cultivation of Agastachis Herba. We experiment on essential oil content, the content of ethanol soluble extracts and HPLC profiling. Results : Loss on drying of Agastachis Herba contents of harvest in June ~ July was 4.93~8.26% and contents of harvest in August ~ September was 3.88~5.38%. Ash of Agastachis Herba contents of harvest in June ~ July was 5.07~7.97% and contents of harvest in August ~ September was 4.55~7.61%. Acid-insoluble ash of Agastachis Herba contents of harvest in June ~ July was 0.43~1.62% and contents of harvest in August ~ September was 0.46~1.44%. Essential oil contents of Agastachis Herba contents of harvest in June ~ July was $0.2{\sim}0.4m{\ell}$ and contents of harvest in August ~ September was $0.2{\sim}0.6m{\ell}$. Ethanol soluble extracts of Agastachis Herba contents of harvest in June ~ July was 9.72~20.44% and contents of harvest in August ~ September was 11.00~18.70%. HPLC profiling was detected of tilianin(12.93 min), acacetin(15.84 min) and agastachoside(17.95 min). Conclusion : Agastachis Herba should be harvested from August ~ September, the time of flowering. This is because of the high content of active compounds.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.22 no.4
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• pp.483-492
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• 2021

Agastachis Herba (AH) to treat anorexia and nausea and its antidiabetic efficacy was recently reported. This study examined the antioxidant activities and chemical constituents of AH and predicted the target proteins of each compound using in silico approaches. The results showed that EC50 values of AH methanol extract for DPPH and ABTS radical scavenging were 78.6 ㎍/mL and 31.0 ㎍/mL, respectively. Compared to the EC50 values of ascorbic acid (9.9 ㎍/mL, 5.2 ㎍/mL), the AH methanol extract possessed excellent antioxidant activities. Rosmarinic acid, tilianin, agastachoside, and acetin were confirmed as the major compounds of extracts by qualitative analysis performed with HPLC-PDA-MS/MS. The antidiabetic target proteins of these compounds were predicted by applying a structural similarity and inverse docking methodology using a DIA-DB server. The resulting target proteins were PPAR-γ, DPP IV, glucokinase, α-glucosidase, SGLT2, aldose reductase, and corticosteroid 11-beta-dehydrogenase, some of which have already been proven experimentally as target proteins. Therefore, the in silico methods can be considered valid. Finally, AH were extracted with various solvents to determine the optimal conditions for the extraction of active components. Methanol among organic solvents and 80% ethanol in ethanol-water mixtures were identified as the most effective solvent for the extraction.