• 복식문화연구
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• 제11권3호
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• pp.441-446
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• 2003

Water-insoluble chitosan with molecular weight of 2,000,000, 500,000, 80,000, and 40,000 and more than 90% of degree of deacetylation were used to test antibacterial activity of chitosan against a pathogenic bacteria, methicillin resistant Staphylococcus aureus(MRSA), which is being issued in the world. As experimental method, Agar plate Smear Method and Agar plate Contact Method were used. The moleculur weight of chitosan didn't exert significant influences on its antibacterial activity against MRSA but chitosan having molecular weight 40,000, 80,000 and 150,000 showed the excellent antibacterial activity. The antibacterial efHciency was excellent in applying it after chitosan was dissolved in acetic acid solution, while the antibacterial efficiency was not expressed nearly in case of applying after chitosan was dissolved in neutral water. Therefore, it is considered that chitosan can show the antibacterial efficiency only if a positive ion status of -NH₃/sup +/ is maintained. MIC of chitosan/acetic acid solution and cotton fabrics finished with chitosan/acetic acid solution showed in concentration of 0.05%.

• 한국식품과학회지
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• 제2권1호
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• pp.67-71
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• 1970

본 실험(本 實驗)에서는, amylase 활성(活性)을 측정(測定)하기 위하여 starch-agar-gel-plate를 만들어 cork borer로써 9mm의 구멍을 뚫고, 각(各) 농도(濃度)의 효소(酵素)를 주입(注入)하여 일정(一定)한 온도(溫度)에서 일정(一定)한 시간(時間)동만 반응(反應)시킨 후, 0.1N-iodine액(液)으로 분해(分解)하지 않은 starch를 착색시켜, 착색안된 무착색(無着色) zone의 직경을 측정(測定)해서 비교 검토한 것으로 서 이 방법(方法)을 "cork borrer method"라고 칭하였다. starch-agar-gel-plate 상(上)에서의 무착색(無着色) zone 형성(形成)의 최적(最適) pH는, Bacillus 균(菌)이 생산(生産)하는 amylase인 biotex, spitase 등은 중성(中性) 내지 알카리성(性)에서, 사상균이 생산(生産)하는 효소인 mucor-rennin, gluco-amylase, biodiase 등에 있어서는 pH $5{\sim}7$의 범위이다. 무착색(無着色) zone 형성(形成)직경은 전분농도(濃度)가 적을수록 유리하고, 반응시간(反應時間)이 길수록, amylase activity가 강할수록 커진다. 한편 반응온도(反應溫度)에 의한 영향은 $10^{\circ}C$보다 $50^{\circ}C$에서 무착색(無着色) zone이 크게 형성(形成)된다. 따라서 starch 온도(溫度) 및 pH가 일정(一定)한 starch-agar-gel-plate를 만들고, 반응시간(反應時間) 반응온도(反應溫度) 등을 일정하게 하여주면 amylase activity 를 능률있게 측정(測定)할 수 있다. 끝으로 본 연구(本 硏究)를 위하여 연구비(硏究費)를 지급(支給)하여주신 연합재단(聯合財團)과 대학원장 이길상박사(大學院長 李吉相博士), 또 효소(酵素)를 제공하여 주신 삼중효소공업주식회사(三中酵素工業株式會社)의 김동진사장(金東振社長). 천야제약주식회사(天野製藥株式會社)(일본(日本)) 등의 여러분께 진심으로 감사드립니다.

• 한국식품영양과학회지
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• 제31권4호
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• pp.589-593
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• 2002

