• Journal of Pharmaceutical Investigation
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• 제19권1호
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• pp.9-14
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• 1989

In order to study the protease from Agaricus bisporus (Lange), the crude protease preparation was separated by fractionation of mushroom extracts with ammonium sulfate. It was found that extracts from Agaricus bisporus (Lange) Sing. contained protease. The optimum pH of the enzyme was 6.0, and the pH range at which the enzyme was stable was 4.0 to 7.0. The optimum temperature at which the enzyme showed the highest proteolytic activity was $50^{\circ}C$, while the enzyme was instantly inactivated at about $60^{\circ}C$. The enzyme activity was inhibited by $Ag^+$, $Hg^{2+}$, $Cu^{2+}$, $Ba^{2+}$, $Fe^{3+}$, $Co^{2+}$, $Ca^{2+}$, $Pb^{2+}$, $Mg^{2+}$ and $Mn^{2+}$. The $K_m$ value was 0.32 mM with Hammarsten casein.

• 혜화의학회지
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• 제9권1호
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• pp.193-200
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• 2000

Basidiomycete, Agaricus blazei murill has grown Brazil naturally. It was first cultivated 1997 in Korea. Proteoglycan or polysaccharide which have the effect of immunopotency and anticancer were extracted from it. From the mycelium of cultivated mushroom, were extracted lectin, linoleic acid, FIII-2-b, and simmilar to glucomannan from fruit body of Basidiomycete Agaricus blazei. Fruit body was more studied than mycelium. The experiments were most in vivo study. The effect were shown on S-180, MethA tumor, Ehrlich ascites carcinoma, Shionogi carcinoma 42, Meth A fibrosarcoma by transferation of tumor cell line, specially effectiveness on the S-180. About immunopotenciation, were shown as activation of NK cell, pancreatic T-cell, helper T cell, enhancement of population of cytotoxity T cell. It was effect on the MethA tumor cells in vitro cell cytotoxity and has induction of apoptosis. Forthermore cytotoxity of many other tumor cell line, aneiognensis, cell cyle studies will be needed.

• The Plant Pathology Journal
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• 제21권4호
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• pp.343-348
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• 2005

The partial nucleotide sequences of the genomic dsRNA mycoviruses infecting Pleurotus ostreatus (isolates ASI2596, ASI2597, and Bupyungbokhoe) and Agaricus blazei Murrill were determined and compared with those of the other dsRNA mycoviruses. Partial nucleotide sequences of the purified dsRNA from ASI2596 and ASI2597 revealed RNA-dependent RNA polymerase sequences that are closely related to Oyster mushroom isometric virus 2, while nucleotide sequences and the deduced amino acid sequence from dsRNA mycovirus infecting Agaricus blazei did not show any significant homology to the other dsRNA mycoviruses. Specific primers were designed for RT-PCR detection of these dsRNA viruses and were found to specifically detect each dsRNA virus. Northern blot analysis confirmed the homogeneity of RT-PCR products to each purified dsRNA. Altogether, our results suggest that these virus-specific primer sets can be employed for the specific detection of each dsRNA mycovirus in infected mushrooms.

• KSBB Journal
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• 제15권3호
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• pp.307-312
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• 2000

버섯에서 추출되는 생리활성물질얀 ${\beta}$-glucan은 탁월한 항암 효과를 갖는 물질이다. 특히 Agaricus blazei에 $\beta$-glucan이 많이 포함되어 있는 것으로 보고되어져 있다. 본 연구의 목적은 마이 크로웨이브를 사용하여 아가리쿠스버섯으로부터 ${\beta}$-glucan을 추 출하는 공정의 추출효율을 극대화하는 것으로, 다양한 추출시간 과 마이크로웨이브 출력을 이용하여 최적 운전조건을 결정하였 다. 최적 마이크로웨이브의 최적 출력은 200, 500, 650 watt의 경우에서 실험하여 결정하였다. 추출물질의 구조와 농도 분석은 nuclear magnetic resonance spectroscopy와 infrared spectroscopy 를 이용하여 수행하였다 본 실험 결과로 마이크로웨이브를 이 용한 추출의 다당류 수율이 전통적 인 열수추출방법보다 향상됨을 확인하였고, 소량의 용매와 짧은 추출 시간으로 향상된 수율을 얻을 수 있음을 알 수 있었다.