유통 생씩 5종의 수분 함량 4.22~7.18%의 범위였으며 수분활성은 0.15~0.26의 분포를 보여 미생물에 의한 변패보다는 낮은 수분활성에서도 생존해 있는 병원성 미생물의 제거가 생식의 중요한 품질관리 요소일 것으로 판단된다. 생식의 미생물분포는 Bacillus group $10^4$~$10^{7}$ cfu/g, 사상균류 $10^2$~$10^3$cfu/g, coliform group $10^1$~ $10^4$cfu/g, SS agar plate 분리 enteric bacteria group $10^1$~$10^3$cfu/g의 수준으로 특히 병원성 미생물의 오염가능성이 컸다. 감마선 조사 결과 coliform group, SS agar plate 분리 enteric bacteria group, 그리고 사상균류는 3 kGy의 조사선량에서 완전살균 수준으로 제거되었다. 생식 분포 미생물의 D값은 coliform group은 0.68~0.80 kGy, SS agar plate 분리 enteric bacteria group은 0.59~0.74 kGy, Bacillus group은 1.84~2.18 kGy, 사상균류는 0.36~0.57 kGy의 범위를 나타내었다. 생식제품의 위생화를 목적으로 할 경우 감마선조사 선량은 생식제품에 분포하는 coliform group과 SS agar plate 분리 enteric bacteria group 미생물의 사멸기준인 3~5 kGy의 수준으로 설정하는 것이 바람직할 것으로 사료된다.

• Imaging Science in Dentistry
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• 제44권2호
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• pp.149-154
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• 2014

Purpose: This study assessed the effectiveness of three antimicrobial mouthrinses in reducing microbial growth on photostimulable phosphor (PSP) plates. Materials and Methods: Prior to performing a full-mouth radiographic survey (FMX), subjects were asked to rinse with one of the three test rinses ($Listerine^{(R)}$, $Decapinol^{(R)}$, or chlorhexidine oral rinse 0.12%) or to refrain from rinsing. Four PSP plates were sampled from each FMX through collection into sterile containers upon exiting the scanner. Flame-sterilized forceps were used to transfer the PSP plates onto blood agar plates (5% sheep blood agar). The blood agar plates were incubated at $37^{\circ}C$ for up to 72 h. An environmental control blood agar plate was incubated with each batch. Additionally, for control, 25 gas-sterilized PSP plates were plated onto blood agar and analyzed. Results: The mean number of bacterial colonies per plate was the lowest in the chlorhexidine group, followed by the Decapinol, Listerine, and the no rinse negative control groups. Only the chlorhexidine and Listerine groups were significantly different (p=0.005). No growth was observed for the 25 gas-sterilized control plates or the environmental control blood agar plates. Conclusion: The mean number of bacterial colonies was the lowest in the chlorhexidine group, followed by the Decapinol, Listerine, and the no rinse groups. Nonetheless, a statistically significant difference was found only in the case of Listerine. Additional research is needed to test whether a higher concentration (0.2%) or longer exposure period (two consecutive 30 s rinse periods) would be helpful in reducing PSP plate contamination further with chlorhexidine.

• Journal of Life Science
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• 제10권1호
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• pp.64-65
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• 2000

Insoluble phosphate-solubilizing bacteria are routinely screened by a plate assay method using Pikovskaya agar and a modified Pikovskaya medium. A modified Pikovskaya medium to improve the clarity of the yellow-colored halo has not necessarity improved the plate assay. Colonies of phosphate-solubilizing bacteria tested could be redily selected after 48 h of incubation by green-colored colony formation on plate in which bromcresol green(BCG) was included. Among them, two bacterial strains did not produce distinct yellow halos after 48 h of incubation. We suggest that the green colony formation on plate medium containing BCG is advantageous ofr rapid isolating phosphate-solubilizing bacteria directly from soil.

• 대한수의학회지
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• 제10권2호
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• pp.1-5
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• 1970

In these studies the efficracy of plate, tube agglutination and agar-gel precipitin test were compared for the detection of pullorum infected chickens. From all the chickens showing positive reaction in agar-gel precipitin test, Salmonella pullorum organisms were isolated.

• KSBB Journal
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• 제13권4호
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• pp.378-382
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• 1998

Agar hydorlysates or agarooligosaccharides from agar prepared by Cytophaga agarase showed eight spots on TLC plate and the degree of polymerization of the spots were in the reange of 2.5 - 6.5. Each component of the hydrolysate as tested the several functionalities such as antimicrobial activity, anticavity activity, and anticoagulant activity. The anticativity activity and anticoagulant acitvity were found in all fractions of hydrolysates and several spot on TLC, whereas the anticoagulant activity was very low.