• 한국식품과학회지
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• 제33권4호
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• pp.451-454
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• 2001

맛과 향기가 뛰어나고 고혈압 예방의 효과가 있어 식용으로 널리 이용되는 양송이(Agaricus bisporus)를 첨가한 고품질 어묵을 제조하여 색조 변화, 물성 특성 및 관능적 특성을 조사하였다. 양송이의 함유량이 증가할수록 어묵의 색도변화는 L값은 감소하는 경향, a값은 증가하는 경향, b값은 유의차가 없는 경향을 나타내었다. 또한, 어묵의 유연성을 나타내는 절곡검사의 결과로는 모든 시료에서 AA로 측정되어 양송이의 첨가에 관계없이 우수한 것으로 나타났다. 물성검사 결과에서, 양송이가 첨가될수록 어묵의 경도, 탄성, 껌성 및 파쇄성이 증가되었으며, 강도는 유의차가 보이지 않았다. 관능검사의 결과로 전체적인 선호도는 10%의 양송이를 함유한 어묵이 가장 높았다. 이상의 결과로 양송이를 함유한 고품질 어묵의 제조 가능성을 확인하였다.

• 한국버섯학회지
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• 제2권1호
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• pp.1-3
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• 2004

큰양송이(포토벨라)의 발이 및 생육에 적정한 온도를 구명코자 수행한 결과는 다음과 같다. 1. 초발이 소요일수는 $15^{\circ}C$에서 23일로 가장 짧았으며 그 다음은 $18^{\circ}C$, $21^{\circ}C$ 순이었다. 2. 자실체 발생 개체수는 $15^{\circ}C$에서 2,364개/$3.3m^2$로 가장 많았으나, 수량은 $18^{\circ}C$처리구에서 $39.2kg/3.3m^2$로 가장 높았다. 3. 온도별 자실체특성은 $21^{\circ}C$에서 상품(上品)비율이 75.1%로 $15^{\circ}C$, 24.5%와 $18^{\circ}C$, 48.9%보다 양호하였다. 4. 수량은 $15^{\circ}C$와 $18^{\circ}C$에 있어서 평당 36.5~39.2kg으로 비슷하였으나 $18^{\circ}C$에서 상품비율이 48.9%로 높아 100평당 소득은 16,013천원으로 높았다.

• 한국식품영양학회지
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• 제23권3호
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• pp.376-380
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• 2010

본 연구는 버섯에 정미 성분 및 기능성 성분으로 작용하는 핵산 관련 성분의 분석을 위하여 느타리, 양송이, 팽이버섯을 동결 건조한 뒤 열수 추출 및 효소적 가수분해과정을 거친뒤 HPLC로 정성 및 정량하였다. 연구 결과, 8종류의 핵산 관련 성분의 정성 및 정량 분석을 위한 효과적인 분석 조건을 확립하였으며, 버섯에 함유되어 있는 핵산 관련 성분은 cytidine, uridine, inosine, guanosine, 5'-UMP, 5'-GMP 및 5'-IMP 7종으로 분석되었다. 각각의 핵산 관련 성분 함량 분석 결과는 양송이 ($26.06{\pm}0.01\;mg/g$)>느타리($24.13{\pm}1.01\;mg/g$)>팽이버섯($18.84{\pm}0.03\;mg/g$)의 순으로 나타났으며, 그 중 cytidine 및 inosine 성분이 가장 많이 함유되어 있는 것으로 나타났다.

• Mycobiology
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• 제39권2호
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• pp.103-108
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• 2011

Amylases and cellulases are important enzymes that can be utilized for various biological activities. Ten different wild Nigerian mushrooms (Agaricus blazei, Agaricus sp., Corilopsis occidentalis, Coriolus versicolor, Termitomyces clypeatus, Termitomyces globulus, Pleurotus tuber-regium, Podoscypha bolleana, Pogonomyces hydnoides, and Nothopanus hygrophanus) were assayed for production of these secondary metabolites. The results revealed that most of the tested wild fungi demonstrated very good amylase and cellulase activities. With the incorporation of carboxymethyl-cellulose (a carbon source) into the culture medium, Agaricus blazei had the highest amylolytic activity of 0.60 unit/mL (at $25^{\circ}C$, pH 6.8). This was followed in order by P. tuber-regium and Agaricus sp. with 0.42 and 0.39 unit/mL, respectively ($p {\leq} 0.05$). Maltose and sucrose supplementation into the submerged liquid medium made N. hygrophanus and P. hydnoides to exhibit very low amylase activities of 0.09 and 0.11 unit/mL, respectively. Introducing peptone (an organic nitrogen source) into the basal medium enhanced the ability of C. versicolor to produce a cellulase value of 0.74 unit/mL. Other organic nitrogen sources that supported good cellulase activities were yeast extract and urea. Sodium nitrate (inorganic nitrogen source) generally inhibited cellulase production in all mushrooms. The best carbon source was carboxymethyl-cellulose, which promoted very high cellulase activity of 0.67 unit/mL in C. versicolor, which was followed in order by P. tuber-regium, T. chypeatus, and C. occidentalis ($p {\leq} 0.05$). Sucrose was the poorest carbon compound, supporting the lowest values of 0.01, 0.01, and 0.14 unit/mL in P. hydnoides, A. blazei, and Agaricus sp., respectively.

• Mycobiology
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• 제51권1호
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• pp.60-66
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• 2023

In this study, the α-amylase inhibitory activity, α-glucosidase inhibitory activity, pancreatic lipase inhibitory activity, and Xanthine Oxidase inhibitory activity of the fruiting body extracts of 5 varieties of Agaricus bisporus (AB) were confirmed. First, the α-amylase inhibitory activity of AB12, AB13, AB18, AB34, and AB40 methanol extracts was lower than that of acarbose, a positive control, in all concentration ranges. The α-glucosidase inhibitory activity of the AB40, AB13, and AB12 methanol extracts at the extract concentration of 1.0 mg/mL was 80.5%, 81.3%, and 78.5%, respectively, similar to that of acarbose, a positive control. The pancreatic lipase inhibitory activity of the methanol extract of Agaricus bisporus fruiting body was significantly lower than that of the positive control orlistat in the concentration range of 50~1.000 (mg/mL). The Xanthine Oxidase inhibitory activity was 0.5~8.0 mg/mL of each extract, which was significantly lower than that of the positive control allopurinol in the same concentration range. However, the Xanthine Oxidase inhibitory activity of AB13 and AB40 at 8.0 mg/mL was about 70%, which was higher than that of other mushrooms. In conclusion, five kinds of Agaricus bisporus fruiting bodies seem to have inhibitory effects on enzymes such as α-amylase, α-glucosidase, pancreatic lipase, and Xanthine Oxidase that degrade starch and protein. In particular, it has an inhibitory effect and a reduction effect on xanthine oxidase that causes gout, so it is expected that it can be developed and used as a food or health supplement with health functional properties through future research.

• 한국생물공학회:학술대회논문집
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• 한국생물공학회 2005년도 생물공학의 동향(XVI)
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• pp.99-102
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• 2005

The Basidomycetes fungus Agaricus blazei Murill has been well known as a health food for the prevention of cancer, diabetes, hyperlipidemia, arteriosclerosis and chronic hepatitis. This study was concentrated to investigate the characteristics of crude protein-bound polysaccharides(PBP) compositions extracted from Agaricus blazei Murill. In order to produce crude polysaccharides, culture conditions were examined using YMK media. Total sugars and protein contents of PBP were detected by phenol-sulfuric acid method and Bradford -assay. Hexosamine was found to be involved in the linkage, N-linked and O-linked types. To identify helical conformation existence, wavelength was measured using Congo red after the treatment with alkali solution.