• 한국식품위생안전성학회지
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• 제19권4호
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• pp.209-216
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• 2004

우리나라 전통식품인 김치, 된장, 고추장, 간장. 탁주, 식혜, 수정과에 있는 미생물 분석을 위하여 건조필름법과 전통적인 미생물 분석법을 비교하였다. 일반세균 분석에는 plate count agar 법과 $Petrifilm^{TM}$ aerobic count plate 법을 비교하였고, 대장균군과 대장균의 분석에는 most probable number (MPN) 법과 $Petrifilm^{TM}$ coliform count plate 및 $Petrifilm^{TM}$ E. coli/colmfom count plate 법을 각각 비교하였으며, 효모와 곰팡이의 분석에는 potato dextrose agar 법과 $Petrifilm^{TM}$ yeast and mold count plate 법을 비교하였다. 황색포도상구균의 분석에는 coagulase 시험법과 $Petrifilm^{TM}$ Staph express fount plate 법을 비교하였다. 두 방법간의 상관계수는 일반세균이 0.974-0.998, 대장균군이 0.955-0.978, 대장균이 0.968-0.986, 효모와 곰팡이가 0.913-0.995, 황색포도상구균이 0.998-0.999로 두 방법간의 상관성이 매우 높았으며, 평균 미생물수에 있어서도 유의적인 차이가 없었다(p>0.05). 이러한 결과로부터 건조필름법이 기존의 전통적인 방법을 대체할 수 있는 미생물 분석법임이 확인되었다.

• Journal of Oral Medicine and Pain
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• 제23권4호
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• pp.343-352
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• 1998

This experiment was performed to investigate effects of zinc containing solution on the major normal flora Staphylococccus aureus, Streptococus mutans and Candida albicans and to observe the variation according to anionic change and concentration difference. Zinc chloride, zinc iodide and zinc acetate solution were added to werially diluted broth culture so that each final concentration might be 0.25%, 0.5%. 1%. After that, 100ul of each aliquot was spreaded on each selective media plate( Mannitol Salts Agar plate for Staphylococcus aureus, Mitis Salivarius Agar plate for Streptococcus mutans and Sabouraud Destrose Agar plate for Candida albicans). The % killing was calculated bu CFU count after incubation under the appropriate condition. 1. zinc iodide, zinc chloride, and zinc acetate solutions showed inhibitory effects on Staphylococcus aureus, Streptococcus mutans and Candida albicans. 2. The inhibitory effects on Staphylococcus aureus were ranked in order of ainc iodide, zinc chloride and zinc actate. 3. The inhibitory effects on Streptococcus mutans were ranked in orfer of zinc iodide, zinc chloride and zinc acetate. 4. the inhibitory effects on Candida albicans showed no difference among zinc iodide, zinc chloride and zinc acetate. 5. The inhibitory effects of zinc chloride and zinc acetate on Staphylococcus aureus and Streptococcus mutnas showed increasing pattern as the concentration increase. But the inhibitory effects of zinc iodide on Staphylococcus aureus and Streptococcus mutans showed no apparent difference according to concentrations and it was the case with the inhibitory effects of zinc iodide, zinc chloride and zinc acetate on Candida albicans.

• Preventive Nutrition and Food Science
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• 제18권4호
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• pp.269-272
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• 2013

This study was performed to compare the performance of Sanita-Kun dry medium culture plate with those of traditional culture medium and Petrifilm dry medium culture plate for the enumeration of the mesophilic aerobic bacteria in milk, ice cream, ham, and codfish fillet. Mesophilic aerobic bacteria were comparatively evaluated in milk, ice cream, ham, and codfish fillet using Sanita-Kun aerobic count (SAC), Petrifilm aerobic count (PAC), and traditional plate count agar (PCA) media. According to the results, all methods showed high correlations of 0.989~1.000 and no significant differences were observed for enumerating the mesophilic aerobic bacteria in the tested food products. SAC method was easier to perform and count colonies efficiently as compared to the PCA and PAC methods. Therefore, we concluded that the SAC method offers an acceptable alternative to the PCA and PAC methods for counting the mesophilic aerobic bacteria in milk, ice cream, ham, and codfish fillet products.